LOCUS       RC204185                8586 bp    DNA     circular  SYN 
DEFINITION  PHKA2 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 2 (liver) (PHKA2)
ACCESSION   NM_000292
VERSION     NM_000292.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="PHKA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGAGCAGG AGCAATTCCG GGGTCCGCTT GGACGGGTAC GCGCGGCTGG 
     1081 TGCAGCAAAC CATCCTGTGT TACCAGAATC CCGTCACGGG GCTGCTGTCA GCCAGCCATG 
     1141 AGCAGAAGGA TGCCTGGGTG CGGGATAACA TCTACAGTAT CCTGGCCGTG TGGGGCCTGG 
     1201 GCATGGCCTA CCGTAAGAAT GCAGACCGCG ATGAGGACAA GGCCAAGGCC TACGAGCTGG 
     1261 AGCAGAACGT GGTGAAGCTG ATGCGAGGTC TTCTCCAGTG CATGATGAGA CAGGTGGCCA 
     1321 AAGTGGAGAA GTTCAAACAC ACTCAGAGCA CCAAGGACAG CCTGCACGCC AAGTACAACA 
     1381 CCGCCACCTG TGGCACGGTG GTGGGCGACG ACCAGTGGGG CCACCTCCAG GTGGATGCCA 
     1441 CCTCTCTCTT CCTCCTGTTC CTGGCCCAGA TGACCGCCTC AGGCTTACGT ATCATTTTCA 
     1501 CTCTCGATGA GGTGGCCTTC ATACAGAATC TTGTCTTTTA CATAGAAGCT GCATATAAAG 
     1561 TCGCTGATTA TGGAATGTGG GAGCGTGGAG ATAAGACTAA TCAGGGCATC CCGGAATTGA 
     1621 ATGCAAGCTC CGTAGGAATG GCCAAGGCAG CTCTTGAGGC AATTGATGAA CTGGACCTTT 
     1681 TTGGAGCCCA TGGAGGACGC AAGTCAGTGA TTCATGTTCT GCCAGATGAG GTCGAGCACT 
     1741 GCCAGTCTAT TCTGTTCTCC ATGCTGCCAA GAGCGTCGAC ATCTAAAGAA ATTGATGCTG 
     1801 GACTTCTTTC CATTATTTCC TTCCCGGCCT TTGCAGTGGA AGATGTAAAC CTTGTAAATG 
     1861 TGACCAAAAA TGAAATTATT TCTAAGCTCC AGGGGCGTTA TGGATGCTGT CGCTTCCTTC 
     1921 GAGATGGTTA TAAAACTCCA AGAGAGGACC CTAATCGACT GCATTATGAC CCTGCTGAAC 
     1981 TCAAGCTCTT CGAAAACATT GAATGTGAGT GGCCTGTGTT TTGGACATAT TTTATAATAG 
     2041 ATGGAGTCTT CAGTGGTGAT GCTGTTCAGG TCCAAGAATA CCGAGAGGCC CTGGAGGGAA 
     2101 TACTCATCAG AGGCAAGAAT GGGATCCGCC TGGTGCCTGA ACTCTACGCT GTCCCGCCTA 
     2161 ACAAGGTAGA TGAAGAGTAC AAGAATCCTC ACACAGTAGA CCGAGTTCCT ATGGGGAAGG 
     2221 TGCCTCATCT GTGGGGCCAA TCCTTGTACA TCCTCAGCTC GCTGTTGGCA GAGGGATTCC 
     2281 TTGCCGCTGG TGAAATCGAT CCCTTAAATA GAAGATTTTC CACTTCAGTC AAACCTGATG 
     2341 TTGTAGTACA AGTTACTGTT TTGGCAGAAA ACAATCACAT TAAGGACTTA TTGAGGAAAC 
     2401 ACGGGGTGAA CGTCCAGAGT ATCGCGGACA TTCATCCAAT TCAAGTCCAG CCGGGCCGGA 
     2461 TTCTTAGTCA CATATATGCC AAGCTTGGAC GGAATAAGAA TATGAATTTG AGTGGGCGAC 
     2521 CGTATCGACA TATTGGTGTC CTTGGAACCT CTAAACTATA TGTGATTAGG AACCAAATCT 
     2581 TTACTTTTAC ACCCCAGTTC ACCGACGAGC ATCACTTCTA CCTGGCCCTC GACAATGAGA 
     2641 TGATCGTGGA GATGCTAAGG ATCGAGCTGG CCTACCTGTG CACCTGCTGG AGGATGACGG 
     2701 GCAGACCCAC ACTCACCTTC CCCATCAGTC GCACCATGCT CACAAATGAT GGCTCAGACA 
     2761 TTCATTCTGC TGTGCTCTCC ACAATTAGAA AACTAGAGGA TGGATATTTT GGAGGAGCCA 
     2821 GAGTAAAATT AGGGAACCTT TCGGAATTTC TCACCACATC GTTCTACACA TATCTGACTT 
     2881 TTCTGGATCC AGACTGTGAT GAGAAGTTGT TTGACAATGC CAGCGAAGGG ACTTTCAGTC 
     2941 CTGATAGTGA TTCAGATTTG GTAGGATATC TGGAAGACAC CTGTAATCAA GAAAGCCAAG 
     3001 ACGAACTTGA CCATTATATC AACCACCTTC TGCAAAGCAC ATCGTTGAGG TCCTATCTGC 
     3061 CTCCTCTTTG TAAGAACACA GAAGACCGCC ATGTCTTCAG TGCTATCCAC TCCACGCGGG 
     3121 ACATACTTTC TGTGATGGCA AAAGCAAAGG GTTTGGAAGT TCCATTTGTT CCCATGACTT 
     3181 TGCCGACTAA AGTTCTAAGT GCCCACCGTA AATCACTGAA TCTTGTTGAT TCTCCTCAGC 
     3241 CACTCCTAGA AAAGGTTCCT GAAAGTGACT TTCAGTGGCC CAGAGATGAC CATAGTGACG 
     3301 TGGACTGTGA GAAGCTGGTT GAGCAGCTAA AAGATTGTTC GAACCTACAG GACCAAGCAG 
     3361 ACATTCTGTA CATTCTTTAT GTCATAAAGG GTCCCAGCTG GGACACAAAT CTCTCTGGAC 
     3421 AGCACGGGGT CACCGTTCAA AACCTTCTTG GTGAGCTCTA TGGGAAAGCC GGCTTGAACC 
     3481 AGGAGTGGGG TCTGATTCGC TACATCTCAG GCCTTCTCAG GAAGAAAGTG GAGGTCCTGG 
     3541 CTGAGGCCTG CACAGACCTG CTTTCGCACC AGAAGCAGCT CACCGTGGGC CTGCCGCCCG 
     3601 AGCCCCGGGA GAAGATCATC TCTGCGCCCC TTCCCCCAGA GGAGCTCACA AAACTCATCT 
     3661 ACGAGGCCAG TGGGCAGGAC ATCAGCATTG CCGTCCTCAC GCAGGAGATT GTGGTTTACC 
     3721 TGGCCATGTA TGTCAGGGCG CAGCCCAGCC TCTTTGTGGA GATGCTGAGA CTCCGGATTG 
     3781 GACTGATCAT TCAGGTGATG GCCACGGAGC TGGCACGGAG CCTGAACTGC TCAGGAGAAG 
     3841 AGGCTTCTGA AAGTTTGATG AACCTCAGCC CTTTCGATAT GAAAAATCTC CTGCACCATA 
     3901 TTCTAAGTGG GAAAGAGTTT GGCGTTGAAA GAAGTGTGCG CCCTATCCAC TCCTCCACAT 
     3961 CCAGCCCTAC CATCTCCATC CACGAGGTGG GCCATACCGG AGTCACCAAA ACTGAGAGGA 
     4021 GTGGCATTAA CAGACTGAGG AGTGAAATGA AACAGATGAC TAGGCGGTTT AGTGCTGATG 
     4081 AACAGTTCTT TTCTGTGGGC CAGGCCGCGT CCAGCAGTGC GCATTCCTCC AAGTCTGCGA 
     4141 GGTCCAGCAC CCCATCCTCG CCCACTGGCA CGTCATCCTC AGACTCGGGA GGACATCACA 
     4201 TCGGCTGGGG TGAGCGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAT GGGGCCATCA 
     4261 ACAGGGTCCC CGTGGGATTC TACCAGAGGG TGTGGAAGAT CCTCCAGAAG TGCCACGGTC 
     4321 TCTCCATCGA TGGTTATGTC CTCCCATCCT CGACGACCCG AGAGATGACC CCGCATGAGA 
     4381 TCAAGTTTGC TGTCCATGTC GAATCGGTGC TGAACCGCGT GCCGCAGCCC GAGTACCGGC 
     4441 AGCTGCTGGT GGAAGCCATC ATGGTGCTGA CGCTGCTCTC GGACACGGAG ATGACCAGCA 
     4501 TCGGGGGCAT CATCCACGTG GACCAGATCG TGCAGATGGC CAGTCAGCTG TTCTTGCAGG 
     4561 ACCAGGTGTC AATTGGTGCC ATGGACACCC TGGAGAAAGA CCAAGCCACA GGAATCTGCC 
     4621 ACTTCTTTTA TGACAGCGCT CCGAGTGGGG CTTATGGGAC GATGACCTAC CTAACAAGAG 
     4681 CAGTGGCTTC TTATTTGCAG GAATTGTTGC CCAATTCGGG CTGCCAGATG CAAACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//