LOCUS       RC204117                8097 bp    DNA     circular  SYN 
DEFINITION  RNF31 (Myc-DDK-tagged)-Human ring finger protein 31 (RNF31)
ACCESSION   NM_017999
VERSION     NM_017999.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4254..4345 
                     /label="Myc-DDK"  
     misc_feature    4375..4960 
                     /label="PolyA signal"  
     misc_feature    4432..4451 
                     /label="Restriction Enzyme"  
     misc_feature    5236..5908 
                     /label="ColE1"  
     misc_feature    6338..7132 
                     /label="Kan/Neo"  
     misc_feature    7167..7469 
                     /label="SV40 ori"  
     misc_feature    7555..8032 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4236..4242 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4236 
                     /label="RNF31"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGGGGAG GAAGAGGAGC GGGCCTTCCT GGTGGCCCGC GAGGAGCTGG 
     1081 CGAGCGCCCT GAGGAGGGAT TCCGGGCAGG CGTTTTCCCT GGAGCAGCTC CGGCCGCTAC 
     1141 TAGCCAGCTC TCTGCCGCTA GCCGCCCGCT ACCTGCAGCT GGACGCCGCA CGCCTTGTCC 
     1201 GCTGCAACGC TCATGGGGAG CCCCGAAACT ACCTCAACAC CCTGTCCACG GCTCTGAACA 
     1261 TCCTGGAGAA ATACGGCCGC AACCTTCTCA GCCCTCAGCG GCCTCGGTAC TGGCGTGGTG 
     1321 TCAAGTTTAA TAACCCTGTC TTTCGCAGCA CGGTGGATGC TGTGCAGGGG GGCCGAGATG 
     1381 TGCTGCGATT ATATGGCTAC ACAGAGGAGC AACCAGATGG GTTGAGCTTC CCCGAAGGGC 
     1441 AGGAGGAGCC AGATGAGCAC CAGGTTGCTA CAGTCACACT GGAAGTACTG CTGCTTCGGA 
     1501 CAGAGCTCAG CCTGCTATTG CAGAATACTC ATCCAAGACA GCAGGCACTG GAGCAGCTGT 
     1561 TGGAAGACAA GGTTGAAGAT GATATGCTGC AGCTTTCAGA ATTTGACCCC CTATTGAGAG 
     1621 AGATTGCTCC TGGCCCCCTC ACCACACCCT CTGTCCCAGG CTCCACTCCT GGTCCCTGCT 
     1681 TCCTCTGTGG TTCTGCCCCA GGCACACTGC ACTGCCCATC CTGTAAACAG GCCCTGTGTC 
     1741 CAGCCTGTGA CCACCTGTTC CATGGACACC CATCCCGTGC TCATCACCTC CGCCAGACCC 
     1801 TGCCTGGGGT CCTGCAGGGT ACCCACCTGA GCCCCAGTTT ACCTGCCTCA GCCCAACCAC 
     1861 GGCCCCAGTC GACCTCCCTG CTGGCCCTGG GAGACAGCTC TCTTTCTTCC CCTAATCCTG 
     1921 CAAGTGCTCA TTTGCCCTGG CACTGTGCTG CCTGTGCCAT GCTAAATGAG CCTTGGGCAG 
     1981 TGCTCTGTGT GGCCTGTGAT CGGCCCCGAG GCTGTAAGGG GTTGGGGTTG GGAACTGAGG 
     2041 GTCCCCAAGG AACTGGAGGC CTAGAACCTG ATCTTGCACG GGGTCGGTGG GCCTGCCAGA 
     2101 GCTGTACCTT TGAGAATGAG GCAGCTGCTG TGCTATGTTC CATATGTGAG CGACCTCGGC 
     2161 TGGCCCAGCC TCCCAGCTTG GTGGTGGATT CCCGAGATGC TGGCATTTGC CTGCAACCCC 
     2221 TTCAGCAGGG GGATGCTTTG CTGGCCTCTG CCCAGAGTCA AGTCTGGTAC TGTATTCACT 
     2281 GTACCTTCTG CAACTCGAGC CCTGGCTGGG TGTGTGTTAT GTGCAACCGG ACTAGTAGCC 
     2341 CCATTCCAGC ACAACATGCC CCCCGGCCCT ATGCCAGCTC TTTGGAAAAG GGACCCCCCA 
     2401 AGCCTGGGCC CCCACGACGC CTTAGTGCCC CCCTGCCCAG TTCCTGTGGA GATCCTGAGA 
     2461 AGCAGCGCCA AGACAAGATG CGGGAAGAAG GCCTCCAGCT AGTGAGCATG ATCCGGGAAG 
     2521 GGGAAGCCGC AGGTGCCTGT CCAGAGGAGA TCTTCTCGGC TCTGCAGTAC TCGGGCACTG 
     2581 AGGTGCCTCT GCAGTGGTTG CGCTCAGAAC TGCCCTACGT CCTGGAGATG GTGGCTGAGC 
     2641 TGGCTGGACA GCAGGACCCT GGGCTGGGTG CCTTTTCCTG TCAGGAGGCC CGGAGAGCCT 
     2701 GGCTGGATCG TCATGGCAAC CTTGATGAAG CTGTGGAGGA GTGTGTGAGG ACCAGGCGAA 
     2761 GGAAGGTGCA GGAGCTCCAG TCTCTAGGCT TTGGGCCTGA GGAGGGGTCT CTCCAGGCAT 
     2821 TGTTCCAGCA CGGAGGTGAT GTGTCACGGG CCCTGACTGA GCTACAGCGC CAACGCCTAG 
     2881 AGCCCTTCCG CCAGCGCCTC TGGGACAGTG GCCCTGAGCC CACCCCTTCC TGGGATGGGC 
     2941 CAGACAAGCA GAGCCTGGTC AGGCGGCTTT TGGCAGTCTA CGCACTCCCC AGCTGGGGCC 
     3001 GGGCAGAGCT GGCACTGTCA CTGCTGCAGG AGACACCCAG GAACTATGAG TTGGGGGATG 
     3061 TGGTAGAAGC TGTGAGGCAC AGCCAGGACC GGGCCTTCCT GCGCCGCTTG CTTGCCCAGG 
     3121 AGTGTGCCGT GTGTGGCTGG GCCCTGCCCC ACAACCGGAT GCAGGCCCTG ACTTCCTGTG 
     3181 AGTGCACCAT CTGTCCTGAC TGCTTCCGCC AGCACTTCAC CATCGCCTTG AAGGAGAAGC 
     3241 ACATCACAGA CATGGTGTGC CCTGCCTGTG GCCGCCCCGA CCTCACCGAT GACACACAGT 
     3301 TGCTCAGCTA CTTCTCTACC CTTGACATCC AGCTTCGCGA GAGCCTAGAG CCAGATGCCT 
     3361 ATGCGTTGTT CCATAAGAAG CTGACCGAGG GTGTGCTGAT GCGGGACCCC AAGTTCTTGT 
     3421 GGTGTGCCCA GTGCTCCTTT GGCTTCATAT ATGAGCGTGA GCAGCTGGAG GCAACTTGTC 
     3481 CCCAGTGTCA CCAGACCTTC TGTGTGCGCT GCAAGCGCCA GTGGGAGGAG CAGCACCGAG 
     3541 GTCGGAGCTG TGAGGACTTC CAGAACTGGA AACGCATGAA CGACCCAGAA TACCAGGCCC 
     3601 AGGGCCTAGC AATGTATCTT CAGGAAAACG GCATTGACTG CCCCAAATGC AAGTTCTCGT 
     3661 ACGCCCTGGC CCGAGGAGGC TGCATGCACT TTCACTGTAC CCAGTGCCGC CACCAGTTCT 
     3721 GCAGCGGCTG CTACAATGCC TTTTACGCCA AGAATAAATG TCCAGAGCCT AACTGCAGGG 
     3781 TGAAAAAGTC CCTGCACGGC CACCACCCTC GAGACTGCCT CTTCTACCTG CGGGACTGGA 
     3841 CTGCTCTCCG GCTTCAGAAG CTGCTACAGG ACAATAACGT CATGTTTAAT ACAGAGCCTC 
     3901 CAGCTGGGGC CCGGGCAGTC CCTGGAGGCG GCTGCCGAGT GATAGAGCAG AAGGAGGTTC 
     3961 CCAATGGGCT CAGGGACGAA GCTTGTGGCA AGGAAACTCC AGCTGGCTAT GCCGGCCTGT 
     4021 GCCAGGCACA CTACAAAGAG TATCTTGTGA GCCTCATCAA TGCCCACTCG CTGGACCCAG 
     4081 CCACCTTGTA TGAGGTGGAA GAGCTGGAGA CGGCCACTGA GCGCTACCTG CACGTACGCC 
     4141 CCCAGCCTTT GGCTGGAGAG GATCCCCCTG CTTACCAGGC CCGCTTGTTA CAGAAGCTGA 
     4201 CAGAAGAGGT ACCCTTGGGA CAGAGTATCC CCCGCAGGCG GAAGACGCGT ACGCGGCCGC 
     4261 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4321 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4381 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4441 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4501 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4561 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4621 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4681 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4741 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4801 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4861 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4921 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4981 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5041 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5101 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5881 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5941 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6001 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6061 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6121 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6181 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6241 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6301 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6361 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6421 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6481 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6541 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6601 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6661 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6721 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6781 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6841 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6901 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6961 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7021 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7081 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7141 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7201 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7261 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7321 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7441 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7501 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7561 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7621 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7681 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7741 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7801 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7861 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7921 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7981 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8041 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//