LOCUS       RC204099L1                9234 bp    DNA     circular  SYN 
DEFINITION  CYLD (Myc-DDK-tagged)-Human cylindromatosis (turban tumor syndrome) (CYLD), transcript variant 2
ACCESSION   NM_001042355
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7525..7705 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8433..9092 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7864..8432 
                     /label="ColE1"  
     misc_feature    6400..6420 
                     /label="LR50 primer"  
     misc_feature    6197..6268 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6409..7000 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6179..6185 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6179 
                     /label="CYLD"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGTTCAGGC TTATGGAGCC AAGAAAAAGT 
     3361 CACTTCACCC TACTGGGAAG AGCGGATTTT TTACTTGCTT CTTCAAGAAT GCAGCGTTAC 
     3421 AGACAAACAA ACACAAAAGC TCCTTAAAGT ACCGAAGGGA AGTATAGGAC AGTATATTCA 
     3481 AGATCGTTCT GTGGGGCATT CAAGGATTCC TTCTGCAAAA GGCAAGAAAA ATCAGATTGG 
     3541 ATTAAAAATT CTAGAGCAAC CTCATGCAGT TCTCTTTGTT GATGAAAAGG ATGTTGTAGA 
     3601 GATAAATGAA AAGTTCACAG AGTTACTTTT GGCAATTACC AATTGTGAGG AGAGGTTCAG 
     3661 CCTGTTTAAA AACAGAAACA GACTAAGTAA AGGCCTCCAA ATAGACGTGG GCTGTCCTGT 
     3721 GAAAGTACAG CTGAGATCTG GGGAAGAAAA ATTTCCTGGA GTTGTACGCT TCAGAGGACC 
     3781 CCTGTTAGCA GAGAGGACAG TCTCCGGAAT ATTCTTTGGA GTTGAATTGC TGGAAGAAGG 
     3841 TCGTGGTCAA GGTTTCACTG ACGGGGTGTA CCAAGGGAAA CAGCTTTTTC AGTGTGATGA 
     3901 AGATTGTGGC GTGTTTGTTG CATTGGACAA GCTAGAACTC ATAGAAGATG ATGACACTGC 
     3961 ATTGGAAAGT GATTACGCAG GTCCTGGGGA CACAATGCAG GTCGAACTTC CTCCTTTGGA 
     4021 AATAAACTCC AGAGTTTCTT TGAAGGTTGG AGAAACAATA GAATCTGGAA CAGTTATATT 
     4081 CTGTGATGTT TTGCCAGGAA AAGAAAGCTT AGGATATTTT GTTGGTGTGG ACATGGATAA 
     4141 CCCTATTGGC AACTGGGATG GAAGATTTGA TGGAGTGCAG CTTTGTAGTT TTGCGTGTGT 
     4201 TGAAAGTACA ATTCTATTGC ACATCAATGA TATCATCCCA GAGAGTGTGA CGCAGGAAAG 
     4261 GAGGCCTCCC AAACTTGCCT TTATGTCAAG AGGTGTTGGG GACAAAGGTT CATCCAGTCA 
     4321 TAATAAACCA AAGGCTACAG GATCTACCTC AGACCCTGGA AATAGAAACA GATCTGAATT 
     4381 ATTTTATACC TTAAATGGGT CTTCTGTTGA CTCACAACCA CAATCCAAAT CAAAAAATAC 
     4441 ATGGTACATT GATGAAGTTG CAGAAGACCC TGCAAAATCT CTTACAGAGA TATCTACAGA 
     4501 CTTTGACCGT TCTTCACCAC CACTCCAGCC TCCTCCTGTG AACTCACTGA CCACCGAGAA 
     4561 CAGATTCCAC TCTTTACCAT TCAGTCTCAC CAAGATGCCC AATACCAATG GAAGTATTGG 
     4621 CCACAGTCCA CTTTCTCTGT CAGCCCAGTC TGTAATGGAA GAGCTAAACA CTGCACCCGT 
     4681 CCAAGAGAGT CCACCCTTGG CCATGCCTCC TGGGAACTCA CATGGTCTAG AAGTGGGCTC 
     4741 ATTGGCTGAA GTTAAGGAGA ACCCTCCTTT CTATGGGGTA ATCCGTTGGA TCGGTCAGCC 
     4801 ACCAGGACTG AATGAAGTGC TCGCTGGACT GGAACTGGAA GATGAGTGTG CAGGCTGTAC 
     4861 GGATGGAACC TTCAGAGGCA CTCGGTATTT CACCTGTGCC CTGAAGAAGG CGCTGTTTGT 
     4921 GAAACTGAAG AGCTGCAGGC CTGACTCTAG GTTTGCATCA TTGCAGCCGG TTTCCAATCA 
     4981 GATTGAGCGC TGTAACTCTT TAGCATTTGG AGGCTACTTA AGTGAAGTAG TAGAAGAAAA 
     5041 TACTCCACCA AAAATGGAAA AAGAAGGCTT GGAGATAATG ATTGGGAAGA AGAAAGGCAT 
     5101 CCAGGGTCAT TACAATTCTT GTTACTTAGA CTCAACCTTA TTCTGCTTAT TTGCTTTTAG 
     5161 TTCTGTTCTG GACACTGTGT TACTTAGACC CAAAGAAAAG AACGATGTAG AATATTATAG 
     5221 TGAAACCCAA GAGCTACTGA GGACAGAAAT TGTTAATCCT CTGAGAATAT ATGGATATGT 
     5281 GTGTGCCACA AAAATTATGA AACTGAGGAA AATACTTGAA AAGGTGGAGG CTGCATCAGG 
     5341 ATTTACCTCT GAAGAAAAAG ATCCTGAGGA ATTCTTGAAT ATTCTGTTTC ATCATATTTT 
     5401 AAGGGTAGAA CCTTTGCTAA AAATAAGATC AGCAGGTCAA AAGGTACAAG ATTGTTACTT 
     5461 CTATCAAATT TTTATGGAAA AAAATGAGAA AGTTGGCGTT CCCACAATTC AGCAGTTGTT 
     5521 AGAATGGTCT TTTATCAACA GTAACCTGAA ATTTGCAGAG GCACCATCAT GTCTGATTAT 
     5581 TCAGATGCCT CGATTTGGAA AAGACTTTAA ACTATTTAAA AAAATTTTTC CTTCTCTGGA 
     5641 ATTAAATATA ACAGATTTAC TTGAAGACAC TCCCAGACAG TGCCGGATAT GTGGAGGGCT 
     5701 TGCAATGTAT GAGTGTAGAG AATGCTACGA CGATCCGGAC ATCTCAGCTG GAAAAATCAA 
     5761 GCAGTTTTGT AAAACCTGCA ACACTCAAGT CCACCTTCAT CCGAAGAGGC TGAATCATAA 
     5821 ATATAACCCA GTGTCACTTC CCAAAGACTT ACCCGACTGG GACTGGAGAC ACGGCTGCAT 
     5881 CCCTTGCCAG AATATGGAGT TATTTGCTGT TCTCTGCATA GAAACAAGCC ACTATGTTGC 
     5941 TTTTGTGAAG TATGGGAAGG ACGATTCTGC CTGGCTCTTC TTTGACAGCA TGGCCGATCG 
     6001 GGATGGTGGT CAGAATGGCT TCAACATTCC TCAAGTCACC CCATGCCCAG AAGTAGGAGA 
     6061 GTACTTGAAG ATGTCTCTGG AAGACCTGCA TTCCTTGGAC TCCAGGAGAA TCCAAGGCTG 
     6121 TGCACGAAGA CTGCTTTGTG ATGCATATAT GTGCATGTAC CAGAGTCCAA CAATGAGTTT 
     6181 GTACAAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC 
     6241 AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCT 
     6301 GTACAAGTAG GATTCGTCGA GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT 
     6361 ATACATGTTT AAGGGTTCCG GTTCCACTAG GTACAATTCG ATATCAAGCT TATCGATAAT 
     6421 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT 
     6481 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG 
     6541 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG 
     6601 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT 
     6661 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT 
     6721 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG 
     6781 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC 
     6841 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT 
     6901 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC 
     6961 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA 
     7021 CCTCGATCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA 
     7081 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC 
     7141 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA 
     7201 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT 
     7261 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG 
     7321 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA 
     7381 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG 
     7441 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA 
     7501 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 
     7561 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 
     7621 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 
     7681 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGCATG TGAGCAAAAG GCCAGCAAAA 
     7741 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     7801 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     7861 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     7921 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     7981 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     8041 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     8101 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     8161 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     8221 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     8281 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     8341 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     8401 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG ATTACGCCCC GCCCTGCCAC 
     8461 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 
     8521 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 
     8581 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG 
     8641 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG 
     8701 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC 
     8761 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA 
     8821 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA 
     8881 CGGAACTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 
     8941 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 
     9001 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 
     9061 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TACTCTTCCT TTTTCAATAT 
     9121 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     9181 AAAAATAAAC AAATAGGGGT CCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAC
//