LOCUS       RC203870L4                9563 bp    DNA     circular  SYN 
DEFINITION  VPS33B (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B)
ACCESSION   NM_018668
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7854..8034 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8762..9408 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6729..6749 
                     /label="LR50"  
     misc_feature    5192..5920 
                     /label="mGFP"  
     misc_feature    5927..5992 
                     /label="P2A"  
     misc_feature    8082..8701 
                     /label="pBR322 Ori"  
     misc_feature    5993..6592 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5171..5177 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5171 
                     /label="VPS33B"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCTTTTCCC CATCGGCCGG ACGCCCCTGA 
     3361 GCTGCCTGAC TTCTCCATGC TGAAGAGGCT GGCTCGAGAC CAGCTCATCT ATCTGCTGGA 
     3421 GCAGCTTCCT GGAAAAAAGG ATTTATTCAT TGAGGCAGAT CTCATGAGCC CTTTGGATCG 
     3481 AATTGCCAAT GTCTCCATCC TGAAGCAACA CGAAGTAGAC AAGCTATACA AGGTGGAGAA 
     3541 CAAGCCAGCC CTCAGCTCCA ATGAACAATT GTGCTTCTTG GTCAGACCCC GCATCAAGAA 
     3601 TATGCGATAC ATTGCCAGTC TTGTCAATGC TGACAAATTG GCTGGCCGAA CTCGCAAATA 
     3661 CAAAGTGATC TTCAGCCCTC AAAAGTTCTA TGCGTGTGAG ATGGTGCTTG AGGAAGAGGG 
     3721 AATCTATGGA GATGTGAGCT GTGATGAATG GGCCTTCTCT TTGCTGCCTC TTGATGTGGA 
     3781 TCTGCTGAGC ATGGAACTAC CAGAATTTTT CAGGGATTAC TTTCTGGAAG GAGATCAGCG 
     3841 TTGGATCAAC ACTGTAGCTC AGGCCTTACA CCTTCTCAGC ACTCTCTATG GACCCTTTCC 
     3901 AAACTGCTAT GGAATTGGCA GGTGCGCCAA GATGGCATAT GAATTGTGGA GGAACCTGGA 
     3961 GGAGGAGGAG GATGGCGAAA CCAAGGGCCG AAGGCCAGAG ATTGGACATA TCTTTCTCTT 
     4021 GGACAGAGAT GTGGACTTTG TGACAGCACT TTGCTCCCAA GTGGTTTATG AGGGCCTAGT 
     4081 AGATGACACC TTCCGCATCA AGTGTGGGAG TGTCGACTTT GGCCCAGAAG TCACATCCTC 
     4141 TGACAAGAGC CTGAAGGTGC TACTCAATGC CGAGGACAAG GTGTTTAATG AGATTCGGAA 
     4201 CGAGCACTTC TCCAATGTCT TTGGCTTCTT GAGCCAGAAG GCCCGGAACT TGCAGGCCCA 
     4261 GTATGATCGC CGGAGAGGCA TGGACATTAA GCAGATGAAG AATTTCGTGT CCCAGGAGCT 
     4321 CAAGGGCCTG AAACAGGAGC ACCGCCTGCT GAGTCTCCAT ATTGGGGCCT GTGAATCCAT 
     4381 CATGAAGAAG AAAACCAAGC AGGATTTCCA GGAGCTAATC AAGACTGAGC ATGCACTGCT 
     4441 AGAGGGGTTC AACATCCGGG AGAGCACCAG CTACATTGAG GAACACATAG ACCGGCAGGT 
     4501 GTCGCCTATA GAAAGCCTGC GCCTCATGTG CCTTTTGTCC ATCACTGAGA ATGGTTTGAT 
     4561 CCCCAAGGAT TACCGATCTC TGAAAACACA GTATCTGCAG AGCTATGGCC CTGAGCACCT 
     4621 GCTAACCTTC TCCAATCTGC GAAGAGCTGG GCTCCTAACG GAGCAGGCCC CCGGGGACAC 
     4681 CCTCACAGCC GTGGAGAGTA AAGTGAGCAA GCTGGTGACC GACAAGGCTG CAGGAAAGAT 
     4741 TACTGATGCC TTCAGTTCTC TGGCCAAGAG GAGCAATTTT CGTGCCATCA GCAAAAAGCT 
     4801 GAATTTGATC CCACGTGTGG ACGGCGAGTA TGATCTGAAA GTGCCCCGAG ACATGGCTTA 
     4861 CGTCTTCAGT GGTGCTTATG TGCCCCTGAG CTGCCGAATC ATTGAGCAGG TGCTAGAGCG 
     4921 GCGAAGCTGG CAGGGCCTTG ATGAGGTGGT ACGGCTGCTC AACTGCAGTG ACTTTGCATT 
     4981 CACAGATATG ACTAAGGAAG ACAAGGCTTC CAGTGAGTCC CTGCGCCTCA TCTTGGTGGT 
     5041 GTTCTTGGGT GGTTGTACAT TCTCTGAGAT CTCAGCCCTC CGGTTCCTGG GCAGAGAGAA 
     5101 AGGCTACAGG TTCATTTTCC TGACGACAGC AGTCACAAAC AGCGCTCGCC TTATGGAGGC 
     5161 CATGAGTGAG GTGAAAGCCA CGCGTACGCG GCCGCTCGAG ATGAGCGGGG GCGAGGAGCT 
     5221 GTTCGCCGGC ATCGTGCCCG TGCTGATCGA GCTGGACGGC GACGTGCACG GCCACAAGTT 
     5281 CAGCGTGCGC GGCGAGGGCG AGGGCGACGC CGACTACGGC AAGCTGGAGA TCAAGTTCAT 
     5341 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG GTGACCACCC TCTGCTACGG 
     5401 CATCCAGTGC TTCGCCCGCT ACCCCGAGCA CATGAAGATG AACGACTTCT TCAAGAGCGC 
     5461 CATGCCCGAG GGCTACATCC AGGAGCGCAC CATCCAGTTC CAGGACGACG GCAAGTACAA 
     5521 GACCCGCGGC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 
     5581 CAAGGACTTC AAGGAGGACG GCAACATCCT GGGCCACAAG CTGGAGTACA GCTTCAACAG 
     5641 CCACAACGTG TACATCCGCC CCGACAAGGC CAACAACGGC CTGGAGGCTA ACTTCAAGAC 
     5701 CCGCCACAAC ATCGAGGGCG GCGGCGTGCA GCTGGCCGAC CACTACCAGA CCAACGTGCC 
     5761 CCTGGGCGAC GGCCCCGTGC TGATCCCCAT CAACCACTAC CTGAGCACTC AGACCAAGAT 
     5821 CAGCAAGGAC CGCAACGAGG CCCGCGACCA CATGGTGCTC CTGGAGTCCT TCAGCGCCTG 
     5881 CTGCCACACC CACGGCATGG ACGAGCTGTA CAGGTCCGGA CTCAGAGTTT GGGTAGGAAG 
     5941 CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA ACCCTGGACC 
     6001 TATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT 
     6061 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA 
     6121 TCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA 
     6181 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA 
     6241 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG 
     6301 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA 
     6361 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG 
     6421 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT 
     6481 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC 
     6541 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG 
     6601 ATGTACACCC AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA 
     6661 ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG 
     6721 TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 
     6781 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 
     6841 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 
     6901 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 
     6961 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 
     7021 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 
     7081 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 
     7141 AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 
     7201 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 
     7261 CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT 
     7321 GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA 
     7381 ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG 
     7441 GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG 
     7501 GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC 
     7561 TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT 
     7621 GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC 
     7681 TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC 
     7741 CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG 
     7801 TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT 
     7861 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC 
     7921 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG 
     7981 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT 
     8041 AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 
     8101 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 
     8161 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 
     8221 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 
     8281 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 
     8341 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 
     8401 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 
     8461 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 
     8521 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 
     8581 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 
     8641 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 
     8701 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 
     8761 TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA 
     8821 TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA 
     8881 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT 
     8941 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA 
     9001 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA 
     9061 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG 
     9121 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA 
     9181 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG 
     9241 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA 
     9301 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG 
     9361 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT 
     9421 TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     9481 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     9541 TTCCCCGAAA AGTGCCACCT GAC
//