LOCUS       RC203747                8007 bp    DNA     circular  SYN 
DEFINITION  SIPA1 (Myc-DDK-tagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 1
ACCESSION   NM_153253
VERSION     NM_153253.28
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4164..4255 
                     /label="Myc-DDK"  
     misc_feature    4285..4870 
                     /label="PolyA signal"  
     misc_feature    4342..4361 
                     /label="Restriction Enzyme"  
     misc_feature    5146..5818 
                     /label="ColE1"  
     misc_feature    6248..7042 
                     /label="Kan/Neo"  
     misc_feature    7077..7379 
                     /label="SV40 ori"  
     misc_feature    7465..7942 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4146..4152 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4146 
                     /label="SIPA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCATGTGG GCCGGCGGTG TGGGGAGCCC TCGGCGGGGC ATGGCCCCTG 
     1081 CGTCCACAGA TGACCTCTTT GCCCGCAAGC TGCGCCAGCC AGCAAGGCCC CCGCTGACAC 
     1141 CGCACACCTT CGAGCCGAGG CCAGTCCGGG GCCCACTCCT GCGCAGCGGC AGCGATGCAG 
     1201 GCGAGGCCAG GCCCCCCACG CCAGCCAGCC CCCGTGCCCG TGCCCACAGC CACGAAGAGG 
     1261 CCAGCCGACC TGCAGCCACT TCCACCCGGC TCTTCACTGA CCCGCTGGCA CTGCTGGGGC 
     1321 TGCCAGCAGA GGAACCAGAG CCTGCCTTCC CACCAGTGCT TGAGCCTCGA TGGTTTGCCC 
     1381 ACTATGACGT GCAAAGCCTG CTCTTTGATT GGGCTCCGAG GTCTCAGGGG ATGGGGAGCC 
     1441 ACTCAGAGGC CAGCTCTGGG ACCCTGGCTT CAGCCGAGGA CCAGGCTGCC AGCTCGGACC 
     1501 TGCTGCATGG GGCACCTGGC TTTGTGTGTG AGCTCGGGGG TGAGGGTGAG CTAGGCCTGG 
     1561 GTGGACCAGC ATCCCCACCT GTGCCCCCTG CACTGCCCAA CGCGGCCGTG TCCATCCTGG 
     1621 AGGAGCCACA GAACCGAACC TCGGCCTACA GCCTGGAGCA CGCAGACCTG GGTGCTGGCT 
     1681 ACTACCGCAA ATACTTCTAT GGCAAAGAAC ATCAGAACTT CTTCGGGATG GACGAGTCGC 
     1741 TGGGCCCGGT GGCAGTGAGC CTGCGGCGGG AGGAGAAGGA GGGCAGCGGA GGGGGCACCC 
     1801 TGCACAGCTA CCGCGTCATC GTGCGGACCA CGCAGCTCCG GACACTCCGT GGCACCATCT 
     1861 CGGAGGACGC GCTGCCGCCG GGGCCCCCAC GGGGTCTGTC CCCAAGGAAA CTTCTGGAGC 
     1921 ACGTGGCGCC GCAGCTGAGC CCCAGCTGCT TGCGCCTGGG CTCAGCTTCA CCCAAGGTAC 
     1981 CACGGACGCT GCTCACACTG GATGAGCAAG TGCTGAGCTT CCAACGCAAG GTGGGCATCC 
     2041 TGTACTGCCG GGCGGGCCAG GGCTCGGAGG AGGAGATGTA CAACAACCAG GAGGCGGGAC 
     2101 CGGCCTTCAT GCAGTTTCTC ACCTTGCTGG GCGATGTGGT GCGGCTCAAA GGCTTTGAGA 
     2161 GTTACCGGGC CCAGCTAGAC ACCAAAACGG ATTCCACAGG CACGCACTCC CTCTACACCA 
     2221 CATACCAGGA CCACGAGATC ATGTTCCACG TGTCCACGAT GCTGCCTTAC ACCCCTAATA 
     2281 ACCAGCAGCA GCTCCTCCGG AAGCGCCACA TTGGCAACGA CATTGTGACC ATCGTGTTCC 
     2341 AGGAGCCTGG CAGCAAGCCC TTCTGCCCCA CCACCATCCG CTCGCACTTC CAGCACGTGT 
     2401 TCCTAGTGGT GCGGGCACAC ACACCCTGCA CGCCACACAC CACCTACAGG GTGGCCGTGA 
     2461 GCCGCACCCA GGACACCCCT GCCTTCGGGC CAGCTCTGCC TGCTGGCGGA GGCCCCTTCG 
     2521 CAGCCAACGC CGACTTCCGG GCCTTCCTGC TGGCCAAAGC GCTGAATGGT GAGCAGGCGG 
     2581 CCGGCCACGC GCGCCAGTTC CACGCCATGG CCACGCGCAC CCGCCAGCAG TACCTGCAAG 
     2641 ACCTGGCCAC CAACGAGGTG ACCACTACGT CGCTGGACTC GGCTTCACGC TTCGGCCTGC 
     2701 CCTCCCTGGG TGGGAGGCGC CGGGCGGCCC CTCGGGGCCC AGGCGCCGAG CTGCAGGCAG 
     2761 CGGGCTCACT GGTGTGGGGA GTGCGCGCGG CGCCCGGGGC GCGGGTCGCC GCCGGGGCTC 
     2821 AGGCGAGCGG CCCCGAAGGC ATCGAGGTGC CCTGCCTGCT GGGCATCTCG GCCGAGGCTC 
     2881 TGGTGCTGGT GGCGCCGCGC GACGGCCGCG TAGTGTTCAA TTGCGCCTGT CGCGACGTGC 
     2941 TGGCCTGGAC CTTCTCCGAG CAGCAGCTGG ACCTGTACCA CGGCCGCGGG GAGGCGATCA 
     3001 CGCTGCGCTT CGACGGGTCC CCCGGCCAAG CCGTGGGCGA GGTGGTGGCG CGCCTGCAGC 
     3061 TGGTGAGCCG TGGCTGCGAG ACCCGCGAGC TGGCGCTGCC CCGCGACGGT CAAGGCCGCC 
     3121 TGGGCTTCGA GGTGGACGCC GAGGGATTCG TCACGCACGT GGAGCGCTTC ACATTCGCCG 
     3181 AGACGGCGGG GCTGCGGCCC GGGGCGCGCC TCCTGCGCGT GTGCGGCCAG ACGCTGCCCA 
     3241 GCCTCCGGCC CGAGGCCGCT GCCCAGCTCC TGCGCTCGGC GCCCAAGGTC TGCGTCACCG 
     3301 TCCTGCCCCC CGACGAGAGC GGCCGGCCCC GCAGGAGTTT TTCGGAGCTG TACACGCTGT 
     3361 CGCTGCAGGA GCCTAGCCGG CGGGGGGCCC CAGATCCTGT GCAGGATGAG GTCCAGGGGG 
     3421 TGACCCTGCT GCCCACCACA AAGCAGCTGC TGCACCTGTG CCTGCAAGAT GGTGGCAGTC 
     3481 CTCCAGGGCC TGGGGATCTG GCCGAGGAGA GGACTGAGTT CCTGCACAGC CAGAACTCGC 
     3541 TGTCACCACG CAGCTCTCTG TCGGATGAGG CCCCAGTCCT GCCCAACACC ACCCCGGACC 
     3601 TCCTCCTGGC CACCACAGCC AAGCCATCAG TACCCAGTGC TGACAGTGAG ACACCCCTGA 
     3661 CCCAGGACAG GCCAGGCAGT CCCAGTGGCT CTGAGGACAA GGGCAACCCG GCGCCGGAGC 
     3721 TGAGGGCCTC CTTTCTGCCA CGTACCTTGT CTCTGCGGAA CTCCATCAGC AGGATCATGT 
     3781 CGGAGGCGGG CAGTGGGACC CTGGAGGACG AGTGGCAGGC CATCTCGGAG ATTGCCTCTA 
     3841 CTTGCAACAC CATTCTGGAG TCGCTGTCCC GAGAGGGACA GCCCATCCCA GAGAGTGGAG 
     3901 ACCCTAAGGG AACTCCAAAA TCTGATGCTG AGCCAGAGCC TGGGAACCTC TCAGAGAAGG 
     3961 TCTCTCACTT GGAGTCCATG CTCAGGAAGC TGCAGGAGGA CCTGCAGAAG GAGAAGGCGG 
     4021 ACAGGGCGGC CCTGGAGGAG GAGGTGCGGA GCCTGAGACA CAACAACCGG CGGCTGCAGG 
     4081 CGGAGTCTGA GAGTGCAGCC ACACGCCTCC TCCTGGCCTC CAAGCAGCTG GGCTCACCCA 
     4141 CCGCCGACCT GGCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4201 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4261 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4321 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4381 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4441 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4501 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4561 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4621 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4681 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4741 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4801 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4861 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4921 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4981 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5041 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5641 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5701 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5761 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5821 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5881 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5941 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6001 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6061 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6121 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6181 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6241 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6301 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6361 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6421 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6481 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6541 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6601 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6661 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6721 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6781 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6841 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6901 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6961 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7021 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7081 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7141 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7201 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7441 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7501 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7561 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7621 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7681 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7741 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7801 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7861 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7921 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7981 ATTTAACAAA AATTTAACGC GAATTTT
//