LOCUS       RC203733                8064 bp    DNA     circular  SYN 
DEFINITION  GEMIN4 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4)
ACCESSION   NM_015721
VERSION     NM_015721.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4219..4310 
                     /label="Myc-DDK"  
     misc_feature    4340..4925 
                     /label="PolyA signal"  
     misc_feature    4397..4416 
                     /label="Restriction Enzyme"  
     misc_feature    5201..5873 
                     /label="ColE1"  
     misc_feature    6303..7097 
                     /label="Kan/Neo"  
     misc_feature    7132..7434 
                     /label="SV40 ori"  
     misc_feature    7520..7997 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4194..4201 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4194 
                     /label="GEMIN4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCTAGGA CCCTTGAACA TCTGTGAAGA AATGACTATT CTGCATGGAG 
     1081 GCTTCTTGCT GGCCGAGCAG CTGTTCCACC CTAAGGCACT GGCAGAATTA ACAAAGTCTG 
     1141 ACTGGGAACG TGTTGGACGG CCCATCGTGG AGGCCTTAAG GGAGATCTCC TCGGCTGCAG 
     1201 CACACTCCCA GCCCTTTGCC TGGAAGAAGA AAGCCCTGAT CATCATCTGG GCCAAGGTTC 
     1261 TGCAGCCGCA CCCCGTGACC CCGTCCGACA CAGAGACACG GTGGCAGGAA GACCTGTTCT 
     1321 TCTCGGTGGG CAACATGATC CCCACCATCA ACCACACCAT CCTCTTCGAG CTGCTCAAAT 
     1381 CCCTGGAAGC TTCTGGACTC TTTATCCAGC TCCTGATGGC CCTGCCCACC ACCATCTGCC 
     1441 ATGCAGAACT AGAGCGCTTT CTGGAACATG TGACCGTTGA CACTTCTGCC GAAGACGTGG 
     1501 CCTTCTTCCT GGACGTCTGG TGGGAGGTGA TGAAGCACAA GGGTCACCCG CAGGACCCCC 
     1561 TGCTCTCCCA GTTTAGTGCA ATGGCCCATA AGTACCTGCC TGCCTTAGAT GAGTTCCCCC 
     1621 ATCCTCCAAA GAGGCTTAGG TCAGACCCAG ACGCGTGCCC CACCATGCCC CTGTTGGCCA 
     1681 TGCTGCTCCG CGGGCTGACA CAGATCCAGA GTCGGATCCT GGGCCCGGGG AGGAAGTGCT 
     1741 GTGCGCTGGC CAACCTGGCT GACATGCTGA CTGTGTTTGC GCTGACAGAG GACGACCCCC 
     1801 AGGAGGTGTC TGCAACCGTG TATCTGGACA AACTGGCCAC GGTGATCTCT GTGTGGAACT 
     1861 CGGACACCCA GAATCCCTAC CACCAGCAGG CGCTGGCAGA GAAGGTGAAG GAGGCAGAAC 
     1921 GGGATGTCAG CCTGACCTCG CTGGCCAAAC TCCCCAGTGA GACCATTTTC GTGGGCTGCG 
     1981 AGTTCCTGCA CCACCTGCTG CGGGAGTGGG GGGAGGAGTT GCAGGCCGTG CTCCGCAGCA 
     2041 GCCAGGGGAC AAGTTACGAC AGCTACCGGC TGTGCGACAG TCTGACTTCC TTCAGCCAGA 
     2101 ACGCGACGCT CTACCTGAAC CGCACCAGCC TGTCCAAGGA GGACAGGCAG GTGGTCTCTG 
     2161 AGCTGGCGGA GTGTGTCAGG GACTTCCTGA GGAAAACGAG CACGGTGCTG AAGAACAGGG 
     2221 CCTTGGAGGA TATCACAGCT TCCATTGCCA TGGCCGTCAT CCAGCAGAAG ATGGACCGCC 
     2281 ATATGGAAGT GTGCTACATT TTTGCCTCTG AGAAGAAGTG GGCCTTCTCG GACGAGTGGG 
     2341 TAGCCTGCCT GGGGAGTAAC AGGGCCCTCT TCCGACAGCC AGACTTGGTG TTGAGGCTGC 
     2401 TGGAAACAGT GATAGACGTC AGCACAGCTG ACAGAGCCAT CCCTGAGTCT CAGATCCGGC 
     2461 AGGTGATCCA CCTGATCCTG GAATGTTACG CAGACCTCTC CCTGCCAGGT AAAAATAAAG 
     2521 TCCTTGCAGG TATCCTGCGT TCCTGGGGGC GAAAGGGCCT CTCTGAAAAG TTGCTGGCTT 
     2581 ATGTGGAGGG TTTTCAGGAA GACCTCAATA CAACTTTTAA CCAGCTCACT CAGAGTGCCT 
     2641 CCGAACAGGG CTTGGCAAAA GCTGTGGCCT CCGTGGCCCG CCTGGTCATA GTGCACCCGG 
     2701 AAGTCACGGT GAAGAAAATG TGCAGCCTGG CTGTGGTCAA TCTCGGCACC CACAAGTTCC 
     2761 TGGCCCAGAT TCTCACTGCC TTCCCTGCCC TTAGGTTTGT GGAAGAGCAG GGTCCCAATT 
     2821 CATCTGCCAC TTTCATGGTG TCATGCCTCA AAGAAACCGT CTGGATGAAG TTCTCTACAC 
     2881 CCAAGGAAGA AAAGCAATTT TTAGAGCTCC TGAACTGCCT GATGAGTCCC GTGAAACCCC 
     2941 AAGGGATTCC AGTGGCTGCT CTTCTTGAGC CAGACGAGGT GCTGAAGGAA TTTGTCCTGC 
     3001 CTTTCTTGAG GTTAGATGTT GAAGAGGTAG ACCTCAGTCT GAGGATCTTC ATCCAGACTC 
     3061 TAGAGGCAAA CGCGTGCCGA GAGGAATACT GGCTCCAGAC CTGCTCCCCG TTTCCACTCC 
     3121 TCTTCAGCTT GTGCCAGCTC TTGGACCGCT TCAGCAAATA CTGGCAGCTT CCCAAGGAGA 
     3181 AGCGGTGCCT CTCTTTGGAT AGGAAGGATC TAGCGATCCA TATCCTGGAG CTCCTGTGTG 
     3241 AGATTGTATC AGCCAATGCT GAGACCTTCT CCCCGGATGT CTGGATCAAG TCCCTGTCCT 
     3301 GGCTCCACCG CAAGTTAGAA CAGCTAGACT GGACTGTGGG CCTGAGGCTG AAGAGCTTCT 
     3361 TCGAGGGGCA CTTCAAGTGT GAAGTGCCAG CCACACTTTT TGAGATCTGT AAGCTTTCAG 
     3421 AAGACGAGTG GACCTCCCAG GCCCACCCAG GGTACGGGGC TGGCACGGGG CTCCTGGCCT 
     3481 GGATGGAGTG CTGCTGCGTC TCCAGCGGCA TCTCGGAGAG GATGCTGTCT CTCTTGGTGG 
     3541 TGGACGTGGG CAATCCTGAG GAGGTCAGAC TGTTCAGCAA AGGCTTTCTG GTGGCCCTGG 
     3601 TGCAAGTCAT GCCTTGGTGC AGCCCTCAGG AGTGGCAGCG CCTTCACCAG CTGACCAGGA 
     3661 GACTGCTGGA GAAGCAGCTC CTCCATGTCC CTTATAGCCT GGAATATATT CAGTTTGTTC 
     3721 CCCTGCTCAA CCTGAAGCCC TTTGCCCAGG AGTTGCAACT CTCCGTCCTC TTCCTGAGGA 
     3781 CTTTCCAGTT TCTCTGCAGC CATAGCTGTC GTGATTGGCT TCCTCTGGAA GGCTGGAACC 
     3841 ACGTGGTCAA ACTCCTCTGT GGCAGTCTGA CCCGCCTCCT GGACTCAGTC AGGGCGATAC 
     3901 AGGCAGCTGG CCCTTGGGTT CAAGGACCAG AGCAGGACCT GACCCAGGAA GCCCTGTTTG 
     3961 TTTACACCCA GGTGTTCTGC CATGCTCTGC ACATCATGGC CATGCTCCAC CCGGAGGTCT 
     4021 GTGAGCCACT CTACGTTTTA GCCTTGGAAA CCCTCACCTG CTATGAGACT TTGAGCAAGA 
     4081 CCAACCCTTC TGTCAGCTCC TTGCTCCAGA GGGCACACGA GCAGCGCTTC TTAAAGTCCA 
     4141 TTGCTGAGGG CATCGGCCCT GAAGAACGGC GCCAAACCCT GTTGCAGAAG ATGAGCAGCT 
     4201 TCAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4261 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4321 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4381 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4441 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4501 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4561 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4621 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4681 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4741 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4801 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4861 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4921 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4981 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5041 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5101 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5161 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5221 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5281 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5341 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5401 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5461 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5521 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5581 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5641 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5701 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5761 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5821 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5881 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5941 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6001 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6061 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6121 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6181 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6241 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6301 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6361 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6421 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6481 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6541 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6601 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6661 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6721 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6781 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6841 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6901 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6961 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7021 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7081 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7141 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7201 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7261 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7321 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7381 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7501 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7561 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7621 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7681 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7741 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7801 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7861 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7921 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7981 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8041 TAACAAAAAT TTAACGCGAA TTTT
//