LOCUS       RC203647                8751 bp    DNA     circular  SYN 
DEFINITION  SETDB1 (Myc-DDK-tagged)-Human SET domain, bifurcated 1 (SETDB1), transcript variant 2
ACCESSION   NM_012432
VERSION     NM_012432.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4908..4999 
                     /label="Myc-DDK"  
     misc_feature    5029..5614 
                     /label="PolyA signal"  
     misc_feature    5086..5105 
                     /label="Restriction Enzyme"  
     misc_feature    5890..6562 
                     /label="ColE1"  
     misc_feature    6992..7786 
                     /label="Kan/Neo"  
     misc_feature    7821..8123 
                     /label="SV40 ori"  
     misc_feature    8209..8686 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4890..4896 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4890 
                     /label="SETDB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTCCCTT CCTGGGTGCA TTGGTTTGGA TGCAGCAACA GCTACAGTGG 
     1081 AGTCTGAAGA GATTGCAGAG CTGCAACAGG CAGTGGTTGA GGAACTGGGT ATCTCTATGG 
     1141 AGGAACTTCG GCATTTCATC GATGAGGAAC TGGAGAAGAT GGATTGTGTA CAGCAACGCA 
     1201 AGAAGCAGCT AGCAGAGTTA GAGACATGGG TAATACAGAA AGAATCTGAG GTGGCTCACG 
     1261 TTGACCAACT CTTTGATGAT GCATCCAGGG CAGTGACTAA TTGTGAGTCT TTGGTGAAGG 
     1321 ACTTCTACTC CAAGCTGGGA CTACAATACC GGGACAGTAG CTCTGAGGAC GAATCTTCCC 
     1381 GGCCTACAGA AATAATTGAG ATTCCTGATG AAGATGATGA TGTCCTCAGT ATTGATTCAG 
     1441 GTGATGCTGG GAGCAGAACT CCAAAAGACC AGAAGCTCCG TGAAGCTATG GCTGCCTTAA 
     1501 GAAAGTCAGC TCAAGATGTT CAGAAGTTCA TGGATGCTGT CAACAAGAAG AGCAGTTCCC 
     1561 AGGATCTGCA TAAAGGAACC TTGAGTCAGA TGTCTGGAGA ACTAAGCAAA GATGGTGACC 
     1621 TGATAGTCAG CATGCGAATT CTGGGCAAGA AGAGAACTAA GACTTGGCAC AAAGGCACCC 
     1681 TTATTGCCAT CCAGACAGTT GGGCCAGGGA AGAAATACAA GGTGAAATTT GACAACAAAG 
     1741 GAAAGAGTCT ACTGTCGGGG AACCATATTG CCTATGATTA CCACCCTCCT GCTGACAAGC 
     1801 TGTATGTGGG CAGTCGGGTG GTCGCCAAAT ACAAAGATGG GAATCAGGTC TGGCTCTATG 
     1861 CTGGCATTGT AGCTGAGACA CCAAACGTCA AAAACAAGCT CAGGTTTCTC ATTTTCTTTG 
     1921 ATGATGGCTA TGCTTCCTAT GTCACACAGT CGGAACTGTA TCCCATTTGC CGGCCACTGA 
     1981 AAAAGACTTG GGAGGACATA GAAGACATCT CCTGCCGTGA CTTCATAGAG GAGTATGTCA 
     2041 CTGCCTACCC CAACCGCCCC ATGGTACTGC TCAAGAGTGG CCAGCTTATC AAGACTGAGT 
     2101 GGGAAGGCAC GTGGTGGAAG TCCCGAGTTG AGGAGGTGGA TGGCAGCCTA GTCAGGATCC 
     2161 TCTTCCTGGA TGACAAAAGA TGTGAGTGGA TCTATCGAGG CTCTACACGG CTGGAGCCCA 
     2221 TGTTCAGCAT GAAAACATCC TCAGCCTCTG CACTGGAGAA GAAGCAAGGA CAGCTCAGGA 
     2281 CACGTCCAAA TATGGGTGCT GTGAGGAGCA AAGGCCCTGT TGTCCAGTAC ACACAGGATC 
     2341 TGACCGGTAC TGGAACCCAG TTCAAGCCAG TGGAACCCCC ACAGCCTACA GCTCCACCTG 
     2401 CCCCACCTTT CCCACCTGCT CCACCTCTAT CCCCCCAAGC AGGTGACAGT GACTTGGAAA 
     2461 GCCAGCTTGC CCAGTCACGG AAGCAGGTAG CCAAAAAGAG CACGTCCTTT CGACCAGGAT 
     2521 CTGTGGGCTC TGGTCATTCC TCCTCTACAT CTCCTGCACT CAGTGAAAAT GTCTCTGGTG 
     2581 GGAAACCTGG GATCAACCAG ACATATAGAT CACCTTTAGG CTCCACAGCC TCTGCCCCAG 
     2641 CACCCTCAGC ACTCCCGGCC CCTCCAGCAC CCCCAGTCTT CCATGGCATG CTGGAGCGGG 
     2701 CCCCAGCAGA GCCCTCCTAC CGTGCTCCCA TGGAGAAGCT TTTCTACTTA CCTCATGTCT 
     2761 GCAGCTATAC CTGCCTGTCT CGAGTCAGAC CTATGAGGAA TGAGCAGTAC CGGGGCAAGA 
     2821 ACCCTCTGCT GGTCCCGTTA CTATATGACT TCCGGCGGAT GACAGCCCGG CGTCGAGTTA 
     2881 ACCGCAAGAT GGGCTTTCAT GTTATCTATA AGACACCTTG TGGTCTCTGC CTTCGGACAA 
     2941 TGCAGGAGAT AGAACGCTAC CTTTTCGAGA CTGGCTGTGA CTTCCTCTTC CTGGAGATGT 
     3001 TCTGTTTGGA TCCATATGTT CTTGTGGACC GAAAGTTTCA GCCCTATAAG CCTTTTTACT 
     3061 ATATTTTGGA CATCACTTAT GGGAAGGAAG ATGTTCCCCT ATCCTGTGTC AATGAGATTG 
     3121 ACACAACCCC TCCACCCCAG GTGGCCTACA GCAAGGAACG TATCCCGGGC AAGGGTGTTT 
     3181 TCATTAACAC AGGCCCTGAA TTTCTGGTTG GCTGTGACTG CAAGGATGGG TGTCGGGACA 
     3241 AGTCCAAGTG TGCCTGCCAT CAACTAACTA TCCAGGCTAC AGCCTGTACC CCAGGAGGCC 
     3301 AAATCAACCC TAACTCTGGC TACCAGTACA AGAGACTAGA AGAGTGTCTA CCCACAGGGG 
     3361 TATATGAGTG TAACAAACGC TGCAAATGTG ACCCAAACAT GTGCACAAAC CGGTTGGTGC 
     3421 AACATGGACT ACAAGTTCGG CTACAGCTAT TCAAGACACA GAACAAGGGC TGGGGTATCC 
     3481 GCTGCTTGGA TGACATTGCC AAAGGCTCTT TTGTTTGTAT TTATGCAGGC AAAATCCTGA 
     3541 CAGATGACTT TGCAGACAAG GAGGGTCTGG AAATGGGTGA TGAGTACTTT GCAAATCTGG 
     3601 ACCATATCGA GAGCGTGGAG AACTTCAAAG AAGGATATGA GAGTGATGCC CCCTGTTCCT 
     3661 CTGACAGCAG TGGTGTAGAC TTGAAGGACC AGGAAGATGG CAACAGCGGT ACAGAGGACC 
     3721 CTGAAGAGTC CAATGATGAT AGCTCAGATG ATAACTTCTG TAAGGATGAG GACTTCAGCA 
     3781 CCAGTTCAGT GTGGCGGAGC TATGCTACCC GGAGGCAGAC CCGGGGCCAG AAAGAGAACG 
     3841 GACTCTCTGA GACAACTTCC AAGGACTCCC ACCCCCCAGA TCTTGGACCC CCACATATTC 
     3901 CTGTTCCTCC CTCAATCCCT GTAGGTGGCT GCAATCCACC TTCCTCCGAA GAGACACCCA 
     3961 AGAACAAGGT GGCCTCATGG TTGAGCTGCA ATAGTGTCAG TGAAGGTGGT TTTGCTGACT 
     4021 CTGATAGCCA TTCATCCTTC AAGACTAATG AAGGTGGGGA GGGCCGGGCT GGGGGAAGCC 
     4081 GAATGGAGGC TGAGAAGGCC TCCACCTCAG GACTAGGCAT CAAGGATGAG GGAGACATCA 
     4141 AACAGGCCAA GAAAGAGGAC ACTGACGACC GAAACAAGAT GTCAGTAGTT ACTGAAAGCT 
     4201 CTCGAAATTA CGGTTACAAT CCTTCTCCTG TGAAGCCTGA AGGACTTCGC CGCCCACCTA 
     4261 GTAAGACTAG TATGCATCAA AGCCGAAGAC TCATGGCTTC TGCTCAGTCC AACCCTGATG 
     4321 ATGTCCTGAC ACTGTCCAGC AGCACAGAAA GTGAGGGGGA AAGTGGGACC AGCCGAAAGC 
     4381 CCACTGCTGG TCAGACTTCG GCTACAGCGG TTGACAGTGA TGATATCCAG ACCATATCCT 
     4441 CTGGCTCTGA AGGGGATGAC TTTGAGGACA AGAAGAACAT GACTGGTCCA ATGAAGCGTC 
     4501 AAGTGGCAGT AAAATCAACC CGAGGCTTTG CTCTTAAATC AACCCATGGG ATTGCAATTA 
     4561 AATCAACCAA CATGGCCTCT GTGGACAAGG GGGAGAGCGC ACCTGTTCGT AAGAACACAC 
     4621 GCCAATTCTA TGATGGCGAG GAGTCTTGCT ACATCATTGA TGCCAAGCTT GAAGGCAACC 
     4681 TGGGCCGCTA CCTCAACCAC AGTTGCAGCC CCAACCTGTT TGTCCAGAAT GTCTTCGTGG 
     4741 ATACCCATGA TCTTCGCTTC CCCTGGGTGG CCTTCTTTGC CAGCAAAATC CGGGCTGGGA 
     4801 CAGAACTTAC TTGGGACTAC AACTACGAGG TGGGCAGTGT GGAAGGCAAG GAGCTACTCT 
     4861 GTTGCTGTGG GGCCATTGAA TGCAGAGGAC GTCTTCTTAC GCGTACGCGG CCGCTCGAGC 
     4921 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4981 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5041 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5101 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5161 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5221 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5281 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5341 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5401 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5461 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5521 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5581 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5641 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5701 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5761 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5821 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5881 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5941 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6001 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6061 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6121 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6181 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6241 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6301 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6361 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6421 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6481 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6541 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6601 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6661 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6721 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6781 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6841 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6901 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6961 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7021 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7081 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7141 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7201 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7261 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7321 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7381 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7441 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7501 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7561 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7621 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7681 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7741 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7801 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7861 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7921 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7981 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8041 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8161 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8221 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8281 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8341 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8401 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8461 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8521 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8581 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8641 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8701 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//