LOCUS       RC203519                9210 bp    DNA     circular  SYN 
DEFINITION  CPSF1 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1)
ACCESSION   NM_013291
VERSION     NM_013291.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5367..5458 
                     /label="Myc-DDK"  
     misc_feature    5488..6073 
                     /label="PolyA signal"  
     misc_feature    5545..5564 
                     /label="Restriction Enzyme"  
     misc_feature    6349..7021 
                     /label="ColE1"  
     misc_feature    7451..8245 
                     /label="Kan/Neo"  
     misc_feature    8280..8582 
                     /label="SV40 ori"  
     misc_feature    8668..9145 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5349..5355 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5349 
                     /label="CPSF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACGCCGTG TACAAACAGG CGCATCCGCC CACCGGTCTG GAGTTCTCCA 
     1081 TGTACTGCAA CTTCTTCAAC AACAGCGAGC GCAACCTGGT AGTGGCCGGG ACCTCGCAGC 
     1141 TCTACGTGTA CCGCCTCAAC CGCGACGCCG AGGCTCTGAC CAAGAATGAC AGGAGCACAG 
     1201 AGGGGAAGGC CCACCGGGAG AAGCTCGAGC TTGCTGCCTC CTTCTCCTTC TTTGGCAACG 
     1261 TCATGTCCAT GGCCAGCGTG CAGCTGGCAG GAGCCAAGCG GGATGCCCTG CTCCTAAGCT 
     1321 TCAAGGATGC CAAGCTGTCT GTGGTGGAGT ACGACCCGGG CACCCATGAC CTGAAGACCC 
     1381 TGTCACTGCA CTACTTTGAG GAGCCTGAGC TTCGGGACGG GTTTGTGCAG AATGTACACA 
     1441 CGCCGCGAGT GCGGGTGGAC CCCGACGGGC GCTGTGCAGC CATGCTTGTC TACGGCACGC 
     1501 GGCTGGTGGT CCTGCCCTTC CGCAGGGAGA GCCTGGCTGA GGAGCACGAG GGGCTCGTGG 
     1561 GTGAGGGGCA GAGGTCCAGC TTCCTGCCCA GCTACATCAT CGACGTGCGG GCCCTAGACG 
     1621 AGAAGCTGCT CAACATCATC GACCTGCAGT TCCTGCATGG CTACTACGAG CCTACCCTCC 
     1681 TCATCCTGTT TGAGCCCAAC CAGACCTGGC CTGGGCGCGT GGCCGTGCGG CAGGACACGT 
     1741 GCTCCATTGT GGCCATCTCA CTGAACATCA CGCAGAAGGT GCACCCCGTC ATCTGGTCCC 
     1801 TCACCAGCCT GCCCTTTGAC TGCACCCAGG CTCTGGCTGT GCCCAAGCCC ATAGGTGGGG 
     1861 TGGTGGTGTT TGCCGTCAAC TCGCTGTTGT ACCTGAACCA GAGCGTCCCC CCGTATGGCG 
     1921 TGGCTCTCAA CAGCCTCACC ACAGGAACCA CGGCTTTCCC GCTTCGCACC CAGGAGGGTG 
     1981 TGCGGATCAC CCTGGACTGC GCCCAGGCCA CCTTCATCTC CTACGACAAG ATGGTCATCT 
     2041 CCCTCAAGGG CGGCGAGATC TACGTGCTGA CCCTCATCAC CGACGGCATG CGCAGTGTCC 
     2101 GAGCGTTCCA CTTTGACAAG GCGGCCGCCA GCGTCCTCAC CACCAGCATG GTCACCATGG 
     2161 AGCCCGGGTA CCTGTTCCTG GGTTCTCGCC TGGGCAATTC CCTCCTCCTC AAGTACACGG 
     2221 AGAAGCTGCA GGAGCCCCCG GCCAGTGCTG TCCGTGAGGC TGCCGACAAG GAAGAGCCTC 
     2281 CCTCAAAGAA GAAGCGAGTG GATGCGACGG CCGGCTGGTC AGCTGCGGGT AAGTCGGTGC 
     2341 CGCAGGATGA GGTGGACGAG ATTGAAGTGT ACGGCAGCGA GGCCCAGTCG GGAACACAGC 
     2401 TGGCCACCTA CTCCTTTGAG GTGTGTGACA GCATCCTGAA CATTGGACCC TGTGCCAATG 
     2461 CCGCCGTGGG CGAGCCTGCC TTCCTCTCTG AAGAGTTTCA GAACAGCCCC GAGCCGGACC 
     2521 TGGAGATTGT GGTTTGCTCC GGCCACGGGA AGAACGGGGC TTTGTCGGTG CTGCAGAAGA 
     2581 GCATCCGGCC CCAGGTGGTG ACAACCTTTG AGCTTCCCGG CTGCTATGAC ATGTGGACAG 
     2641 TCATCGCCCC GGTGCGTAAG GAGGAGGAGG ACAATCCCAA GGGGGAGGGC ACAGAGCAGG 
     2701 AACCCAGCAC CACCCCTGAA GCAGACGACG ACGGCCGCAG ACACGGATTC CTGATTCTGA 
     2761 GCCGGGAAGA CTCCACCATG ATCCTGCAGA CGGGGCAGGA GATCATGGAG CTGGACACCA 
     2821 GTGGCTTCGC CACTCAGGGC CCCACGGTCT TTGCTGGGAA CATCGGGGAC AACCGCTACA 
     2881 TTGTCCAAGT GTCACCACTG GGCATCCGCC TGCTGGAAGG AGTGAATCAG CTGCACTTCA 
     2941 TCCCCGTGGA CCTGGGCGCC CCCATCGTGC AGTGCGCCGT GGCCGACCCC TATGTGGTCA 
     3001 TCATGAGTGC CGAGGGCCAC GTCACCATGT TCCTGCTGAA GAGTGACTCC TACGGTGGCC 
     3061 GCCACCACCG CCTGGCGCTG CACAAGCCCC CGCTGCACCA TCAGTCCAAG GTGATTACGC 
     3121 TGTGCCTGTA CCGAGACCTC AGCGGCATGT TCACCACTGA GAGCCGCCTG GGTGGGGCCC 
     3181 GTGACGAGCT CGGGGGCCGC AGTGGCCCGG AGGCCGAGGG CCTGGGCTCA GAGACTAGCC 
     3241 CCACAGTGGA TGACGAGGAG GAGATGCTGT ATGGGGATTC GGGCTCCCTC TTCAGCCCCA 
     3301 GCAAGGAGGA GGCCCGAAGA AGCAGCCAGC CCCCTGCTGA CCGGGACCCT GCACCCTTCC 
     3361 GGGCAGAGCC TACCCACTGG TGCCTGCTGG TGCGGGAGAA TGGCACCATG GAGATCTACC 
     3421 AGCTTCCCGA CTGGCGGCTG GTGTTCCTGG TGAAGAACTT CCCTGTGGGG CAGCGGGTCC 
     3481 TTGTGGACAG CTCCTTTGGA CAGCCCACTA CACAGGGCGA GGCCCGCAGG GAGGAGGCCA 
     3541 CGCGCCAGGG GGAGCTGCCC CTCGTCAAGG AGGTGCTGCT GGTGGCGCTG GGCAGCCGCC 
     3601 AGAGCAGGCC CTACCTGCTG GTGCATGTGG ACCAAGAGCT GCTTATCTAC GAGGCCTTCC 
     3661 CCCACGACTC TCAGCTCGGC CAGGGCAATC TCAAAGTCCG CTTTAAGAAG GTCCCTCACA 
     3721 ACATCAACTT CCGTGAGAAG AAGCCAAAGC CATCCAAGAA GAAAGCAGAA GGTGGCGGCG 
     3781 CAGAGGAGGG GGCTGGGGCC CGGGGCCGCG TGGCGCGTTT CCGCTACTTC GAGGATATTT 
     3841 ATGGCTACTC AGGGGTCTTC ATCTGCGGCC CCTCCCCTCA CTGGCTCTTG GTGACCGGCC 
     3901 GAGGGGCTCT GCGGCTACAC CCCATGGCCA TCGACGGCCC GGTCGACTCT TTCGCTCCAT 
     3961 TCCACAATGT CAACTGTCCC CGCGGCTTCC TGTACTTCAA CAGACAGGGC GAGCTGAGGA 
     4021 TCAGTGTCCT GCCTGCCTAC CTGTCCTATG ATGCCCCATG GCCTGTCAGG AAGATCCCGC 
     4081 TGCGCTGCAC GGCCCACTAT GTGGCTTACC ACGTGGAGTC TAAGGTGTAT GCTGTGGCCA 
     4141 CCAGCACCAA CACGCCGTGT GCCCGCATCC CACGCATGAC TGGCGAGGAG AAGGAGTTTG 
     4201 AGACCATCGA GAGAGATGAG CGGTACATCC ACCCCCAGCA GGAGGCCTTC TCCATCCAGC 
     4261 TCATCTCCCC GGTCAGCTGG GAGGCTATTC CCAATGCCAG GATCGAGCTG CAGGAGTGGG 
     4321 AGCATGTGAC CTGCATGAAG ACAGTGTCTC TGCGCAGTGA GGAGACCGTG TCGGGCCTCA 
     4381 AAGGCTACGT GGCCGCCGGG ACCTGCCTCA TGCAGGGGGA GGAGGTCACG TGCCGAGGGC 
     4441 GGATCTTGAT CATGGATGTG ATTGAGGTGG TGCCCGAGCC TGGCCAGCCC TTGACCAAGA 
     4501 ACAAGTTCAA AGTCCTTTAC GAGAAGGAGC AGAAGGGGCC CGTGACCGCC CTGTGCCACT 
     4561 GCAATGGCCA CCTGGTGTCG GCCATCGGCC AGAAGATTTT CCTGTGGAGC CTGCGGGCCA 
     4621 GCGAGCTGAC GGGCATGGCC TTCATCGACA CGCAGCTCTA CATACACCAG ATGATCAGCG 
     4681 TCAAGAACTT CATCCTGGCA GCCGACGTCA TGAAGAGCAT TTCGCTGCTG CGCTACCAGG 
     4741 AGGAAAGCAA GACGCTGAGC CTGGTGTCGC GGGATGCCAA GCCCCTGGAG GTGTACAGCG 
     4801 TGGACTTCAT GGTGGACAAT GCCCAGCTGG GTTTTCTGGT GTCTGACCGC GACCGCAACC 
     4861 TCATGGTGTA CATGTACCTG CCCGAAGCCA AGGAGAGTTT CGGGGGCATG CGCCTGCTGC 
     4921 GTCGGGCAGA CTTCCACGTG GGTGCCCACG TGAACACGTT CTGGAGGACC CCGTGCCGGG 
     4981 GGGCCACTGA AGGGCTCAGC AAAAAGTCGG TCGTGTGGGA GAATAAGCAC ATCACGTGGT 
     5041 TTGCCACCCT GGACGGCGGC ATCGGGCTGC TGCTGCCCAT GCAGGAGAAG ACCTACCGGC 
     5101 GGCTGCTGAT GCTGCAGAAC GCGCTGACCA CCATGCTGCC ACACCACGCC GGCCTCAACC 
     5161 CCCGCGCCTT CCGGATGCTG CACGTGGACC GCCGCACCCT CCAGAATGCC GTGCGCAACG 
     5221 TGCTGGATGG GGAGCTGCTC AACCGCTACC TGTACCTGAG CACCATGGAG CGCAGCGAGC 
     5281 TAGCCAAGAA GATCGGCACC ACACCAGACA TAATCCTGGA CGACTTGCTG GAGACGGACC 
     5341 GCGTCACCGC CCACTTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5401 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5461 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5521 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5581 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5641 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5701 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5761 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5821 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5881 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5941 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6001 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6061 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6121 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6181 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6241 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6301 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6361 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6421 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6481 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6541 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6601 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6661 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6721 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6781 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6841 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6901 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6961 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7021 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7081 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7141 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7201 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7261 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7321 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7381 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7441 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7501 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7561 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7621 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7681 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7741 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7801 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7861 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7921 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7981 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8041 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8101 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8161 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8221 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8281 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8341 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8401 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8461 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8581 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8641 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8761 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8821 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8881 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8941 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9001 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9061 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9121 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9181 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//