LOCUS       RC203364L2                9093 bp    DNA     circular  SYN 
DEFINITION  ACSS3 (Myc-DDK-tagged)-Human acyl-CoA synthetase short-chain family member 3 (ACSS3)
ACCESSION   NM_024560
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7384..7564 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8292..8951 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7612..8231 
                     /label="ColE1"  
     misc_feature    6259..6279 
                     /label="LR50"  
     misc_feature    5399..6124 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6268..6859 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5378..5384 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5378 
                     /label="ACSS3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAACCGTCT TGGCTGCAGT GTCGTAAAGT 
     3361 CACCAGCGCC GGGGGGCTCG GAGGGCCCTT GCCTGGGTCC TCTCCGGCCC GGGGAGCCGG 
     3421 TGCGGCCCTC AGGGCTTTAG TGGTCCCGGG CCCGCGGGGC GGTCTCGGGG GCCGGGGATG 
     3481 CAGGGCACTG TCCTCCGGCA GTGGCAGCGA GTACAAGACC CACTTCGCAG CCTCGGTGAC 
     3541 CGACCCCGAG AGGTTCTGGG GCAAAGCTGC CGAGCAGATC AGCTGGTACA AGCCCTGGAC 
     3601 CAAAACGCTG GAGAACAAAC ACTCGCCCTC TACCAGGTGG TTTGTGGAAG GAATGCTTAA 
     3661 CATTTGTTAC AATGCCGTTG ATCGTCATAT TGAAAATGGT AAAGGGGATA AGATTGCTAT 
     3721 CATCTATGAC AGTCCTGTTA CAAACACTAA AGCAACCTTT ACCTATAAAG AAGTTCTGGA 
     3781 GCAGGTCTCC AAGCTGGCTG GTGTCTTGGT CAAGCATGGC ATCAAGAAAG GTGACACTGT 
     3841 GGTTATCTAC ATGCCTATGA TCCCACAGGC GATGTATACC ATGTTGGCAT GTGCAAGGAT 
     3901 AGGTGCCATC CACAGTCTCA TATTTGGAGG ATTTGCTTCC AAAGAACTAA GTAGTCGCAT 
     3961 TGATCATGTA AAGCCCAAGG TGGTTGTTAC AGCATCATTT GGCATTGAAC CTGGAAGGAG 
     4021 GGTAGAGTAC GTACCACTTG TAGAAGAAGC GCTAAAAATA GGACAACACA AACCAGACAA 
     4081 AATTCTCATT TATAATCGTC CAAATATGGA GGCGGTTCCT TTGGCTCCCG GTCGTGACCT 
     4141 TGATTGGGAT GAAGAGATGG CAAAAGCCCA GTCACATGAC TGTGTTCCTG TTCTTTCAGA 
     4201 ACACCCACTG TATATTCTTT ACACATCTGG CACAACGGGG TTACCTAAGG GTGTGATTAG 
     4261 GCCCACTGGG GGATACGCTG TCATGCTACA CTGGTCAATG TCTTCCATAT ACGGACTTCA 
     4321 ACCCGGAGAG GTGTGGTGGG CAGCTTCTGA CTTAGGCTGG GTTGTTGGAC ATTCCTATAT 
     4381 CTGCTATGGA CCTCTTCTTC ATGGGAACAC AACAGTTTTA TATGAGGGGA AGCCTGTGGG 
     4441 AACACCAGAT GCTGGCGCTT ATTTCCGTGT GCTTGCAGAG CATGGAGTAG CTGCCTTGTT 
     4501 TACAGCACCA ACTGCAATTA GAGCAATCCG TCAACAGGAC CCTGGGGCAG CTTTGGGGAA 
     4561 GCAGTACTCT CTGACAAGGT TCAAAACATT ATTTGTGGCT GGAGAACGAT GTGATGTAGA 
     4621 GACCCTGGAA TGGTCCAAAA ATGTCTTCAG AGTACCTGTC TTAGACCATT GGTGGCAAAC 
     4681 TGAGACTGGA TCTCCAATTA CTGCGTCATG TGTTGGATTA GGCAATTCTA AAACACCTCC 
     4741 ACCAGGGCAA GCAGGAAAAA GCGTCCCAGG ATACAATGTT ATGATTTTGG ATGACAACAT 
     4801 GCAAAAACTG AAGGCTCGGT GTTTAGGAAA TATTGTGGTA AAGTTACCAT TGCCACCTGG 
     4861 GGCTTTTTCA GGACTCTGGA AGAATCAGGA AGCATTCAAG CATTTATACT TTGAAAAATT 
     4921 TCCTGGATAT TATGATACCA TGGATGCTGG TTACATGGAT GAAGAAGGCT ATTTGTATGT 
     4981 TATGTCTCGA GTGGATGATG TAATAAATGT TGCAGGTCAC AGAATTTCTG CAGGCGCCAT 
     5041 TGAAGAGTCA ATCCTTTCCC ATGGTACCGT GGCAGACTGT GCTGTTGTTG GCAAGGAAGA 
     5101 TCCCTTAAAA GGTCATGTCC CCTTAGCACT CTGTGTATTG AGAAAAGATA TAAATGCAAC 
     5161 AGAGGAGCAA GTTTTGGAAG AAATTGTGAA ACACGTTAGA CAGAACATTG GCCCTGTGGC 
     5221 TGCTTTTCGA AATGCAGTGT TTGTCAAACA GCTACCCAAA ACCAGATCTG GCAAGATCCC 
     5281 CCGATCAGCT TTATCTGCCA TTGTCAATGG CAAGCCATAC AAGATAACTT CTACAATTGA 
     5341 AGACCCCAGC ATTTTTGGCC ACGTAGAAGA AATGCTGAAG CAAGCAACGC GTACGCGGCC 
     5401 GCTCGAGATG AGCGGGGGCG AGGAGCTGTT CGCCGGCATC GTGCCCGTGC TGATCGAGCT 
     5461 GGACGGCGAC GTGCACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGCGAGG GCGACGCCGA 
     5521 CTACGGCAAG CTGGAGATCA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 
     5581 CACCCTGGTG ACCACCCTCT GCTACGGCAT CCAGTGCTTC GCCCGCTACC CCGAGCACAT 
     5641 GAAGATGAAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACATCCAGG AGCGCACCAT 
     5701 CCAGTTCCAG GACGACGGCA AGTACAAGAC CCGCGGCGAG GTGAAGTTCG AGGGCGACAC 
     5761 CCTGGTGAAC CGCATCGAGC TGAAGGGCAA GGACTTCAAG GAGGACGGCA ACATCCTGGG 
     5821 CCACAAGCTG GAGTACAGCT TCAACAGCCA CAACGTGTAC ATCCGCCCCG ACAAGGCCAA 
     5881 CAACGGCCTG GAGGCTAACT TCAAGACCCG CCACAACATC GAGGGCGGCG GCGTGCAGCT 
     5941 GGCCGACCAC TACCAGACCA ACGTGCCCCT GGGCGACGGC CCCGTGCTGA TCCCCATCAA 
     6001 CCACTACCTG AGCACTCAGA CCAAGATCAG CAAGGACCGC AACGAGGCCC GCGACCACAT 
     6061 GGTGCTCCTG GAGTCCTTCA GCGCCTGCTG CCACACCCAC GGCATGGACG AGCTGTACAG 
     6121 GTCCGGACTC AGATAAGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6181 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6241 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6301 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6361 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6421 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6481 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6541 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6601 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6661 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6721 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6781 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     6841 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     6901 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     6961 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7021 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7081 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7141 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7201 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7261 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7321 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7381 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7441 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7501 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7561 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7621 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7681 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7741 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7801 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     7861 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     7921 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     7981 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8041 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8101 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8161 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8221 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8281 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8341 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8401 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8461 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8521 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8581 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8641 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8701 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8761 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     8821 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     8881 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     8941 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9001 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     9061 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//