LOCUS       RC203204                7605 bp    DNA     circular  SYN 
DEFINITION  PSMD2 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2)
ACCESSION   NM_002808
VERSION     NM_002808.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3762..3853 
                     /label="Myc-DDK"  
     misc_feature    3883..4468 
                     /label="PolyA signal"  
     misc_feature    3940..3959 
                     /label="Restriction Enzyme"  
     misc_feature    4744..5416 
                     /label="ColE1"  
     misc_feature    5846..6640 
                     /label="Kan/Neo"  
     misc_feature    6675..6977 
                     /label="SV40 ori"  
     misc_feature    7063..7540 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3744..3750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3744 
                     /label="PSMD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGGGA GGCCGGGACA AGGCGCCGGT GCAGCCCCAG CAGTCTCCAG 
     1081 CGGCGGCCCC CGGCGGCACG GACGAGAAGC CGAGCGGCAA GGAGCGGCGG GATGCCGGGG 
     1141 ACAAGGACAA AGAACAGGAG CTGTCTGAAG AGGATAAACA GCTTCAAGAT GAACTGGAGA 
     1201 TGCTCGTGGA ACGACTAGGG GAGAAGGATA CATCCCTGTA TCGACCAGCG CTGGAGGAAT 
     1261 TGCGAAGGCA GATTCGTTCT TCTACAACTT CCATGACTTC AGTGCCCAAG CCTCTCAAAT 
     1321 TTCTGCGTCC ACACTATGGC AAACTGAAGG AAATCTATGA GAACATGGCC CCTGGGGAGA 
     1381 ATAAGCGTTT TGCTGCTGAC ATCATCTCCG TTTTGGCCAT GACCATGAGT GGGGAGCGTG 
     1441 AGTGCCTCAA GTATCGGCTA GTGGGCTCCC AGGAGGAATT GGCATCATGG GGTCATGAGT 
     1501 ATGTCAGGCA TCTGGCAGGA GAAGTGGCTA AGGAGTGGCA GGAGCTGGAT GACGCAGAGA 
     1561 AGGTCCAGCG GGAGCCTCTG CTCACTCTGG TGAAGGAAAT CGTCCCCTAT AACATGGCCC 
     1621 ACAATGCAGA GCATGAGGCT TGCGACCTGC TTATGGAAAT TGAGCAGGTG GACATGCTGG 
     1681 AGAAGGACAT TGATGAAAAT GCATATGCAA AGGTCTGCCT TTATCTCACC AGTTGTGTGA 
     1741 ATTACGTGCC TGAGCCTGAG AACTCAGCCC TACTGCGTTG TGCCCTGGGT GTGTTCCGAA 
     1801 AGTTTAGCCG CTTCCCTGAA GCTCTGAGAT TGGCATTGAT GCTCAATGAC ATGGAGTTGG 
     1861 TAGAAGACAT CTTCACCTCC TGCAAGGATG TGGTAGTACA GAAACAGATG GCATTCATGC 
     1921 TAGGCCGGCA TGGGGTGTTC CTGGAGCTGA GTGAAGATGT CGAGGAGTAT GAGGACCTGA 
     1981 CAGAGATCAT GTCCAATGTA CAGCTCAACA GCAACTTCTT GGCCTTAGCT CGGGAGCTGG 
     2041 ACATCATGGA GCCCAAGGTG CCTGATGACA TCTACAAAAC CCACCTAGAG AACAACAGGT 
     2101 TTGGGGGCAG TGGCTCTCAG GTGGACTCTG CCCGCATGAA CCTGGCCTCC TCTTTTGTGA 
     2161 ATGGCTTTGT GAATGCAGCT TTTGGCCAAG ACAAGCTGCT AACAGATGAT GGCAACAAAT 
     2221 GGCTTTACAA GAACAAGGAC CACGGAATGT TGAGTGCAGC TGCATCTCTT GGGATGATTC 
     2281 TGCTGTGGGA TGTGGATGGT GGCCTCACCC AGATTGACAA GTACCTGTAC TCCTCTGAGG 
     2341 ACTACATTAA GTCAGGAGCT CTTCTTGCCT GTGGCATAGT GAACTCTGGG GTCCGGAATG 
     2401 AGTGTGACCC TGCTCTGGCA CTGCTCTCAG ACTATGTTCT CCACAACAGC AACACCATGA 
     2461 GACTTGGTTC CATCTTTGGG CTAGGCTTGG CTTATGCTGG CTCAAATCGT GAAGATGTCC 
     2521 TAACACTGCT GCTGCCTGTG ATGGGAGATT CAAAGTCCAG CATGGAGGTG GCAGGTGTCA 
     2581 CAGCTTTAGC CTGTGGAATG ATAGCAGTAG GGTCCTGCAA TGGAGATGTA ACTTCCACTA 
     2641 TCCTTCAGAC CATCATGGAG AAGTCAGAGA CTGAGCTCAA GGATACTTAT GCTCGTTGGC 
     2701 TTCCTCTTGG ACTGGGTCTC AACCACCTGG GGAAGGGTGA GGCCATCGAG GCAATCCTGG 
     2761 CTGCACTGGA GGTTGTGTCA GAGCCATTCC GCAGTTTTGC CAACACACTG GTGGATGTGT 
     2821 GTGCATATGC AGGCTCTGGG AATGTGCTGA AGGTGCAGCA GCTGCTCCAC ATTTGTAGCG 
     2881 AACACTTTGA CTCCAAAGAG AAGGAGGAAG ACAAAGACAA GAAGGAAAAG AAAGACAAGG 
     2941 ACAAGAAGGA AGCCCCTGCT GACATGGGAG CACATCAGGG AGTGGCTGTT CTGGGGATTG 
     3001 CCCTTATTGC TATGGGGGAG GAGATTGGTG CAGAGATGGC ATTACGAACC TTTGGCCACT 
     3061 TGCTGAGATA TGGGGAGCCT ACACTCCGGA GGGCTGTACC TTTAGCACTG GCCCTCATCT 
     3121 CTGTTTCAAA TCCACGACTC AACATCCTGG ATACCCTAAG CAAATTCTCT CATGATGCTG 
     3181 ATCCAGAAGT TTCCTATTAC TCCATTTTTG CCATGGGCAT GGTGGGCAGT GGTACCAATA 
     3241 ATGCCCGTCT GGCTGCAATG CTGCGCCAGT TAGCTCAATA TCATGCCAAG GACCCAAACA 
     3301 ACCTCTTCAT GGTGCGCTTG GCACAGGGCC TGACACATTT AGGGAAGGGC ACCCTTACCC 
     3361 TCTGCCCCTA CCACAGCGAC CGGCAGCTTA TGAGCCAGGT GGCCGTGGCT GGACTGCTCA 
     3421 CTGTGCTTGT CTCTTTCCTG GATGTTCGAA ACATTATTCT AGGCAAATCA CACTATGTAT 
     3481 TGTATGGGCT GGTGGCTGCC ATGCAGCCCC GAATGCTGGT TACGTTTGAT GAGGAGCTGC 
     3541 GGCCATTGCC AGTGTCTGTC CGTGTGGGCC AGGCAGTGGA TGTGGTGGGC CAGGCTGGCA 
     3601 AGCCGAAGAC TATCACAGGG TTCCAGACGC ATACAACCCC AGTGTTGTTG GCCCACGGGG 
     3661 AACGGGCAGA ATTGGCCACT GAGGAGTTTC TTCCTGTTAC CCCCATTCTG GAAGGTTTTG 
     3721 TTATCCTTCG GAAGAACCCC AATTATGATC TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3781 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3841 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3901 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3961 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4021 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4081 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4141 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4201 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4261 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4321 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4381 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4441 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4501 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4561 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4621 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4681 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4741 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4801 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4861 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4921 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4981 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5041 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5101 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5161 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5221 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5281 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5341 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5401 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5461 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5521 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5581 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5641 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5701 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5761 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5821 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5881 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5941 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6001 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6061 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6121 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6181 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6241 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6301 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6361 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6421 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6481 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6541 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6601 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6661 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6721 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6781 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6841 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6961 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7021 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7081 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7141 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7201 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7261 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7321 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7381 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7441 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7501 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7561 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//