LOCUS       RC203018                7698 bp    DNA     circular  SYN 
DEFINITION  AP2A2 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 2 subunit (AP2A2)
ACCESSION   NM_012305
VERSION     NM_012305.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3855..3946 
                     /label="Myc-DDK"  
     misc_feature    3976..4561 
                     /label="PolyA signal"  
     misc_feature    4033..4052 
                     /label="Restriction Enzyme"  
     misc_feature    4837..5509 
                     /label="ColE1"  
     misc_feature    5939..6733 
                     /label="Kan/Neo"  
     misc_feature    6768..7070 
                     /label="SV40 ori"  
     misc_feature    7156..7633 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3837..3843 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3837 
                     /label="AP2A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCCGTG TCCAAGGGGG ACGGGATGCG GGGCCTGGCG GTCTTCATCT 
     1081 CGGATATCCG CAACTGTAAA AGTAAAGAAG CAGAAATAAA AAGGATAAAC AAGGAACTGG 
     1141 CAAATATCAG ATCAAAATTT AAAGGTGACA AGGCTCTTGA TGGCTATAGT AAAAAAAAGT 
     1201 ACGTCTGCAA GTTGCTCTTC ATCTTTCTCC TTGGTCATGA CATTGACTTT GGACACATGG 
     1261 AGGCTGTGAA CCTGCTGAGT TCAAACAGAT ACACGGAAAA GCAGATCGGC TACCTTTTCA 
     1321 TCTCTGTGTT GGTGAACTCA AACAGTGAGC TGATCCGCCT GATCAACAAC GCCATCAAGA 
     1381 ATGACCTGGC CAGCCGCAAC CCCACCTTCA TGGGCCTGGC CCTGCACTGC ATCGCCAGCG 
     1441 TGGGCAGCCG GGAGATGGCC GAGGCCTTCG CCGGGGAGAT CCCTAAGGTC CTCGTAGCCG 
     1501 GAGACACTAT GGACAGCGTG AAGCAGAGCG CGGCCCTGTG CTTGCTGCGC CTGTACAGGA 
     1561 CGTCCCCCGA TCTTGTCCCC ATGGGCGACT GGACATCCCG AGTGGTGCAC CTGCTCAATG 
     1621 ACCAGCACTT GGGTGTGGTA ACTGCAGCCA CAAGTCTGAT CACCACTTTA GCACAGAAGA 
     1681 ACCCAGAAGA GTTTAAAACC TCCGTGTCTC TGGCTGTCTC TAGGCTAAGC AGAATCGTGA 
     1741 CGTCTGCATC CACAGATCTC CAGGATTACA CTTACTATTT TGTCCCGGCT CCCTGGCTGT 
     1801 CTGTCAAACT GCTGAGACTG CTGCAGTGCT ACCCACCCCC AGACCCTGCA GTGCGAGGCC 
     1861 GCCTGACTGA GTGCCTGGAG ACCATCCTGA ACAAAGCCCA AGAACCGCCC AAGTCGAAGA 
     1921 AGGTCCAGCA CTCCAACGCG AAGAATGCCG TGCTCTTCGA GGCCATCAGC TTAATCATTC 
     1981 ACCATGACAG TGAGCCGAAC CTGCTCGTCC GTGCCTGCAA CCAGTTGGGC CAGTTTCTGC 
     2041 AGCACCGCGA GACCAACCTG CGCTACCTGG CCCTGGAGAG CATGTGCACG CTGGCCAGCT 
     2101 CTGAGTTCTC CCATGAGGCT GTCAAGACGC ACATCGAGAC GGTCATCAAC GCCCTGAAGA 
     2161 CTGAGCGGGA CGTGAGCGTG CGGCAGCGGG CCGTGGACCT CCTCTACGCC ATGTGCGACC 
     2221 GCAGCAACGC CCCACAGATC GTGGCCGAGA TGCTGAGCTA TCTGGAGACA GCTGACTACT 
     2281 CCATCCGAGA AGAGATTGTG CTGAAGGTCG CCATCCTGGC TGAGAAGTAC GCGGTGGACT 
     2341 ACACCTGGTA TGTGGATACC ATCTTGAACT TGATCCGAAT TGCTGGTGAT TACGTGAGTG 
     2401 AAGAGGTGTG GTACCGAGTC ATTCAGATCG TCATCAACCG GGACGACGTG CAGGGCTACG 
     2461 CGGCCAAGAC TGTGTTCGAG GCTCTTCAGG CTCCCGCGTG CCACGAGAAC CTGGTCAAAG 
     2521 TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGAGACCCG AGATCCAGCC 
     2581 CGCTGATCCA GTTCCACCTG CTGCACTCCA AGTTCCACCT GTGCAGCGTC CCCACCCGCG 
     2641 CGCTGCTCCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT CCCGGAGGTG AAGCCCACCA 
     2701 TCCAGGACGT GCTGCGCAGC GACAGCCAGC TCAGGAACGC AGACGTGGAG CTGCAGCAGC 
     2761 GTGCTGTGGA GTACCTGCGG CTCAGCACCG TGGCCAGCAC CGACATTCTG GCGACCGTGC 
     2821 TGGAGGAGAT GCCCCCATTC CCGGAGCGGG AGTCCTCCAT CTTGGCAAAG CTCAAGAAGA 
     2881 AGAAGGGCCC CAGCACGGTG ACAGACCTGG AGGACACCAA GCGGGACAGG AGTGTGGACG 
     2941 TGAACGGGGG TCCTGAGCCT GCCCCAGCCA GTACCAGCGC CGTGTCTACG CCTTCTCCGT 
     3001 CGGCAGACCT GCTGGGTCTC GGGGCTGCCC CCCCTGCCCC CGCGGGCCCC CCACCCTCCT 
     3061 CCGGCGGCAG CGGGCTGCTC GTGGACGTGT TCTCAGACTC GGCCTCTGTG GTCGCGCCTC 
     3121 TCGCTCCTGG CTCCGAAGAC AACTTTGCCA GGTTTGTTTG TAAAAACAAT GGTGTGTTGT 
     3181 TTGAAAACCA GCTGCTTCAA ATTGGACTTA AGTCTGAATT TCGGCAGAAT TTAGGTCGGA 
     3241 TGTTTATCTT TTATGGTAAT AAGACCTCCA CGCAGTTCCT AAACTTTACC CCAACACTAA 
     3301 TCTGTTCAGA CGACCTTCAG CCTAACCTGA ACCTGCAGAC CAAGCCCGTG GACCCGACCG 
     3361 TGGAGGGGGG CGCGCAGGTG CAGCAGGTGG TCAACATAGA GTGCGTGTCC GACTTCACGG 
     3421 AGGCGCCAGT CCTCAACATT CAGTTCAGGT ATGGGGGCAC CTTCCAGAAC GTGTCTGTGC 
     3481 AGCTGCCCAT CACTCTCAAC AAATTCTTCC AGCCGACAGA AATGGCTTCT CAGGATTTCT 
     3541 TTCAACGTTG GAAGCAGTTG AGCAATCCAC AGCAGGAAGT GCAGAACATC TTCAAAGCAA 
     3601 AGCACCCAAT GGACACAGAA GTCACCAAAG CCAAGATCAT TGGATTTGGT TCTGCACTTC 
     3661 TTGAAGAAGT TGATCCTAAT CCTGCGAATT TCGTGGGAGC TGGAATCATC CACACGAAAA 
     3721 CCACCCAGAT TGGATGCCTG CTGCGCTTGG AGCCGAACCT GCAAGCCCAG ATGTACCGGC 
     3781 TCACGCTGCG CACAAGTAAG GAAGCCGTTT CTCAGAGATT ATGTGAATTG CTCTCAGCGC 
     3841 AGTTTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3901 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3961 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4021 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4081 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4141 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4201 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4261 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4321 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4381 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4441 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4501 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4561 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4621 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4681 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4741 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4801 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4861 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4921 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4981 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5041 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5101 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5161 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5221 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5281 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5341 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5401 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5461 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5521 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5581 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5641 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5701 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5761 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5821 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5881 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5941 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6001 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6061 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6121 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6181 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6241 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6301 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6361 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6421 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6481 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6541 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6601 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6661 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6721 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6781 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6841 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6901 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6961 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7021 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7081 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7141 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7201 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7261 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7321 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7381 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7441 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7501 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7561 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7621 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7681 AAATTTAACG CGAATTTT
//