LOCUS       RC202932                7581 bp    DNA     circular  SYN 
DEFINITION  MARS (Myc-DDK-tagged)-Human methionyl-tRNA synthetase (MARS)
ACCESSION   NM_004990
VERSION     NM_004990.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3738..3829 
                     /label="Myc-DDK"  
     misc_feature    3859..4444 
                     /label="PolyA signal"  
     misc_feature    3916..3935 
                     /label="Restriction Enzyme"  
     misc_feature    4720..5392 
                     /label="ColE1"  
     misc_feature    5822..6616 
                     /label="Kan/Neo"  
     misc_feature    6651..6953 
                     /label="SV40 ori"  
     misc_feature    7039..7516 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3720..3726 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3720 
                     /label="MARS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACTGTTC GTGAGTGATG GCGTCCCGGG TTGCTTGCCG GTGCTGGCCG 
     1081 CCGCCGGGAG AGCCCGGGGC AGAGCAGAGG TGCTCATCAG CACTGTAGGC CCGGAAGATT 
     1141 GTGTGGTCCC GTTCCTGACC CGGCCTAAGG TCCCTGTCTT GCAGCTGGAT AGCGGCAACT 
     1201 ACCTCTTCTC CACTAGTGCA ATCTGCCGAT ATTTTTTTTT GTTATCTGGC TGGGAGCAAG 
     1261 ATGACCTCAC TAACCAGTGG CTGGAATGGG AAGCGACAGA GCTGCAGCCA GCTTTGTCTG 
     1321 CTGCCCTGTA CTATTTAGTG GTCCAAGGCA AGAAGGGGGA AGATGTTCTT GGTTCAGTGC 
     1381 GGAGAGCCCT GACTCACATT GACCACAGCT TGAGTCGTCA GAACTGTCCT TTCCTGGCTG 
     1441 GGGAGACAGA ATCTCTAGCC GACATTGTTT TGTGGGGAGC CCTATACCCA TTACTGCAAG 
     1501 ATCCCGCCTA CCTCCCTGAG GAGCTGAGTG CCCTGCACAG CTGGTTCCAG ACACTGAGTA 
     1561 CCCAGGAACC ATGTCAGCGA GCTGCAGAGA CTGTACTGAA ACAGCAAGGT GTCCTGGCTC 
     1621 TCCGGCCTTA CCTCCAAAAG CAGCCCCAGC CCAGCCCCGC TGAGGGAAGG GCTGTCACCA 
     1681 ATGAGCCTGA GGAGGAGGAG CTGGCTACCC TATCTGAGGA GGAGATTGCT ATGGCTGTTA 
     1741 CTGCTTGGGA GAAGGGCCTA GAAAGTTTGC CCCCGCTGCG GCCCCAGCAG AATCCAGTGT 
     1801 TGCCTGTGGC TGGAGAAAGG AATGTGCTCA TCACCAGTGC CCTCCCTTAC GTCAACAATG 
     1861 TCCCCCACCT TGGGAACATC ATTGGTTGTG TGCTCAGTGC CGATGTCTTT GCCAGGTACT 
     1921 CTCGCCTCCG CCAGTGGAAC ACCCTCTATC TGTGTGGGAC AGATGAGTAT GGTACAGCAA 
     1981 CAGAGACCAA GGCTCTGGAG GAGGGACTAA CCCCCCAGGA GATCTGCGAC AAGTACCACA 
     2041 TCATCCATGC TGACATCTAC CGCTGGTTTA ACATTTCGTT TGATATTTTT GGTCGCACCA 
     2101 CCACTCCACA GCAGACCAAA ATCACCCAGG ACATTTTCCA GCAGTTGCTG AAACGAGGTT 
     2161 TTGTGCTGCA AGATACTGTG GAGCAACTGC GATGTGAGCA CTGTGCTCGC TTCCTGGCTG 
     2221 ACCGCTTCGT GGAGGGCGTG TGTCCCTTCT GTGGCTATGA GGAGGCTCGG GGTGACCAGT 
     2281 GTGACAAGTG TGGCAAGCTC ATCAATGCTG TCGAGCTTAA GAAGCCTCAG TGTAAAGTCT 
     2341 GCCGATCATG CCCTGTGGTG CAGTCGAGCC AGCACCTGTT TCTGGACCTG CCTAAGCTGG 
     2401 AGAAGCGACT GGAGGAGTGG TTGGGGAGGA CATTGCCTGG CAGTGACTGG ACACCCAATG 
     2461 CCCAGTTTAT CACCCGTTCT TGGCTTCGGG ATGGCCTCAA GCCACGCTGC ATAACCCGAG 
     2521 ACCTCAAATG GGGAACCCCT GTACCCTTAG AAGGTTTTGA AGACAAGGTA TTCTATGTCT 
     2581 GGTTTGATGC CACTATTGGC TATCTGTCCA TCACAGCCAA CTACACAGAC CAGTGGGAGA 
     2641 GATGGTGGAA GAACCCAGAG CAAGTGGACC TGTATCAGTT CATGGCCAAA GACAATGTTC 
     2701 CTTTCCATAG CTTAGTCTTT CCTTGCTCAG CCCTAGGAGC TGAGGATAAC TATACCTTGG 
     2761 TCAGCCACCT CATTGCTACA GAGTACCTGA ACTATGAGGA TGGGAAATTC TCTAAGAGCC 
     2821 GCGGTGTGGG AGTGTTTGGG GACATGGCCC AGGACACGGG GATCCCTGCT GACATCTGGC 
     2881 GCTTCTATCT GCTGTACATT CGGCCTGAGG GCCAGGACAG TGCTTTCTCC TGGACGGACC 
     2941 TGCTGCTGAA GAATAATTCT GAGCTGCTTA ACAACCTGGG CAACTTCATC AACAGAGCTG 
     3001 GGATGTTTGT GTCTAAGTTC TTTGGGGGCT ATGTGCCTGA GATGGTGCTC ACCCCTGATG 
     3061 ATCAGCGCCT GCTGGCCCAT GTCACCCTGG AGCTCCAGCA CTATCACCAG CTACTTGAGA 
     3121 AGGTTCGGAT CCGGGATGCC TTGCGCAGTA TCCTCACCAT ATCTCGACAT GGCAACCAAT 
     3181 ATATTCAGGT GAATGAGCCC TGGAAGCGGA TTAAAGGCAG TGAGGCTGAC AGGCAACGGG 
     3241 CAGGAACAGT GACTGGCTTG GCAGTGAATA TAGCTGCCTT GCTCTCTGTC ATGCTTCAGC 
     3301 CTTACATGCC CACGGTTAGT GCCACAATCC AGGCCCAGCT GCAGCTCCCA CCTCCAGCCT 
     3361 GCAGTATCCT GCTGACAAAC TTCCTGTGTA CCTTACCAGC AGGACACCAG ATTGGCACAG 
     3421 TCAGTCCCTT GTTCCAAAAA TTGGAAAATG ACCAGATTGA AAGTTTAAGG CAGCGCTTTG 
     3481 GAGGGGGCCA GGCAAAAACG TCCCCGAAGC CAGCAGTTGT AGAGACTGTT ACAACAGCCA 
     3541 AGCCACAGCA GATACAAGCG CTGATGGATG AAGTGACAAA ACAAGGAAAC ATTGTCCGAG 
     3601 AACTGAAAGC ACAAAAGGCA GACAAGAACG AGGTTGCTGC GGAGGTGGCG AAACTCTTGG 
     3661 ATCTAAAGAA ACAGTTGGCT GTAGCTGAGG GGAAACCCCC TGAAGCCCCT AAAGGCAAGA 
     3721 AGAAAAAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3781 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3841 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3901 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3961 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4021 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4081 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4141 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4201 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4261 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4321 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4381 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4441 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4501 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4561 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4621 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4681 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4741 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4801 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4861 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4921 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4981 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5041 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5101 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5161 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5221 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5281 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5341 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5401 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5461 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5521 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5581 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5641 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5701 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5761 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5821 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5881 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5941 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6001 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6061 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6121 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6181 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6241 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6301 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6361 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6421 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6481 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6541 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6601 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6661 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6721 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6781 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6841 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6901 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6961 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7021 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7081 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7141 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7201 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7261 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7321 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7381 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7441 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7501 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7561 CAAAAATTTA ACGCGAATTT T
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