LOCUS       RC202890                7368 bp    DNA     circular  SYN 
DEFINITION  MCC (Myc-DDK-tagged)-Human mutated in colorectal cancers (MCC), transcript variant 2
ACCESSION   NM_002387
VERSION     NM_002387.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3525..3616 
                     /label="Myc-DDK"  
     misc_feature    3646..4231 
                     /label="PolyA signal"  
     misc_feature    3703..3722 
                     /label="Restriction Enzyme"  
     misc_feature    4507..5179 
                     /label="ColE1"  
     misc_feature    5609..6403 
                     /label="Kan/Neo"  
     misc_feature    6438..6740 
                     /label="SV40 ori"  
     misc_feature    6826..7303 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3507..3513 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3507 
                     /label="MCC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTCCGGA GTTGCCATGA AATATGGAAA CGACTCCTCG GCCGAGCTGA 
     1081 GTGAGCTCCA TTCAGCAGCC CTGGCATCAC TAAAGGGAGA TATAGTGGAA CTTAATAAAC 
     1141 GTCTCCAGCA AACAGAGAGG GAACGGGACC TTCTGGAAAA GAAATTGGCC AAGGCACAGT 
     1201 GCGAGCAGTC CCACCTCATG AGAGAGCATG AGGATGTCCA GGAGCGAACG ACGCTTCGCT 
     1261 ATGAGGAACG CATCACAGAG CTCCACAGCG TCATTGCGGA GCTCAACAAG AAGATAGACC 
     1321 GTCTGCAAGG CACCACCATC AGGGAGGAAG ATGAGTACTC AGAACTGCGA TCAGAACTCA 
     1381 GCCAGAGCCA ACACGAGGTC AACGAGGACT CTCGAAGCAT GGACCAAGAC CAGACCTCTG 
     1441 TCTCTATCCC CGAAAACCAG TCTACCATGG TTACTGCTGA CATGGACAAC TGCAGTGACC 
     1501 TGAACTCAGA ACTGCAGAGG GTGCTGACAG GGCTGGAGAA TGTTGTCTGC GGCAGGAAGA 
     1561 AGAGCAGCTG CAGCCTCTCC GTGGCCGAGG TGGACAGGCA CATTGAGCAG CTCACCACAG 
     1621 CCAGCGAGCA CTGTGACCTG GCTATTAAGA CAGTCGAGGA GATTGAGGGG GTGCTTGGCC 
     1681 GGGACCTGTA TCCCAACCTG GCTGAAGAGA GGTCTCGGTG GGAGAAGGAG CTGGCTGGGC 
     1741 TGAGGGAAGA GAATGAGAGC CTGACTGCCA TGCTGTGCAG CAAAGAGGAA GAACTGAACC 
     1801 GGACTAAGGC CACCATGAAT GCCATCCGGG AAGAGCGGGA CCGGCTCCGG AGGAGGGTCA 
     1861 GAGAGCTTCA AACTCGACTA CAGAGCGTGC AGGCCACAGG TCCCTCCAGC CCTGGCCGCC 
     1921 TCACTTCCAC CAACCGCCCG ATTAACCCCA GCACTGGGGA GCTGAGCACA AGCAGCAGCA 
     1981 GCAATGACAT TCCCATCGCC AAGATTGCTG AGAGGGTGAA GCTATCAAAG ACAAGGTCCG 
     2041 AATCGTCATC ATCTGATCGG CCAGTCCTGG GCTCAGAAAT CAGTAGCATA GGGGTATCCA 
     2101 GCAGTGTGGC TGAACACCTG GCCCACTCAC TTCAGGACTG CTCCAATATC CAAGAGATTT 
     2161 TCCAAACACT CTACTCACAC GGATCTGCCA TCTCAGAAAG CAAGATTAGA GAGTTTGAGG 
     2221 TGGAAACAGA ACGGCTGAAT AGCCGGATTG AGCACCTCAA ATCCCAAAAT GACCTCCTGA 
     2281 CCATAACCTT GGAGGAATGT AAAAGCAATG CCGAGAGGAT GAGCATGCTG GTGGGAAAAT 
     2341 ACGAATCCAA TGCCACAGCG CTGAGGCTGG CCTTGCAGTA CAGCGAGCAG TGCATCGAAG 
     2401 CCTACGAACT CCTCCTGGCG CTGGCAGAGA GTGAGCAGAG CCTCATCCTG GGGCAGTTCC 
     2461 GAGCGGCGGG CGTGGGGTCC TCCCCTGGAG ACCAGTCGGG GGATGAAAAC ATCACTCAGA 
     2521 TGCTCAAGCG AGCTCATGAC TGCCGGAAGA CAGCTGAGAA TGCTGCCAAG GCCCTGCTCA 
     2581 TGAAGCTGGA CGGCAGCTGT GGGGGAGCCT TTGCCGTGGC CGGCTGCAGC GTGCAGCCCT 
     2641 GGGAGAGCCT TTCCTCCAAC AGCCACACCA GCACAACCAG CTCCACAGCC AGTAGTTGCG 
     2701 ACACCGAGTT CACTAAAGAA GACGAGCAGA GGCTGAAGGA TTATATCCAG CAGCTCAAGA 
     2761 ATGACAGGGC TGCGGTCAAG CTGACCATGC TGGAGCTGGA AAGCATCCAC ATCGATCCTC 
     2821 TCAGCTATGA CGTCAAGCCT CGGGGAGACA GCCAGAGGCT GGATCTGGAA AACGCAGTGC 
     2881 TTATGCAGGA GCTCATGGCC ATGAAGGAGG AGATGGCCGA GTTGAAGGCC CAGCTCTACC 
     2941 TACTGGAGAA AGAGAAGAAG GCCCTGGAGC TGAAGCTGAG CACGCGGGAG GCCCAGGAGC 
     3001 AGGCCTACCT GGTGCACATT GAGCACCTGA AGTCCGAGGT GGAGGAGCAG AAGGAGCAGC 
     3061 GGATGCGATC CCTCAGCTCC ACCAGCAGCG GCAGCAAAGA TAAACCTGGC AAGGAGTGTG 
     3121 CTGATGCTGC CTCCCCAGCT CTGTCCCTAG CTGAACTCAG GACAACGTGC AGCGAGAATG 
     3181 AGCTGGCTGC GGAGTTCACC AACGCCATTC GTCGAGAAAA GAAGTTGAAG GCCAGAGTTC 
     3241 AAGAGCTGGT GAGTGCCTTG GAGAGACTCA CCAAGAGCAG TGAAATCCGA CATCAGCAAT 
     3301 CTGCAGAGTT CGTGAATGAT CTAAAGCGGG CCAACAGCAA CCTGGTGGCT GCCTATGAGA 
     3361 AAGCAAAGAA AAAGCATCAA AACAAACTGA AGAAGTTAGA GTCGCAGATG ATGGCCATGG 
     3421 TGGAGAGACA TGAGACCCAA GTGAGGATGC TCAAGCAAAG AATAGCTCTG CTAGAGGAGG 
     3481 AGAACTCCAG GCCACACACC AATGAAACTT CGCTTACGCG TACGCGGCCG CTCGAGCAGA 
     3541 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3601 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3661 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3721 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3781 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3841 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3901 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3961 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4021 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4081 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4141 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4201 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4261 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4321 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4381 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4441 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4501 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4561 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4621 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4681 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4741 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4801 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4861 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4921 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4981 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5041 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5101 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5161 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5221 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5281 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5341 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5401 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5461 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5521 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5581 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5641 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5701 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5761 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5821 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5881 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5941 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6001 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6061 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6121 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6181 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6241 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6301 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6361 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6421 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6481 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6541 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6601 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6661 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6721 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6781 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6841 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6901 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6961 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7021 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7081 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7141 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7201 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7261 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7321 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//