LOCUS       RC202532L1                8238 bp    DNA     circular  SYN 
DEFINITION  MSLN (Myc-DDK-tagged)-Human mesothelin (MSLN), transcript variant 1
ACCESSION   NM_005823
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6529..6709 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7437..8096 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    6868..7436 
                     /label="ColE1"  
     misc_feature    5404..5424 
                     /label="LR50 primer"  
     misc_feature    5201..5272 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5413..6004 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5183..5189 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5183 
                     /label="MSLN"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCTTGCCA ACGGCTCGAC CCCTGTTGGG 
     3361 GTCCTGTGGG ACCCCCGCCC TCGGCAGCCT CCTGTTCCTG CTCTTCAGCC TCGGATGGGT 
     3421 GCAGCCCTCG AGGACCCTGG CTGGAGAGAC AGGGCAGGCT GCACCCCTGG ACGGAGTCCT 
     3481 GGCCAACCCA CCTAACATTT CCAGCCTCTC CCCTCGCCAA CTCCTTGGCT TCCCGTGTGC 
     3541 GGAGGTGTCC GGCCTGAGCA CGGAGCGTGT CCGGGAGCTG GCTGTGGCCT TGGCACAGAA 
     3601 GAATGTCAAG CTCTCAACAG AGCAGCTGCG CTGTCTGGCT CACCGGCTCT CTGAGCCCCC 
     3661 CGAGGACCTG GACGCCCTCC CATTGGACCT GCTGCTATTC CTCAACCCAG ATGCGTTCTC 
     3721 GGGGCCCCAG GCCTGCACCC GTTTCTTCTC CCGCATCACG AAGGCCAATG TGGACCTGCT 
     3781 CCCGAGGGGG GCTCCCGAGC GACAGCGGCT GCTGCCTGCG GCTCTGGCCT GCTGGGGTGT 
     3841 GCGGGGGTCT CTGCTGAGCG AGGCTGATGT GCGGGCTCTG GGAGGCCTGG CTTGCGACCT 
     3901 GCCTGGGCGC TTTGTGGCCG AGTCGGCCGA AGTGCTGCTA CCCCGGCTGG TGAGCTGCCC 
     3961 GGGACCCCTG GACCAGGACC AGCAGGAGGC AGCCAGGGCG GCTCTGCAGG GCGGGGGACC 
     4021 CCCCTACGGC CCCCCGTCGA CATGGTCTGT CTCCACGATG GACGCTCTGC GGGGCCTGCT 
     4081 GCCCGTGCTG GGCCAGCCCA TCATCCGCAG CATCCCGCAG GGCATCGTGG CCGCGTGGCG 
     4141 GCAACGCTCC TCTCGGGACC CATCCTGGCG GCAGCCTGAA CGGACCATCC TCCGGCCGCG 
     4201 GTTCCGGCGG GAAGTGGAGA AGACAGCCTG TCCTTCAGGC AAGAAGGCCC GCGAGATAGA 
     4261 CGAGAGCCTC ATCTTCTACA AGAAGTGGGA GCTGGAAGCC TGCGTGGATG CGGCCCTGCT 
     4321 GGCCACCCAG ATGGACCGCG TGAACGCCAT CCCCTTCACC TACGAGCAGC TGGACGTCCT 
     4381 AAAGCATAAA CTGGATGAGC TCTACCCACA AGGTTACCCC GAGTCTGTGA TCCAGCACCT 
     4441 GGGCTACCTC TTCCTCAAGA TGAGCCCTGA GGACATTCGC AAGTGGAATG TGACGTCCCT 
     4501 GGAGACCCTG AAGGCTTTGC TTGAAGTCAA CAAAGGGCAC GAAATGAGTC CTCAGGTGGC 
     4561 CACCCTGATC GACCGCTTTG TGAAGGGAAG GGGCCAGCTA GACAAAGACA CCCTAGACAC 
     4621 CCTGACCGCC TTCTACCCTG GGTACCTGTG CTCCCTCAGC CCCGAGGAGC TGAGCTCCGT 
     4681 GCCCCCCAGC AGCATCTGGG CGGTCAGGCC CCAGGACCTG GACACGTGTG ACCCAAGGCA 
     4741 GCTGGACGTC CTCTATCCCA AGGCCCGCCT TGCTTTCCAG AACATGAACG GGTCCGAATA 
     4801 CTTCGTGAAG ATCCAGTCCT TCCTGGGTGG GGCCCCCACG GAGGATTTGA AGGCGCTCAG 
     4861 TCAGCAGAAT GTGAGCATGG ACTTGGCCAC GTTCATGAAG CTGCGGACGG ATGCGGTGCT 
     4921 GCCGTTGACT GTGGCTGAGG TGCAGAAACT TCTGGGACCC CACGTGGAGG GCCTGAAGGC 
     4981 GGAGGAGCGG CACCGCCCGG TGCGGGACTG GATCCTACGG CAGCGGCAGG ACGACCTGGA 
     5041 CACGCTGGGG CTGGGGCTAC AGGGCGGCAT CCCCAACGGC TACCTGGTCC TAGACCTCAG 
     5101 CGTGCAAGAG GCCCTCTCGG GGACGCCCTG CCTCCTAGGA CCTGGACCTG TTCTCACCGT 
     5161 CCTGGCACTG CTCCTAGCCT CCACCCTGGC CACGCGTACG CGGCCGCTCG AGCAGAAACT 
     5221 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     5281 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     5341 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     5401 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     5461 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     5521 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     5581 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     5641 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     5701 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     5761 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     5821 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     5881 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     5941 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     6001 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     6061 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     6121 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     6181 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     6241 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     6301 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     6361 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     6421 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     6481 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     6541 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     6601 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     6661 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     6721 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6781 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6841 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6901 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6961 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     7021 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     7081 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     7141 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     7201 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     7261 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     7321 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     7381 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     7441 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     7501 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     7561 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     7621 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     7681 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     7741 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     7801 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     7861 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     7921 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     7981 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     8041 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     8101 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8161 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 
     8221 CGAAAAGTGC CACCTGAC
//