LOCUS       RC202532                6744 bp    DNA     circular  SYN 
DEFINITION  MSLN (Myc-DDK-tagged)-Human mesothelin (MSLN), transcript variant 1
ACCESSION   NM_005823
VERSION     NM_005823.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2901..2992 
                     /label="Myc-DDK"  
     misc_feature    3022..3607 
                     /label="PolyA signal"  
     misc_feature    3079..3098 
                     /label="Restriction Enzyme"  
     misc_feature    3883..4555 
                     /label="ColE1"  
     misc_feature    4985..5779 
                     /label="Kan/Neo"  
     misc_feature    5814..6116 
                     /label="SV40 ori"  
     misc_feature    6202..6679 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2883..2889 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2883 
                     /label="MSLN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTTGCCA ACGGCTCGAC CCCTGTTGGG GTCCTGTGGG ACCCCCGCCC 
     1081 TCGGCAGCCT CCTGTTCCTG CTCTTCAGCC TCGGATGGGT GCAGCCCTCG AGGACCCTGG 
     1141 CTGGAGAGAC AGGGCAGGCT GCACCCCTGG ACGGAGTCCT GGCCAACCCA CCTAACATTT 
     1201 CCAGCCTCTC CCCTCGCCAA CTCCTTGGCT TCCCGTGTGC GGAGGTGTCC GGCCTGAGCA 
     1261 CGGAGCGTGT CCGGGAGCTG GCTGTGGCCT TGGCACAGAA GAATGTCAAG CTCTCAACAG 
     1321 AGCAGCTGCG CTGTCTGGCT CACCGGCTCT CTGAGCCCCC CGAGGACCTG GACGCCCTCC 
     1381 CATTGGACCT GCTGCTATTC CTCAACCCAG ATGCGTTCTC GGGGCCCCAG GCCTGCACCC 
     1441 GTTTCTTCTC CCGCATCACG AAGGCCAATG TGGACCTGCT CCCGAGGGGG GCTCCCGAGC 
     1501 GACAGCGGCT GCTGCCTGCG GCTCTGGCCT GCTGGGGTGT GCGGGGGTCT CTGCTGAGCG 
     1561 AGGCTGATGT GCGGGCTCTG GGAGGCCTGG CTTGCGACCT GCCTGGGCGC TTTGTGGCCG 
     1621 AGTCGGCCGA AGTGCTGCTA CCCCGGCTGG TGAGCTGCCC GGGACCCCTG GACCAGGACC 
     1681 AGCAGGAGGC AGCCAGGGCG GCTCTGCAGG GCGGGGGACC CCCCTACGGC CCCCCGTCGA 
     1741 CATGGTCTGT CTCCACGATG GACGCTCTGC GGGGCCTGCT GCCCGTGCTG GGCCAGCCCA 
     1801 TCATCCGCAG CATCCCGCAG GGCATCGTGG CCGCGTGGCG GCAACGCTCC TCTCGGGACC 
     1861 CATCCTGGCG GCAGCCTGAA CGGACCATCC TCCGGCCGCG GTTCCGGCGG GAAGTGGAGA 
     1921 AGACAGCCTG TCCTTCAGGC AAGAAGGCCC GCGAGATAGA CGAGAGCCTC ATCTTCTACA 
     1981 AGAAGTGGGA GCTGGAAGCC TGCGTGGATG CGGCCCTGCT GGCCACCCAG ATGGACCGCG 
     2041 TGAACGCCAT CCCCTTCACC TACGAGCAGC TGGACGTCCT AAAGCATAAA CTGGATGAGC 
     2101 TCTACCCACA AGGTTACCCC GAGTCTGTGA TCCAGCACCT GGGCTACCTC TTCCTCAAGA 
     2161 TGAGCCCTGA GGACATTCGC AAGTGGAATG TGACGTCCCT GGAGACCCTG AAGGCTTTGC 
     2221 TTGAAGTCAA CAAAGGGCAC GAAATGAGTC CTCAGGTGGC CACCCTGATC GACCGCTTTG 
     2281 TGAAGGGAAG GGGCCAGCTA GACAAAGACA CCCTAGACAC CCTGACCGCC TTCTACCCTG 
     2341 GGTACCTGTG CTCCCTCAGC CCCGAGGAGC TGAGCTCCGT GCCCCCCAGC AGCATCTGGG 
     2401 CGGTCAGGCC CCAGGACCTG GACACGTGTG ACCCAAGGCA GCTGGACGTC CTCTATCCCA 
     2461 AGGCCCGCCT TGCTTTCCAG AACATGAACG GGTCCGAATA CTTCGTGAAG ATCCAGTCCT 
     2521 TCCTGGGTGG GGCCCCCACG GAGGATTTGA AGGCGCTCAG TCAGCAGAAT GTGAGCATGG 
     2581 ACTTGGCCAC GTTCATGAAG CTGCGGACGG ATGCGGTGCT GCCGTTGACT GTGGCTGAGG 
     2641 TGCAGAAACT TCTGGGACCC CACGTGGAGG GCCTGAAGGC GGAGGAGCGG CACCGCCCGG 
     2701 TGCGGGACTG GATCCTACGG CAGCGGCAGG ACGACCTGGA CACGCTGGGG CTGGGGCTAC 
     2761 AGGGCGGCAT CCCCAACGGC TACCTGGTCC TAGACCTCAG CGTGCAAGAG GCCCTCTCGG 
     2821 GGACGCCCTG CCTCCTAGGA CCTGGACCTG TTCTCACCGT CCTGGCACTG CTCCTAGCCT 
     2881 CCACCCTGGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     2941 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3001 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3061 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3121 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3181 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3241 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3301 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3361 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3421 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3481 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3541 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3601 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3661 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3721 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3781 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3841 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     3901 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     3961 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4021 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4081 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4141 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4201 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4261 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4321 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4381 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4441 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4501 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4561 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4621 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4681 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4741 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4801 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4861 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     4921 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     4981 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5041 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5101 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5161 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5221 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5281 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5341 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5401 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5461 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5521 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5581 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5641 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5701 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5761 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5821 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     5881 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     5941 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6001 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6061 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6121 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6181 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6241 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6301 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6361 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6421 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6481 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6541 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6601 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6661 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6721 TAACAAAAAT TTAACGCGAA TTTT
//