LOCUS       RC202517                7065 bp    DNA     circular  SYN 
DEFINITION  HOOK1 (Myc-DDK-tagged)-Human hook homolog 1 (Drosophila) (HOOK1)
ACCESSION   NM_015888
VERSION     NM_015888.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3222..3313 
                     /label="Myc-DDK"  
     misc_feature    3343..3928 
                     /label="PolyA signal"  
     misc_feature    3400..3419 
                     /label="Restriction Enzyme"  
     misc_feature    4204..4876 
                     /label="ColE1"  
     misc_feature    5306..6100 
                     /label="Kan/Neo"  
     misc_feature    6135..6437 
                     /label="SV40 ori"  
     misc_feature    6523..7000 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3204..3210 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3204 
                     /label="HOOK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGACG CAGCCGCCGC CGCAGCCTAA GCTGCCCCTG TGCGACAGCC 
     1081 TCATGATCTG GCTGCAGACA TTCAATACTG CCTCACCTTG TCAAGATGTC AAACAGCTGA 
     1141 CTAGTGGAGT TGCCATGGCA CAAGTTCTTC ATCAAATTGA TGCAGCTTGG TTTAACGAAT 
     1201 CTTGGTTAAG CCGAATTAAA GAGGATGTTG GGGACAACTG GAGAATAAAG GCCAGTAATG 
     1261 TAAAGAAGGT CCTTCAAGGA ATTATGAGTT ATTATCATGA GTTTTTGGGG CAGCAGATTT 
     1321 CAGAAGCACT TATCCCTGAT TTAAACCAAA TAACCGAATG TTCAGATCCA GTGGAGCTTG 
     1381 GGAGGTTGCT CCAGCTTATT TTAGGTTGTG CGATCAACTG TGAAAAGAAG CAAGAACATA 
     1441 TTCAAAATAT AATGACACTG GAAGAGTCTG TTCAACATGT GGTCATGACT GCTATTCAAG 
     1501 AGTTGATGAG TAAAGAAATA TTGAGCTCTC CTCCAAATGA TGCTGTTGGA GAATTGGAGC 
     1561 AACAGCTTAA AAGAGCCTTG GAAGAACTTC AGGAAGCACT AGCAGAAAAA GAAGAGCTGA 
     1621 GGCAAAGATG TGAAGAATTG GATATGCAGG TGACTACACT TCAAGATGAA AAGAATTCAC 
     1681 TGGTTTCTGA AAATGAGATG ATGAATGAAA AACTTGACCA GTTGGATGGC TCTTTTGATG 
     1741 ATCCAAACAC AGTGGTTGCA AAAAAGTATT TTCATGCACA ATTACAACTA GAACAATTAC 
     1801 AGGAAGAAAA CTTCAGGCTT GAAGCTGCAA AAGATGATTA CCGTGTTCAC TGTGAAGAAC 
     1861 TTGAAAAGCA GCTAATCGAA TTCCAGCATA GGAATGATGA ATTGACTAGT CTTGCAGAAG 
     1921 AAACAAGAGC CCTGAAAGAT GAAATAGATG TTCTTAGGGC TACCTCTGAT AAAGCAAATA 
     1981 AACTGGAGTC AACAGTTGAG ATATATCGTC AGAAGCTACA AGATCTGAAT GACCTTCGCA 
     2041 AGCAGGTGAA AACTTTACAG GAAACCAACA TGATGTATAT GCATAATACA GTCAGCTTAG 
     2101 AAGAAGAATT AAAAAAAGCA AATGCAGCAC GTACACAATT AGAAACATAC AAAAGGCAGG 
     2161 TTCAAGATCT TCATGTTAAA CTTTCCTCCG AATCCAAGAG GGCAGACACA CTAGCGTTTG 
     2221 AAATGAAGCG GCTTGAAGAA AAACATGAAG CTTTACTTAA GGAAAAAGAG AGACTAATTG 
     2281 AGCAGCGTGA TACTTTGAAA GAAACAAATG AAGAGCTTCG ATGTTCACAA GTACAACAGG 
     2341 ACCACCTAAA CCAAACAGAT GCATCTGCTA CAAAAAGTTA TGAGAATCTT GCTGCTGAGA 
     2401 TTATGCCAGT GGAATATAGG GAGGTGTTTA TTCGACTGCA ACATGAAAAT AAGATGCTTC 
     2461 GCTTACAGCA AGAAGGCTCT GAGAATGAAC GTATTGAGGA ACTTCAGGAG CAGCTAGAAC 
     2521 AGAAACACCG TAAAATGAAT GAACTGGAAA CTGAGCAGAG GCTGAGCAAA GAGCGTATTA 
     2581 GAGAATTGCA GCAGCAGATT GAGGACCTCC AGAAATCTTT ACAGGAACAA GGTTCCAAGT 
     2641 CTGAAGGCGA AAGTTCCAGC AAATTAAAGC AGAAGTTGGA AGCTCATATG GAAAAACTCA 
     2701 CAGAGGTCCA TGAAGAATTA CAGAAGAAAC AAGAACTCAT TGAAGATCTT CAGCCAGATA 
     2761 TAAATCAAAA TGTACAAAAG ATCAATGAAC TTGAAGCTGC TCTTCAGAAG AAAGATGAAG 
     2821 ATATGAAAGC AATGGAGGAA AGATATAAAA TGTACTTGGA GAAAGCCAGA AATGTAATAA 
     2881 AAACTTTGGA TCCCAAGTTA AATCCAGCAT CAGCTGAAAT AATGCTACTA AGAAAGCAGT 
     2941 TGGCAGAGAA AGAGAGAAGA ATTGAGATTC TGGAGAGTGA ATGCAAAGTA GCAAAATTCC 
     3001 GTGATTATGA AGAAAAACTC ATTGTTTCTG CGTGGTATAA TAAGAGTCTA GCATTCCAGA 
     3061 AACTGGGGAT GGAATCTAGA CTTGTGAGCG GCGGTGGTGC CTGCAGTGAC ACTGGTGCGT 
     3121 GCACTCCTGC GCGGTCTTTC TTAGCGCAGC AACGGCACAT CACCAACACC AGAAGAAATC 
     3181 TCTCTGTTAA AGTCCCTGCT ACAACATCTG ATACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3241 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3301 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3361 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3421 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3481 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3541 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3601 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3661 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3721 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3781 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3841 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3901 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3961 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4021 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4081 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4141 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4201 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4261 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4321 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4381 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4441 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4501 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4561 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4621 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4681 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4741 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4801 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4861 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4921 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4981 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5041 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5101 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5161 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5221 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5281 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5341 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5401 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5461 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5521 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5581 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5641 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5701 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5761 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5821 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5881 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5941 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6001 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6061 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6121 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6181 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6241 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6301 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6361 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6481 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6541 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6601 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6661 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6721 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6781 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6841 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6901 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6961 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7021 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//