LOCUS       RC201960                8265 bp    DNA     circular  SYN 
DEFINITION  NCKAP1 (Myc-DDK-tagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 1
ACCESSION   NM_013436
VERSION     NM_013436.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4422..4513 
                     /label="Myc-DDK"  
     misc_feature    4543..5128 
                     /label="PolyA signal"  
     misc_feature    4600..4619 
                     /label="Restriction Enzyme"  
     misc_feature    5404..6076 
                     /label="ColE1"  
     misc_feature    6506..7300 
                     /label="Kan/Neo"  
     misc_feature    7335..7637 
                     /label="SV40 ori"  
     misc_feature    7723..8200 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4404..4410 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4404 
                     /label="NCKAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCGCTCA GTGCTGCAGC CCAGTCAGCA GAAGCTGGCG GAGAAGCTCA 
     1081 CCATCCTCAA CGACCGGGGC GTCGGCATGC TCACCCGCCT CTACAACATC AAGAAGGCAT 
     1141 GTGGAGACCC CAAGGCAAAA CCATCCTATC TTATCGACAA AAACCTGGAA TCTGCTGTGA 
     1201 AATTCATAGT CAGAAAATTC CCTGCTGTAG AAACCCGCAA CAACAATCAA CAGCTTGCAC 
     1261 AACTACAGAA AGAAAAATCA GAGATTCTGA AAAATCTGGC ATTATATTAC TTCACATTTG 
     1321 TAGATGTTAT GGAATTTAAG GACCATGTTT GTGAATTGCT GAATACTATT GACGTTTGCC 
     1381 AAGTCTTCTT TGATATTACT GTAAACTTTG ATTTAACAAA GAACTACTTA GATTTAATTA 
     1441 TAACCTATAC AACACTAATG ATACTGCTGT CTCGAATTGA AGAAAGGAAG GCAATCATTG 
     1501 GATTATACAA CTATGCCCAT GAAATGACTC ATGGAGCAAG TGACAGAGAA TACCCACGCC 
     1561 TTGGCCAGAT GATTGTGGAT TATGAAAACC CTTTAAAGAA GATGATGGAA GAATTTGTAC 
     1621 CCCATAGCAA GTCTCTTTCA GATGCACTAA TTTCTCTTCA AATGGTATAT CCTCGAAGGA 
     1681 ATCTTTCAGC TGACCAGTGG AGAAATGCCC AGTTATTGAG CCTCATCAGT GCACCTAGTA 
     1741 CAATGCTTAA TCCAGCACAG TCCGACACTA TGCCTTGTGA ATACCTCTCT TTGGATGCAA 
     1801 TGGAAAAGTG GATTATCTTT GGCTTTATTT TGTGCCATGG GATCCTAAAT ACTGACGCTA 
     1861 CAGCACTGAA CCTTTGGAAA CTAGCTCTTC AAAGTAGCTC TTGCCTCTCT CTCTTTCGGG 
     1921 ATGAAGTTTT CCACATTCAC AAAGCTGCAG AAGACTTATT TGTAAACATA CGAGGCTATA 
     1981 ATAAACGTAT TAATGACATA AGAGAATGCA AGGAGGCAGC CGTGTCACAT GCTGGTTCAA 
     2041 TGCACAGAGA AAGACGCAAG TTTTTAAGAT CTGCACTGAA GGAATTGGCT ACTGTCCTCT 
     2101 CTGATCAACC TGGATTGCTA GGTCCCAAGG CACTTTTTGT TTTTATGGCA TTATCCTTTG 
     2161 CCCGTGATGA AATCATCTGG CTACTTCGTC ATGCAGATAA CATGCCAAAG AAGAGTGCAG 
     2221 ACGACTTTAT AGATAAGCAC ATTGCTGAAT TAATATTTTA CATGGAAGAA CTTAGAGCAC 
     2281 ATGTGAGGAA ATACGGACCT GTAATGCAGA GGTATTACGT GCAGTACCTT TCTGGCTTTG 
     2341 ATGCTGTTGT CCTCAATGAA CTCGTGCAGA ATCTTTCTGT TTGCCCTGAA GATGAATCAA 
     2401 TCATCATGTC CTCTTTTGTT AACACTATGA CTTCCCTAAG TGTAAAACAA GTTGAAGATG 
     2461 GGGAAGTATT TGATTTCAGA GGAATGAGAT TAGATTGGTT TAGGTTACAG GCATATACTA 
     2521 GTGTCTCAAA GGCTTCACTT GGCCTTGCAG ATCACAGAGA ACTTGGAAAG ATGATGAATA 
     2581 CAATAATTTT TCATACAAAA ATGGTAGATT CCTTGGTGGA AATGTTGGTG GAAACATCAG 
     2641 ATCTCTCCAT ATTTTGTTTT TATAGTCGTG CTTTTGAGAA GATGTTTCAA CAGTGTTTGG 
     2701 AGTTACCCTC TCAATCAAGA TACTCAATTG CATTTCCACT ACTTTGCACT CATTTTATGA 
     2761 GTTGCACGCA TGAACTATGT CCAGAAGAGC GACATCATAT TGGAGATCGC AGTCTTTCCT 
     2821 TATGTAATAT GTTCCTAGAT GAAATGGCCA AACAAGCTCG AAATCTCATC ACTGATATTT 
     2881 GCACAGAACA GTGTACCCTT AGTGACCAGT TGCTACCCAA GCATTGTGCC AAAACTATCA 
     2941 GTCAAGCAGT GAATAAGAAA TCAAAAAAGC AGACTGGTAA GAAAGGGGAA CCTGAAAGGG 
     3001 AGAAACCAGG TGTTGAGAGC ATGAGGAAAA ACAGGCTGGT TGTGACCAAC CTTGATAAAT 
     3061 TGCACACTGC ACTTTCTGAG TTATGCTTCT CTATAAATTA TGTACCAAAC ATGGTGGTAT 
     3121 GGGAACATAC CTTTACCCCA CGAGAATATT TGACTTCTCA TCTGGAAATA CGCTTTACCA 
     3181 AGTCAATTGT TGGGATGACT ATGTATAATC AAGCCACACA GGAAATTGCA AAACCTTCAG 
     3241 AACTTCTAAC AAGTGTAAGA GCATACATGA CCGTACTCCA GTCAATAGAA AACTATGTGC 
     3301 AGATTGATAT TACAAGAGTA TTTAATAATG TGCTTCTTCA ACAAACACAA CATTTAGACA 
     3361 GTCATGGAGA GCCAACCATT ACAAGTCTAT ACACAAATTG GTATTTGGAA ACTTTGTTAC 
     3421 GACAAGTCAG CAATGGCCAT ATAGCATATT TTCCTGCAAT GAAAGCGTTT GTGAACTTAC 
     3481 CTACAGAAAA TGAATTAACA TTCAATGCAG AGGAATATTC TGACATATCA GAAATGAGGT 
     3541 CATTATCAGA ACTACTAGGC CCATATGGTA TGAAGTTTCT AAGTGAAAGC CTTATGTGGC 
     3601 ATATTTCATC ACAAGTTGCT GAACTTAAGA AACTTGTGGT GGAGAATGTT GATGTGTTAA 
     3661 CACAAATGAG GACCAGCTTT GACAAACCAG ACCAGATGGC TGCACTGTTT AAAAGATTAT 
     3721 CATCTGTTGA CAGTGTCTTG AAGAGGATGA CAATAATTGG TGTAATTTTA TCCTTCCGAT 
     3781 CATTGGCACA AGAAGCACTT AGAGATGTCT TATCCTACCA CATTCCTTTT CTTGTAAGTT 
     3841 CAATTGAAGA TTTTAAGGAT CACATTCCAA GGGAAACTGA TATGAAGGTT GCAATGAATG 
     3901 TGTATGAGTT ATCATCAGCT GCCGGATTAC CTTGTGAGAT TGATCCTGCA TTGGTCGTAG 
     3961 CTCTTTCTTC ACAAAAATCG GAAAACATTA GTCCAGAAGA AGAGTATAAA ATTGCCTGCC 
     4021 TTCTCATGGT GTTTGTGGCA GTTTCTTTGC CAACACTGGC CAGTAATGTG ATGTCTCAGT 
     4081 ACAGCCCTGC TATAGAAGGG CATTGCAACA ACATACATTG CTTGGCCAAA GCCATCAACC 
     4141 AGATTGCTGC AGCTTTGTTT ACAATTCACA AAGGAAGCAT TGAAGACCGT CTTAAAGAAT 
     4201 TTCTGGCGCT TGCATCCTCC AGTCTACTGA AAATTGGCCA GGAGACAGAT AAAACTACAA 
     4261 CAAGAAATAG AGAATCTGTT TATTTACTGC TAGATATGAT TGTACAAGAA TCTCCATTCC 
     4321 TTACAATGGA TCTTTTGGAA TCTTGTTTTC CTTATGTCTT GCTGAGAAAT GCATACCATG 
     4381 CTGTCTACAA ACAAAGTGTT ACATCTTCTG CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4441 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4501 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4561 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4621 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4681 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4741 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4801 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4861 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4921 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4981 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5041 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5101 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5161 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5221 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5281 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5341 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5401 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5461 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5521 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5581 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5641 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5701 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5761 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5821 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5881 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5941 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6001 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6061 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6121 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6181 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6241 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6301 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6361 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6421 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6481 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6541 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6601 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6661 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6721 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6781 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6841 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6901 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6961 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7021 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7081 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7141 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7201 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7261 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7321 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7381 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7441 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7501 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7681 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7741 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7801 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7861 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7921 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7981 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8041 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8101 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8161 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8221 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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