LOCUS       RC201912                6993 bp    DNA     circular  SYN 
DEFINITION  MTMR8 (Myc-DDK-tagged)-Human myotubularin related protein 8 (MTMR8)
ACCESSION   NM_017677
VERSION     NM_017677.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3150..3241 
                     /label="Myc-DDK"  
     misc_feature    3271..3856 
                     /label="PolyA signal"  
     misc_feature    3328..3347 
                     /label="Restriction Enzyme"  
     misc_feature    4132..4804 
                     /label="ColE1"  
     misc_feature    5234..6028 
                     /label="Kan/Neo"  
     misc_feature    6063..6365 
                     /label="SV40 ori"  
     misc_feature    6451..6928 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3132..3138 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3132 
                     /label="MTMR8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCATATT ACGGTACCCA AGGTAGAAAA CGTGAAATTG GTGGATCGTT 
     1081 ATGTGAGTAA GAAACCAGCT AATGGGATTC TTTATCTTAC TGCAACCCAC CTGATCTATG 
     1141 TGGAGGCTTC AGGTGCAGCC CGGAAAGAAA CATGGATTGC ACTCCATCAC ATTGCCACTG 
     1201 TGGAGAAGTT ACCCATCACT AGCCTGGGTT GTCCCCTGAC CCTCCGCTGC AAGAATTTCC 
     1261 GGGTGGCCCA CTTTGTTTTA GATTCTGACC TTGTGTGCCA TGAGGTTTAT ATTTCACTGC 
     1321 TCAAGCTTTC TCAGCCAGCA TTACCTGAAG ATCTTTATGC TTTTTCTTAT AATCCCAAAT 
     1381 CCTCAAAAGA GATGAGGGAA AGTGGATGGA AACTGATTGA CCCAATATCA GACTTTGGGC 
     1441 GTATGGGAAT ACCCAACAGA AACTGGACCA TAACAGATGC CAACAGAAAC TATGAGATAT 
     1501 GCAGCACCTA CCCTCCTGAA ATAGTGGTTC CTAAATCTGT TACCTTGGGA ACGGTGGTTG 
     1561 GAAGTTCAAA GTTCAGAAGT AAAGAACGTG TCCCTGTGCT CTCCTACCTC TACAAAGAGA 
     1621 ACAATGCTGC CATTTGCCGC TGTAGCCAGC CTCTCTCTGG ATTTTACACT CGCTGTGTAG 
     1681 ATGATGAGCT CTTGTTGGAG GCCATTAGCC AAACAAACCC AGGGAGCCAG TTTATGTATG 
     1741 TTGTAGACAC AAGACCAAAG TTGAATGCCA TGGCCAACCG AGCAGCTGGG AAGGGGTATG 
     1801 AAAATGAAGA CAACTATGCC AACATTCGCT TCAGATTCAT GGGCATTGAG AACATCCATG 
     1861 TAATGCGGAG CAGTCTGCAG AAACTCTTGG AAGTTTGTGA ATTGAAAACT CCAACAATGA 
     1921 GTGAATTTCT TAGCGGCCTG GAGAGCTCAG GGTGGTTAAG ACACATTAAA GCTATTATGG 
     1981 ATGCTGGAAT TTTCATTACA AAGGCAGTGA AGGTAGAAAA GGCCAGTGTC TTAGTCCATT 
     2041 GTTCTGATGG ATGGGACCGC ACAGCACAAG TCTGCTCAGT GGCTAGCATC CTCCTAGATC 
     2101 CATTTTATAG GACATTCAAA GGACTCATGA TCTTGATAGA GAAGGAATGG ATATCCATGG 
     2161 GCCACAAGTT TTCCCAAAGG TGTGGCCACC TCGATGGGGA CTCTAAAGAA GTGTCCCCTA 
     2221 TCTTCACCCA GTTCCTAGAC TGTATCTGGC AATTAATGGA ACAGTTTCCC TGTGCCTTTG 
     2281 AGTTTAATGA AAACTTCCTG CTGGAGATTC ATGACCATGT TTTCTCCTGC CAGTTTGGAA 
     2341 ACTTCCTTGG TAACTGCCAG AAGGATCGGG AAGATCTAAG AGTCTATGAG AAAACACATT 
     2401 CTGTGTGGCC TTTCTTGGTT CAGAGGAAAC CAGACTTCAG GAACCCTCTC TATAAAGGCT 
     2461 TCACTATGTA TGGGGTACTC AATCCTAGTA CTGTGCCCTA CAACATTCAG TTCTGGTGTG 
     2521 GGATGTATAA CCGCTTTGAC AAAGGGCTGC AGCCCAAGCA GAGTATGCTA GAGAGCCTCC 
     2581 TGGAAATTAA GAAACAGAGA GCAATGCTGG AGACAGATGT GCATGAACTA GAAAAGAAAC 
     2641 TAAAAGTCCG TGATGAGCCA CCAGAAGAGA TCTGTACCTG CTCTCAATTA GGAAACATAT 
     2701 TATCCCAGCA TCTGGGAAGT CCTTTGACCA ATCCTCTTGG CTTTATGGGT ATCAATGGAG 
     2761 ACCTGAATAC CCTGATGGAG AATGGCACCC TATCCAGGGA AGGAGGCCTC CGAGCTCAGA 
     2821 TGGATCAAGT AAAAAGCCAG GGTGCAGACC TCCACCATAA CTGTTGTGAG ATTGTGGGCA 
     2881 GCCTTAGGGC CATAAATATC TCTGGGGATG TGGGCATCTC TGAGGCCATG GGCATCTCTG 
     2941 GAGACATGTG CACCTTTGAG GCTACGGGCT TCTCCAAAGA CTTAGGAATC TGTGGGGCCA 
     3001 TGGACATCTC TGAGGCCACT GGCATCTCTG GAAACTTGGG TATTTCTGAG GCCAGGGGTT 
     3061 TCTCTGGGGA CATGGGCATC TTGGGGGACA CAGGCATCTC CAAGGCCAGC ACCAAGGAGG 
     3121 CAGACTACTC CAAGCATCAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3181 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3241 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3301 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3361 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3421 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3481 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3541 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3601 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3661 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3721 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3781 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3841 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3901 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3961 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4021 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4801 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4861 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4921 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4981 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5041 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5101 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5161 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5221 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5281 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5341 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5401 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5461 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5521 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5581 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5641 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5701 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5761 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5821 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5881 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5941 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6001 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6061 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6121 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6181 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6241 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6301 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6361 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6421 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6481 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6541 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6601 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6661 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6721 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6781 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6841 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6901 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6961 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//