LOCUS       RC201874                7848 bp    DNA     circular  SYN 
DEFINITION  MED24 (Myc-DDK-tagged)-Human mediator complex subunit 24 (MED24), transcript variant 1
ACCESSION   NM_014815
VERSION     NM_014815.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4005..4096 
                     /label="Myc-DDK"  
     misc_feature    4126..4711 
                     /label="PolyA signal"  
     misc_feature    4183..4202 
                     /label="Restriction Enzyme"  
     misc_feature    4987..5659 
                     /label="ColE1"  
     misc_feature    6089..6883 
                     /label="Kan/Neo"  
     misc_feature    6918..7220 
                     /label="SV40 ori"  
     misc_feature    7306..7783 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3987..3993 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3987 
                     /label="MED24"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTGGTC AACCTGAAGC AAGCCATTTT GCAAGCCTGG AAGGAGCGCT 
     1081 GGAGTGACTA CCAATGGGCA ATCAACATGA AGAAATTCTT TCCTAAAGGA GCCACCTGGG 
     1141 ATATTCTCAA CCTGGCAGAT GCGTTACTAG AGCAGGCCAT GATTGGACCA TCCCCCAATC 
     1201 CTCTCATCTT GTCCTACCTG AAGTATGCCA TTAGTTCCCA GATGGTGTCC TACTCTTCTG 
     1261 TCCTCACAGC CATCAGTAAG TTTGATGACT TTTCTCGGGA CCTGTGTGTC CAGGCATTGC 
     1321 TGGACATCAT GGACATGTTT TGTGACCGTC TGAGCTGTCA CGGCAAAGCA GAGGAATGCA 
     1381 TCGGACTGTG CCGAGCCCTT CTTAGCGCCC TCCACTGGCT GCTGCGCTGC ACGGCAGCCT 
     1441 CTGCAGAGCG GCTGCGGGAG GGGCTGGAGG CCGGCACTCC AGCCGCTGGG GAGAAGCAGC 
     1501 TTGCCATGTG CCTTCAGCGC CTGGAGAAAA CCCTCAGCAG CACCAAGAAC CGGGCCCTGC 
     1561 TGCACATCGC CAAACTAGAG GAGGCCTCTT CTTGGACTGC CATCGAGCAT TCTCTCTTGA 
     1621 AACTTGGAGA GATCCTGGCC AATCTCAGCA ACCCGCAGCT CCGGAGTCAG GCCGAGCAGT 
     1681 GTGGCACCCT CATTAGGAGC ATCCCCACGA TGCTGTCTGT GCATGCGGAG CAGATGCACA 
     1741 AGACCGGCTT CCCCACTGTC CACGCCGTGA TCCTGCTCGA GGGCACCATG AACCTGACAG 
     1801 GCGAGACGCA GTCCCTGGTG GAGCAGCTGA CGATGGTGAA GCGCATGCAG CATATCCCCA 
     1861 CCCCACTTTT TGTCCTGGAG ATCTGGAAAG CTTGCTTCGT GGGGCTCATT GAGTCTCCCG 
     1921 AGGGTACGGA GGAGCTCAAG TGGACAGCTT TCACTTTCCT CAAGATTCCA CAGGTTTTGG 
     1981 TGAAGTTGAA GAAGTACTCT CATGGAGACA AGGACTTCAC TGAGGATGTC AACTGTGCTT 
     2041 TTGAGTTCCT GCTGAAGCTC ACCCCCTTGT TGGACAAAGC TGACCAGCGC TGCAACTGTG 
     2101 ACTGTACAAA CTTCCTGCTC CAAGAATGTG GCAAGCAGGG GCTTCTGTCT GAGGCCAGCG 
     2161 TCAACAACCT TATGGCTAAG CGCAAAGCAG ACCGAGAGCA CGCACCCCAG CAGAAATCGG 
     2221 GAGAGAATGC CAACATCCAG CCCAACATCC AGCTGATCCT CCGGGCGGAG CCCACTGTCA 
     2281 CAAACATCCT CAAGACGATG GATGCAGACC ACTCTAAGTC ACCGGAGGGA CTGCTGGGAG 
     2341 TCCTGGGCCA CATGCTGTCC GGGAAGAGTC TGGACTTGCT GCTGGCTGCC GCCGCCGCCA 
     2401 CTGGAAAGCT GAAATCCTTC GCCCGGAAAT TCATCAATTT GAATGAATTC ACAACCTATG 
     2461 GCAGCGAAGA AAGCACCAAA CCGGCCTCCG TCCGGGCCCT GCTGTTTGAC ATCTCCTTCC 
     2521 TCATGCTGTG CCATGTGGCC CAGACCTATG GTTCAGAGGT GATTCTGTCC GAGTCGCGCA 
     2581 CAGGAGCTGA GGTGCCCTTC TTCGAGACCT GGATGCAGAC CTGCATGCCT GAGGAGGGCA 
     2641 AGATCCTGAA CCCTGACCAC CCCTGCTTCC GCCCCGACTC CACCAAAGTG GAGTCCCTGG 
     2701 TGGCCCTGCT CAACAACTCC TCGGAGATGA AGCTAGTGCA GATGAAGTGG CATGAGGCCT 
     2761 GTCTCAGCAT CTCAGCCGCC ATCTTGGAAA TCCTCAATGC CTGGGAGAAT GGGGTCCTGG 
     2821 CCTTCGAGTC CATCCAGAAA ATCACTGATA ACATCAAAGG GAAGGTATGC AGTCTGGCGG 
     2881 TGTGTGCTGT GGCTTGGCTT GTGGCCCACG TCCGGATGCT GGGGCTGGAT GAGCGTGAGA 
     2941 AGTCGCTGCA GATGATCCGC CAGCTGGCAG GGCCACTGTT TAGTGAGAAC ACCCTGCAGT 
     3001 TCTACAATGA GAGGGTGGTG ATCATGAACT CGATCCTGGA GCGCATGTGT GCCGACGTGC 
     3061 TGCAGCAGAC AGCCACGCAG ATCAAGTTTC CCTCCACCGG GGTGGACACA ATGCCCTACT 
     3121 GGAACCTGCT GCCCCCCAAG CGGCCCATCA AAGAGGTGCT GACGGACATT TTTGCCAAGG 
     3181 TGCTGGAGAA GGGCTGGGTG GACAGCCGCT CCATCCACAT CTTTGACACC CTGCTGCACA 
     3241 TGGGCGGCGT CTACTGGTTC TGCAACAACC TGATTAAGGA GCTGCTGAAG GAGACGCGGA 
     3301 AGGAGCACAC GCTGCGGGCA GTGGAGCTGC TCTACTCCAT CTTCTGCCTG GACATGCAGC 
     3361 AAGTGACCCT GGTCCTGCTG GGCCACATCC TACCTGGCCT GCTCACTGAC TCCTCCAAGT 
     3421 GGCACAGCCT CATGGACCCC CCGGGCACTG CTCTTGCCAA GCTGGCCGTG TGGTGTGCCC 
     3481 TCAGTTCCTA CTCCTCCCAC AAGGGACAGG CGTCCACCCG CCAGAAGAAG AGACACCGCG 
     3541 AAGACATTGA GGATTATATC AGCCTCTTCC CCCTGGACGA TGTGCAGCCT TCGAAGTTGA 
     3601 TGCGACTGCT GAGCTCTAAT GAGGACGATG CCAACATCCT TTCGAGCCCC ACAGACCGAT 
     3661 CCATGAGCAG CTCCCTCTCA GCCTCTCAGC TCCACACGGT CAACATGCGG GACCCTCTGA 
     3721 ACCGAGTCCT GGCCAACCTG TTCCTGCTCA TCTCCTCCAT CCTGGGGTCT CGCACCGCTG 
     3781 GCCCCCACAC CCAGTTCGTG CAGTGGTTCA TGGAGGAGTG TGTGGACTGC CTGGAGCAGG 
     3841 GTGGCCGTGG CAGCGTCCTG CAGTTCATGC CCTTCACCAC CGTGTCGGAA CTGGTGAAGG 
     3901 TGTCAGCCAT GTCTAGCCCC AAGGTGGTTC TGGCCATCAC GGACCTCAGC CTGCCCCTGG 
     3961 GCCGCCAGGT GGCTGCTAAA GCCATTGCTG CACTCACGCG TACGCGGCCG CTCGAGCAGA 
     4021 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4081 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4141 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4201 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4261 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4321 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4381 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4441 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4501 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4561 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4621 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4681 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4741 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4801 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4861 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4921 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4981 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5041 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5101 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5161 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5221 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5281 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5341 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5401 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5461 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5521 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5581 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5641 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5701 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5761 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5821 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5881 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5941 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6001 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6061 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6121 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6181 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6241 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6301 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6361 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6421 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6481 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6541 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6601 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6661 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6721 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6781 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6841 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6901 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6961 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7021 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7081 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7141 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7261 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7321 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7381 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7441 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7501 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7561 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7621 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7681 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7741 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7801 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//