LOCUS       RC201806                7104 bp    DNA     circular  SYN 
DEFINITION  NUP88 (Myc-DDK-tagged)-Human nucleoporin 88kDa (NUP88)
ACCESSION   NM_002532
VERSION     NM_002532.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3261..3352 
                     /label="Myc-DDK"  
     misc_feature    3382..3967 
                     /label="PolyA signal"  
     misc_feature    3439..3458 
                     /label="Restriction Enzyme"  
     misc_feature    4243..4915 
                     /label="ColE1"  
     misc_feature    5345..6139 
                     /label="Kan/Neo"  
     misc_feature    6174..6476 
                     /label="SV40 ori"  
     misc_feature    6562..7039 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3243..3249 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3243 
                     /label="NUP88"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGCC GAGGGACCGG TGGGCGACGG CGAGCTGTGG CAGACCTGGC 
     1081 TTCCTAACCA CGTCGTGTTC TTGCGGCTCC GGGAGGGACT GAAAAACCAG AGTCCAACCG 
     1141 AAGCTGAGAA ACCAGCTTCT TCGTCGTTGC CTTCGTCGCC GCCGCCGCAG TTGCTGACGA 
     1201 GAAACGTGGT CTTTGGCCTC GGCGGAGAGC TTTTCCTGTG GGACGGAGAA GACAGCTCCT 
     1261 TCTTAGTCGT TCGCCTTCGG GGCCCCAGCG GCGGCGGCGA AGAGCCCGCC CTGTCCCAGT 
     1321 ACCAGAGATT GCTTTGCATA AATCCACCCC TGTTTGAAAT CTATCAAGTC TTGTTAAGCC 
     1381 CAACACAACA TCATGTAGCA CTTATAGGAA TAAAAGGACT TATGGTATTA GAATTACCTA 
     1441 AAAGATGGGG GAAGAATTCT GAATTTGAAG GTGGAAAATC AACAGTGAAT TGTAGTACCA 
     1501 CTCCAGTTGC GGAGAGATTT TTCACCAGTT CCACCTCTCT GACTCTAAAG CATGCTGCAT 
     1561 GGTATCCAAG TGAAATCCTG GATCCCCACG TAGTGCTGTT AACATCAGAC AACGTAATCA 
     1621 GAATTTACTC ACTACGTGAG CCGCAGACAC CCACTAACGT GATAATACTT TCAGAAGCCG 
     1681 AAGAGGAAAG TCTAGTACTC AATAAAGGAA GGGCGTATAC CGCATCTCTA GGAGAGACAG 
     1741 CAGTTGCATT TGACTTTGGG CCATTGGCAG CAGTCCCAAA GACTCTATTT GGACAAAACG 
     1801 GCAAAGATGA AGTAGTGGCA TACCCACTGT ACATCTTATA TGAAAATGGA GAGACTTTCC 
     1861 TGACATACAT CAGTCTGTTA CACAGCCCTG GAAATATTGG AAAGCTGTTG GGTCCATTGC 
     1921 CCATGCATCC TGCGGCTGAA GATAACTATG GTTATGATGC GTGTGCTGTA CTCTGCTTAC 
     1981 CCTGTGTCCC CAATATCTTA GTGATCGCTA CTGAATCAGG AATGCTGTAT CACTGTGTCG 
     2041 TGCTAGAAGG GGAAGAAGAA GATGACCACA CGTCAGAAAA GTCCTGGGAT TCCAGGATTG 
     2101 ACCTCATTCC TTCTCTGTAT GTGTTTGAAT GTGTTGAGTT GGAGCTTGCT TTGAAACTGG 
     2161 CATCTGGAGA GGATGACCCT TTTGATTCTG ACTTTTCTTG TCCAGTCAAA CTTCATAGAG 
     2221 ATCCCAAGTG TCCTTCAAGA TATCACTGTA CTCATGAAGC TGGTGTACAT AGTGTTGGGC 
     2281 TAACTTGGAT TCATAAACTT CACAAATTTC TTGGATCAGA TGAAGAAGAT AAGGATAGTT 
     2341 TACAGGAACT CTCTACAGAA CAGAAATGCT TTGTTGAACA CATCCTTTGT ACGAAGCCAT 
     2401 TGCCCTGCAG GCAGCCAGCT CCAATTCGAG GATTTTGGAT TGTACCTGAC ATTCTGGGAC 
     2461 CCACGATGAT CTGCATCACC AGTACCTATG AATGCCTCAT ATGGCCGTTA TTAAGTACAG 
     2521 TCCATCCAGC GTCTCCTCCC CTGCTTTGTA CTCGAGAAGA TGTTGAAGTG GCAGAGTCTC 
     2581 CCCTCCGTGT TCTGGCTGAA ACCCCAGATT CCTTTGAAAA GCATATTAGA AGCATTTTGC 
     2641 AACGTAGTGT TGCCAATCCA GCATTTTTGA AAGCTTCTGA AAAGGACATA GCCCCTCCTC 
     2701 CTGAAGAATG CCTTCAGCTC CTCAGCAGAG CCACCCAGGT GTTCAGAGAG CAGTACATTC 
     2761 TCAAACAGGA CTTGGCAAAG GAGGAGATTC AGCGGAGGGT CAAATTATTA TGTGACCAAA 
     2821 AAAAGAAACA ACTAGAAGAT CTCAGTTATT GTCGAGAAGA GAGGAAAAGT CTGCGGGAAA 
     2881 TGGCTGAGCG TTTAGCTGAC AAATATGAGG AAGCTAAAGA AAAACAAGAG GATATCATGA 
     2941 ACAGGATGAA AAAACTACTT CACAGTTTTC ACTCTGAGCT CCCAGTTCTC TCTGATAGTG 
     3001 AGCGAGACAT GAAGAAAGAA TTACAGCTGA TACCTGATCA ACTTCGACAT TTGGGCAATG 
     3061 CCATCAAACA GGTTACTATG AAAAAGGATT ATCAACAGCA AAAGATGGAG AAGGTGTTGA 
     3121 GTCTTCCAAA ACCCACCATT ATTCTCAGTG CCTACCAGCG AAAGTGCATT CAGTCCATCC 
     3181 TGAAAGAGGA GGGTGAACAT ATAAGGGAAA TGGTGAAGCA AATCAATGAT ATCCGCAATC 
     3241 ATGTAAACTT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3301 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3361 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3421 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3481 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3541 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3601 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3661 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3721 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3781 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3841 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3901 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3961 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4021 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4081 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4141 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4201 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4261 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4321 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4381 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4441 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4501 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4561 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4621 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4681 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4741 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4801 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4861 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4921 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4981 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5041 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5101 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5161 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5221 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5281 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5341 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5401 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5461 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5521 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5581 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5641 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5701 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5761 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5821 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5881 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5941 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6001 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6061 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6121 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6181 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6241 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6301 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6361 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6421 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6481 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6541 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6601 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6661 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6721 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6781 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6841 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6901 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6961 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7021 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7081 TAACAAAAAT TTAACGCGAA TTTT
//