LOCUS       RC201783                8460 bp    DNA     circular  SYN 
DEFINITION  SPAG5 (Myc-DDK-tagged)-Human sperm associated antigen 5 (SPAG5)
ACCESSION   NM_006461
VERSION     NM_006461.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4617..4708 
                     /label="Myc-DDK"  
     misc_feature    4738..5323 
                     /label="PolyA signal"  
     misc_feature    4795..4814 
                     /label="Restriction Enzyme"  
     misc_feature    5599..6271 
                     /label="ColE1"  
     misc_feature    6701..7495 
                     /label="Kan/Neo"  
     misc_feature    7530..7832 
                     /label="SV40 ori"  
     misc_feature    7918..8395 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4599..4605 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4599 
                     /label="SPAG5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGAGTG AAAAAACTGA GCCTCAGCCT GTCGCCTTCG CCCCAGACGG 
     1081 GAAAACCATC TATGAGAACT CCTCTCCGTG AACTTACCCT GCAGCCCGGT GCCCTCACCA 
     1141 ACTCTGGAAA AAGATCCCCC GCTTGCTCCT CGCTGACCCC ATCACTGTGC AAGCTGGGGC 
     1201 TGCAGGAAGG CAGCAACAAC TCATCTCCAG TGGATTTTGT AAATAACAAG AGGACAGACT 
     1261 TATCTTCAGA ACATTTCAGT CATTCCTCAA AGTGGCTAGA AACTTGTCAG CATGAATCAG 
     1321 ATGAGCAGCC TCTAGATCCA ATTCCCCAAA TTAGCTCTAC TCCTAAAACG TCTGAGGAAG 
     1381 CAGTAGACCC ACTGGGCAAT TATATGGTTA AAACCATCGT CCTTGTACCA TCTCCACTGG 
     1441 GGCAGCAACA AGACATGATA TTTGAGGCCC GTTTAGATAC CATGGCAGAG ACAAACAGCA 
     1501 TATCTTTAAA TGGACCTTTG AGAACAGACG ATCTGGTGAG AGAGGAGGTG GCACCCTGCA 
     1561 TGGGAGACAG GTTTTCAGAA GTTGCTGCTG TATCTGAGAA ACCTATCTTT CAGGAATCTC 
     1621 CGTCCCATCT CTTAGAGGAG TCTCCACCAA ATCCCTGTTC TGAACAACTA CATTGCTCCA 
     1681 AGGAAAGCCT GAGCAGTAGA ACTGAGGCTG TGCGTGAGGA CTTAGTACCT TCTGAAAGTA 
     1741 ACGCCTTCTT GCCTTCCTCT GTTCTCTGGC TTTCCCCTTC AACTGCCTTG GCAGCAGATT 
     1801 TCCGTGTCAA TCATGTGGAC CCAGAGGAGG AAATTGTAGA GCATGGAGCT ATGGAGGAAA 
     1861 GAGAAATGAG GTTTCCCACA CATCCTAAGG AGTCTGAAAC AGAAGATCAA GCACTTGTCT 
     1921 CAAGTGTGGA AGATATTCTG TCCACATGCC TGACACCAAA TCTAGTAGAA ATGGAATCCC 
     1981 AAGAAGCTCC AGGCCCAGCA GTAGAAGATG TTGGTAGGAT TCTTGGCTCT GATACAGAGT 
     2041 CTTGGATGTC CCCACTGGCC TGGCTGGAAA AAGGTGTAAA TACCTCCGTC ATGCTGGAAA 
     2101 ATCTCCGCCA AAGCTTATCC CTTCCCTCGA TGCTTCGGGA TGCTGCAATT GGCACTACCC 
     2161 CTTTCTCTAC TTGCTCGGTG GGGACTTGGT TTACTCCTTC AGCACCACAG GAAAAGAGTA 
     2221 CAAACACATC CCAGACAGGC CTGGTTGGCA CCAAGCACAG TACTTCTGAG ACAGAGCAGC 
     2281 TCCTGTGTGG CCGGCCTCCA GATCTGACTG CCTTGTCTCG ACATGACTTG GAAGATAACC 
     2341 TGCTGAGCTC TCTTGTCATT CTGGAGGTTC TCTCCCGCCA GCTTCGGGAC TGGAAGAGCC 
     2401 AGCTGGCTGT CCCTCACCCA GAAACCCAGG ACAGTAGCAC ACAGACTGAC ACATCTCACA 
     2461 GTGGGATAAC TAATAAACTT CAGCATCTTA AGGAGAGCCA TGAGATGGGA CAGGCCCTAC 
     2521 AGCAGGCCAG AAATGTCATG CAATCATGGG TGCTTATCTC TAAAGAGCTG ATATCCTTGC 
     2581 TTCACCTATC CCTGTTGCAT TTAGAAGAAG ATAAGACTAC TGTGAGTCAG GAGTCTCGGC 
     2641 GTGCAGAAAC ATTGGTCTGT TGCTGTTTTG ATTTGCTGAA GAAATTGAGG GCAAAGCTCC 
     2701 AGAGCCTCAA AGCAGAAAGG GAGGAGGCAA GGCACAGAGA GGAAATGGCT CTCAGAGGCA 
     2761 AGGATGCGGC AGAGATAGTG TTGGAGGCTT TCTGTGCACA CGCCAGCCAG CGCATCAGCC 
     2821 AGCTGGAACA GGACCTAGCA TCCATGCGGG AATTCAGAGG CCTTCTGAAG GATGCCCAGA 
     2881 CCCAACTGGT AGGGCTTCAT GCCAAGCAAG AAGAGCTGGT TCAGCAGACA GTGAGTCTTA 
     2941 CTTCTACCTT GCAACAAGAC TGGAGGTCCA TGCAACTGGA TTATACAACA TGGACAGCTT 
     3001 TGCTGAGTCG GTCCCGACAA CTCACAGAGA AACTCACAGT CAAGAGCCAG CAAGCCCTGC 
     3061 AGGAACGTGA TGTGGCAATT GAGGAAAAGC AGGAGGTTTC TAGGGTGCTG GAACAAGTCT 
     3121 CTGCCCAGTT AGAGGAGTGC AAAGGCCAAA CAGAACAACT GGAGTTGGAA AACAGTCGTC 
     3181 TAGCAACAGA TCTCCGGGCT CAGTTGCAGA TTCTGGCCAA CATGGACAGC CAGCTAAAAG 
     3241 AGCTACAGAG TCAGCATACC CATTGTGCCC AGGACCTGGC TATGAAGGAT GAGTTACTCT 
     3301 GCCAGCTTAC CCAGAGCAAT GAGGAGCAGG CTGCTCAATG GCAAAAGGAA GAGATGGCAC 
     3361 TAAAACACAT GCAGGCAGAA CTGCAGCAGC AACAAGCTGT CCTGGCCAAA GAGGTGCGGG 
     3421 ACCTGAAAGA GACCTTGGAG TTTGCAGACC AGGAGAATCA GGTTGCTCAC CTGGAGCTGG 
     3481 GTCAGGTTGA GTGTCAATTG AAAACCACAC TGGAAGTGCT CCGGGAGCGC AGCTTGCAGT 
     3541 GTGAGAACCT CAAGGACACT GTAGAGAACC TAACGGCTAA ACTGGCCAGC ACCATAGCAG 
     3601 ATAACCAGGA GCAAGATCTG GAGAAAACAC GGCAGTACTC TCAAAAGCTA GGGCTGCTGA 
     3661 CTGAGCAACT ACAGAGCCTG ACTCTCTTTC TACAGACAAA ACTAAAGGAG AAGACTGAAC 
     3721 AAGAGACCCT TCTGCTGAGT ACAGCCTGTC CTCCCACCCA GGAACACCCT CTGCCTAATG 
     3781 ACAGGACCTT CCTGGGAAGC ATCTTGACAG CAGTGGCAGA TGAAGAGCCA GAATCAACTC 
     3841 CTGTGCCCTT GCTTGGAAGT GACAAGAGTG CTTTCACCCG AGTAGCATCA ATGGTTTCCC 
     3901 TTCAGCCCGC AGAGACCCCA GGCATGGAGG AGAGCCTGGC AGAAATGAGT ATTATGACTA 
     3961 CTGAGCTTCA GAGTCTTTGT TCCCTGCTAC AAGAGTCTAA AGAAGAAGCC ATCAGGACTC 
     4021 TGCAGCGAAA AATTTGTGAG CTGCAAGCTA GGCTGCAGGC CCAGGAAGAA CAGCATCAGG 
     4081 AAGTCCAGAA GGCAAAAGAA GCAGACATAG AGAAGCTGAA CCAGGCCTTG TGCTTGCGCT 
     4141 ACAAGAATGA AAAGGAGCTC CAGGAAGTGA TACAGCAGCA GAATGAGAAG ATCCTAGAAC 
     4201 AGATAGACAA GAGTGGCGAG CTCATAAGCC TTAGAGAGGA GGTGACCCAC CTTACCCGCT 
     4261 CACTTCGGCG TGCGGAGACA GAGACCAAAG TGCTCCAGGA GGCCCTGGCA GGCCAGCTGG 
     4321 ACTCCAACTG CCAGCCTATG GCCACCAATT GGATCCAGGA GAAAGTGTGG CTCTCTCAGG 
     4381 AGGTGGACAA ACTGAGAGTG ATGTTCCTGG AGATGAAAAA TGAGAAGGAA AAACTCATGA 
     4441 TCAAGTTCCA GAGCCATAGA AATATCCTAG AGGAGAACCT TCGGCGCTCT GACAAGGAGT 
     4501 TAGAAAAACT AGATGACATT GTTCAGCATA TTTATAAGAC CCTGCTCTCT ATTCCAGAGG 
     4561 TGGTGAGGGG ATGCAAAGAA CTACAGGGAT TGCTGGAATT TCTGAGCACG CGTACGCGGC 
     4621 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4681 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4741 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4801 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4861 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4921 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4981 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5041 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5101 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5161 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5221 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5281 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5341 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5401 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5461 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5521 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5581 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5641 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5701 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5761 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5821 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5881 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5941 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6001 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6061 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6121 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6181 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6241 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6301 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6361 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6421 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6481 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6541 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6601 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6661 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6721 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6781 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6841 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6901 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6961 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7021 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7081 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7141 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7201 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7261 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7321 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7381 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7441 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7501 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7561 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7621 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7681 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7861 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7921 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7981 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8041 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8101 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8161 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8221 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8281 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8341 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8401 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//