LOCUS       RC201775                6921 bp    DNA     circular  SYN 
DEFINITION  GNPAT (Myc-DDK-tagged)-Human glyceronephosphate O-acyltransferase (GNPAT)
ACCESSION   NM_014236
VERSION     NM_014236.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3078..3169 
                     /label="Myc-DDK"  
     misc_feature    3199..3784 
                     /label="PolyA signal"  
     misc_feature    3256..3275 
                     /label="Restriction Enzyme"  
     misc_feature    4060..4732 
                     /label="ColE1"  
     misc_feature    5162..5956 
                     /label="Kan/Neo"  
     misc_feature    5991..6293 
                     /label="SV40 ori"  
     misc_feature    6379..6856 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3060..3066 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3060 
                     /label="GNPAT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCTTCC AGTTCATCTA ACTCTTATTT CTCCGTTGGC CCAACCAGTC 
     1081 CCAGCGCTGT CGTGCTCCTC TACTCGAAGG AGCTCAAAAA GTGGGATGAG TTTGAAGATA 
     1141 TTTTAGAAGA GAGGAGGCAT GTCAGTGACT TGAAATTTGC AATGAAATGC TACACACCTC 
     1201 TTGTCTATAA GGGAATTACT CCATGTAAAC CAATTGATAT TAAATGTAGT GTTCTCAATT 
     1261 CTGAGGAGAT TCATTATGTC ATTAAACAGC TTTCCAAGGA ATCCCTTCAA TCTGTGGATG 
     1321 TCCTCCGAGA GGAAGTGAGT GAGATCTTAG ATGAAATGAG TCACAAACTG CGTCTTGGAG 
     1381 CCATTCGGTT TTGTGCCTTC ACCCTGAGCA AAGTATTTAA ACAAATTTTC TCGAAGGTGT 
     1441 GTGTAAATGA AGAAGGTATT CAGAAACTAC AAAGAGCCAT CCAGGAGCAT CCTGTTGTTC 
     1501 TGCTGCCTAG TCATCGAAGT TACATTGACT TCCTCATGTT GTCTTTTCTT CTATACAATT 
     1561 ATGATTTGCC TGTGCCAGTT ATAGCAGCAG GAATGGACTT CCTGGGAATG AAAATGGTTG 
     1621 GTGAGCTGCT ACGAATGTCG GGTGCCTTTT TCATGCGGCG TACCTTTGGT GGCAATAAAC 
     1681 TCTACTGGGC TGTATTCTCT GAATATGTAA AAACTATGTT ACGGAATGGT TATGCTCCTG 
     1741 TTGAATTTTT CCTCGAAGGG ACAAGAAGCC GCTCTGCCAA GACATTGACT CCTAAATTTG 
     1801 GTCTTCTGAA TATTGTGATG GAGCCATTTT TTAAAAGAGA AGTTTTTGAT ACCTACCTTG 
     1861 TCCCAATTAG TATCAGTTAT GATAAGATCT TGGAAGAAAC TCTTTATGTG TATGAGCTTC 
     1921 TAGGGGTTCC TAAACCAAAA GAATCTACAA CTGGGTTGCT GAAAGCCAGA AAGATTCTCT 
     1981 CTGAAAATTT TGGAAGCATC CATGTGTACT TTGGAGATCC TGTGTCACTT CGATCTTTGG 
     2041 CAGCTGGGAG GATGAGTCGG AGCTCATATA ACTTGGTTCC AAGATACATT CCTCAGAAAC 
     2101 AGTCTGAGGA CATGCATGCC TTTGTCACTG AAGTTGCCTA CAAAATGGAG CTTCTGCAAA 
     2161 TTGAAAACAT GGTTTTGAGC CCCTGGACCC TAATAGTTGC TGTTCTGCTT CAGAACCGGC 
     2221 CATCCATGGA CTTTGATGCT CTGGTGGAAA AGACTTTATG GCTAAAAGGC TTAACCCAGG 
     2281 CATTTGGAGG GTTTCTCATT TGGCCTGATA ATAAACCTGC TGAAGAAGTT GTCCCGGCCA 
     2341 GCATTCTTCT GCATTCCAAC ATTGCCAGCC TTGTCAAAGA CCAGGTGATT CTGAAAGTGG 
     2401 ACTCCGGAGA CTCGGAAGTG GTCGATGGGC TTATGCTCCA GCACATCACT CTCCTCATGT 
     2461 GCTCAGCTTA TAGGAACCAG CTGCTCAACA TTTTTGTGCG CCCATCCTTA GTAGCAGTAG 
     2521 CATTGCAGAT GACACCAGGG TTCAGGAAAG AGGATGTCTA CAGTTGCTTT CGCTTCCTAC 
     2581 GTGATGTTTT TGCAGATGAG TTCATCTTCC TTCCAGGAAA CACACTAAAG GACTTTGAAG 
     2641 AAGGCTGTTA CCTGCTTTGT AAAAGTGAAG CCATACAAGT GACTACGAAA GACATCCTAG 
     2701 TTACAGAGAA AGGAAATACT GTGTTAGAAT TTTTAGTAGG ACTCTTTAAA CCTTTTGTGG 
     2761 AAAGCTATCA GATAATTTGC AAGCACCTTT TGAGTGAAGA AGAGGACCAC TTCAGTGAGG 
     2821 AACAGTACTT GGCTGCAGTC AGAAAATTCA CAAGTCAGCT TCTCGATCAA GGTACCTCTC 
     2881 AATGTTATGA TGTATTATCT TCTGATGTGC AGAAAAACGC CTTAGCAGCC TGTGTGAGGC 
     2941 TCGGAGTAGT GGAGAAGAAG AAGATAAATA ATAACTGTAT ATTTAATGTG AATGAACCTG 
     3001 CCACAACCAA ATTAGAAGAA ATGCTTGGTT GTAAGACACC AATAGGAAAA CCAGCCACTG 
     3061 CAAAACTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3121 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3181 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3241 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3301 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3361 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3421 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3481 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3541 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3601 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3661 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3721 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3781 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3841 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3901 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     3961 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4021 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4081 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4141 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4201 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4261 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4321 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4381 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4441 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4501 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4561 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4621 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4681 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4741 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4801 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4861 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4921 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     4981 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5041 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5101 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5161 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5221 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5281 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5341 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5401 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5461 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5521 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5581 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5641 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5701 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5761 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5821 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5881 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     5941 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6001 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6061 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6121 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6181 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6241 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6301 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6361 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6421 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6481 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6541 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6601 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6661 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6721 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6781 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6841 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6901 CAAAAATTTA ACGCGAATTT T
//