LOCUS       RC201758                7341 bp    DNA     circular  SYN 
DEFINITION  DDX23 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23)
ACCESSION   NM_004818
VERSION     NM_004818.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3498..3589 
                     /label="Myc-DDK"  
     misc_feature    3619..4204 
                     /label="PolyA signal"  
     misc_feature    3676..3695 
                     /label="Restriction Enzyme"  
     misc_feature    4480..5152 
                     /label="ColE1"  
     misc_feature    5582..6376 
                     /label="Kan/Neo"  
     misc_feature    6411..6713 
                     /label="SV40 ori"  
     misc_feature    6799..7276 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3480..3486 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3480 
                     /label="DDX23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGAGAG CTGGCTGACA AAAAGGACCG TGATGCATCA CCTTCCAAGG 
     1081 AGGAAAGGAA GCGATCACGG ACTCCTGACA GAGAGCGGGA TAGAGACCGG GACCGGAAGT 
     1141 CTTCCCCATC TAAAGATAGA AAGCGGCATC GTTCAAGGGA TAGACGTCGA GGAGGCAGCC 
     1201 GTTCTCGCTC TCGTTCCCGT TCCAAATCTG CAGAAAGAGA ACGACGGCAC AAAGAACGAG 
     1261 AACGAGATAA GGAGCGGGAT CGGAATAAGA AGGACCGAGA TCGAGACAAG GATGGGCACA 
     1321 GACGGGACAA GGACCGTAAA CGATCCAGCT TATCTCCTGG TCGAGGAAAA GACTTTAAAT 
     1381 CTCGGAAGGA CAGAGACTCT AAGAAGGATG AAGAGGATGA ACATGGTGAT AAGAAGCCTA 
     1441 AGGCCCAGCC ATTATCCCTG GAGGAGCTTC TGGCCAAGAA AAAGGCTGAG GAAGAAGCTG 
     1501 AGGCTAAGCC CAAGTTCCTC TCTAAAGCAG AACGAGAGGC TGAAGCTCTA AAGCGACGGC 
     1561 AGCAGGAGGT GGAAGAGCGG CAGAGGATGC TTGAAGAAGA GAGGAAGAAA AGGAAACAGT 
     1621 TCCAAGACTT GGGCAGGAAG ATGTTGGAAG ATCCTCAGGA ACGGGAACGT CGGGAACGCA 
     1681 GGGAGAGGAT GGAACGGGAG ACCAATGGAA ATGAGGATGA GGAAGGGCGG CAGAAGATCC 
     1741 GGGAAGAGAA GGATAAGAGC AAGGAACTGC ATGCCATTAA GGAGCGTTAC CTGGGTGGCA 
     1801 TCAAAAAGCG GCGCCGAACG AGACATCTCA ATGACCGGAA ATTTGTTTTT GAGTGGGATG 
     1861 CATCTGAGGA CACATCCATT GACTACAACC CCCTGTACAA AGAACGGCAC CAGGTGCAGT 
     1921 TGTTAGGGCG AGGCTTCATT GCAGGCATTG ACCTCAAGCA GCAGAAGCGA GAGCAGTCAC 
     1981 GTTTCTATGG AGACCTAATG GAGAAGAGGC GAACCCTGGA AGAAAAGGAG CAGGAGGAGG 
     2041 CAAGACTCCG CAAACTTCGT AAGAAGGAAG CCAAGCAGCG CTGGGATGAT CGTCATTGGT 
     2101 CTCAGAAAAA GTTAGATGAG ATGACGGACA GGGACTGGCG GATCTTCCGT GAGGACTACA 
     2161 GCATCACCAC CAAAGGTGGC AAGATCCCCA ATCCCATCCG ATCCTGGAAA GACTCTTCTC 
     2221 TGCCCCCACA CATCTTGGAG GTCATTGATA AGTGTGGCTA CAAGGAACCA ACACCTATAC 
     2281 AGCGTCAGGC AATTCCCATT GGGCTACAGA ATCGTGACAT CATTGGTGTG GCTGAGACTG 
     2341 GCAGTGGCAA GACAGCAGCC TTCCTCATCC CTCTGCTGGT CTGGATCACC ACACTTCCCA 
     2401 AAATTGACAG GATCGAAGAG TCAGACCAAG GCCCTTATGC CATCATCCTG GCTCCCACCC 
     2461 GTGAGTTGGC TCAACAGATT GAGGAAGAGA CCATCAAGTT TGGGAAACCG CTAGGTATCC 
     2521 GCACTGTGGC TGTCATTGGT GGCATCTCCA GAGAAGACCA GGGCTTCAGG CTGCGCATGG 
     2581 GTTGTGAGAT TGTGATTGCT ACCCCTGGGC GTTTGATTGA TGTGCTGGAG AACCGCTACC 
     2641 TGGTGCTGAG CCGCTGTACC TATGTGGTTC TGGATGAGGC AGATAGGATG ATTGACATGG 
     2701 GCTTTGAGCC AGATGTCCAG AAGATCCTGG AGCACATGCC TGTCAGCAAC CAGAAGCCAG 
     2761 ACACGGATGA GGCTGAGGAC CCTGAGAAGA TGCTGGCCAA CTTTGAGTCG GGAAAACATA 
     2821 AGTACCGCCA AACAGTCATG TTCACGGCCA CCATGCCCCC AGCGGTGGAG CGTCTGGCCA 
     2881 GGAGCTATCT TCGGCGACCT GCTGTGGTGT ACATTGGCTC CGCAGGCAAG CCCCATGAGC 
     2941 GTGTGGAACA GAAGGTCTTC CTCATGTCAG AGTCAGAAAA GAGGAAAAAG CTGCTGGCAA 
     3001 TCTTGGAGCA AGGCTTTGAC CCACCCATCA TTATTTTTGT CAACCAGAAG AAGGGCTGCG 
     3061 ACGTGTTGGC CAAATCCCTG GAGAAGATGG GGTACAATGC TTGCACACTG CACGGTGGAA 
     3121 AAGGCCAGGA GCAGCGAGAG TTTGCGTTGT CCAACCTCAA GGCTGGGGCC AAGGATATTT 
     3181 TGGTGGCTAC AGATGTGGCT GGTCGTGGTA TTGACATCCA AGATGTGTCT ATGGTTGTCA 
     3241 ACTATGATAT GGCCAAAAAT ATTGAAGATT ACATCCACCG CATTGGCCGC ACGGGACGAG 
     3301 CAGGCAAGAG TGGGGTGGCC ATCACCTTCC TCACAAAAGA GGACTCTGCT GTGTTCTACG 
     3361 AGCTGAAGCA AGCTATCCTG GAAAGCCCAG TGTCTTCCTG TCCCCCCGAA CTAGCCAACC 
     3421 ACCCAGATGC CCAGCATAAG CCAGGCACCA TCCTCACCAA GAAGCGCCGG GAAGAGACCA 
     3481 TCTTTGCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3541 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3601 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3661 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3721 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3781 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3841 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3901 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3961 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4021 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4081 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4141 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4201 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4261 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4321 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4381 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4441 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4501 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4561 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4621 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4681 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4741 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4801 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4861 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4921 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4981 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5041 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5101 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5161 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5221 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5281 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5341 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5401 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5461 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5521 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5581 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5641 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5701 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5761 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5821 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5881 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5941 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6001 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6061 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6121 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6181 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6241 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6301 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6361 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6421 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6481 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6541 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6601 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6661 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6721 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6781 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6841 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6901 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6961 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7021 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7081 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7141 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7201 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7261 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7321 CAAAAATTTA ACGCGAATTT T
//