LOCUS       RC201554                6702 bp    DNA     circular  SYN 
DEFINITION  RPN1 (Myc-DDK-tagged)-Human ribophorin I (RPN1)
ACCESSION   NM_002950
VERSION     NM_002950.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2859..2950 
                     /label="Myc-DDK"  
     misc_feature    2980..3565 
                     /label="PolyA signal"  
     misc_feature    3037..3056 
                     /label="Restriction Enzyme"  
     misc_feature    3841..4513 
                     /label="ColE1"  
     misc_feature    4943..5737 
                     /label="Kan/Neo"  
     misc_feature    5772..6074 
                     /label="SV40 ori"  
     misc_feature    6160..6637 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2841..2847 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2841 
                     /label="RPN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGCCA GCCGCCGGCT TGTTTCTGCT CCTGTTGCTT GGGACTTGGG 
     1081 CCCCGGCGCC GGGCAGCGCC TCCTCCGAGG CACCGCCGCT GATCAATGAG GACGTGAAGC 
     1141 GCACAGTGGA CCTAAGCAGC CACCTGGCTA AGGTGACGGC CGAGGTGGTC CTGGCGCACC 
     1201 TGGGCGGCGG CTCCACGTCC CGAGCTACCT CTTTCCTGCT GGCTTTGGAG CCTGAGCTCG 
     1261 AGGCCCGGCT GGCGCACCTG GGCGTGCAGG TAAAGGGAGA AGATGAGGAA GAGAACAATT 
     1321 TGGAAGTACG TGAAACCAAA ATTAAGGGTA AAAGTGGGAG ATTCTTCACA GTCAAGCTCC 
     1381 CAGTTGCTCT TGATCCTGGG GCCAAGATTT CAGTCATTGT GGAAACAGTC TACACCCATG 
     1441 TGCTTCATCC ATATCCAACC CAGATCACCC AGTCAGAGAA ACAGTTTGTG GTGTTTGAGG 
     1501 GGAACCATTA TTTCTACTCT CCCTATCCAA CGAAGACACA AACCATGCGT GTGAAGCTTG 
     1561 CCTCTCGAAA TGTGGAGAGC TACACCAAGC TGGGGAACCC CACGCGCTCT GAGGACCTAC 
     1621 TGGATTATGG GCCTTTCAGA GATGTGCCTG CCTATAGTCA GGATACTTTT AAAGTACATT 
     1681 ATGAGAACAA CAGCCCTTTC CTGACCATCA CCAGCATGAC CCGAGTCATT GAAGTCTCTC 
     1741 ACTGGGGTAA TATTGCTGTG GAAGAAAATG TGGACTTAAA GCACACAGGA GCTGTGCTTA 
     1801 AGGGGCCTTT CTCACGCTAT GATTACCAGA GACAGCCAGA TAGTGGAATA TCCTCCATCC 
     1861 GTTCTTTTAA GACCATCCTT CCTGCTGCTG CCCAGGATGT TTATTACCGG GATGAGATTG 
     1921 GCAATGTTTC TACCAGCCAC CTCCTTATTT TGGATGACTC TGTAGAGATG GAAATCCGGC 
     1981 CTCGCTTCCC TCTCTTTGGC GGGTGGAAGA CCCATTACAT CGTTGGCTAC AACCTCCCAA 
     2041 GCTATGAGTA CCTCTATAAT TTGGGTGACC AGTATGCACT GAAGATGAGG TTTGTGGACC 
     2101 ATGTGTTTGA TGAACAAGTG ATAGATTCTC TGACTGTGAA GATCATCCTG CCTGAAGGAG 
     2161 CCAAGAACAT TGAAATTGAT AGTCCCTATG AAATCAGCCG TGCCCCAGAT GAGCTGCACT 
     2221 ACACCTATCT GGATACATTT GGCCGCCCTG TGATTGTTGC CTACAAGAAA AATCTGGTAG 
     2281 AACAGCACAT TCAGGACATT GTGGTCCACT ACACGTTCAA CAAGGTGCTC ATGCTGCAGG 
     2341 AGCCCCTGCT GGTGGTGGCG GCCTTCTACA TCCTGTTCTT CACCGTTATC ATCTATGTTC 
     2401 GGCTGGACTT CTCCATCACC AAGGATCCAG CCGCAGAAGC CAGGATGAAG GTAGCCTGCA 
     2461 TCACAGAGCA GGTCTTGACC CTGGTCAACA AGAGAATAGG CCTTTACCGT CACTTTGACG 
     2521 AGACCGTCAA TAGGTACAAG CAATCCCGGG ACATCTCCAC CCTCAACAGT GGCAAGAAGA 
     2581 GCCTGGAGAC TGAACACAAG GCCTTGACCA GTGAGATTGC ACTGCTGCAG TCCAGGCTGA 
     2641 AGACAGAGGG CTCTGATCTG TGCGACAGAG TGAGCGAAAT GCAGAAGCTG GATGCACAGG 
     2701 TCAAGGAGCT GGTGCTGAAG TCGGCGGTGG AGGCTGAGCG CCTGGTGGCT GGCAAGCTCA 
     2761 AGAAAGACAC GTACATTGAG AATGAGAAGC TCATCTCAGG AAAGCGCCAG GAGCTGGTCA 
     2821 CCAAGATCGA CCACATCCTG GATGCCCTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2881 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     2941 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3001 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3061 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3121 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3181 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3241 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3301 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3361 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3421 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3481 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3541 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3601 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3661 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3721 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3781 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3841 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3901 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     3961 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4021 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4081 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4141 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4201 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4261 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4321 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4381 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4441 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4501 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4561 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4621 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4681 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4741 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4801 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4861 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4921 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     4981 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5041 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5101 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5161 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5221 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5281 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5341 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5401 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5461 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5521 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5581 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5641 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5701 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5761 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5821 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5881 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     5941 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6001 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6061 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6121 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6181 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6241 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6301 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6361 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6421 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6481 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6541 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6601 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6661 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//