LOCUS       RC201404                7794 bp    DNA     circular  SYN 
DEFINITION  DIS3L (Myc-DDK-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae)-like (DIS3L), transcript variant 2
ACCESSION   NM_133375
VERSION     NM_133375.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4042 
                     /label="Myc-DDK"  
     misc_feature    4072..4657 
                     /label="PolyA signal"  
     misc_feature    4129..4148 
                     /label="Restriction Enzyme"  
     misc_feature    4933..5605 
                     /label="ColE1"  
     misc_feature    6035..6829 
                     /label="Kan/Neo"  
     misc_feature    6864..7166 
                     /label="SV40 ori"  
     misc_feature    7252..7729 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3933..3939 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3933 
                     /label="DIS3L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGACAGCT TGTCAAGCTG TGCAGCATCA AAGAGGCAGG AGACAGTATA 
     1081 ACAAACTGCG AAACCTGCTG AAGGATGCGC GTCATGATTG CATTCTCTTT GCTAATGAAT 
     1141 TCCAGCAATG CTGCTATCTG CCACGGGAAA GAGGAGAGTC CATGGAGAAG TGGCAGACCA 
     1201 GGAGCATATA CAACGCAGCT GTTTGGTACT ATCATCACTG CCAGGACAGG ATGCCAATTG 
     1261 TTATGGTGAC AGAAGATGAA GAGGCAATTC AGCAGTATGG AAGTGAAACA GAAGGAGTAT 
     1321 TCGTGATTAC TTTCAAGAAT TACCTGGACA ATTTCTGGCC TGATTTAAAA GCTGCCCACG 
     1381 AGCTTTGTGA TTCTATCCTT CAGTCTCGAC GGGAGAGAGA GAATGAGAGT CAGGAGAGCC 
     1441 ATGGGAAGGA GTACCCAGAA CATCTTCCCC TGGAAGTGTT AGAAGCTGGG ATTAAATCTG 
     1501 GACGCTATAT CCAGGGAATT CTGAATGTCA ACAAACACAG AGCCCAAATA GAAGCTTTTG 
     1561 TTCGACTTCA AGGAGCCAGC AGTAAAGATT CAGATTTAGT CAGTGACATC CTAATCCACG 
     1621 GGATGAAGGC TCGAAACCGC TCAATTCATG GAGATGTGGT AGTTGTGGAG CTGCTTCCTA 
     1681 AAAATGAATG GAAAGGAAGA ACCGTAGCCC TGTGTGAGAA TGACTGTGAC GACAAGGCTT 
     1741 CGGGCGAGTC CCCAAGTGAG CCCATGCCTA CAGGTCGAGT GGTGGGCATA CTTCAGAAGA 
     1801 ACTGGCGGGA TTATGTGGTG ACATTTCCGT CCAAAGAAGA GGTCCAATCT CAGGGCAAAA 
     1861 ATGCTCAGAA AATCCTGGTT ACACCTTGGG ATTACAGAAT TCCCAAAATT CGAATTAGCA 
     1921 CTCAGCAAGC AGAAACCCTC CAGGACTTCA GGGTGGTCGT GCGCATCGAT TCCTGGGAGT 
     1981 CAACATCTGT GTATCCAAAC GGACATTTTG TGCGTGTTTT AGGAAGAATC GGAGATCTGG 
     2041 AAGGGGAAAT TGCAACCATC CTGGTGGAAA ACAGTATTTC AGTTATTCCT TTCTCAGAAG 
     2101 CTCAGATGTG TGAGATGCCA GTAAACACAC CAGAAAGTCC CTGGAAGGTG AGTCCTGAAG 
     2161 AGGAACAAAA ACGTAAAGAC TTGAGGAAAA GCCATCTCGT ATTCAGCATT GACCCCAAAG 
     2221 GTTGTGAAGA TGTGGATGAC ACACTCTCAG TCAGAACCTT AAATAATGGC AACCTGGAAC 
     2281 TTGGGGTCCA CATCGCAGAT GTAACACACT TTGTGGCACC AAATTCTTAC ATTGATATTG 
     2341 AAGCTAGAAC AAGGGCCACC ACTTATTATC TAGCAGATCG TCGCTATGAC ATGCTGCCTT 
     2401 CCGTCCTCAG TGCAGATTTG TGTTCCCTTC TGGGAGGCGT TGATAGGTAT GCTGTAAGCA 
     2461 TCATGTGGGA ACTGGATAAA GCCTCTTATG AAATTAAGAA AGTGTGGTAT GGCAGAACCA 
     2521 TTATTCGATC AGCATACAAA CTGTTCTATG AAGCAGCCCA AGAACTACTG GATGGAAACT 
     2581 TAAGCGTTGT TGATGATATT CCAGAATTCA AAGACTTGGA TGAGAAGAGC AGACAAGCCA 
     2641 AGCTGGAGGA GTTGGTGTGG GCAATTGGAA AGCTGACCGA CATAGCTCGC CATGTCAGAG 
     2701 CTAAACGAGA CGGATGTGGT GCCCTGGAAC TGGAAGGGGT AGAGGTTTGC GTACAGCTAG 
     2761 ATGACAAAAA GAACATTCAC GACCTCATCC CCAAGCAGCC CCTGGAAGTC CACGAGACAG 
     2821 TGGCTGAATG CATGATCCTG GCCAACCACT GGGTCGCCAA AAAGATCTGG GAGAGCTTCC 
     2881 CTCATCAGGC CTTGCTGCGC CAGCACCCTC CTCCACACCA GGAGTTCTTT TCAGAACTCC 
     2941 GGGAATGTGC TAAAGCCAAA GGCTTCTTCA TAGATACACG GTCCAATAAA ACACTGGCTG 
     3001 ATTCTCTGGA TAATGCGAAC GACCCCCACG ATCCCATTGT GAACAGGCTA CTGCGCTCCA 
     3061 TGGCCACGCA GGCCATGTCG AATGCTCTGT ACTTCTCCAC CGGATCCTGT GCGGAGGAGG 
     3121 AGTTCCATCA TTACGGTCTT GCATTAGATA AATATACCCA CTTTACTTCT CCAATAAGAA 
     3181 GATATTCAGA TATTGTAGTA CACCGCTTGT TAATGGCAGC CATTTCAAAA GATAAGAAAA 
     3241 TGGAAATTAA GGGAAATCTG TTCAGCAACA AAGATCTTGA GGAATTATGC AGACATATCA 
     3301 ACAACAGAAA CCAAGCAGCA CAGCATTCTC AGAAGCAGTC TACTGAGCTC TTCCAGTGCA 
     3361 TGTACTTCAA AGACAAAGAC CCTGCCACCG AGGAGCGTTG CATATCTGAC GGAGTTATTT 
     3421 ATTCAATTAG AACAAATGGT GTGCTTCTAT TTATACCAAG GTTTGGGATT AAAGGTGCTG 
     3481 CTTATCTAAA AAATAAAGAT GGTTTAGTCA TCTCATGTGG CCCAGATAGC TGTTCTGAAT 
     3541 GGAAACCAGG ATCCCTTCAA CGATTTCAAA ACAAAATTAC CTCTACTACA ACAGATGGGG 
     3601 AATCTGTTAC GTTCCATTTG TTTGACCATG TAACCGTAAG AATATCCATA CAGGCCTCAC 
     3661 GTTGCCATTC TGATACAATC AGACTTGAAA TAATTAGTAA CAAACCATAC AAGATACCAA 
     3721 ATACAGAACT TATTCATCAG AGTTCCCCCT TGCTGAAGAG TGAGTTAGTG AAAGAAGTAA 
     3781 CTAAATCTGT GGAAGAAGCT CAGCTTGCCC AAGAAGTCAA AGTAAACATC ATTCAGGAGG 
     3841 AATATCAAGA ATATCGCCAA ACAAAGGGAA GGAGCCTATA CACACTTCTA GAGGAGATAC 
     3901 GGGACCTAGC TCTCCTGGAT GTTTCAAACA ATTATGGAAT AACGCGTACG CGGCCGCTCG 
     3961 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4021 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4081 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4141 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4201 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4261 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4321 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4381 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4441 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4501 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4561 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4621 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4681 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4741 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4801 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5461 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5521 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5581 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5641 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5701 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5761 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5821 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5881 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5941 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6001 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6061 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6121 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6181 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6241 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6301 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6361 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6421 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6481 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6541 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6601 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6661 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6721 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6781 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6841 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6901 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6961 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7021 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7141 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7201 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7261 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7321 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7381 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7441 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7501 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7561 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7621 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7681 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7741 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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