LOCUS       RC201380                8193 bp    DNA     circular  SYN 
DEFINITION  ESYT1 (Myc-DDK-tagged)-Human extended synaptotagmin-like protein 1 (ESYT1), transcript variant 2
ACCESSION   NM_015292
VERSION     NM_015292.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4350..4441 
                     /label="Myc-DDK"  
     misc_feature    4471..5056 
                     /label="PolyA signal"  
     misc_feature    4528..4547 
                     /label="Restriction Enzyme"  
     misc_feature    5332..6004 
                     /label="ColE1"  
     misc_feature    6434..7228 
                     /label="Kan/Neo"  
     misc_feature    7263..7565 
                     /label="SV40 ori"  
     misc_feature    7651..8128 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4332..4338 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4332 
                     /label="ESYT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGATCT CCAGGAGAGG GCCCCAGCCC CAGCCCCATG GACCAGCCCT 
     1081 CTGCTCCCTC CGACCCCACT GACCAGCCCC CCGCTGCTCA CGCAAAGCCA GACCCAGGTT 
     1141 CTGGGGGCCA ACCTGCTGGC CCTGGCGCGG CGGGTGAGGC CCTGGCGGTG CTGACTTCAT 
     1201 TCGGGAGGCG GTTGCTGGTG CTGATACCTG TGTATTTGGC CGGGGCAGTG GGACTCAGCG 
     1261 TGGGTTTCGT GCTCTTCGGC CTCGCCCTCT ACCTGGGCTG GCGCCGGGTC CGCGACGAGA 
     1321 AAGAACGGAG CCTTCGAGCA GCGAGGCAGC TACTGGACGA CGAGGAGCAG CTCACTGCGA 
     1381 AAACTCTCTA TATGAGTCAT CGAGAGCTAC CTGCCTGGGT CAGCTTCCCA GACGTGGAAA 
     1441 AGGCTGAATG GCTCAATAAG ATTGTGGCCC AGGTCTGGCC CTTCCTGGGC CAGTATATGG 
     1501 AGAAGCTTCT GGCTGAAACT GTGGCTCCGG CTGTTAGGGG ATCTAACCCC CATCTGCAAA 
     1561 CATTTACATT TACACGAGTG GAACTGGGTG AAAAGCCATT GCGCATCATT GGAGTCAAGG 
     1621 TTCACCCAGG TCAGAGAAAA GAGCAGATCC TGCTGGACTT GAACATCAGC TATGTAGGTG 
     1681 ATGTGCAGAT TGATGTGGAA GTGAAGAAAT ATTTTTGCAA AGCAGGAGTC AAGGGCATGC 
     1741 AGCTACATGG CGTTTTGCGG GTGATACTGG AGCCACTCAT TGGGGACCTT CCCTTCGTGG 
     1801 GGGCTGTGTC AATGTTCTTC ATCCGACGCC CGACCCTAGA CATCAACTGG ACAGGGATGA 
     1861 CCAACCTGCT GGATATCCCA GGACTTAGCT CACTCTCTGA CACCATGATC ATGGACTCCA 
     1921 TTGCTGCCTT CCTCGTGTTG CCCAACCGAT TACTGGTGCC CCTTGTGCCT GACCTTCAAG 
     1981 ATGTGGCTCA GTTGCGTTCC CCTCTGCCCA GGGGCATTAT TCGAATTCAC CTGCTGGCTG 
     2041 CTCGAGGGCT GAGTTCCAAG GACAAATATG TGAAGGGCCT GATTGAGGGC AAGTCAGACC 
     2101 CATATGCACT TGTGCGTTTG GGTACCCAGA CATTCTGCAG TCGTGTCATT GATGAAGAAC 
     2161 TCAACCCACA GTGGGGAGAG ACTTATGAGG TGATGGTACA CGAGGTCCCA GGGCAGGAGA 
     2221 TTGAAGTGGA GGTGTTCGAC AAGGATCCAG ATAAAGATGA CTTTCTGGGC AGAATGAAGC 
     2281 TGGATGTAGG GAAGGTGTTA CAGGCTAGCG TTCTGGATGA TTGGTTCCCT CTACAAGGTG 
     2341 GGCAAGGCCA AGTTCACTTG AGGCTAGAAT GGCTGTCACT TTTGTCAGAT GCAGAGAAAC 
     2401 TGGAGCAGGT TCTACAGTGG AATTGGGGAG TCTCCTCTCG ACCAGATCCC CCGTCAGCTG 
     2461 CCATCTTAGT TGTCTACCTG GATCGGGCCC AGGATCTTCC TCTGAAGAAG GGGAACAAGG 
     2521 AACCCAACCC TATGGTACAA CTGTCAATTC AGGATGTGAC TCAGGAGAGC AAGGCTGTCT 
     2581 ACAGTACCAA CTGCCCAGTG TGGGAGGAAG CGTTCCGGTT CTTCCTACAA GACCCTCAAA 
     2641 GCCAGGAGCT CGATGTGCAA GTGAAGGATG ATTCCAGGGC CCTGACTTTA GGAGCACTGA 
     2701 CGCTGCCTCT GGCCCGCCTG CTGACTGCCC CAGAACTCAT CCTGGACCAG TGGTTCCAGC 
     2761 TCAGCAGCTC TGGTCCAAAC TCCAGACTCT ATATGAAACT AGTCATGAGG ATCCTGTACT 
     2821 TGGATTCATC AGAAATATGC TTCCCCACGG TGCCTGGTTG TCCTGGTGCT TGGGACGTGG 
     2881 ACAGTGAGAA TCCCCAGAGA GGCAGCAGTG TGGATGCCCC ACCTCGACCC TGTCACACGA 
     2941 CTCCTGATAG CCAGTTTGGG ACTGAGCATG TGCTTCGGAT CCATGTATTA GAGGCCCAGG 
     3001 ACCTGATTGC CAAAGACCGT TTCTTGGGGG GACTGGTGAA GGGCAAGTCA GACCCCTATG 
     3061 TCAAACTAAA GTTGGCAGGA CGAAGCTTCC GGAGCCATGT TGTTCGGGAA GATCTCAATC 
     3121 CCCGCTGGAA TGAGGTTTTT GAGGTGATCG TCACATCAGT TCCAGGCCAA GAGCTAGAGG 
     3181 TTGAAGTCTT TGACAAGGAC TTGGACAAGG ATGATTTTCT GGGCAGGTGT AAAGTGCGTC 
     3241 TCACCACAGT CTTAAACAGT GGCTTCCTTG ATGAGTGGCT GACCCTGGAG GATGTCCCAT 
     3301 CTGGCCGCCT GCACTTGCGC CTGGAGCGTC TCACCCCCCG TCCCACTGCT GCTGAGTTAG 
     3361 AGGAGGTGCT GCAGGTGAAT AGTTTGATCC AGACTCAGAA GAGTGCGGAG CTGGCTGCGG 
     3421 CCCTGCTATC CATCTATATG GAGCGGGCAG AGGACCTCCC GCTGCGAAAA GGCACCAAGC 
     3481 ACCTCAGCCC TTATGCTACT CTCACTGTGG GAGATAGTTC TCATAAAACC AAGACTATTT 
     3541 CGCAAACTTC AGCCCCTGTC TGGGATGAGA GTGCCTCCTT TCTCATCAGG AAACCACACA 
     3601 CTGAGAGCCT AGAGTTGCAG GTTCGGGGTG AGGGCACTGG CGTGCTGGGC TCATTATCCC 
     3661 TGCCCCTCTC AGAGCTCCTC GTGGCTGACC AGCTCTGCTT GGACCGCTGG TTTACACTCA 
     3721 GCAGTGGTCA GGGGCAGGTG CTACTGAGAG CACAGCTAGG GATCCTGGTG TCCCAGCACT 
     3781 CGGGAGTGGA AGCTCATAGC CACAGCTACA GCCACAGCTC CTCATCGCTG AGTGAAGAAC 
     3841 CAGAGCTCTC GGGGGGACCC CCTCACATCA CCTCCTCAGC CCCAGAGCTC CGGCAGCGCC 
     3901 TAACACATGT TGACAGTCCC CTTGAGGCTC CAGCCGGGCC TCTGGGCCAG GTGAAACTGA 
     3961 CTCTGTGGTA CTACAGTGAA GAACGAAAGC TGGTCAGCAT TGTTCATGGT TGCCGGTCCC 
     4021 TTCGACAGAA TGGACGTGAT CCTCCTGATC CCTATGTGTC ACTGTTGCTA CTGCCAGACA 
     4081 AGAACCGAGG CACCAAGAGG AGGACCTCAC AGAAGAAGAG GACCCTGAGT CCTGAATTTA 
     4141 ATGAACGGTT TGAGTGGGAA CTCCCCCTGG ATGAGGCCCA GAGACGAAAG CTGGATGTCT 
     4201 CTGTCAAGTC TAATTCCTCC TTCATGTCAA GAGAGCGTGA GCTGCTGGGG AAGGTGCAGC 
     4261 TGGACCTAGC TGAGACAGAC CTTTCCCAGG GTGTAGCCCG GTGGTATGAC CTGATGGACA 
     4321 ACAAGGACAA GGGCAGCTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4381 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5161 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5221 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5281 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5341 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5461 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5521 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5581 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5641 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5761 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5821 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5881 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5941 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6001 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6061 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6121 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6181 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6241 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6301 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6361 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6421 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6481 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6541 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6601 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6661 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6721 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6781 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6841 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6901 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6961 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7021 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7081 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7141 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7201 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7261 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7321 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7381 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7441 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7621 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7741 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7801 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7861 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7921 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7981 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8041 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8101 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8161 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//