LOCUS       RC201304L4                9000 bp    DNA     circular  SYN 
DEFINITION  GLA (Myc-DDK-tagged)-Human galactosidase, alpha (GLA)
ACCESSION   NM_000169
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7291..7471 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8199..8845 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6166..6186 
                     /label="LR50"  
     misc_feature    4629..5357 
                     /label="mGFP"  
     misc_feature    5364..5429 
                     /label="P2A"  
     misc_feature    7519..8138 
                     /label="pBR322 Ori"  
     misc_feature    5430..6029 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4608..4614 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4608 
                     /label="GLA"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCAGCTGAGG AACCCAGAAC TACATCTGGG 
     3361 CTGCGCGCTT GCGCTTCGCT TCCTGGCCCT CGTTTCCTGG GACATCCCTG GGGCTAGAGC 
     3421 ACTGGACAAT GGATTGGCAA GGACGCCTAC CATGGGCTGG CTGCACTGGG AGCGCTTCAT 
     3481 GTGCAACCTT GACTGCCAGG AAGAGCCAGA TTCCTGCATC AGTGAGAAGC TCTTCATGGA 
     3541 GATGGCAGAG CTCATGGTCT CAGAAGGCTG GAAGGATGCA GGTTATGAGT ACCTCTGCAT 
     3601 TGATGACTGT TGGATGGCTC CCCAAAGAGA TTCAGAAGGC AGACTTCAGG CAGACCCTCA 
     3661 GCGCTTTCCT CATGGGATTC GCCAGCTAGC TAATTATGTT CACAGCAAAG GACTGAAGCT 
     3721 AGGGATTTAT GCAGATGTTG GAAATAAAAC CTGCGCAGGC TTCCCTGGGA GTTTTGGATA 
     3781 CTACGACATT GATGCCCAGA CCTTTGCTGA CTGGGGAGTA GATCTGCTAA AATTTGATGG 
     3841 TTGTTACTGT GACAGTTTGG AAAATTTGGC AGATGGTTAT AAGCACATGT CCTTGGCCCT 
     3901 GAATAGGACT GGCAGAAGCA TTGTGTACTC CTGTGAGTGG CCTCTTTATA TGTGGCCCTT 
     3961 TCAAAAGCCC AATTATACAG AAATCCGACA GTACTGCAAT CACTGGCGAA ATTTTGCTGA 
     4021 CATTGATGAT TCCTGGAAAA GTATAAAGAG TATCTTGGAC TGGACATCTT TTAACCAGGA 
     4081 GAGAATTGTT GATGTTGCTG GACCAGGGGG TTGGAATGAC CCAGATATGT TAGTGATTGG 
     4141 CAACTTTGGC CTCAGCTGGA ATCAGCAAGT AACTCAGATG GCCCTCTGGG CTATCATGGC 
     4201 TGCTCCTTTA TTCATGTCTA ATGACCTCCG ACACATCAGC CCTCAAGCCA AAGCTCTCCT 
     4261 TCAGGATAAG GACGTAATTG CCATCAATCA GGACCCCTTG GGCAAGCAAG GGTACCAGCT 
     4321 TAGACAGGGA GACAACTTTG AAGTGTGGGA ACGACCTCTC TCAGGCTTAG CCTGGGCTGT 
     4381 AGCTATGATA AACCGGCAGG AGATTGGTGG ACCTCGCTCT TATACCATCG CAGTTGCTTC 
     4441 CCTGGGTAAA GGAGTGGCCT GTAATCCTGC CTGCTTCATC ACACAGCTCC TCCCTGTGAA 
     4501 AAGGAAGCTA GGGTTCTATG AATGGACTTC AAGGTTAAGA AGTCACATAA ATCCCACAGG 
     4561 CACTGTTTTG CTTCAGCTAG AAAATACAAT GCAGATGTCA TTAAAAGACT TACTTTACGC 
     4621 GTACGCGGCC GCTCGAGATG AGCGGGGGCG AGGAGCTGTT CGCCGGCATC GTGCCCGTGC 
     4681 TGATCGAGCT GGACGGCGAC GTGCACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGCGAGG 
     4741 GCGACGCCGA CTACGGCAAG CTGGAGATCA AGTTCATCTG CACCACCGGC AAGCTGCCCG 
     4801 TGCCCTGGCC CACCCTGGTG ACCACCCTCT GCTACGGCAT CCAGTGCTTC GCCCGCTACC 
     4861 CCGAGCACAT GAAGATGAAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACATCCAGG 
     4921 AGCGCACCAT CCAGTTCCAG GACGACGGCA AGTACAAGAC CCGCGGCGAG GTGAAGTTCG 
     4981 AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAA GGACTTCAAG GAGGACGGCA 
     5041 ACATCCTGGG CCACAAGCTG GAGTACAGCT TCAACAGCCA CAACGTGTAC ATCCGCCCCG 
     5101 ACAAGGCCAA CAACGGCCTG GAGGCTAACT TCAAGACCCG CCACAACATC GAGGGCGGCG 
     5161 GCGTGCAGCT GGCCGACCAC TACCAGACCA ACGTGCCCCT GGGCGACGGC CCCGTGCTGA 
     5221 TCCCCATCAA CCACTACCTG AGCACTCAGA CCAAGATCAG CAAGGACCGC AACGAGGCCC 
     5281 GCGACCACAT GGTGCTCCTG GAGTCCTTCA GCGCCTGCTG CCACACCCAC GGCATGGACG 
     5341 AGCTGTACAG GTCCGGACTC AGAGTTTGGG TAGGAAGCGG AGCTACTAAC TTCAGCCTGC 
     5401 TGAAGCAGGC TGGAGACGTG GAGGAGAACC CTGGACCTAT GACCGAGTAC AAGCCCACGG 
     5461 TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG 
     5521 CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGATCG CCACATCGAG CGGGTCACCG 
     5581 AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG 
     5641 ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT 
     5701 TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC 
     5761 AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG 
     5821 TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG 
     5881 TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC 
     5941 TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC 
     6001 CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGATG TACACCCAAA CGGCCGGCCG 
     6061 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     6121 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     6181 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     6241 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     6301 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     6361 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     6421 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     6481 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     6541 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     6601 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     6661 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     6721 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     6781 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     6841 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     6901 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     6961 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     7021 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     7081 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     7141 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     7201 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     7261 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     7321 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     7381 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     7441 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     7501 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     7561 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     7621 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     7681 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     7741 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     7801 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     7861 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     7921 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     7981 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     8041 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     8101 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     8161 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     8221 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     8281 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     8341 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     8401 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     8461 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     8521 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     8581 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     8641 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     8701 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     8761 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     8821 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     8881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8941 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//