LOCUS       RC201238                8202 bp    DNA     circular  SYN 
DEFINITION  POLD1 (Myc-DDK-tagged)-Human polymerase (DNA directed), delta 1, catalytic subunit 125kDa (POLD1)
ACCESSION   NM_002691
VERSION     NM_002691.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4359..4450 
                     /label="Myc-DDK"  
     misc_feature    4480..5065 
                     /label="PolyA signal"  
     misc_feature    4537..4556 
                     /label="Restriction Enzyme"  
     misc_feature    5341..6013 
                     /label="ColE1"  
     misc_feature    6443..7237 
                     /label="Kan/Neo"  
     misc_feature    7272..7574 
                     /label="SV40 ori"  
     misc_feature    7660..8137 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4341..4347 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4341 
                     /label="POLD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGGCAAG CGGCGGCCAG GCCCAGGGCC CGGGGTGCCC CCAAAGCGGG 
     1081 CCCGTGGGGG CCTCTGGGAT GATGATGATG CACCTCGGCC ATCCCAATTC GAGGAGGACC 
     1141 TGGCACTGAT GGAGGAGATG GAGGCAGAAC ACAGGCTGCA GGAGCAGGAG GAGGAGGAGC 
     1201 TGCAGTCAGT CCTGGAGGGG GTTGCAGACG GGCAGGTCCC ACCATCAGCC ATAGATCCTC 
     1261 GCTGGCTTCG GCCCACACCA CCAGCGCTGG ACCCCCAGAC AGAGCCCCTC ATCTTCCAAC 
     1321 AGTTGGAGAT TGACCATTAT GTGGGCCCAG CACAGCCTGT GCCTGGGGGG CCCCCACCAT 
     1381 CCCACGGCTC CGTGCCTGTG CTCCGCGCCT TCGGGGTCAC CGATGAGGGG TTCTCTGTCT 
     1441 GCTGCCACAT CCACGGCTTC GCTCCCTACT TCTACACCCC AGCGCCCCCT GGTTTCGGGC 
     1501 CCGAGCACAT GGGTGACCTG CAACGGGAGC TGAACTTGGC CATCAGCCGG GACAGTCGCG 
     1561 GGGGGAGGGA GCTGACTGGG CCGGCCGTGC TGGCTGTGGA ACTGTGCTCC CGAGAGAGCA 
     1621 TGTTTGGGTA CCACGGGCAC GGCCCCTCCC CGTTCCTGCG CATCACCGTG GCGCTGCCGC 
     1681 GCCTCGTGGC CCCGGCCCGC CGTCTCCTGG AACAGGGCAT CCGTGTGGCA GGCCTGGGCA 
     1741 CGCCCAGCTT CGCGCCCTAC GAGGCCAACG TCGACTTTGA GATCCGGTTC ATGGTGGACA 
     1801 CGGACATCGT CGGCTGCAAC TGGCTGGAGC TCCCAGCTGG GAAATACGCC CTGAGGCTGA 
     1861 AGGAGAAGGC TACGCAGTGC CAGCTGGAGG CGGACGTGCT GTGGTCTGAC GTGGTCAGTC 
     1921 ACCCACCGGA AGGGCCATGG CAGCGCATTG CGCCCTTGCG CGTGCTCAGC TTCGATATCG 
     1981 AGTGCGCCGG CCGCAAAGGC ATCTTCCCTG AGCCTGAGCG GGACCCTGTC ATCCAGATCT 
     2041 GCTCGCTGGG CCTGCGCTGG GGGGAGCCGG AGCCCTTCCT ACGCCTGGCG CTCACCCTGC 
     2101 GGCCCTGTGC CCCCATCCTG GGTGCCAAGG TGCAGAGCTA CGAGAAGGAG GAGGACCTGC 
     2161 TGCAGGCCTG GTCCACCTTC ATCCGTATCA TGGACCCCGA CGTGATCACC GGTTACAACA 
     2221 TCCAGAACTT CGACCTTCCG TACCTCATCT CTCGGGCCCA GACCCTCAAG GTACAAACAT 
     2281 TCCCTTTCCT GGGCCGTGTG GCCGGCCTTT GCTCCAACAT CCGGGACTCT TCATTCCAGT 
     2341 CCAAGCAGAC GGGCCGGCGG GACACCAAGG TTGTCAGCAT GGTGGGCCGC GTGCAGATGG 
     2401 ACATGCTGCA GGTGCTGCTG CGGGAGTACA AGCTCCGCTC CTACACGCTC AATGCCGTGA 
     2461 GCTTCCACTT CCTGGGCGAG CAGAAGGAGG ACGTGCAGCA CAGCATCATC ACCGACCTGC 
     2521 AGAATGGGAA CGACCAGACC CGCCGCCGCC TGGCTGTGTA CTGCCTGAAG GATGCCTACC 
     2581 TGCCACTGCG GCTGCTGGAG CGGCTCATGG TGCTGGTGAA CGCCGTGGAG ATGGCGAGGG 
     2641 TCACTGGCGT GCCCCTCAGC TACCTGCTCA GTCGTGGCCA GCAGGTCAAG GTCGTATCCC 
     2701 AGCTGTTGCG GCAGGCCATG CACGAGGGGC TGCTGATGCC CGTGGTGAAG TCAGAGGGCG 
     2761 GCGAGGACTA CACGGGAGCC ACTGTCATCG AGCCCCTCAA AGGGTACTAC GACGTCCCCA 
     2821 TCGCCACCCT GGACTTCTCC TCGCTGTACC CGTCCATCAT GATGGCCCAC AACCTGTGTT 
     2881 ACACCACGCT CCTTCGGCCC GGGACTGCAC AGAAACTGGG CCTGACTGAG GATCAGTTCA 
     2941 TCAGGACCCC CACCGGGGAC GAGTTTGTGA AGACCTCAGT GCGGAAGGGG CTGCTGCCCC 
     3001 AGATCCTGGA GAACCTGCTC AGTGCCCGGA AGAGGGCCAA GGCCGAGCTG GCCAAGGAGA 
     3061 CAGACCCCCT CCGGCGCCAG GTCCTGGATG GACGGCAGCT GGCGCTGAAG GTGAGCGCCA 
     3121 ACTCCGTATA CGGCTTCACT GGCGCCCAGG TGGGCAAGTT GCCGTGCCTG GAGATCTCAC 
     3181 AGAGCGTCAC GGGGTTCGGA CGTCAGATGA TCGAGAAAAC CAAGCAGCTG GTGGAGTCTA 
     3241 AGTACACAGT GGAGAATGGC TACAGCACCA GTGCCAAGGT GGTGTATGGT GACACTGACT 
     3301 CCGTCATGTG CCGATTCGGC GTGTCCTCGG TGGCTGAGGC GATGGCCCTG GGGCGGGAGG 
     3361 CCGCGGACTG GGTGTCAGGT CACTTCCCGT CGCCCATCCG GCTGGAGTTT GAGAAGGTCT 
     3421 ACTTCCCATA CCTGCTTATC AGCAAGAAGC GCTACGCGGG CCTGCTCTTC TCCTCCCGGC 
     3481 CCGACGCCCA CGACCGCATG GACTGCAAGG GCCTGGAGGC CGTGCGCAGG GACAACTGCC 
     3541 CCCTCGTGGC CAACCTGGTC ACTGCCTCAC TGCGCCGCCT GCTCATCGAC CGAGACCCTG 
     3601 AGGGCGCGGT GGCTCACGCA CAGGACGTCA TCTCGGACCT GCTGTGCAAC CGCATCGATA 
     3661 TCTCCCAGCT GGTCATCACC AAGGAGCTGA CCCGCGCGGC CTCCGACTAT GCCGGCAAGC 
     3721 AGGCCCACGT GGAGCTGGCC GAGAGGATGA GGAAGCGGGA CCCCGGGAGT GCGCCCAGCC 
     3781 TGGGCGACCG CGTCCCCTAC GTGATCATCA GTGCCGCCAA GGGTGTGGCC GCCTACATGA 
     3841 AGTCGGAGGA CCCGCTGTTC GTGCTGGAGC ACAGCCTGCC CATTGACACG CAGTACTACC 
     3901 TGGAGCAGCA GCTGGCCAAG CCCCTCCTGC GCATCTTCGA GCCCATCCTG GGCGAGGGCC 
     3961 GTGCCGAGGC TGTGCTACTG CGGGGGGACC ACACGCGCTG CAAGACGGTG CTCACGGGCA 
     4021 AGGTGGGCGG CCTCCTGGCC TTCGCCAAAC GCCGCAACTG CTGCATTGGC TGCCGCACAG 
     4081 TGCTCAGCCA CCAGGGAGCC GTGTGTGAGT TCTGCCAGCC CCGGGAGTCT GAGCTGTATC 
     4141 AGAAGGAGGT ATCCCATCTG AATGCCCTGG AGGAGCGCTT CTCGCGCCTC TGGACGCAGT 
     4201 GCCAGCGCTG CCAGGGCAGC CTGCACGAGG ACGTCATCTG CACCAGCCGG GACTGCCCCA 
     4261 TCTTCTACAT GCGCAAGAAG GTGCGGAAGG ACCTGGAAGA CCAGGAGCAG CTCCTGCGGC 
     4321 GCTTCGGACC CCCTGGACCT GAGGCCTGGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4381 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4441 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4501 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4561 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4621 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4681 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4741 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4801 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4861 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4921 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4981 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5041 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5101 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5161 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5221 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5281 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5341 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5401 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5461 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5521 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5581 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5641 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5701 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5761 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5821 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5881 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5941 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6001 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6061 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6121 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6181 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6241 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6301 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6361 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6421 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6481 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6541 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6601 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6661 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6721 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6781 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6841 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6901 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6961 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7021 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7081 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7141 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7201 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7261 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7321 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7381 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7441 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7501 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7621 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7681 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7741 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7801 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7861 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7921 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7981 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8041 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8101 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8161 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//