LOCUS       RC201151                8028 bp    DNA     circular  SYN 
DEFINITION  ITGA5 (Myc-DDK-tagged)-Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5)
ACCESSION   NM_002205
VERSION     NM_002205.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4185..4276 
                     /label="Myc-DDK"  
     misc_feature    4306..4891 
                     /label="PolyA signal"  
     misc_feature    4363..4382 
                     /label="Restriction Enzyme"  
     misc_feature    5167..5839 
                     /label="ColE1"  
     misc_feature    6269..7063 
                     /label="Kan/Neo"  
     misc_feature    7098..7400 
                     /label="SV40 ori"  
     misc_feature    7486..7963 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4167..4173 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4167 
                     /label="ITGA5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAGCCGG ACGCCAGAGT CCCCTCTCCA CGCCGTGCAG CTGCGCTGGG 
     1081 GCCCCCGGCG CCGACCCCCG CTGCTGCCGC TGCTGTTGCT GCTGCTGCCG CCGCCACCCA 
     1141 GGGTCGGGGG CTTCAACTTA GACGCGGAGG CCCCAGCAGT ACTCTCGGGG CCCCCGGGCT 
     1201 CCTTCTTCGG ATTCTCAGTG GAGTTTTACC GGCCGGGAAC AGACGGGGTC AGTGTGCTGG 
     1261 TGGGAGCACC CAAGGCTAAT ACCAGCCAGC CAGGAGTGCT GCAGGGTGGT GCTGTCTACC 
     1321 TCTGTCCTTG GGGTGCCAGC CCCACACAGT GCACCCCCAT TGAATTTGAC AGCAAAGGCT 
     1381 CTCGGCTCCT GGAGTCCTCA CTGTCCAGCT CAGAGGGAGA GGAGCCTGTG GAGTACAAGT 
     1441 CCTTGCAGTG GTTCGGGGCA ACAGTTCGAG CCCATGGCTC CTCCATCTTG GCATGCGCTC 
     1501 CACTGTACAG CTGGCGCACA GAGAAGGAGC CACTGAGCGA CCCCGTGGGC ACCTGCTACC 
     1561 TCTCCACAGA TAACTTCACC CGAATTCTGG AGTATGCACC CTGCCGCTCA GATTTCAGCT 
     1621 GGGCAGCAGG ACAGGGTTAC TGCCAAGGAG GCTTCAGTGC CGAGTTCACC AAGACTGGCC 
     1681 GTGTGGTTTT AGGTGGACCA GGAAGCTATT TCTGGCAAGG CCAGATCCTG TCTGCCACTC 
     1741 AGGAGCAGAT TGCAGAATCT TATTACCCCG AGTACCTGAT CAACCTGGTT CAGGGGCAGC 
     1801 TGCAGACTCG CCAGGCCAGT TCCATCTATG ATGACAGCTA CCTAGGATAC TCTGTGGCTG 
     1861 TTGGTGAATT CAGTGGTGAT GACACAGAAG ACTTTGTTGC TGGTGTGCCC AAAGGGAACC 
     1921 TCACTTACGG CTATGTCACC ATCCTTAATG GCTCAGACAT TCGATCCCTC TACAACTTCT 
     1981 CAGGGGAACA GATGGCCTCC TACTTTGGCT ATGCAGTGGC CGCCACAGAC GTCAATGGGG 
     2041 ACGGGCTGGA TGACTTGCTG GTGGGGGCAC CCCTGCTCAT GGATCGGACC CCTGACGGGC 
     2101 GGCCTCAGGA GGTGGGCAGG GTCTACGTCT ACCTGCAGCA CCCAGCCGGC ATAGAGCCCA 
     2161 CGCCCACCCT TACCCTCACT GGCCATGATG AGTTTGGCCG ATTTGGCAGC TCCTTGACCC 
     2221 CCCTGGGGGA CCTGGACCAG GATGGCTACA ATGATGTGGC CATCGGGGCT CCCTTTGGTG 
     2281 GGGAGACCCA GCAGGGAGTA GTGTTTGTAT TTCCTGGGGG CCCAGGAGGG CTGGGCTCTA 
     2341 AGCCTTCCCA GGTTCTGCAG CCCCTGTGGG CAGCCAGCCA CACCCCAGAC TTCTTTGGCT 
     2401 CTGCCCTTCG AGGAGGCCGA GACCTGGATG GCAATGGATA TCCTGATCTG ATTGTGGGGT 
     2461 CCTTTGGTGT GGACAAGGCT GTGGTATACA GGGGCCGCCC CATCGTGTCC GCTAGTGCCT 
     2521 CCCTCACCAT CTTCCCCGCC ATGTTCAACC CAGAGGAGCG GAGCTGCAGC TTAGAGGGGA 
     2581 ACCCTGTGGC CTGCATCAAC CTTAGCTTCT GCCTCAATGC TTCTGGAAAA CACGTTGCTG 
     2641 ACTCCATTGG TTTCACAGTG GAACTTCAGC TGGACTGGCA GAAGCAGAAG GGAGGGGTAC 
     2701 GGCGGGCACT GTTCCTGGCC TCCAGGCAGG CAACCCTGAC CCAGACCCTG CTCATCCAGA 
     2761 ATGGGGCTCG AGAGGATTGC AGAGAGATGA AGATCTACCT CAGGAACGAG TCAGAATTTC 
     2821 GAGACAAACT CTCGCCGATT CACATCGCTC TCAACTTCTC CTTGGACCCC CAAGCCCCAG 
     2881 TGGACAGCCA CGGCCTCAGG CCAGCCCTAC ATTATCAGAG CAAGAGCCGG ATAGAGGACA 
     2941 AGGCTCAGAT CTTGCTGGAC TGTGGAGAAG ACAACATCTG TGTGCCTGAC CTGCAGCTGG 
     3001 AAGTGTTTGG GGAGCAGAAC CATGTGTACC TGGGTGACAA GAATGCCCTG AACCTCACTT 
     3061 TCCATGCCCA GAATGTGGGT GAGGGTGGCG CCTATGAGGC TGAGCTTCGG GTCACCGCCC 
     3121 CTCCAGAGGC TGAGTACTCA GGACTCGTCA GACACCCAGG GAACTTCTCC AGCCTGAGCT 
     3181 GTGACTACTT TGCCGTGAAC CAGAGCCGCC TGCTGGTGTG TGACCTGGGC AACCCCATGA 
     3241 AGGCAGGAGC CAGTCTGTGG GGTGGCCTTC GGTTTACAGT CCCTCATCTC CGGGACACTA 
     3301 AGAAAACCAT CCAGTTTGAC TTCCAGATCC TCAGCAAGAA TCTCAACAAC TCGCAAAGCG 
     3361 ACGTGGTTTC CTTTCGGCTC TCCGTGGAGG CTCAGGCCCA GGTCACCCTG AACGGTGTCT 
     3421 CCAAGCCTGA GGCAGTGCTA TTCCCAGTAA GCGACTGGCA TCCCCGAGAC CAGCCTCAGA 
     3481 AGGAGGAGGA CCTGGGACCT GCTGTCCACC ATGTCTATGA GCTCATCAAC CAAGGCCCCA 
     3541 GCTCCATTAG CCAGGGTGTG CTGGAACTCA GCTGTCCCCA GGCTCTGGAA GGTCAGCAGC 
     3601 TCCTATATGT GACCAGAGTT ACGGGACTCA ACTGCACCAC CAATCACCCC ATTAACCCAA 
     3661 AGGGCCTGGA GTTGGATCCC GAGGGTTCCC TGCACCACCA GCAAAAACGG GAAGCTCCAA 
     3721 GCCGCAGCTC TGCTTCCTCG GGACCTCAGA TCCTGAAATG CCCGGAGGCT GAGTGTTTCA 
     3781 GGCTGCGCTG TGAGCTCGGG CCCCTGCACC AACAAGAGAG CCAAAGTCTG CAGTTGCATT 
     3841 TCCGAGTCTG GGCCAAGACT TTCTTGCAGC GGGAGCACCA GCCATTTAGC CTGCAGTGTG 
     3901 AGGCTGTGTA CAAAGCCCTG AAGATGCCCT ACCGAATCCT GCCTCGGCAG CTGCCCCAAA 
     3961 AAGAGCGTCA GGTGGCCACA GCTGTGCAAT GGACCAAGGC AGAAGGCAGC TATGGCGTCC 
     4021 CACTGTGGAT CATCATCCTA GCCATCCTGT TTGGCCTCCT GCTCCTAGGT CTACTCATCT 
     4081 ACATCCTCTA CAAGCTTGGA TTCTTCAAAC GCTCCCTCCC ATATGGCACC GCCATGGAAA 
     4141 AAGCTCAGCT CAAGCCTCCA GCCACCTCTG ATGCCACGCG TACGCGGCCG CTCGAGCAGA 
     4201 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4261 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4321 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4381 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4441 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4501 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4561 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4621 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4681 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4741 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4801 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4861 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4921 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4981 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5041 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5101 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5161 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5221 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5281 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5341 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5401 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5461 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5521 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5581 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5641 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5701 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5761 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5821 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5881 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5941 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6001 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6061 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6121 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6181 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6241 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6301 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6361 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6421 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6481 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6541 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6601 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6661 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6721 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6781 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6841 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6901 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6961 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7021 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7081 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7141 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7201 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7261 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7441 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7561 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7621 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7681 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7741 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7801 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7861 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7921 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7981 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//