LOCUS       RC201121                7575 bp    DNA     circular  SYN 
DEFINITION  DAG1 (Myc-DDK-tagged)-Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1), transcript variant 2
ACCESSION   NM_004393
VERSION     NM_004393.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3730..3821 
                     /label="Myc-DDK"  
     misc_feature    3851..4436 
                     /label="PolyA signal"  
     misc_feature    3908..3927 
                     /label="Restriction Enzyme"  
     misc_feature    4712..5384 
                     /label="ColE1"  
     misc_feature    5814..6608 
                     /label="Kan/Neo"  
     misc_feature    6643..6945 
                     /label="SV40 ori"  
     misc_feature    7031..7508 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3705..3712 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3705 
                     /label="DAG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGATGTCT GTGGGCCTCT CGCTGCTGCT GCCCCTCTGG GGGAGGACCT 
     1081 TTCTCCTCCT GCTCTCTGTG GTTATGGCTC AGTCCCACTG GCCCAGTGAA CCCTCAGAGG 
     1141 CTGTCAGGGA CTGGGAAAAC CAGCTTGAGG CATCCATGCA CTCAGTGCTC TCAGACCTCC 
     1201 ACGAGGCTGT TCCCACAGTG GTTGGCATTC CTGATGGCAC GGCTGTCGTC GGGCGCTCAT 
     1261 TTCGAGTGAC CATTCCAACA GATTTGATTG CCTCCAGTGG AGATATCATC AAGGTATCAG 
     1321 CGGCAGGGAA GGAGGCTTTG CCATCTTGGC TGCACTGGGA CTCACAGAGC CACACCCTGG 
     1381 AGGGCCTCCC CCTTGACACT GATAAGGGTG TGCATTACAT TTCAGTGAGC GCTACACGGC 
     1441 TGGGGGCCAA CGGGAGCCAC ATCCCCCAGA CCTCCAGTGT GTTCTCCATC GAGGTCTACC 
     1501 CTGAAGACCA CAGTGAGCTG CAGTCGGTGA GGACAGCCTC CCCAGACCCT GGTGAGGTGG 
     1561 TATCATCTGC CTGTGCTGCG GATGAACCTG TGACTGTTTT GACGGTGATT TTGGATGCCG 
     1621 ACCTCACCAA GATGACCCCA AAGCAAAGGA TTGACCTCCT GCACAGGATG CGGAGCTTCT 
     1681 CAGAAGTAGA GCTTCACAAC ATGAAATTAG TGCCGGTGGT GAATAACAGA CTATTTGACA 
     1741 TGTCGGCCTT CATGGCTGGC CCGGGAAATG CAAAAAAGGT GGTGGAGAAT GGGGCCCTTC 
     1801 TCTCCTGGAA GCTGGGCTGC TCCCTGAACC AGAACAGTGT GCCTGACATT CATGGTGTAG 
     1861 AGGCCCCTGC CAGGGAGGGC GCAATGTCTG CTCAGCTTGG CTACCCTGTG GTGGGTTGGC 
     1921 ACATCGCCAA TAAGAAGCCC CCTCTTCCCA AACGCGTCCG GAGGCAGATC CATGCTACAC 
     1981 CCACACCTGT CACTGCCATT GGGCCCCCAA CCACGGCTAT CCAGGAGCCC CCATCCAGGA 
     2041 TCGTGCCAAC CCCCACATCT CCAGCCATTG CTCCTCCAAC AGAGACCATG GCTCCTCCAG 
     2101 TCAGGGATCC TGTTCCTGGG AAACCCACGG TCACCATCCG GACTCGAGGC GCCATTATTC 
     2161 AAACCCCAAC CCTAGGCCCC ATCCAGCCTA CTCGGGTGTC AGAAGCTGGC ACCACAGTTC 
     2221 CTGGCCAGAT TCGCCCAACG ATGACCATTC CTGGCTATGT GGAGCCTACT GCAGTTGCTA 
     2281 CCCCTCCCAC AACCACCACC AAGAAGCCAC GAGTATCCAC ACCAAAACCA GCAACGCCTT 
     2341 CAACTGACTC CACCACCACC ACGACTCGCA GGCCAACCAA GAAACCACGG ACACCCCGGC 
     2401 CAGTGCCCCG GGTCACCACC AAAGTTTCCA TCACCAGATT GGAAACTGCC TCACCGCCTA 
     2461 CTCGTATTCG CACCACCACC AGTGGAGTGC CCCGTGGCGG AGAACCCAAC CAGCGCCCAG 
     2521 AGCTCAAGAA CCATATTGAC AGGGTAGATG CCTGGGTTGG CACCTACTTT GAGGTGAAGA 
     2581 TCCCGTCAGA CACTTTCTAT GACCATGAGG ACACCACCAC TGACAAGCTG AAGCTGACCC 
     2641 TGAAACTGCG GGAGCAGCAG CTGGTGGGCG AGAAGTCCTG GGTACAGTTC AACAGCAACA 
     2701 GCCAGCTCAT GTATGGCCTT CCCGACAGCA GCCACGTGGG CAAACACGAG TATTTCATGC 
     2761 ATGCCACAGA CAAGGGGGGC CTGTCGGCTG TGGATGCCTT CGAGATCCAC GTCCACAGGC 
     2821 GCCCCCAAGG GGATAGGGCT CCTGCAAGGT TCAAGGCCAA GTTTGTGGGT GACCCGGCAC 
     2881 TGGTGTTGAA TGACATCCAC AAGAAGATTG CCTTGGTAAA GAAACTGGCC TTCGCCTTTG 
     2941 GAGACCGAAA CTGTAGCACC ATCACCCTGC AGAATATCAC CCGGGGCTCC ATCGTGGTGG 
     3001 AATGGACCAA CAACACACTG CCCTTGGAGC CCTGCCCCAA GGAGCAGATC GCTGGGCTGA 
     3061 GCCGCCGGAT CGCTGAGGAT GATGGAAAAC CTCGGCCTGC CTTCTCCAAC GCCCTAGAGC 
     3121 CTGACTTTAA GGCCACAAGC ATCACTGTGA CGGGCTCTGG CAGTTGTCGG CACCTACAGT 
     3181 TTATCCCTGT GGTACCACCC AGGAGAGTGC CCTCAGAGGC GCCGCCCACA GAAGTGCCTG 
     3241 ACAGGGACCC TGAGAAGAGC AGTGAGGATG ATGTCTACCT GCATACAGTC ATTCCGGCCG 
     3301 TGGTGGTCGC AGCCATCCTG CTCATTGCTG GCATCATTGC CATGATCTGC TACCGCAAGA 
     3361 AGCGGAAGGG CAAGCTTACC CTTGAGGACC AGGCCACCTT CATCAAGAAG GGGGTGCCTA 
     3421 TCATCTTTGC AGACGAACTG GACGACTCCA AGCCCCCACC CTCCTCCAGC ATGCCACTCA 
     3481 TTCTGCAGGA GGAGAAGGCT CCCCTACCCC CTCCTGAGTA CCCCAACCAG AGTGTGCCCG 
     3541 AGACCACTCC TCTGAACCAG GACACCATGG GAGAGTACAC GCCCCTGCGG GATGAGGATC 
     3601 CCAATGCGCC TCCCTACCAG CCCCCACCGC CCTTCACAGC ACCCATGGAG GGCAAGGGCT 
     3661 CCCGTCCCAA GAACATGACC CCATACCGGT CACCTCCTCC CTATGTCCCA CCTAGCGGAC 
     3721 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3781 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3841 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3901 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3961 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4021 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4081 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4141 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4201 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4261 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4321 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4381 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4441 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4501 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4561 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4621 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4681 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4741 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4801 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4861 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4921 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4981 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5041 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5101 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5161 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5221 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5281 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5341 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5401 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5461 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5521 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5581 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5641 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5701 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5761 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5821 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5881 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5941 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6001 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6061 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6121 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6181 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6241 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6301 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6361 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6421 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6481 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6541 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6601 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6661 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6721 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6781 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6841 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6901 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6961 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7021 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7081 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7141 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7201 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7261 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7321 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7381 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7441 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7501 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7561 TTTAACGCGA ATTTT
//