LOCUS       RC201114                7323 bp    DNA     circular  SYN 
DEFINITION  CDH15 (Myc-DDK-tagged)-Human cadherin 15, type 1, M-cadherin (myotubule) (CDH15)
ACCESSION   NM_004933
VERSION     NM_004933.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3480..3571 
                     /label="Myc-DDK"  
     misc_feature    3601..4186 
                     /label="PolyA signal"  
     misc_feature    3658..3677 
                     /label="Restriction Enzyme"  
     misc_feature    4462..5134 
                     /label="ColE1"  
     misc_feature    5564..6358 
                     /label="Kan/Neo"  
     misc_feature    6393..6695 
                     /label="SV40 ori"  
     misc_feature    6781..7258 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3462..3468 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3462 
                     /label="CDH15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGCCGCG TTCCTCCTCG TCCTCGGGCT GTTGGCCCAG AGCCTCTGCC 
     1081 TGTCTTTGGG GGTTCCTGGA TGGAGGAGGC CCACCACCCT GTACCCCTGG CGCCGGGCGC 
     1141 CTGCCCTGAG CCGCGTGCGG AGGGCCTGGG TCATCCCCCC GATCAGCGTA TCCGAGAACC 
     1201 ACAAGCGTCT CCCCTACCCC CTGGTTCAGA TCAAGTCGGA CAAGCAGCAG CTGGGCAGCG 
     1261 TCATCTACAG CATCCAGGGA CCCGGCGTGG ATGAGGAGCC CCGGGGCGTC TTCTCTATCG 
     1321 ACAAGTTCAC AGGGAAGGTC TTCCTCAATG CCATGCTGGA CCGCGAGAAG ACTGATCGCT 
     1381 TCAGGCTAAG AGCGTTTGCC CTGGACCTGG GAGGATCCAC CCTGGAGGAC CCCACGGACC 
     1441 TGGAGATTGT AGTTGTGGAT CAGAATGACA ACCGGCCAGC CTTCCTGCAG GAGGCGTTCA 
     1501 CTGGCCGCGT GCTGGAGGGT GCAGTCCCAG GCACCTATGT GACCAGGGCA GAGGCCACAG 
     1561 ATGCCGACGA CCCCGAGACG GACAACGCAG CGCTGCGGTT CTCCATCCTG CAGCAGGGCA 
     1621 GCCCCGAGCT CTTCAGCATC GACGAGCTCA CAGGAGAGAT CCGCACAGTG CAAGTGGGGC 
     1681 TGGACCGCGA GGTGGTCGCG GTGTACAATC TGACCCTGCA GGTGGCGGAC ATGTCTGGAG 
     1741 ACGGCCTCAC AGCCACTGCC TCAGCCATCA TCACCCTTGA TGACATCAAT GACAATGCCC 
     1801 CCGAGTTCAC CAGGGATGAG TTCTTCATGG AGGCCATAGA GGCCGTCAGC GGAGTGGATG 
     1861 TGGGACGCCT GGAAGTGGAG GACAGGGACC TGCCAGGCTC CCCAAACTGG GTGGCCAGGT 
     1921 TCACTATCCT GGAAGGCGAC CCCGATGGGC AGTTCACCAT CCGCACGGAC CCCAAGACCA 
     1981 ACGAGGGTGT TCTGTCCATT GTGAAGGCCC TGGACTATGA GAGCTGTGAA CACTACGAAC 
     2041 TCAAAGTGTC GGTGCAGAAT GAGGCCCCGC TGCAGGCGGC TGCCCTTAGG GCTGAGCGGG 
     2101 GCCAGGCCAA GGTCCGCGTG CATGTGCAGG ACACCAACGA GCCCCCCGTG TTCCAGGAGA 
     2161 ACCCACTTCG GACCAGCCTA GCAGAGGGGG CACCCCCAGG CACTCTGGTG GCCACCTTCT 
     2221 CTGCCCGGGA CCCTGACACA GAGCAGCTGC AGAGGCTCAG CTACTCCAAG GACTACGACC 
     2281 CGGAAGACTG GCTGCAAGTG GACGCAGCCA CTGGCCGGAT CCAGACCCAG CACGTGCTCA 
     2341 GCCCGGCGTC CCCCTTCCTC AAGGGCGGCT GGTACAGAGC CATCGTCCTG GCCCAGGATG 
     2401 ACGCCTCCCA GCCCCGCACC GCCACCGGCA CCCTGTCCAT CGAGATCCTG GAGGTGAACG 
     2461 ACCATGCACC TGTGCTGGCC CCGCCGCCGC CGGGCAGCCT GTGCAGCGAG CCACACCAAG 
     2521 GCCCAGGCCT CCTCCTGGGC GCCACGGATG AGGACCTGCC CCCCCACGGG GCCCCCTTCC 
     2581 ACTTCCAGCT GAGCCCCAGG CTCCCAGAGC TCGGCCGGAA CTGGAGCCTC AGCCAGGTCA 
     2641 ACGTGAGCCA CGCGCGCCTG CGGCCGCGAC ACCAGGTCCC CGAAGGCCTG CACCGCCTCA 
     2701 GCCTGCTGCT CCGGGACTCG GGGCAGCCGC CCCAGCAGCG CGAGCAGCCT CTGAACGTGA 
     2761 CCGTGTGCCG CTGCGGCAAG GACGGCGTCT GCCTGCCGGG GGCCGCAGCG CTGCTGGCGG 
     2821 GGGGCACAGG CCTCAGCCTG GGCGCACTGG TCATCGTGCT GGCCAGCGCC CTCCTGCTGC 
     2881 TGGTGCTGGT CCTGCTCGTG GCACTCCGGG CGCGGTTCTG GAAGCAGTCT CGGGGCAAGG 
     2941 GGCTGCTGCA CGGCCCCCAG GACGACCTTC GAGACAATGT CCTCAACTAC GATGAGCAAG 
     3001 GAGGCGGGGA GGAGGACCAG GACGCCTACG ACATCAGCCA GCTGCGTCAC CCGACAGCGC 
     3061 TGAGCCTGCC TCTGGGACCG CCGCCACTTC GCAGAGATGC CCCGCAGGGC CGCCTGCACC 
     3121 CCCAGCCACC CCGAGTGCTG CCCACCAGCC CCCTGGACAT CGCCGACTTC ATCAATGATG 
     3181 GCTTGGAGGC TGCAGATAGT GACCCCAGTG TGCCGCCTTA CGACACAGCC CTCATCTATG 
     3241 ACTACGAGGG TGACGGCTCG GTGGCGGGGA CGCTGAGCTC CATCCTGTCC AGCCAGGGCG 
     3301 ATGAGGACCA GGACTACGAC TACCTCAGAG ACTGGGGGCC CCGCTTCGCC CGGCTGGCAG 
     3361 ACATGTATGG GCACCCGTGC GGGTTGGAGT ACGGGGCCAG ATGGGACCAC CAGGCCAGGG 
     3421 AGGGTCTTTC TCCTGGGGCA CTGCTACCCA GACACAGAGG CCGGACAGCC ACGCGTACGC 
     3481 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3541 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3601 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3661 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3721 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3781 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3841 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3901 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3961 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4021 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4081 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4141 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4201 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4261 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4321 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4381 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4441 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4501 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4561 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4621 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4681 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4741 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4801 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4861 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4921 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4981 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5041 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5101 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5161 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5221 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5281 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5341 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5401 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5461 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5521 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5581 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5641 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5701 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5761 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5821 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5881 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5941 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6001 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6061 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6121 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6181 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6241 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6301 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6361 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6421 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6481 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6541 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6601 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6661 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6721 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6781 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6841 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6901 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6961 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7021 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7081 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7141 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7201 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7261 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7321 TTT
//