LOCUS       RC200659                7509 bp    DNA     circular  SYN 
DEFINITION  KPNB1 (Myc-DDK-tagged)-Human karyopherin (importin) beta 1 (KPNB1)
ACCESSION   NM_002265
VERSION     NM_002265.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3666..3757 
                     /label="Myc-DDK"  
     misc_feature    3787..4372 
                     /label="PolyA signal"  
     misc_feature    3844..3863 
                     /label="Restriction Enzyme"  
     misc_feature    4648..5320 
                     /label="ColE1"  
     misc_feature    5750..6544 
                     /label="Kan/Neo"  
     misc_feature    6579..6881 
                     /label="SV40 ori"  
     misc_feature    6967..7444 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3648..3654 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3648 
                     /label="KPNB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGATC ACCATTCTCG AGAAGACCGT GTCTCCCGAT CGGCTGGAGC 
     1081 TGGAAGCGGC GCAGAAGTTC CTGGAGCGTG CGGCCGTGGA GAACCTGCCC ACTTTCCTTG 
     1141 TGGAACTGTC CAGAGTGCTG GCAAATCCAG GAAACAGTCA GGTTGCCAGA GTTGCAGCTG 
     1201 GTCTACAAAT CAAGAACTCT TTGACATCTA AAGATCCAGA TATCAAGGCA CAATATCAGC 
     1261 AGAGGTGGCT TGCTATTGAT GCTAATGCTC GACGAGAAGT CAAGAACTAT GTTTTGCAGA 
     1321 CATTGGGTAC AGAAACTTAC CGGCCTAGTT CTGCCTCACA GTGTGTGGCT GGTATTGCTT 
     1381 GTGCAGAGAT CCCAGTAAAC CAGTGGCCAG AACTCATTCC TCAGCTGGTG GCCAATGTCA 
     1441 CAAACCCCAA CAGCACAGAG CACATGAAGG AGTCGACATT GGAAGCCATC GGTTATATTT 
     1501 GCCAAGATAT AGACCCAGAG CAGCTACAAG ATAAATCCAA TGAGATTCTG ACTGCCATAA 
     1561 TCCAGGGGAT GAGGAAAGAA GAGCCTAGTA ATAATGTGAA GCTAGCTGCT ACGAATGCAC 
     1621 TCCTGAACTC ATTGGAGTTC ACCAAAGCAA ACTTTGATAA AGAGTCTGAA AGGCACTTTA 
     1681 TTATGCAGGT GGTCTGTGAA GCCACACAGT GTCCAGATAC GAGGGTACGA GTGGCTGCTT 
     1741 TACAGAATCT GGTGAAGATA ATGTCCTTAT ATTATCAGTA CATGGAGACA TATATGGGTC 
     1801 CTGCTCTTTT TGCAATCACA ATCGAAGCAA TGAAAAGTGA CATTGATGAG GTGGCTTTAC 
     1861 AAGGGATAGA ATTCTGGTCC AATGTCTGTG ATGAGGAAAT GGATTTGGCC ATTGAAGCTT 
     1921 CAGAGGCAGC AGAACAAGGA CGGCCCCCTG AGCACACCAG CAAGTTTTAT GCGAAGGGAG 
     1981 CACTACAGTA TCTGGTTCCA ATCCTCACAC AGACACTAAC TAAACAGGAC GAAAATGATG 
     2041 ATGACGATGA CTGGAACCCC TGCAAAGCAG CAGGGGTGTG CCTCATGCTT CTGGCCACCT 
     2101 GCTGTGAAGA TGACATTGTC CCACATGTCC TCCCCTTCAT TAAAGAACAC ATCAAGAACC 
     2161 CAGATTGGCG GTACCGGGAT GCAGCAGTGA TGGCTTTTGG TTGTATCTTG GAAGGACCAG 
     2221 AGCCCAGTCA GCTCAAACCA CTAGTTATAC AGGCTATGCC CACCCTAATA GAATTAATGA 
     2281 AAGACCCCAG TGTAGTTGTT CGAGATACAG CTGCATGGAC TGTAGGCAGA ATTTGTGAGC 
     2341 TGCTTCCTGA AGCTGCCATC AATGATGTCT ACTTGGCTCC CCTGCTACAG TGTCTGATTG 
     2401 AGGGTCTCAG TGCTGAACCC AGAGTGGCTT CAAATGTGTG CTGGGCTTTC TCCAGTCTGG 
     2461 CTGAAGCTGC TTATGAAGCT GCAGACGTTG CTGATGATCA GGAAGAACCA GCTACTTACT 
     2521 GCTTATCTTC TTCATTTGAA CTCATAGTTC AGAAGCTCCT AGAGACTACA GACAGACCTG 
     2581 ATGGACACCA GAACAACCTG AGGAGTTCTG CATATGAATC TCTGATGGAA ATTGTGAAAA 
     2641 ACAGTGCCAA GGATTGTTAT CCTGCTGTCC AGAAAACGAC TTTGGTCATC ATGGAACGAC 
     2701 TGCAACAGGT TCTTCAGATG GAGTCACATA TCCAGAGCAC ATCCGATAGA ATCCAGTTCA 
     2761 ATGACCTTCA GTCTTTACTC TGTGCAACTC TTCAGAATGT TCTTCGGAAA GTGCAACATC 
     2821 AAGATGCTTT GCAGATCTCT GATGTGGTTA TGGCCTCCCT GTTAAGGATG TTCCAAAGCA 
     2881 CAGCTGGGTC TGGGGGAGTA CAAGAGGATG CCCTGATGGC AGTTAGCACA CTGGTGGAAG 
     2941 TGTTGGGTGG TGAATTCCTC AAGTACATGG AGGCCTTTAA ACCCTTCCTG GGCATTGGAT 
     3001 TAAAAAATTA TGCTGAATAC CAGGTTTGTT TGGCAGCTGT GGGCTTAGTG GGAGACTTGT 
     3061 GCCGTGCCCT GCAATCCAAC ATCATACCTT TCTGTGACGA GGTGATGCAG CTGCTTCTGG 
     3121 AAAATTTGGG GAATGAGAAC GTCCACAGGT CTGTGAAGCC GCAGATTCTG TCAGTGTTTG 
     3181 GTGATATTGC CCTTGCTATT GGAGGAGAGT TTAAAAAATA CTTAGAGGTT GTATTGAATA 
     3241 CTCTTCAGCA GGCCTCCCAA GCCCAGGTGG ACAAGTCAGA CTATGACATG GTGGATTATC 
     3301 TGAATGAGCT AAGGGAAAGC TGCTTGGAAG CCTATACTGG AATCGTCCAG GGATTAAAGG 
     3361 GGGATCAGGA GAACGTACAC CCGGATGTGA TGCTGGTACA ACCCAGAGTA GAATTTATTC 
     3421 TGTCTTTCAT TGACCACATT GCTGGAGATG AGGATCACAC AGATGGAGTA GTAGCTTGTG 
     3481 CTGCTGGACT AATAGGGGAC TTATGTACAG CATTTGGGAA GGATGTACTG AAATTAGTAG 
     3541 AAGCTAGGCC AATGATCCAT GAATTGTTAA CTGAAGGGCG GAGATCGAAG ACTAACAAAG 
     3601 CAAAAACCCT TGCTACATGG GCAACAAAAG AACTGAGGAA ACTGAAGAAC CAAGCTACGC 
     3661 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3721 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3781 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3841 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3901 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3961 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4021 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4081 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4141 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4201 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4261 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4321 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4381 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4441 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4501 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4561 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4621 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4681 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4741 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4801 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4861 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4921 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4981 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5041 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5101 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5161 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5221 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5281 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5341 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5401 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5461 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5521 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5581 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5641 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5701 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5761 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5821 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5881 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5941 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6001 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6061 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6121 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6181 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6241 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6301 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6361 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6421 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6481 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6541 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6601 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6661 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6721 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6781 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6841 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6901 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6961 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7021 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7081 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7141 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7201 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7261 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7321 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7381 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7441 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7501 GCGAATTTT
//