LOCUS       RC200638                7914 bp    DNA     circular  SYN 
DEFINITION  MAN2B1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1
ACCESSION   NM_000528
VERSION     NM_000528.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4071..4162 
                     /label="Myc-DDK"  
     misc_feature    4192..4777 
                     /label="PolyA signal"  
     misc_feature    4249..4268 
                     /label="Restriction Enzyme"  
     misc_feature    5053..5725 
                     /label="ColE1"  
     misc_feature    6155..6949 
                     /label="Kan/Neo"  
     misc_feature    6984..7286 
                     /label="SV40 ori"  
     misc_feature    7372..7849 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4053..4059 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4053 
                     /label="MAN2B1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGCCTAC GCGCGGGCTT CGGGGGTCTG CGCTCGCGGC TGCCTGGACT 
     1081 CAGCAGGCCC CTGGACCATG TCCCGCGCCC TGCGGCCACC GCTCCCGCCT CTCTGCTTTT 
     1141 TCCTTTTGTT GCTGGCGGCT GCCGGTGCTC GGGCCGGGGG ATACGAGACA TGCCCCACAG 
     1201 TGCAGCCGAA CATGCTGAAC GTGCACCTGC TGCCTCACAC ACATGATGAC GTGGGCTGGC 
     1261 TCAAAACCGT GGACCAGTAC TTTTATGGAA TCAAGAATGA CATCCAGCAC GCCGGTGTGC 
     1321 AGTACATCCT GGACTCGGTC ATCTCTGCCT TGCTGGCAGA TCCCACCCGT CGCTTCATTT 
     1381 ACGTGGAGAT TGCCTTCTTC TCCCGTTGGT GGCACCAGCA GACAAATGCC ACACAGGAAG 
     1441 TCGTGCGAGA CCTTGTGCGC CAGGGGCGCC TGGAGTTCGC CAATGGTGGC TGGGTGATGA 
     1501 ACGATGAGGC AGCCACCCAC TACGGTGCCA TCGTGGACCA GATGACACTT GGGCTGCGCT 
     1561 TTCTGGAGGA CACATTTGGC AATGATGGGC GACCCCGTGT GGCCTGGCAC ATTGACCCCT 
     1621 TCGGCCACTC TCGGGAGCAG GCCTCGCTGT TTGCGCAGAT GGGCTTCGAC GGCTTCTTCT 
     1681 TTGGGCGCCT TGATTATCAA GATAAGTGGG TACGGATGCA GAAGCTGGAG ATGGAGCAGG 
     1741 TGTGGCGGGC CAGCACCAGC CTGAAGCCCC CGACCGCGGA CCTCTTCACT GGTGTGCTTC 
     1801 CCAATGGTTA CAACCCGCCA AGGAATCTGT GCTGGGATGT GCTGTGTGTC GATCAGCCGC 
     1861 TGGTGGAGGA CCCTCGCAGC CCCGAGTACA ACGCCAAGGA GCTGGTCGAT TACTTCCTAA 
     1921 ATGTGGCCAC TGCCCAGGGC CGGTATTACC GCACCAACCA CACTGTGATG ACCATGGGCT 
     1981 CGGACTTCCA ATATGAGAAT GCCAACATGT GGTTCAAGAA CCTTGACAAG CTCATCCGGC 
     2041 TGGTAAATGC GCAGCAGGCA AAAGGAAGCA GTGTCCATGT TCTCTACTCC ACCCCCGCTT 
     2101 GTTACCTCTG GGAGCTGAAC AAGGCCAACC TCACCTGGTC AGTGAAACAT GACGACTTCT 
     2161 TCCCTTACGC GGATGGCCCC CACCAGTTCT GGACCGGTTA CTTTTCCAGT CGGCCGGCCC 
     2221 TCAAACGCTA CGAGCGCCTC AGCTACAACT TCCTGCAGGT GTGCAACCAG CTGGAGGCGC 
     2281 TGGTGGGCCT GGCGGCCAAC GTGGGACCCT ATGGCTCCGG AGACAGTGCA CCCCTCAATG 
     2341 AGGCGATGGC TGTGCTCCAG CATCACGACG CCGTCAGCGG CACCTCCCGC CAGCACGTGG 
     2401 CCAACGACTA CGCGCGCCAG CTTGCGGCAG GCTGGGGGCC TTGCGAGGTT CTTCTGAGCA 
     2461 ACGCGCTGGC GCGGCTCAGA GGCTTCAAAG ATCACTTCAC CTTTTGCCAA CAGCTAAACA 
     2521 TCAGCATCTG CCCGCTCAGC CAGACGGCGG CGCGCTTCCA GGTCATCGTT TATAATCCCC 
     2581 TGGGGCGGAA GGTGAATTGG ATGGTACGGC TGCCGGTCAG CGAAGGCGTT TTCGTTGTGA 
     2641 AGGACCCCAA TGGCAGGACA GTGCCCAGCG ATGTGGTAAT ATTTCCCAGC TCAGACAGCC 
     2701 AGGCGCACCC TCCGGAGCTG CTGTTCTCAG CCTCACTGCC CGCCCTGGGC TTCAGCACCT 
     2761 ATTCAGTAGC CCAGGTGCCT CGCTGGAAGC CCCAGGCCCG CGCACCACAG CCCATCCCCA 
     2821 GAAGATCCTG GTCCCCTGCT TTAACCATCG AAAATGAGCA CATCCGGGCA ACGTTTGATC 
     2881 CTGACACAGG GCTGTTGATG GAGATTATGA ACATGAATCA GCAACTCCTG CTGCCTGTTC 
     2941 GCCAGACCTT CTTCTGGTAC AACGCCAGTA TAGGTGACAA CGAAAGTGAC CAGGCCTCAG 
     3001 GTGCCTACAT CTTCAGACCC AACCAACAGA AACCGCTGCC TGTGAGCCGC TGGGCTCAGA 
     3061 TCCACCTGGT GAAGACACCC TTGGTGCAGG AGGTGCACCA GAACTTCTCA GCTTGGTGTT 
     3121 CCCAGGTGGT TCGCCTGTAC CCAGGACAGC GGCACCTGGA GCTAGAGTGG TCGGTGGGGC 
     3181 CGATACCTGT GGGCGACACC TGGGGGAAGG AGGTCATCAG CCGTTTTGAC ACACCGCTGG 
     3241 AGACAAAGGG ACGCTTCTAC ACAGACAGCA ATGGCCGGGA GATCCTGGAG AGGAGGCGGG 
     3301 ATTATCGACC CACCTGGAAA CTGAACCAGA CGGAGCCCGT GGCAGGAAAC TACTATCCAG 
     3361 TCAACACCCG GATTTACATC ACGGATGGAA ACATGCAGCT GACTGTGCTG ACTGACCGCT 
     3421 CCCAGGGGGG CAGCAGCCTG AGAGATGGCT CGCTGGAGCT CATGGTGCAC CGAAGGCTGC 
     3481 TGAAGGACGA TGGACGCGGA GTATCGGAGC CACTAATGGA GAACGGGTCG GGGGCGTGGG 
     3541 TGCGAGGGCG CCACCTGGTG CTGCTGGACA CAGCCCAGGC TGCAGCCGCC GGACACCGGC 
     3601 TCCTGGCGGA GCAGGAGGTC CTGGCCCCTC AGGTGGTGCT GGCCCCGGGT GGCGGCGCCG 
     3661 CCTACAATCT CGGGGCTCCT CCGCGCACGC AGTTCTCAGG GCTGCGCAGG GACCTGCCGC 
     3721 CCTCGGTGCA CCTGCTCACG CTGGCCAGCT GGGGCCCCGA AATGGTGCTG CTGCGCTTGG 
     3781 AGCACCAGTT TGCCGTAGGA GAGGATTCCG GACGTAACCT GAGCGCCCCC GTTACCTTGA 
     3841 ACTTGAGGGA CCTGTTCTCC ACCTTCACCA TCACCCGCCT GCAGGAGACC ACGCTGGTGG 
     3901 CCAACCAGCT CCGCGAGGCA GCCTCCAGGC TCAAGTGGAC AACAAACACA GGCCCCACAC 
     3961 CCCACCAAAC TCCGTACCAG CTGGACCCGG CCAACATCAC GCTGGAACCC ATGGAAATCC 
     4021 GCACTTTCCT GGCCTCAGTT CAATGGAAGG AGGTGGATGG TACGCGTACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//