LOCUS       RC200581L2                9057 bp    DNA     circular  SYN 
DEFINITION  PPP1R15A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A)
ACCESSION   NM_014330
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7348..7528 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8256..8915 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7576..8195 
                     /label="ColE1"  
     misc_feature    6223..6243 
                     /label="LR50"  
     misc_feature    5363..6088 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6232..6823 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5342..5348 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5342 
                     /label="PPP1R15A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCCCAGGC CAAGCACCCC ATCAGGCTAC 
     3361 CCCGTGGAGG GATGCCCACC CTTTCTTCCT CCTGTCCCCA GTGATGGGCC TCCTCAGCCG 
     3421 CGCCTGGAGC CGCCTGAGGG GCCTGGGACC TCTAGAGCCC TGGCTGGTGG AAGCAGTAAA 
     3481 AGGAGCAGCT CTGGTAGAAG CTGGCCTGGA GGGAGAAGCT AGGACTCCTC TGGCAATCCC 
     3541 CCATACCCCT TGGGGCAGAC GCCCTGAAGA GGAGGCTGAA GACAGTGGAG GCCCTGGAGA 
     3601 GGACAGAGAA ACACTGGGGC TGAAAACCAG CAGTTCCCTT CCTGAAGCCT GGGGACTTTT 
     3661 GGATGATGAT GATGGCATGT ATGGTGAGCG AGAGGCAACC AGTGTCCCTA GAGGGCAGGG 
     3721 AAGTCAATTT GCAGATGGCC AGCGTGCTCC CCTGTCTCCC AGCCTTCTGA TAAGGACACT 
     3781 GCAAGGTTCT GATAAGAACC CAGGGGAGGA GAAAGCCGAG GAAGAGGGAG TTGCTGAAGA 
     3841 GGAGGGAGTT AACAAGTTCT CTTATCCACC ATCACACCGG GAGTGTTGTC CAGCCGTGGA 
     3901 GGAGGAGGAC GATGAAGAAG CTGTAAAGAA AGAAGCTCAC AGAACCTCTA CTTCTGCCTT 
     3961 GTCTCCAGGA TCCAAGCCCA GCACTTGGGT GTCTTGCCCA GGGGAGGAAG AGAATCAAGC 
     4021 CACGGAGGAT AAAAGAACAG AAAGAAGTAA AGGAGCCAGG AAGACCTCCG TGTCCCCCCG 
     4081 ATCTTCAGGC TCCGACCCCA GGTCCTGGGA GTATCGTTCA GGAGAGGCGT CCGAGGAGAA 
     4141 GGAGGAAAAG GCACACAAAG AAACTGGGAA AGGAGAAGCT GCCCCAGGGC CGCAATCCTC 
     4201 AGCCCCAGCC CAGAGGCCCC AGCTCAAGTC CTGGTGGTGC CAACCCAGTG ATGAAGAGGA 
     4261 GGGTGAGGTC AAGGCTTTGG GGGCAGCTGA GAAGGATGGA GAAGCTGAGT GTCCTCCCTG 
     4321 CATCCCCCCA CCAAGTGCCT TCCTGAAGGC CTGGGTGTAT TGGCCAGGAG AGGACACAGA 
     4381 GGAAGAGGAA GATGAGGAAG AAGATGAGGA CAGTGACTCT GGATCAGATG AGGAAGAGGG 
     4441 AGAAGCTGAG GCTTCCTCTT CCACTCCTGC TACAGGTGTC TTCTTGAAGT CCTGGGTCTA 
     4501 TCAGCCAGGA GAGGACACAG AGGAGGAGGA AGATGAGGAC AGTGATACAG GATCAGCCGA 
     4561 GGATGAAAGA GAAGCTGAGA CTTCTGCTTC CACACCCCCT GCAAGTGCTT TCTTGAAGGC 
     4621 CTGGGTGTAT CGGCCAGGAG AGGACACGGA GGAGGAGGAA GATGAGGATG TGGATAGTGA 
     4681 GGATAAGGAA GATGATTCAG AAGCAGCCTT GGGAGAAGCT GAGTCAGACC CACATCCCTC 
     4741 CCACCCGGAC CAGAGGGCCC ACTTCAGGGG CTGGGGATAT CGACCTGGAA AAGAGACAGA 
     4801 GGAAGAGGAA GCTGCTGAGG ACTGGGGAGA AGCTGAGCCC TGCCCCTTCC GAGTGGCCAT 
     4861 CTATGTACCT GGAGAGAAGC CACCGCCTCC CTGGGCTCCT CCTAGGCTGC CCCTCCGACT 
     4921 GCAAAGGCGG CTCAAGCGCC CAGAAACCCC TACTCATGAT CCGGACCCTG AGACTCCCCT 
     4981 AAAGGCCAGA AAGGTGCGCT TCTCCGAGAA GGTCACTGTC CATTTCCTGG CTGTCTGGGC 
     5041 AGGGCCGGCC CAGGCCGCCC GCCAGGGCCC CTGGGAGCAG CTTGCTCGGG ATCGCAGCCG 
     5101 CTTCGCACGC CGCATCACCC AGGCCCAGGA GGAGCTGAGC CCCTGCCTCA CCCCTGCTGC 
     5161 CCGGGCCAGA GCCTGGGCAC GCCTCAGGAA CCCACCTTTA GCCCCCATCC CTGCCCTCAC 
     5221 CCAGACCTTG CCTTCCTCCT CTGTCCCTTC GTCCCCAGTC CAGACCACGC CCTTGAGCCA 
     5281 AGCTGTGGCC ACACCTTCCC GCTCGTCTGC TGCTGCAGCG GCTGCCCTGG ACCTCAGTGG 
     5341 GAGGCGTGGC ACGCGTACGC GGCCGCTCGA GATGAGCGGG GGCGAGGAGC TGTTCGCCGG 
     5401 CATCGTGCCC GTGCTGATCG AGCTGGACGG CGACGTGCAC GGCCACAAGT TCAGCGTGCG 
     5461 CGGCGAGGGC GAGGGCGACG CCGACTACGG CAAGCTGGAG ATCAAGTTCA TCTGCACCAC 
     5521 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT GGTGACCACC CTCTGCTACG GCATCCAGTG 
     5581 CTTCGCCCGC TACCCCGAGC ACATGAAGAT GAACGACTTC TTCAAGAGCG CCATGCCCGA 
     5641 GGGCTACATC CAGGAGCGCA CCATCCAGTT CCAGGACGAC GGCAAGTACA AGACCCGCGG 
     5701 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCAAGGACTT 
     5761 CAAGGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AGCTTCAACA GCCACAACGT 
     5821 GTACATCCGC CCCGACAAGG CCAACAACGG CCTGGAGGCT AACTTCAAGA CCCGCCACAA 
     5881 CATCGAGGGC GGCGGCGTGC AGCTGGCCGA CCACTACCAG ACCAACGTGC CCCTGGGCGA 
     5941 CGGCCCCGTG CTGATCCCCA TCAACCACTA CCTGAGCACT CAGACCAAGA TCAGCAAGGA 
     6001 CCGCAACGAG GCCCGCGACC ACATGGTGCT CCTGGAGTCC TTCAGCGCCT GCTGCCACAC 
     6061 CCACGGCATG GACGAGCTGT ACAGGTCCGG ACTCAGATAA GTTTAAACGG CCGGCCGCGG 
     6121 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6181 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6241 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6301 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6361 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6421 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     6481 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     6541 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     6601 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     6661 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     6721 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     6781 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     6841 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     6901 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     6961 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     7021 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7081 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7141 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7201 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7261 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7321 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7381 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7441 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     7501 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     7561 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     7621 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     7681 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     7741 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     7801 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     7861 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     7921 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     7981 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     8041 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8101 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8161 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8221 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8281 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8341 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8401 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     8461 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     8521 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     8581 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     8641 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     8701 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     8761 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     8821 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     8881 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     8941 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9001 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
//