LOCUS       RC200527                7704 bp    DNA     circular  SYN 
DEFINITION  PRPF6 (Myc-DDK-tagged)-Human PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6)
ACCESSION   NM_012469
VERSION     NM_012469.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3861..3952 
                     /label="Myc-DDK"  
     misc_feature    3982..4567 
                     /label="PolyA signal"  
     misc_feature    4039..4058 
                     /label="Restriction Enzyme"  
     misc_feature    4843..5515 
                     /label="ColE1"  
     misc_feature    5945..6739 
                     /label="Kan/Neo"  
     misc_feature    6774..7076 
                     /label="SV40 ori"  
     misc_feature    7162..7639 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3843..3849 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3843 
                     /label="PRPF6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACAAGAAG AAGAAACCGT TCCTAGGGAT GCCCGCGCCC CTCGGCTACG 
     1081 TGCCGGGGCT GGGCCGGGGC GCCACTGGCT TCACCACGCG GTCAGACATT GGGCCCGCCC 
     1141 GTGATGCAAA TGACCCTGTG GATGATCGCC ATGCACCCCC AGGCAAGAGA ACCGTTGGGG 
     1201 ACCAGATGAA GAAAAATCAG GCTGCTGACG ATGACGACGA GGATCTAAAT GACACCAATT 
     1261 ACGATGAGTT TAATGGCTAT GCTGGGAGCC TCTTCTCAAG TGGACCCTAC GAGAAAGATG 
     1321 ATGAGGAAGC AGATGCTATC TATGCAGCCC TGGATAAAAG GATGGATGAA AGAAGAAAAG 
     1381 AAAGACGGGA GCAAAGGGAG AAAGAAGAAA TAGAGAAATA TCGTATGGAA CGCCCCAAAA 
     1441 TCCAACAGCA GTTCTCAGAC CTCAAGAGGA AGTTGGCAGA AGTCACAGAA GAAGAGTGGC 
     1501 TGAGCATCCC CGAGGTTGGC GATGCCAGAA ATAAACGTCA GCGGAACCCA CGCTATGAGA 
     1561 AGCTGACCCC TGTTCCTGAC AGTTTCTTTG CCAAACATTT ACAGACCGGA GAGAACCATA 
     1621 CCTCAGTGGA TCCCCGACAA ACTCAATTTG GAGGTCTTAA CACACCCTAT CCAGGTGGAC 
     1681 TAAACACTCC ATACCCAGGT GGAATGACGC CAGGACTGAT GACACCTGGC ACAGGTGAGC 
     1741 TGGACATGAG GAAGATTGGC CAAGCGAGGA ACACTCTGAT GGACATGAGG CTGAGCCAGG 
     1801 TGTCTGACTC CGTGAGTGGA CAGACCGTCG TTGACCCCAA AGGCTACCTG ACGGATTTAA 
     1861 ATTCCATGAT CCCGACACAC GGAGGAGACA TCAATGATAT CAAGAAGGCG CGACTGCTCC 
     1921 TCAAGTCTGT TCGGGAGACG AACCCTCATC ACCCGCCAGC CTGGATTGCA TCAGCCCGCC 
     1981 TGGAAGAAGT CACTGGGAAG CTACAAGTAG CTCGGAACCT TATCATGAAG GGGACGGAGA 
     2041 TGTGCCCCAA GAGTGAAGAT GTCTGGCTGG AAGCAGCCAG GTTGCAGCCT GGGGACACAG 
     2101 CCAAGGCCGT GGTAGCCCAA GCTGTCCGTC ATCTCCCACA GTCTGTCAGG ATTTACATCA 
     2161 GAGCCGCAGA GCTGGAAACG GACATTCGTG CAAAGAAGCG GGTTCTTCGG AAAGCCCTCG 
     2221 AGCATGTTCC AAACTCGGTT CGCTTGTGGA AAGCAGCCGT TGAGCTGGAA GAACCTGAAG 
     2281 ATGCTAGAAT CATGCTGAGC CGAGCTGTGG AGTGCTGCCC CACCAGCGTG GAGCTCTGGC 
     2341 TTGCTCTGGC AAGGCTGGAG ACCTATGAAA ATGCCCGCAA GGTCTTGAAC AAGGCGCGGG 
     2401 AGAACATTCC TACAGACCGA CATATCTGGA TCACGGCTGC TAAGCTGGAG GAAGCCAATG 
     2461 GGAACACGCA GATGGTGGAG AAGATCATCG ACCGAGCCAT CACCTCGCTG CGGGCCAACG 
     2521 GTGTGGAGAT CAACCGTGAG CAGTGGATCC AGGATGCCGA GGAATGTGAC AGGGCTGGGA 
     2581 GTGTGGCCAC CTGCCAGGCC GTCATGCGTG CCGTGATTGG GATTGGGATT GAGGAGGAAG 
     2641 ATCGGAAGCA TACCTGGATG GAGGATGCTG ACAGTTGTGT AGCCCACAAT GCCCTGGAGT 
     2701 GTGCACGAGC CATCTACGCC TACGCCCTGC AGGTGTTCCC CAGCAAGAAG AGTGTGTGGC 
     2761 TGCGCGCCGC GTACTTCGAG AAGAACCATG GCACTCGGGA GTCCCTGGAA GCACTCCTGC 
     2821 AGAGGGCTGT GGCCCACTGC CCCAAAGCAG AGGTGCTGTG GCTCATGGGC GCCAAGTCCA 
     2881 AGTGGCTGGC AGGGGATGTG CCTGCAGCAA GGAGCATCCT GGCCCTGGCC TTCCAGGCCA 
     2941 ACCCCAACAG TGAGGAGATC TGGCTGGCAG CCGTGAAGCT GGAGTCCGAG AATGATGAGT 
     3001 ACGAGCGGGC CCGGAGGCTG CTGGCCAAGG CGCGGAGCAG TGCCCCCACC GCCCGGGTGT 
     3061 TCATGAAGTC TGTGAAGCTG GAGTGGGTGC AAGACAACAT CAGGGCAGCC CAAGATCTGT 
     3121 GCGAGGAGGC CCTGCGGCAC TATGAGGACT TCCCCAAGCT GTGGATGATG AAGGGGCAGA 
     3181 TCGAGGAGCA GAAGGAGATG ATGGAGAAGG CGCGGGAAGC CTATAACCAG GGGTTGAAGA 
     3241 AGTGTCCCCA CTCCACACCC CTGTGGCTTT TGCTCTCTCG GCTGGAGGAG AAGATTGGGC 
     3301 AGCTTACTCG AGCACGGGCC ATTTTGGAAA AGTCTCGTCT GAAGAACCCA AAGAACCCTG 
     3361 GGCTGTGGTT GGAGTCCGTG CGGCTGGAGT ACCGTGCGGG GCTGAAGAAC ATCGCAAATA 
     3421 CACTCATGGC CAAGGCGCTG CAGGAGTGCC CCAACTCCGG TATCCTGTGG TCTGAGGCCA 
     3481 TCTTCCTCGA GGCAAGGCCC CAGAGGAGGA CCAAGAGCGT GGATGCCCTG AAGAAGTGTG 
     3541 AGCATGACCC CCATGTGCTC CTGGCCGTGG CCAAGCTGTT TTGGAGTCAG CGGAAGATCA 
     3601 CCAAGGCCAG GGAGTGGTTC CACCGCACTG TGAAGATTGA CTCGGACCTG GGGGATGCCT 
     3661 GGGCCTTCTT CTACAAGTTT GAGCTGCAGC ATGGCACTGA GGAGCAGCAG GAGGAGGTGA 
     3721 GGAAGCGCTG TGAGAGTGCA GAGCCTCGGC ATGGGGAGCT GTGGTGCGCC GTGTCCAAGG 
     3781 ACATCGCCAA CTGGCAGAAG AAGATCGGGG ACATCCTTAG GCTGGTGGCC GGCCGCATCA 
     3841 AGAACACCTT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3901 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3961 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4021 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4081 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4141 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4201 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4261 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4321 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4381 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4441 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4501 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4561 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4621 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4681 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4741 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4801 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4861 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4921 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4981 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5041 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5101 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5161 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5221 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5281 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5341 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5401 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5461 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5521 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5581 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5641 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5701 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5761 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5821 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5881 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5941 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6001 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6061 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6121 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6181 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6241 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6301 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6361 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6421 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6481 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6541 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6601 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6661 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6721 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6781 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6841 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6901 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6961 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7021 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7081 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7141 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7201 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7261 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7321 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7381 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7441 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7501 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7561 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7621 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7681 TAACAAAAAT TTAACGCGAA TTTT
//