LOCUS       RC200445L1                8934 bp    DNA     circular  SYN 
DEFINITION  PDE6B (Myc-DDK-tagged)-Human phosphodiesterase 6B, cGMP-specific, rod, beta (PDE6B), transcript variant 1
ACCESSION   NM_000283
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7225..7405 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8133..8792 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7564..8132 
                     /label="ColE1"  
     misc_feature    6100..6120 
                     /label="LR50 primer"  
     misc_feature    5897..5968 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6109..6700 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5879..5885 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5879 
                     /label="PDE6B"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGCCTCAGT GAGGAGCAGG CCCGGAGCTT 
     3361 TCTGGACCAG AACCCCGATT TTGCCCGCCA GTACTTTGGG AAGAAACTGA GCCCTGAGAA 
     3421 TGTGGCCGCG GCCTGCGAGG ACGGGTGCCC GCCGGACTGC GACAGCCTCC GGGACCTCTG 
     3481 CCAGGTGGAG GAGAGCACGG CGCTGCTGGA GCTGGTGCAG GATATGCAGG AGAGCATCAA 
     3541 CATGGAGCGC GTGGTCTTCA AGGTCCTGCG GCGCCTCTGC ACCCTCCTGC AGGCCGACCG 
     3601 CTGCAGCCTC TTCATGTACC GCCAGCGCAA CGGCGTGGCC GAGCTGGCCA CCAGGCTTTT 
     3661 CAGCGTGCAG CCGGACAGCG TCCTGGAGGA CTGCCTGGTG CCCCCCGACT CCGAGATCGT 
     3721 CTTCCCACTG GACATCGGGG TCGTGGGCCA CGTGGCTCAG ACCAAAAAGA TGGTGAACGT 
     3781 CGAGGACGTG GCCGAGTGCC CTCACTTCAG CTCATTTGCT GACGAGCTCA CTGACTACAA 
     3841 GACAAAGAAT ATGCTGGCCA CACCCATCAT GAATGGCAAA GACGTCGTGG CGGTGATCAT 
     3901 GGCAGTGAAC AAGCTCAACG GCCCATTCTT CACCAGCGAA GACGAAGATG TGTTCTTGAA 
     3961 GTACCTGAAT TTTGCCACGT TGTACCTGAA GATCTATCAC CTGAGCTACC TCCACAACTG 
     4021 CGAGACGCGC CGCGGCCAGG TGCTGCTGTG GTCGGCCAAC AAGGTGTTTG AGGAGCTGAC 
     4081 GGACATCGAG AGGCAGTTCC ACAAGGCCTT CTACACGGTG CGGGCCTACC TCAACTGCGA 
     4141 GCGGTACTCC GTGGGCCTCC TGGACATGAC CAAGGAGAAG GAATTTTTTG ACGTGTGGTC 
     4201 TGTGCTGATG GGAGAGTCCC AGCCGTACTC GGGCCCACGC ACGCCTGATG GCCGGGAAAT 
     4261 TGTCTTCTAC AAAGTGATCG ACTACATCCT CCACGGCAAG GAGGAGATCA AGGTCATTCC 
     4321 CACACCCTCA GCCGATCACT GGGCCCTGGC CAGCGGCCTT CCAAGCTACG TGGCAGAAAG 
     4381 CGGCTTTATT TGTAACATCA TGAATGCTTC CGCTGACGAA ATGTTCAAAT TTCAGGAAGG 
     4441 GGCCCTGGAC GACTCCGGGT GGCTCATCAA GAATGTGCTG TCCATGCCCA TCGTCAACAA 
     4501 GAAGGAGGAG ATTGTGGGAG TCGCCACATT TTACAACAGG AAAGACGGGA AGCCCTTTGA 
     4561 CGAACAGGAC GAGGTTCTCA TGGAGTCCCT GACACAGTTC CTGGGCTGGT CAGTGATGAA 
     4621 CACCGACACC TACGACAAGA TGAACAAGCT GGAGAACCGC AAGGACATCG CACAGGACAT 
     4681 GGTCCTTTAC CACGTGAAGT GCGACAGGGA CGAGATCCAG CTCATCCTGC CAACCAGAGC 
     4741 GCGCCTGGGG AAGGAGCCTG CTGACTGCGA TGAGGACGAG CTGGGCGAAA TCCTGAAGGA 
     4801 GGAGCTGCCA GGGCCCACCA CATTTGACAT CTACGAATTC CACTTCTCTG ACCTGGAGTG 
     4861 CACCGAACTG GACCTGGTCA AATGTGGCAT CCAGATGTAC TACGAGCTGG GCGTGGTCCG 
     4921 AAAGTTCCAG ATCCCCCAGG AGGTCCTGGT GCGGTTCCTG TTCTCCATCA GCAAAGGGTA 
     4981 CCGGAGAATC ACCTACCACA ACTGGCGCCA CGGCTTCAAC GTGGCCCAGA CGATGTTCAC 
     5041 GCTGCTCATG ACCGGCAAAC TGAAGAGCTA CTACACGGAC CTGGAGGCCT TCGCCATGGT 
     5101 GACAGCCGGC CTGTGCCATG ACATCGACCA CCGCGGCACC AACAACCTGT ACCAGATGAA 
     5161 GTCCCAGAAC CCCTTGGCTA AGCTCCACGG CTCCTCGATT TTGGAGCGGC ACCACCTGGA 
     5221 GTTTGGGAAG TTCCTGCTCT CGGAGGAGAC CCTGAACATC TACCAGAACC TGAACCGGCG 
     5281 GCAGCACGAT CACGTGATCC ACCTGATGGA CATCGCCATC ATCGCCACGG ACCTGGCCCT 
     5341 GTACTTCAAG AAGAGAGCGA TGTTTCAGAA GATCGTGGAT GAGTCCAAGA ACTACCAGGA 
     5401 CAAGAAGAGC TGGGTGGAGT ACCTGTCCCT GGAGACGACC CGGAAGGAGA TCGTCATGGC 
     5461 CATGATGATG ACAGCCTGCG ACCTGTCTGC CATCACCAAG CCCTGGGAAG TCCAGAGCAA 
     5521 GGTCGCACTT CTCGTGGCTG CTGAGTTCTG GGAGCAAGGT GACTTGGAAA GGACAGTCTT 
     5581 GGATCAGCAG CCCATTCCTA TGATGGACCG GAACAAGGCG GCCGAGCTCC CCAAGCTGCA 
     5641 AGTGGGCTTC ATCGACTTCG TGTGCACATT CGTGTACAAG GAGTTCTCTC GTTTCCACGA 
     5701 AGAGATCCTG CCCATGTTCG ACCGACTGCA GAACAATAGG AAAGAGTGGA AGGCGCTGGC 
     5761 TGATGAGTAT GAGGCCAAAG TGAAGGCTCT GGAGGAGAAG GAGGAGGAGG AGAGGGTGGC 
     5821 AGCCAAGAAA GGCACAGAAA TTTGCAATGG CGGCCCAGCA CCCAAGTCTT CAACCTGCTG 
     5881 TATCCTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC 
     5941 AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCT 
     6001 GTACAAGTAG GATTCGTCGA GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT 
     6061 ATACATGTTT AAGGGTTCCG GTTCCACTAG GTACAATTCG ATATCAAGCT TATCGATAAT 
     6121 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT 
     6181 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG 
     6241 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG 
     6301 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT 
     6361 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT 
     6421 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG 
     6481 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC 
     6541 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT 
     6601 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC 
     6661 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA 
     6721 CCTCGATCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA 
     6781 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC 
     6841 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA 
     6901 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT 
     6961 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG 
     7021 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA 
     7081 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG 
     7141 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA 
     7201 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 
     7261 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 
     7321 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 
     7381 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGCATG TGAGCAAAAG GCCAGCAAAA 
     7441 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     7501 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     7561 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     7621 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     7681 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     7741 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     7801 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     7861 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     7921 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     7981 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     8041 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     8101 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG ATTACGCCCC GCCCTGCCAC 
     8161 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 
     8221 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 
     8281 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG 
     8341 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG 
     8401 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC 
     8461 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA 
     8521 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA 
     8581 CGGAACTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 
     8641 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 
     8701 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 
     8761 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TACTCTTCCT TTTTCAATAT 
     8821 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8881 AAAAATAAAC AAATAGGGGT CCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAC
//