LOCUS       RC200442                8676 bp    DNA     circular  SYN 
DEFINITION  PLCG2 (Myc-DDK-tagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)
ACCESSION   NM_002661
VERSION     NM_002661.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4833..4924 
                     /label="Myc-DDK"  
     misc_feature    4954..5539 
                     /label="PolyA signal"  
     misc_feature    5011..5030 
                     /label="Restriction Enzyme"  
     misc_feature    5815..6487 
                     /label="ColE1"  
     misc_feature    6917..7711 
                     /label="Kan/Neo"  
     misc_feature    7746..8048 
                     /label="SV40 ori"  
     misc_feature    8134..8611 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4821 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4815 
                     /label="PLCG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCACCACG GTCAATGTAG ATTCCCTTGC GGAATATGAG AAGAGCCAGA 
     1081 TCAAGAGAGC CCTGGAGCTG GGGACGGTGA TGACTGTGTT CAGCTTCCGC AAGTCCACCC 
     1141 CCGAGCGGAG AACCGTCCAG GTGATCATGG AGACGCGGCA GGTGGCCTGG AGCAAGACCG 
     1201 CCGACAAGAT CGAGGGCTTC TTGGATATCA TGGAAATAAA AGAAATCCGC CCAGGGAAGA 
     1261 ACTCCAAAGA TTTCGAGCGA GCAAAAGCAG TTCGCCAGAA AGAAGACTGC TGCTTCACCA 
     1321 TCCTATATGG CACTCAGTTC GTCCTCAGCA CGCTCAGCTT GGCAGCTGAC TCTAAAGAGG 
     1381 ATGCAGTTAA CTGGCTCTCT GGCTTGAAAA TCTTACACCA GGAAGCGATG AATGCGTCCA 
     1441 CGCCCACCAT TATCGAGAGT TGGCTGAGAA AGCAGATATA TTCTGTGGAT CAAACCAGAA 
     1501 GAAACAGCAT CAGTCTCCGA GAGTTGAAGA CCATCTTGCC CCTGATCAAC TTTAAAGTGA 
     1561 GCAGTGCCAA GTTCCTTAAA GATAAGTTTG TGGAAATAGG AGCACACAAA GATGAGCTCA 
     1621 GCTTTGAACA GTTCCATCTC TTCTATAAAA AACTTATGTT TGAACAGCAA AAATCGATTC 
     1681 TCGATGAATT CAAAAAGGAT TCGTCCGTGT TCATCCTGGG GAACACTGAC AGGCCGGATG 
     1741 CCTCTGCTGT TTACCTGCGT GACTTCCAGA GGTTTCTCAT ACATGAACAG CAGGAGCATT 
     1801 GGGCTCAGGA TCTGAACAAA GTCCGTGAGC GGATGACAAA GTTCATTGAT GACACCATGC 
     1861 GTGAAACTGC TGAGCCTTTC TTGTTTGTGG ATGAGTTCCT CACGTACCTG TTTTCACGAG 
     1921 AAAACAGCAT CTGGGATGAG AAGTATGACG CGGTGGACAT GCAGGACATG AACAACCCCC 
     1981 TGTCTCATTA CTGGATCTCC TCGTCACATA ACACGTACCT TACAGGTGAC CAGCTGCGGA 
     2041 GCGAGTCGTC CCCAGAAGCT TACATCCGCT GCCTGCGCAT GGGCTGTCGC TGCATTGAAC 
     2101 TGGACTGCTG GGACGGGCCC GATGGGAAGC CGGTCATCTA CCATGGCTGG ACGCGGACTA 
     2161 CCAAGATCAA GTTTGACGAC GTCGTGCAGG CCATCAAAGA CCACGCCTTT GTTACGTCGA 
     2221 GCTTCCCAGT GATCCTGTCC ATCGAGGAGC ACTGCAGCGT GGAGCAACAG CGTCACATGG 
     2281 CCAAGGCCTT CAAGGAAGTA TTTGGCGACC TGCTGTTGAC GAAGCCCACG GAGGCCAGTG 
     2341 CTGACCAGCT GCCCTCGCCC AGCCAGCTGC GGGAGAAGAT CATCATCAAG CATAAGAAGC 
     2401 TGGGCCCCCG AGGCGATGTG GATGTCAACA TGGAGGACAA GAAGGACGAA CACAAGCAAC 
     2461 AGGGGGAGCT GTACATGTGG GATTCCATTG ACCAGAAATG GACTCGGCAC TACTGCGCCA 
     2521 TTGCCGATGC CAAGCTGTCC TTCAGTGATG ACATTGAACA GACTATGGAG GAGGAAGTGC 
     2581 CCCAGGATAT ACCCCCTACA GAACTACATT TTGGGGAGAA ATGGTTCCAC AAGAAGGTGG 
     2641 AGAAGAGGAC GAGTGCCGAG AAGTTGCTGC AGGAATACTG CATGGAGACG GGGGGCAAGG 
     2701 ATGGCACCTT CCTGGTTCGG GAGAGCGAGA CCTTCCCCAA TGACTACACC CTGTCCTTCT 
     2761 GGCGGTCAGG CCGGGTCCAG CACTGCCGGA TCCGCTCCAC CATGGAGGGC GGGACCCTGA 
     2821 AATACTACTT GACTGACAAC CTCACCTTCA GCAGCATCTA TGCCCTCATC CAGCACTACC 
     2881 GCGAGACGCA CCTGCGCTGC GCCGAGTTCG AGCTGCGGCT CACGGACCCT GTGCCCAACC 
     2941 CCAACCCCCA CGAGTCCAAG CCGTGGTACT ATGACAGCCT GAGCCGCGGA GAGGCAGAGG 
     3001 ACATGCTGAT GAGGATTCCC CGGGACGGGG CCTTCCTGAT CCGGAAGCGA GAGGGGAGCG 
     3061 ACTCCTATGC CATCACCTTC AGGGCTAGGG GCAAGGTAAA GCATTGTCGC ATCAACCGGG 
     3121 ACGGCCGGCA CTTTGTGCTG GGGACCTCCG CCTATTTTGA GAGTCTGGTG GAGCTCGTCA 
     3181 GTTACTACGA GAAGCATTCA CTCTACCGAA AGATGAGACT GCGCTACCCC GTGACCCCCG 
     3241 AGCTCCTGGA GCGCTACAAT ATGGAAAGAG ATATAAACTC CCTCTACGAC GTCAGCAGAA 
     3301 TGTATGTGGA TCCCAGTGAA ATCAATCCGT CCATGCCTCA GAGAACCGTG AAAGCTCTGT 
     3361 ATGACTACAA AGCCAAGCGA AGCGATGAGC TGAGCTTCTG CCGTGGTGCC CTCATCCACA 
     3421 ATGTCTCCAA GGAGCCCGGG GGCTGGTGGA AAGGAGACTA TGGAACCAGG ATCCAGCAGT 
     3481 ACTTCCCATC CAACTACGTC GAGGACATCT CAACTGCAGA CTTCGAGGAG CTAGAAAAGC 
     3541 AGATTATTGA AGACAATCCC TTAGGGTCTC TTTGCAGAGG AATATTGGAC CTCAATACCT 
     3601 ATAACGTCGT GAAAGCCCCT CAGGGAAAAA ACCAGAAGTC CTTTGTCTTC ATCCTGGAGC 
     3661 CCAAGCAGCA GGGCTATCCT CCGGTGGAGT TTGCCACAGA CAGGGTGGAG GAGCTCTTTG 
     3721 AGTGGTTTCA GAGCATCCGA GAGATCACCT GGAAGATTGA CACCAAGGAG AACAACATGA 
     3781 AGTACTGGGA GAAGAACCAG TCCATCGCCA TCGAGCTCTC TGACCTGGTT GTCTACTGCA 
     3841 AACCAACCAG CAAAACCAAG GACAACTTAG AAAATCCTGA CTTCCGAGAA ATCCGCTCCT 
     3901 TTGTGGAGAC GAAGGCTGAC AGCATCATCA GACAGAAGCC CGTCGACCTC CTGAAGTACA 
     3961 ATCAAAAGGG CCTGACCCGC GTCTACCCAA AGGGACAAAG AGTTGACTCT TCAAACTACG 
     4021 ACCCCTTCCG CCTCTGGCTG TGCGGTTCTC AGATGGTGGC ACTCAATTTC CAGACGGCAG 
     4081 ATAAGTACAT GCAGATGAAT CACGCATTGT TTTCTCTCAA CGGGCGCACG GGCTACGTTC 
     4141 TGCAGCCTGA GAGCATGAGG ACAGAGAAAT ATGACCCGAT GCCACCCGAG TCCCAGAGGA 
     4201 AGATCCTGAT GACGCTGACA GTCAAGGTTC TCGGTGCTCG CCATCTCCCC AAACTTGGAC 
     4261 GAAGTATTGC CTGTCCCTTT GTAGAAGTGG AGATCTGTGG AGCCGAGTAT GACAACAACA 
     4321 AGTTCAAGAC GACGGTTGTG AATGATAATG GCCTCAGCCC TATCTGGGCT CCAACACAGG 
     4381 AGAAGGTGAC ATTTGAAATT TATGACCCAA ACCTGGCATT TCTGCGCTTT GTGGTTTATG 
     4441 AAGAAGATAT GTTCAGCGAT CCCAACTTTC TTGCTCATGC CACTTACCCC ATTAAAGCAG 
     4501 TCAAATCAGG ATTCAGGTCC GTTCCTCTGA AGAATGGGTA CAGCGAGGAC ATAGAGCTGG 
     4561 CTTCCCTCCT GGTTTTCTGT GAGATGCGGC CAGTCCTGGA GAGCGAAGAG GAACTTTACT 
     4621 CCTCCTGTCG CCAGCTGAGG AGGCGGCAAG AAGAACTGAA CAACCAGCTC TTTCTGTATG 
     4681 ACACACACCA GAACTTGCGC AATGCCAACC GGGATGCCCT GGTTAAAGAG TTCAGTGTTA 
     4741 ATGAGAACCA GCTCCAGCTG TACCAGGAGA AATGCAACAA GAGGTTAAGA GAGAAGAGAG 
     4801 TCAGCAACAG CAAGTTTTAC TCAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//