LOCUS       RC200440                8403 bp    DNA     circular  SYN 
DEFINITION  POLR2B (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B)
ACCESSION   NM_000938
VERSION     NM_000938.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4560..4651 
                     /label="Myc-DDK"  
     misc_feature    4681..5266 
                     /label="PolyA signal"  
     misc_feature    4738..4757 
                     /label="Restriction Enzyme"  
     misc_feature    5542..6214 
                     /label="ColE1"  
     misc_feature    6644..7438 
                     /label="Kan/Neo"  
     misc_feature    7473..7775 
                     /label="SV40 ori"  
     misc_feature    7861..8338 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4542..4548 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4542 
                     /label="POLR2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACGACGCG GATGAGGATA TGCAATATGA TGAGGATGAT GATGAAATCA 
     1081 CCCCGGATTT GTGGCAAGAA GCATGCTGGA TTGTAATCAG TTCCTATTTT GACGAGAAAG 
     1141 GCTTGGTTAG ACAACAGCTG GATTCTTTTG ATGAGTTTAT TCAGATGTCT GTTCAAAGAA 
     1201 TTGTGGAAGA CGCTCCTCCT ATAGACCTAC AGGCTGAAGC TCAGCATGCT AGTGGAGAAG 
     1261 TTGAAGAACC GCCACGATAT TTGCTGAAGT TTGAACAAAT TTATCTTTCC AAGCCTACCC 
     1321 ATTGGGAAAG AGATGGTGCT CCTTCACCAA TGATGCCCAA TGAAGCTAGA TTAAGGAATC 
     1381 TCACGTATTC TGCTCCGCTT TATGTTGATA TAACAAAAAC AGTCATTAAA GAAGGTGAAG 
     1441 AACAACTTCA GACTCAGCAT CAGAAAACTT TTATAGGAAA AATTCCAATT ATGTTGCGGT 
     1501 CAACTTACTG CCTTTTGAAT GGCTTGACAG ATCGTGATCT TTGTGAGTTA AATGAATGCC 
     1561 CTTTGGATCC TGGTGGCTAT TTCATTATTA ATGGATCAGA AAAGGTTCTG ATTGCCCAAG 
     1621 AGAAAATGGC AACAAACACA GTTTATGTGT TTGCCAAAAA GGATTCTAAA TATGCCTACA 
     1681 CAGGAGAGTG TAGATCATGT CTTGAGAATT CTTCCCGACC CACCAGTACT ATATGGGTTA 
     1741 GCATGCTGGC AAGAGGAGGA CAGGGTGCCA AGAAGAGTGC TATTGGTCAG CGCATTGTGG 
     1801 CAACTCTACC ATATATCAAG CAAGAAGTTC CCATCATTAT TGTGTTCAGA GCATTAGGTT 
     1861 TTGTGTCCGA CAGAGATATT TTAGAACATA TTATTTATGA TTTTGAAGAT CCAGAGATGA 
     1921 TGGAAATGGT TAAACCTTCT CTCGATGAAG CTTTTGTCAT CCAAGAACAG AATGTTGCAC 
     1981 TAAATTTCAT TGGTTCACGA GGAGCAAAGC CTGGTGTTAC TAAAGAGAAA AGAATTAAAT 
     2041 ATGCAAAGGA AGTTTTACAA AAAGAAATGC TCCCTCATGT TGGTGTCAGT GATTTTTGTG 
     2101 AGACCAAAAA AGCCTATTTC TTGGGATACA TGGTTCATAG GTTACTTCTG GCAGCTTTGG 
     2161 GTAGAAGAGA ACTAGATGAC AGAGATCACT ATGGAAACAA GAGATTGGAT CTTGCTGGGC 
     2221 CGCTGCTTGC ATTCTTATTT AGAGGTATGT TTAAGAATTT GCTTAAAGAA GTGCGGATCT 
     2281 ATGCACAGAA ATTTATTGAT CGAGGAAAGG ATTTTAACTT GGAGTTGGCA ATTAAAACAC 
     2341 GGATCATATC TGATGGCCTA AAATACTCTT TAGCTACTGG AAACTGGGGT GATCAAAAGA 
     2401 AAGCTCATCA AGCCAGAGCT GGAGTATCTC AGGTGTTAAA CCGCCTGACT TTTGCGTCTA 
     2461 CTCTTTCTCA CCTGCGTCGT TTAAATTCTC CTATTGGTAG AGACGGCAAG CTAGCAAAAC 
     2521 CAAGACAGTT GCATAATACG TTGTGGGGAA TGGTGTGTCC TGCCGAGACC CCAGAGGGCC 
     2581 ATGCTGTAGG ACTTGTGAAG AATTTAGCCT TGATGGCGTA TATTTCAGTT GGATCTCAAC 
     2641 CATCTCCAAT TCTGGAATTT TTAGAAGAAT GGAGTATGGA AAATTTAGAA GAAATTTCTC 
     2701 CTGCAGCTAT TGCTGATGCA ACCAAGATTT TTGTTAATGG CTGCTGGGTT GGAATACATA 
     2761 AAGATCCCGA ACAACTTATG AACACCCTAA GGAAATTGAG ACGTCAGATG GACATCATTG 
     2821 TGTCTGAAGT TTCTATGATC AGAGATATTC GAGAGAGGGA GATTCGGATC TATACGGATG 
     2881 CAGGCCGTAT TTGTAGACCA CTTCTGATTG TGGAAAAACA AAAGCTACTT TTGAAGAAGA 
     2941 GGCATATTGA CCAATTGAAA GAGAGAGAAT ATAACAACTA TAGTTGGCAG GATCTTGTGG 
     3001 CCAGTGGGGT AGTGGAGTAT ATTGATACCC TGGAAGAAGA AACAGTGATG CTTGCAATGA 
     3061 CTCCAGATGA TTTACAGGAG AAAGAAGTAG CTTATTGTTC CACATATACA CACTGTGAGA 
     3121 TTCATCCCTC AATGATCCTT GGTGTCTGTG CATCTATTAT TCCCTTTCCT GATCATAACC 
     3181 AGTCCCCTAG AAACACATAC CAGTCTGCTA TGGGTAAGCA GGCTATGGGA GTTTACATCA 
     3241 CCAACTTCCA TGTTCGCATG GACACATTGG CCCATGTTCT CTATTATCCT CAAAAGCCAC 
     3301 TTGTGACTAC ACGGTCTATG GAATATCTAC GATTTAGAGA GCTGCCAGCA GGCATCAACT 
     3361 CAATTGTGGC CATTGCATCA TACACTGGAT ATAATCAGGA AGACTCTGTT ATCATGAATC 
     3421 GTTCAGCTGT AGACCGCGGC TTCTTCAGGT CTGTTTTCTA TCGCTCATAC AAAGAACAGG 
     3481 AGTCTAAAAA AGGATTTGAT CAAGAAGAAG TTTTTGAGAA GCCTACACGT GAAACATGCC 
     3541 AGGGCATGAG GCATGCCATT TACGACAAGC TGGATGATGA TGGTTTGATA GCTCCAGGGG 
     3601 TTCGTGTATC AGGAGATGAT GTTATTATAG GCAAAACAGT CACCTTGCCT GAAAATGAAG 
     3661 ATGAATTGGA GAGCACCAAT AGACGCTATA CCAAGAGAGA CTGTAGCACT TTTCTCAGAA 
     3721 CTAGTGAGAC GGGCATTGTG GATCAGGTTA TGGTAACTCT CAATCAGGAA GGATATAAAT 
     3781 TTTGTAAAAT AAGGGTACGC TCTGTTAGGA TTCCACAGAT TGGAGACAAA TTTGCTAGTC 
     3841 GACATGGTCA AAAGGGTACT TGTGGTATTC AGTATAGACA AGAGGATATG CCTTTCACCT 
     3901 GTGAAGGTAT CACCCCTGAT ATCATCATCA ATCCCCATGC CATCCCCTCT CGTATGACTA 
     3961 TTGGTCACTT AATTGAATGC CTTCAAGGGA AGGTATCGGC TAACAAGGGT GAAATTGGTG 
     4021 ATGCCACTCC ATTTAATGAT GCTGTTAACG TGCAGAAGAT TTCTAATCTT TTATCTGATT 
     4081 ATGGCTATCA TCTCAGAGGA AATGAGGTCC TGTACAATGG GTTCACTGGT CGAAAAATCA 
     4141 CATCACAAAT ATTTATTGGC CCCACTTATT ACCAGCGTTT GAAGCATATG GTGGATGATA 
     4201 AGATTCACTC TCGTGCTAGG GGACCTATTC AGATCCTCAA TAGACAGCCC ATGGAGGGTA 
     4261 GATCTCGTGA TGGTGGCCTG CGTTTTGGAG AAATGGAACG AGATTGTCAG ATTGCCCATG 
     4321 GAGCAGCCCA GTTTTTAAGG GAAAGATTGT TTGAGGCATC AGATCCATAT CAGGTTCATG 
     4381 TTTGCAATCT TTGTGGAATA ATGGCGATTG CCAACACCAG GACCCATACA TATGAATGCA 
     4441 GGGGCTGCCG CAATAAAACC CAGATTTCTT TGGTGCGAAT GCCTTACGCA TGCAAACTAT 
     4501 TGTTTCAGGA ACTTATGTCT ATGAGTATTG CACCGCGAAT GATGAGTGTT ACGCGTACGC 
     4561 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4621 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4681 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4741 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4801 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4861 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4921 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4981 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5041 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5101 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5161 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5221 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5281 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5641 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5881 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6181 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6241 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6301 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6361 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6421 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6481 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6541 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6601 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6661 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6721 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6781 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6841 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6901 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6961 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7021 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7081 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7141 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7201 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7261 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7321 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7381 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7441 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7501 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7561 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7621 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7801 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7981 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8041 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8101 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8161 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8221 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8281 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8341 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8401 TTT
//