LOCUS       RC200421                7170 bp    DNA     circular  SYN 
DEFINITION  SATB1 (Myc-DDK-tagged)-Human SATB homeobox 1 (SATB1), transcript variant 1
ACCESSION   NM_002971
VERSION     NM_002971.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3327..3418 
                     /label="Myc-DDK"  
     misc_feature    3448..4033 
                     /label="PolyA signal"  
     misc_feature    3505..3524 
                     /label="Restriction Enzyme"  
     misc_feature    4309..4981 
                     /label="ColE1"  
     misc_feature    5411..6205 
                     /label="Kan/Neo"  
     misc_feature    6240..6542 
                     /label="SV40 ori"  
     misc_feature    6628..7105 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3309..3315 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3309 
                     /label="SATB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCATTTG AACGAGGCAA CTCAGGGGAA AGAACATTCA GAAATGTCTA 
     1081 ACAATGTGAG TGATCCGAAG GGTCCACCAG CCAAGATTGC CCGCCTGGAG CAGAACGGGA 
     1141 GCCCGCTAGG AAGAGGAAGG CTTGGGAGTA CAGGTGCAAA AATGCAGGGA GTGCCTTTAA 
     1201 AACACTCGGG CCATCTGATG AAAACCAACC TTAGGAAAGG AACCATGCTG CCAGTTTTCT 
     1261 GTGTGGTGGA ACATTATGAA AACGCCATTG AATATGATTG CAAGGAGGAG CATGCAGAAT 
     1321 TTGTGCTGGT GAGAAAGGAT ATGCTTTTCA ACCAGCTGAT CGAAATGGCA TTGCTGTCTC 
     1381 TAGGTTATTC ACATAGCTCT GCTGCCCAGG CCAAAGGGCT AATCCAGGTT GGAAAGTGGA 
     1441 ATCCAGTTCC ACTGTCTTAC GTGACAGATG CCCCTGATGC TACAGTAGCA GATATGCTTC 
     1501 AAGATGTGTA TCATGTGGTC ACATTGAAAA TTCAGTTACA CAGTTGCCCC AAACTAGAAG 
     1561 ACTTGCCTCC CGAACAATGG TCGCACACCA CAGTGAGGAA TGCTCTGAAG GACTTACTGA 
     1621 AAGATATGAA TCAGAGTTCA TTGGCCAAGG AGTGCCCCCT TTCACAGAGT ATGATTTCTT 
     1681 CCATTGTGAA CAGTACTTAC TATGCAAATG TCTCAGCAGC AAAATGTCAA GAATTTGGAA 
     1741 GGTGGTACAA ACATTTCAAG AAGACAAAAG ATATGATGGT TGAAATGGAT AGTCTTTCTG 
     1801 AGCTATCCCA GCAAGGCGCC AATCATGTCA ATTTTGGCCA GCAACCAGTT CCAGGGAACA 
     1861 CAGCCGAGCA GCCTCCATCC CCTGCGCAGC TCTCCCATGG CAGCCAGCCC TCTGTCCGGA 
     1921 CACCTCTTCC AAACCTGCAC CCTGGGCTCG TATCAACACC TATCAGTCCT CAATTGGTCA 
     1981 ACCAGCAGCT GGTGATGGCT CAGCTGCTGA ACCAGCAGTA TGCAGTGAAT AGACTTTTAG 
     2041 CCCAGCAGTC CTTAAACCAA CAATACTTGA ACCACCCTCC CCCTGTCAGT AGATCTATGA 
     2101 ATAAGCCTTT GGAGCAACAG GTTTCGACCA ACACAGAGGT GTCTTCCGAA ATCTACCAGT 
     2161 GGGTACGCGA TGAACTGAAA CGAGCAGGAA TCTCCCAGGC GGTATTTGCA CGTGTGGCTT 
     2221 TTAACAGAAC TCAGGGCTTG CTTTCAGAAA TCCTCCGAAA GGAAGAGGAC CCCAAGACTG 
     2281 CATCCCAGTC TTTGCTGGTA AACCTTCGGG CTATGCAGAA TTTCTTGCAG TTACCGGAAG 
     2341 CTGAAAGAGA CCGAATATAC CAGGACGAAA GGGAAAGGAG CTTGAATGCT GCCTCGGCCA 
     2401 TGGGTCCTGC CCCCCTCATC AGCACACCAC CCAGCCGTCC TCCCCAGGTG AAAACAGCTA 
     2461 CTATTGCCAC TGAAAGGAAT GGGAAACCAG AGAACAATAC CATGAACATT AATGCTTCCA 
     2521 TTTATGATGA GATTCAGCAG GAAATGAAGC GTGCTAAAGT GTCTCAAGCA CTGTTTGCAA 
     2581 AGGTTGCAGC AACCAAAAGC CAGGGATGGT TGTGCGAGCT GTTACGCTGG AAAGAAGATC 
     2641 CTTCTCCAGA AAACAGAACC CTGTGGGAGA ACCTCTCCAT GATCCGAAGG TTCCTCAGTC 
     2701 TTCCTCAGCC AGAACGTGAT GCCATTTATG AACAGGAGAG CAACGCGGTG CATCACCATG 
     2761 GCGACAGGCC GCCCCACATT ATCCATGTTC CAGCAGAGCA GATTCAGCAA CAGCAGCAGC 
     2821 AACAGCAACA GCAGCAGCAG CAGCAGCAGG CACCGCCGCC TCCACAGCCA CAGCAGCAGC 
     2881 CACAGACAGG CCCTCGGCTC CCCCCACGGC AACCCACGGT GGCCTCTCCA GCAGAGTCAG 
     2941 ATGAGGAAAA CCGACAGAAG ACCCGGCCAC GAACAAAAAT TTCAGTGGAA GCCTTGGGAA 
     3001 TCCTCCAGAG TTTCATACAA GACGTGGGCC TGTACCCTGA CGAAGAGGCC ATCCAGACTC 
     3061 TGTCTGCCCA GCTCGACCTT CCCAAGTACA CCATCATCAA GTTCTTTCAG AACCAGCGGT 
     3121 ACTATCTCAA GCACCACGGC AAACTGAAGG ACAATTCCGG TTTAGAGGTC GATGTGGCAG 
     3181 AATATAAAGA AGAGGAGCTG CTGAAGGATT TGGAAGAGAG TGTCCAAGAT AAAAATACTA 
     3241 ACACCCTTTT TTCAGTGAAA CTAGAAGAAG AGCTGTCAGT GGAAGGAAAC ACAGACATTA 
     3301 ATACTGATTT GAAAGACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3361 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3421 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3481 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3541 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3601 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3661 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3721 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3781 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3841 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3901 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3961 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4021 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4081 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4141 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4201 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4261 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4321 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4381 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4441 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4501 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4561 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4621 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4681 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4741 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4801 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4861 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4921 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4981 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5041 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5101 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5161 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5221 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5281 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5341 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5401 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5461 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5521 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5581 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5641 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5701 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5761 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5821 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5881 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5941 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6001 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6061 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6121 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6181 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6241 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6301 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6361 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6421 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6481 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6541 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6601 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6661 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6721 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6781 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6841 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6901 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6961 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7021 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7081 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7141 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//