LOCUS       MR227608                9444 bp    DNA     circular  SYN 
DEFINITION  Slit2 (Myc-DDK-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2)
ACCESSION   NM_178804
VERSION     NM_178804.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5601..5692 
                     /label="Myc-DDK"  
     misc_feature    5722..6307 
                     /label="PolyA signal"  
     misc_feature    5779..5798 
                     /label="Restriction Enzyme"  
     misc_feature    6583..7255 
                     /label="ColE1"  
     misc_feature    7685..8479 
                     /label="Kan/Neo"  
     misc_feature    8514..8816 
                     /label="SV40 ori"  
     misc_feature    8902..9379 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5583..5589 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5583 
                     /label="Slit2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCATT GGCTGGCAGA CACTGTCCCT ATCGCTGGGG TTAGTGTTGT 
     1081 CGATCTTGAA CAAGGTGGCG CCGCAGGCGT GCCCGGCCCA GTGCTCCTGT TCAGGCAGCA 
     1141 CGGTGGACTG TCATGGGCTG GCACTGCGCA GTGTGCCCAG GAATATCCCC CGCAACACCG 
     1201 AGAGACTGGA TTTGAATGGA AATAACATCA CGAGGATCAC GAAGATAGAT TTTGCTGGTC 
     1261 TCAGGCACCT CAGAGTTCTT CAGCTCATGG AGAACAGAAT CAGCACCATC GAGAGGGGAG 
     1321 CATTCCAGGA TCTTAAGGAG CTGGAAAGAC TGCGTTTAAA CAGAAATAAC CTTCAGTTGT 
     1381 TTCCTGAGCT GCTGTTTCTC GGGACTGCGA AGCTCTACCG GCTTGATCTC AGTGAAAATC 
     1441 AAATTCAAGC AATTCCAAGG AAGGCTTTCC GTGGGGCAGT TGACATTAAA AACCTGCAAC 
     1501 TGGATTACAA CCAGATCAGC TGCATTGAAG ATGGGGCGTT CAGAGCTCTA CGAGATCTGG 
     1561 AAGTGCTCAC TCTGAACAAT AACAATATTA CTAGACTTTC AGTGGCAAGT TTCAACCATA 
     1621 TGCCTAAACT TAGGACATTT CGACTCCACT CGAACAACTT GTACTGCGAC TGCCACCTAG 
     1681 CCTGGCTCTC AGACTGGCTT CGCCAAAGGC CACGGGTGGG CTTGTACACT CAGTGTATGG 
     1741 GCCCATCCCA CCTGAGGGGC CACAATGTAG CAGAGGTTCA AAAACGAGAG TTTGTCTGCA 
     1801 GTGGTCACCA GTCATTCATG GCTCCCTCCT GCAGTGTGCT GCACTGCCCC GCTGCTTGTA 
     1861 CCTGTAGCAA CAACATTGTA GACTGCCGAG GGAAAGGTCT CACTGAGATC CCCACAAATC 
     1921 TGCCTGAGAC CATCACAGAA ATACGTTTGG AACAGAACTC CATCAGGGTC ATCCCTCCAG 
     1981 GAGCCTTCTC ACCATACAAA AAGCTTAGAC GACTAGACCT GAGCAACAAC CAGATCTCTG 
     2041 AACTTGCACC AGATGCCTTC CAAGGACTGC GCTCTCTGAA TTCACTTGTC CTGTATGGAA 
     2101 ATAAAATCAC AGAACTCCCA AAAAGTTTAT TCGAAGGACT ATTTTCCTTG CAGCTACTAT 
     2161 TATTGAATGC CAACAAGATA AACTGCCTTC GGGTAGATGC TTTTCAGGAC CTGCACAACT 
     2221 TGAACCTTCT CTCCTTATAT GACAATAAGC TTCAGACGGT TGCCAAGGGC ACCTTCTCAG 
     2281 CCCTCAGAGC CATCCAAACT ATGCATTTGG CCCAGAATCC TTTCATTTGT GACTGCCATC 
     2341 TCAAGTGGCT AGCGGATTAT CTCCACACCA ACCCAATTGA GACCAGCGGT GCCCGTTGCA 
     2401 CCAGCCCCCG CCGCCTGGCA AACAAAAGAA TTGGACAGAT CAAAAGCAAG AAATTCCGTT 
     2461 GTTCAGGTAC AGAAGATTAT CGATCAAAAT TAAGTGGAGA CTGCTTTGCA GACTTGGCTT 
     2521 GTCCTGAGAA GTGTCGCTGT GAAGGGACCA CAGTAGACTG CTCCAATCAA AGACTCAACA 
     2581 AAATCCCTGA CCATATTCCC CAGTACACAG CAGAGCTGCG TCTCAATAAT AATGAATTCA 
     2641 CAGTGTTAGA AGCCACGGGA ATATTTAAGA AACTTCCTCA GTTACGTAAA ATCAACTTTA 
     2701 GCAACAATAA GATCACGGAT ATCGAGGAGG GTGCATTTGA AGGCGCGTCT GGTGTGAATG 
     2761 AAATTCTTCT CACCAGTAAC CGTTTGGAAA ATGTTCAGCA TAAGATGTTC AAAGGACTGG 
     2821 AGAGCCTCAA AACATTGATG CTGAGAAGTA ATCGAATAAG CTGTGTTGGG AACGACAGTT 
     2881 TCATAGGACT CGGCTCTGTG CGTCTGCTCT CTTTATATGA CAATCAAATT ACCACAGTGG 
     2941 CACCAGGAGC ATTTGATTCT CTCCATTCAT TATCCACTCT AAACCTCTTG GCCAATCCTT 
     3001 TCAACTGTAA CTGTCACCTG GCATGGCTGG GAGAATGGCT CAGAAGGAAA AGAATTGTAA 
     3061 CAGGAAATCC TCGATGCCAA AAACCCTACT TCCTGAAGGA AATCCCAATC CAGGATGTAG 
     3121 CCATTCAGGA CTTCACCTGT GATGATGGAA ATGATGACAA TAGTTGCTCT CCACTCTCCC 
     3181 GTTGTCCTTC TGAATGTACC TGCTTGGATA CAGTGGTACG ATGTAGCAAC AAGGGCTTGA 
     3241 AGGTTTTGCC TAAAGGTATT CCAAAAGATG TCACAGAGCT GTATCTGGAT GGGAACCAGT 
     3301 TTACGCTGGT CCCGAAGGAA CTCTCTAACT ACAAACATTT AACACTTATA GACTTAAGTA 
     3361 ACAACCGAAT AAGCACCCTT TCCAATCAAA GCTTCAGCAA CATGACCCAG CTTCTCACCT 
     3421 TAATCCTCAG TTACAACCGT CTGAGATGTA TCCCTCCACG AACCTTTGAT GGATTGAAGT 
     3481 CTCTTCGGTT ACTGTCTTTA CATGGAAATG ACATTTCTGT TGTGCCTGAA GGTGCCTTCA 
     3541 ATGACTTGTC AGCCTTGTCA CACTTAGCGA TTGGAGCCAA CCCTCTTTAC TGTGATTGTA 
     3601 ACATGCAGTG GTTATCCGAC TGGGTGAAGT CGGAATATAA GGAACCTGGA ATTGCACGCT 
     3661 GTGCCGGCCC TGGAGAAATG GCAGATAAAT TATTACTCAC TACTCCCTCC AAAAAATTTA 
     3721 CATGTCAAGG TCCCGTGGAT ATCACTATTC AAGCCAAGTG TAATCCCTGC TTATCAAATC 
     3781 CATGTAAAAA TGATGGCACC TGTAACAATG ACCCCGTTGA TTTTTATCGA TGTACCTGCC 
     3841 CATATGGATT CAAGGGTCAG GACTGTGATG TCCCCATTCA TGCTTGTATC AGTAATCCAT 
     3901 GTAAACATGG AGGAACTTGT CACTTAAAGG AAGGAGAGAA TGCTGGATTC TGGTGCACTT 
     3961 GTGCTGATGG GTTTGAAGGA GAAAACTGTG AAGTCAATAT TGATGATTGT GAAGATAATG 
     4021 ATTGTGAAAA TAATTCTACA TGCGTTGATG GAATTAACAA CTACACATGT CTTTGCCCAC 
     4081 CGGAATACAC AGGTGAACTG TGTGAGGAAA AGCTGGACTT CTGTGCACAA GACTTGAATC 
     4141 CCTGCCAGCA TGACTCCAAG TGCATCCTGA CTCCAAAGGG ATTCAAGTGT GACTGCACTC 
     4201 CAGGATACAT TGGTGAGCAC TGTGACATTG ACTTTGATGA CTGCCAAGAT AACAAGTGTA 
     4261 AAAACGGTGC TCACTGCACA GATGCCGTGA ACGGATACAC GTGCGTCTGT CCTGAAGGCT 
     4321 ACAGTGGCTT GTTCTGTGAG TTTTCTCCAC CCATGGTCCT CCCTCGCACC AGCCCCTGTG 
     4381 ATAATTTTGA TTGCCAGAAT GGAGCCCAGT GTATCATCAG GATAAATGAA CCAATATGCC 
     4441 AGTGTTTGCC TGGCTACCTG GGAGAGAAGT GTGAGAAATT GGTCAGTGTG AATTTTGTAA 
     4501 ACAAAGAGTC CTATCTTCAG ATTCCTTCAG CCAAGGTTCG GCCTCAGACA AACATCACAC 
     4561 TTCAGATTGC CACAGATGAA GACAGCGGCA TCCTCTTGTA TAAAGGTGAC AAAGACCACA 
     4621 TTGCCGTGGA ACTCTATAGA GGGCGAGTTC GAGCCAGCTA TGACACCGGC TCTCATCCGG 
     4681 CTTCTGCCAT TTACAGTGTG GAGACAATCA ATGATGGAAA CTTCCACATT GTGGAGCTAC 
     4741 TGACCCTGGA TTCCAGTCTT TCCCTCTCTG TGGATGGAGG AAGCCCTAAA GTCATCACCA 
     4801 ATTTGTCAAA ACAATCTACT CTGAATTTCG ACTCTCCACT CTATGTAGGA GGCATGCCTG 
     4861 GGAAAAATAA CGTGGCATCC CTGCGCCAGG CCCCTGGGCA AAATGGCACC AGCTTCCATG 
     4921 GCTGTATCCG GAACCTTTAC ATTAACAGTG AGCTGCAGGA CTTCCGGAAA ATGCCTATGC 
     4981 AAACCGGAAT TCTGCCTGGC TGTGAACCAT GCCACAAGAA AGTATGTGCC CATGGCATGT 
     5041 GCCAGCCCAG CAGCCAATCA GGCTTCACCT GTGAATGTGA GGAAGGGTGG ATGGGGCCCC 
     5101 TCTGTGACCA GAGAACCAAT GATCCCTGCC TCGGAAACAA ATGTGTGCAT GGGACCTGCC 
     5161 TGCCCATCAA TGCCTTCTCC TATAGTTGCA AGTGCCTGGA GGGCCATGGC GGTGTCCTCT 
     5221 GTGATGAAGA AGAAGATCTC TTTAACCCCT GCCAGATGAT CAAGTGCAAG CATGGGAAGT 
     5281 GCAGGCTTTC TGGAGTGGGC CAGCCCTATT GTGAATGCAA CAGTGGATTC ACCGGGGACA 
     5341 GCTGTGATAG AGAAATTTCT TGTCGAGGGG AACGGATAAG GGACTATTAC CAGAAGCAGC 
     5401 AGGGTTACGC TGCCTGTCAA ACAACTAAGA AAGTATCTCG CTTGGAATGC AGAGGCGGGT 
     5461 GCGCTGGAGG CCAGTGCTGT GGACCTCTGA GAAGCAAGAG GCGGAAATAC TCTTTCGAAT 
     5521 GCACAGATGG CTCCTCATTT GTGGACGAGG TTGAGAAAGT GGTGAAGTGC GGCTGCGCGA 
     5581 GATGTGCCTC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5641 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5701 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5761 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5821 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5881 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5941 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     6001 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     6061 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     6121 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     6181 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6241 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6301 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6361 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6421 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6481 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6541 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6601 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6661 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6721 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6781 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6841 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6901 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6961 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     7021 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     7081 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     7141 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     7201 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7261 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7321 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7381 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7441 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7501 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7561 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7621 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7681 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7741 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7801 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7861 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7921 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7981 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     8041 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     8101 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     8161 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     8221 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8281 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8341 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8401 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8461 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8521 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8581 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8641 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8701 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8761 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8821 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8881 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8941 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     9001 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     9061 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     9121 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     9181 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9241 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9301 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9361 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9421 TAACAAAAAT TTAACGCGAA TTTT
//