LOCUS       MR227576                6409 bp    DNA     circular  SYN 
DEFINITION  Smad6 (Myc-DDK-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6)
ACCESSION   NM_008542
VERSION     NM_008542.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2569..2660 
                     /label="Myc-DDK"  
     misc_feature    2690..3275 
                     /label="PolyA signal"  
     misc_feature    2747..2766 
                     /label="Restriction Enzyme"  
     misc_feature    3551..4223 
                     /label="ColE1"  
     misc_feature    4653..5447 
                     /label="Kan/Neo"  
     misc_feature    5482..5784 
                     /label="SV40 ori"  
     misc_feature    5870..6347 
                     /label="f1 ori"  
     misc_feature    1044..1050 
                     /label="Restriction Enzyme"  
     misc_feature    2544..2551 
                     /label="Restriction Enzyme"  
     misc_feature    1044..2544 
                     /label="Smad6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCAGAT CTCAAGCTTA AGCTTATGTT CAGGTCTAAA CGTTCGGGGC 
     1081 TGGTGCGGCG ACTTTGGCGA AGTCGTGTGG TCCCTGATCG GGAGGAAGGC AGCGGCGGCG 
     1141 GCGGTGGTGT CGACGAGGAT GGGAGCCTGG GCAGCCGAGC TGAGCCTGCC CCGCGGGCAC 
     1201 GAGAGGGCGG AGGCTGCAGC CGCTCCGAAG TCCGCTCGGT AGCCCCGCGG CGGCCCCGGG 
     1261 ACGCGGTGGG ACCGCGAGGC GCCGCGATCG CGGGCAGGCG CCGGCGCACA GGGGGCCTCC 
     1321 CGAGGCCCGT GTCGGAGTCG GGGGCCGGGG CTGGGGGCTC CCCGCTGGAT GTGGCGGAGC 
     1381 CTGGAGGCCC AGGCTGGCTG CCTGAGAGTG ACTGCGAGAC GGTGACCTGC TGTCTCTTCT 
     1441 CCGAACGGGA CGCAGCAGGC GCGCCCCGGG ACTCTGGCGA TCCCCAAGCC AGACAGTCCC 
     1501 CGGAGCCGGA GGAGGGCGGC GGGCCTCGGA GTCGCGAAGC CCGCTCGCGA CTGCTGCTTC 
     1561 TGGAGCAGGA GCTCAAGACG GTCACGTACT CGCTGCTCAA GAGGCTCAAG GAGCGTTCGC 
     1621 TGGACACGCT GTTGGAGGCT GTGGAGTCCC GAGGCGGCGT ACCGGGCGGC TGCGTGCTGG 
     1681 TGCCGCGCGC CGACCTCCGC TTGGGCGGCC AGCCCGCGCC ACCGCAGCTG CTGCTCGGCC 
     1741 GCCTCTTCCG CTGGCCAGAC CTGCAGCACG CAGTGGAGCT GAAACCCCTG TGCGGCTGCC 
     1801 ACAGCTTTAC CGCCGCCGCC GACGGGCCCA CGGTGTGTTG CAACCCCTAC CACTTCAGCC 
     1861 GGCTCTGCGG GCCAGAATCA CCGCCGCCCC CCTATTCTCG GCTGTCTCCT CCTGACCAGT 
     1921 ACAAGCCACT GGATCTGTCC GATTCTACAT TGTCTTACAC TGAAACCGAG GCCACCAACT 
     1981 CCCTCATCAC TGCTCCGGGT GAATTCTCAG ATGCCAGCAT GTCTCCGGAT GCCACCAAGC 
     2041 CGAGCCACTG GTGCAGCGTG GCGTACTGGG AGCACCGGAC ACGCGTGGGC CGCCTCTATG 
     2101 CGGTGTACGA CCAGGCTGTC AGCATTTTCT ACGACCTACC TCAGGGCAGC GGCTTCTGCC 
     2161 TGGGCCAGCT CAACCTGGAG CAGCGCAGTG AGTCGGTGCG GCGCACGCGC AGCAAGATCG 
     2221 GTTTTGGCAT ACTGCTCAGC AAGGAGCCAG ACGGCGTGTG GGCCTACAAC CGGGGCGAGC 
     2281 ACCCCATCTT CGTCAACTCC CCGACGCTGG ATGCGCCCGG AGGCCGCGCC CTGGTCGTGC 
     2341 GCAAGGTGCC ACCGGGTTAC TCCATCAAGG TGTTCGACTT TGAGCGCTCA GGGCTGCTGC 
     2401 AGCACGCAGA CGCCGCTCAC GGCCCCTACG ACCCGCACAG TGTGCGCATC AGCTTCGCCA 
     2461 AGGGCTGGGG ACCCTGCTAC TCGCGACAGT TCATCACCTC CTGCCCCTGT TGGCTGGAGA 
     2521 TCCTACTCAA CAACCACAGA AGCGGACCGC GGACCGACGC GTACGCGGCC GCTCGAGCAG 
     2581 AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC 
     2641 GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA 
     2701 TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC 
     2761 CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA 
     2821 TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG 
     2881 GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC 
     2941 AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT 
     3001 CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC 
     3061 CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC 
     3121 CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA 
     3181 ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC 
     3241 GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA 
     3301 GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     3361 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     3421 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     3481 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     3541 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     3601 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     3661 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     3721 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     3781 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     3841 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     3901 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     3961 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 
     4021 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     4081 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     4141 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     4201 ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC 
     4261 CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC 
     4321 AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT 
     4381 GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG 
     4441 TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA 
     4501 ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC 
     4561 CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG 
     4621 GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG 
     4681 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG 
     4741 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG 
     4801 ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC 
     4861 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG 
     4921 CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC 
     4981 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG 
     5041 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC 
     5101 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC 
     5161 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC 
     5221 TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC 
     5281 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG 
     5341 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG 
     5401 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA 
     5461 CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC 
     5521 CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA 
     5581 AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG 
     5641 GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC 
     5701 TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC 
     5761 TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT 
     5821 TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA 
     5881 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 
     5941 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT 
     6001 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 
     6061 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 
     6121 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 
     6181 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 
     6241 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 
     6301 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 
     6361 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//