LOCUS       MR227524                8709 bp    DNA     circular  SYN 
DEFINITION  Abcb1a (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a). Note: ORF is codon optimized
ACCESSION   NM_011076
VERSION     NM_011076.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4866..4957 
                     /label="Myc-DDK"  
     misc_feature    4987..5572 
                     /label="PolyA signal"  
     misc_feature    5044..5063 
                     /label="Restriction Enzyme"  
     misc_feature    5848..6520 
                     /label="ColE1"  
     misc_feature    6950..7744 
                     /label="Kan/Neo"  
     misc_feature    7779..8081 
                     /label="SV40 ori"  
     misc_feature    8167..8644 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4848..4854 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4848 
                     /label="Abcb1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTTGGAG GAGGACCTCA AGGGCCGAGC GGACAAAAAT TTTAGTAAGA 
     1081 TGGGCAAGAA ATCCAAGAAA GAGAAGAAGG AAAAAAAGCC AGCCGTGTCT GTGCTCACAA 
     1141 TGTTCCGATA CGCAGGGTGG CTTGATCGGC TCTACATGCT CGTCGGAACG TTGGCTGCCA 
     1201 TCATCCACGG AGTAGCGCTG CCTTTGATGA TGTTGATTTT CGGTGACATG ACCGACTCAT 
     1261 TCGCATCCGT TGGGAACGTG TCTAAGAACA GCACAAACAT GAGTGAGGCT GACAAAAGGG 
     1321 CAATGTTCGC AAAGCTTGAA GAGGAGATGA CGACCTACGC ATACTACTAC ACAGGTATCG 
     1381 GCGCAGGGGT CCTCATCGTG GCCTACATCC AGGTGAGTTT CTGGTGTCTG GCCGCTGGCA 
     1441 GACAAATTCA CAAGATCAGA CAAAAATTCT TTCACGCTAT TATGAATCAG GAGATTGGAT 
     1501 GGTTTGATGT CCACGATGTC GGAGAGCTGA ACACCCGGCT GACCGACGAT GTTAGCAAGA 
     1561 TTAATGAAGG TATCGGAGAC AAGATCGGCA TGTTTTTCCA GGCTATGGCG ACCTTCTTCG 
     1621 GCGGTTTTAT TATAGGATTC ACCCGGGGTT GGAAACTCAC TCTGGTGATA CTTGCTATCA 
     1681 GTCCAGTGCT CGGACTGTCT GCTGGCATAT GGGCCAAAAT TCTCAGCTCA TTCACTGACA 
     1741 AAGAACTGCA CGCATATGCC AAAGCAGGTG CAGTGGCAGA GGAAGTACTC GCTGCCATTC 
     1801 GCACCGTCAT AGCCTTTGGG GGTCAGAAGA AGGAGCTGGA AAGGTACAAC AATAACCTGG 
     1861 AAGAGGCTAA GAGGCTCGGA ATAAAAAAGG CCATTACCGC CAACATCAGC ATGGGAGCGG 
     1921 CATTCCTTCT CATCTACGCC TCTTACGCCC TGGCCTTCTG GTACGGAACT AGCCTGGTCA 
     1981 TTTCCAAAGA ATACAGCATC GGGCAGGTGC TTACCGTGTT CTTCTCCGTC CTCATCGGCG 
     2041 CTTTCTCCGT CGGTCAGGCC AGCCCCAATA TCGAGGCATT CGCTAATGCC AGAGGAGCCG 
     2101 CATATGAAGT ATTTAAGATC ATTGACAACA AGCCATCCAT CGACTCTTTT TCTAAATCTG 
     2161 GACACAAGCC AGATAACATC CAAGGGAACC TGGAATTTAA AAATATTCAC TTCAGTTATC 
     2221 CCTCCCGGAA GGAGGTCCAG ATCCTTAAAG GACTCAATCT TAAAGTCAAA TCCGGCCAGA 
     2281 CTGTAGCACT CGTCGGTAAT TCTGGGTGTG GGAAGAGTAC CACAGTGCAG TTGATGCAGC 
     2341 GCCTCTATGA CCCCTTGGAC GGGATGGTCA GCATAGACGG GCAAGATATT AGGACAATCA 
     2401 ACGTTCGATA TCTGCGAGAA ATTATCGGTG TCGTGAGCCA AGAACCAGTC CTCTTCGCCA 
     2461 CTACTATTGC AGAGAACATC AGATATGGCC GAGAGGATGT CACAATGGAC GAGATTGAGA 
     2521 AGGCTGTCAA AGAGGCTAAT GCTTACGATT TCATCATGAA GCTGCCTCAC CAGTTTGACA 
     2581 CACTCGTTGG TGAGAGGGGC GCTCAATTGT CCGGCGGACA GAAGCAAAGG ATCGCAATTG 
     2641 CGCGGGCGCT CGTGCGCAAC CCAAAAATAT TGCTGTTGGA CGAAGCCACC TCTGCACTGG 
     2701 ACACCGAGTC CGAGGCCGTG GTGCAGGCTG CCCTTGATAA GGCTCGGGAG GGCCGAACCA 
     2761 CTATTGTCAT TGCACACCGC CTCTCCACAG TGAGAAACGC CGACGTCATC GCAGGGTTCG 
     2821 ATGGCGGCGT CATTGTGGAA CAGGGCAACC ACGATGAACT CATGCGCGAG AAGGGAATAT 
     2881 ATTTTAAACT GGTGATGACC CAGACCGCGG GCAATGAGAT CGAACTGGGA AACGAGGCCT 
     2941 GTAAGAGCAA GGATGAAATT GACAACCTGG ACATGTCTAG CAAAGACTCC GGATCTTCTT 
     3001 TGATTAGGAG GCGCTCCACG CGGAAGTCCA TTTGTGGACC ACATGATCAA GACCGCAAGC 
     3061 TCTCCACCAA GGAGGCACTG GACGAGGACG TTCCTCCTGC CTCATTCTGG CGGATTCTGA 
     3121 AATTGAACAG CACAGAATGG CCATACTTTG TTGTGGGAAT CTTTTGCGCT ATTATTAACG 
     3181 GGGGGCTCCA ACCAGCATTC TCCGTCATTT TCTCCAAGGT GGTGGGCGTC TTCACTAATG 
     3241 GTGGACCACC AGAAACTCAA AGACAGAATA GTAACTTGTT TTCACTTCTC TTCCTTATAC 
     3301 TCGGCATTAT TAGCTTTATT ACGTTTTTCC TGCAGGGGTT TACATTTGGC AAGGCTGGAG 
     3361 AGATTCTGAC AAAGCGCCTG AGATACATGG TCTTTAAGTC AATGCTTAGG CAGGACGTCA 
     3421 GCTGGTTCGA CGATCCCAAA AATACTACCG GAGCTCTTAC CACTCGCCTT GCCAATGACG 
     3481 CCGCTCAGGT GAAGGGAGCT ACAGGCAGTC GACTTGCTGT AATCTTCCAG AACATCGCTA 
     3541 ACTTGGGGAC TGGAATCATC ATATCCTTGA TATATGGCTG GCAACTTACA TTGCTTCTTC 
     3601 TCGCCATTGT TCCTATTATT GCAATCGCTG GAGTCGTTGA GATGAAAATG CTGTCAGGGC 
     3661 AGGCCCTGAA AGACAAAAAG GAATTGGAGG GTAGTGGCAA AATTGCCACA GAAGCAATTG 
     3721 AAAACTTCAG AACTGTTGTC TCTTTGACGA GAGAACAAAA ATTCGAAACA ATGTATGCCC 
     3781 AGTCTTTGCA GATCCCATAC AGGAACGCAA TGAAAAAGGC CCACGTGTTC GGTATCACTT 
     3841 TTTCTTTTAC ACAGGCAATG ATGTATTTCT CTTACGCTGC CTGTTTTAGA TTCGGCGCGT 
     3901 ATCTTGTGAC GCAGCAGCTG ATGACTTTCG AGAATGTCCT TCTTGTATTT AGTGCCATCG 
     3961 TGTTTGGGGC AATGGCGGTC GGGCAGGTAA GCTCCTTCGC CCCTGACTAT GCTAAGGCAA 
     4021 CGGTGAGCGC TAGCCATATC ATCAGGATCA TAGAGAAGAC TCCCGAGATA GATTCCTATT 
     4081 CTACCCAGGG GCTGAAGCCT AACATGCTGG AAGGCAACGT CCAATTTTCT GGGGTTGTGT 
     4141 TCAATTATCC GACTCGACCC TCCATTCCCG TACTGCAAGG CCTCTCTCTC GAGGTCAAAA 
     4201 AGGGCCAAAC TCTCGCCTTG GTTGGCTCAT CTGGATGTGG CAAGAGCACT GTTGTGCAAT 
     4261 TGCTGGAGCG ATTTTATGAT CCAATGGCTG GCTCAGTGTT TCTGGACGGC AAGGAGATCA 
     4321 AGCAGCTCAA CGTGCAGTGG CTGCGCGCCC AGCTTGGGAT AGTCAGTCAG GAACCAATCC 
     4381 TGTTTGACTG CTCAATAGCT GAGAACATTG CCTACGGGGA CAATAGCCGG GTCGTTTCAT 
     4441 ACGAGGAAAT TGTTAGGGCT GCGAAAGAGG CTAACATTCA TCAATTTATC GACTCCCTGC 
     4501 CTGACAAGTA TAATACAAGG GTTGGAGATA AAGGTACACA GCTGTCAGGT GGTCAGAAGC 
     4561 AGCGGATCGC TATTGCTCGC GCTCTGGTTC GACAGCCACA CATCTTGCTT TTGGATGAAG 
     4621 CCACCTCAGC ATTGGATACC GAGTCCGAGA AAGTCGTGCA GGAGGCTCTG GATAAGGCTA 
     4681 GAGAAGGAAG GACATGTATC GTAATCGCAC ATCGGCTTTC CACTATCCAG AACGCAGACC 
     4741 TGATAGTGGT CATCCAGAAC GGAAAAGTCA AGGAACATGG GACCCACCAG CAGCTTCTTG 
     4801 CTCAGAAGGG AATTTACTTC TCCATGGTGT CCGTCCAGGC AGGCGCTAAG CGGAGCACGC 
     4861 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4921 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4981 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5041 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5101 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5161 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5221 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5281 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5341 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5401 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5461 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5521 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5581 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5641 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5701 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5761 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5821 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5881 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5941 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6001 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6061 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6121 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6181 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6241 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6301 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6361 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6421 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6481 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6541 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6601 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6661 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6721 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6781 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6841 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6901 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6961 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7021 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7081 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7141 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7201 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7261 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7321 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7381 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7441 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7501 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7561 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7621 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7681 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7741 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7801 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7861 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7921 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7981 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8161 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8221 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8281 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8341 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8401 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8461 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8521 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8581 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8641 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8701 GCGAATTTT
//