LOCUS       MR227454                8301 bp    DNA     circular  SYN 
DEFINITION  Trim33 (Myc-DDK-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 1
ACCESSION   NM_053170
VERSION     NM_053170.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4458..4549 
                     /label="Myc-DDK"  
     misc_feature    4579..5164 
                     /label="PolyA signal"  
     misc_feature    4636..4655 
                     /label="Restriction Enzyme"  
     misc_feature    5440..6112 
                     /label="ColE1"  
     misc_feature    6542..7336 
                     /label="Kan/Neo"  
     misc_feature    7371..7673 
                     /label="SV40 ori"  
     misc_feature    7759..8236 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4440..4446 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4440 
                     /label="Trim33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAAAC AAAGGCGGCG GTGAGGCAGA GAGTGGCGGC GGGGGCAGCG 
     1081 GTAGCGCGCC GGTAACCGCC GGGGCCGCCG GGCCCACGGC GCAGGAGGCG GAGCCGCCTC 
     1141 TCGCCGCGGT GCTGGTGGAG GAGGAGGAGG AGGAAGGCGG CAGGGCTGGC GCGGAGGGCG 
     1201 GTGCGGCTGG GCCCGACGAC GGGGGGGTGG CCGCGGCCTC CTCAAGCTCG GCCCCGGCCG 
     1261 CCTCGGTCCC CGCGGCCTCA GTGGGCTCCG CGGTCCCCGG AGGCGCGGCA TCGACGCCGG 
     1321 CTCCAGCTGC TGCCCCAGCC CCGGCCCCGG CCCCGGCCCC GGCCCCGGCC CCCGCTCCCG 
     1381 CCCCGGCTCC TGGTTCCTCG TCGGGGCCAC CTCTCGGACC ACCAGCCTCG CTTCTGGACA 
     1441 CCTGCGCGGT GTGTCAGCAG AGCTTGCAGA GCCGGCGCGA GGCGGAGCCC AAGCTGCTCC 
     1501 CCTGCCTGCA CTCCTTCTGC CTGCGCTGTC TGCCGGAGCC GGAGCGCCAG CTCAGCGTGC 
     1561 CCATCCCTGG GGGCAGCAAC GGTGACGTCC AGCAAGTTGG TGTAATACGG TGTCCAGTAT 
     1621 GTCGTCAAGA ATGTAGACAG ATAGATCTTG TGGATAATTA TTTTGTGAAA GACACATCTG 
     1681 AAGCTCCTAG CAGCTCTGAT GAGAAATCAG AACAGGTATG TACTAGTTGT GAAGATAATG 
     1741 CAAGTGCAGT TGGTTTTTGT GTAGAATGTG GAGAGTGGCT GTGTAAGACA TGCATTGAAG 
     1801 CACATCAAAG AGTAAAATTC ACTAAAGACC ACCTGATCAG GAAAAAAGAA GATGTCTCAG 
     1861 AGTCTGTTGG AACATCTGGT CAGCGCCCAG TGTTTTGTCC TGTACATAAA CAAGAGCAGT 
     1921 TGAAACTATT CTGTGAAACG TGTGATAGAT TGACGTGTAG AGACTGTCAA CTTTTGGAGC 
     1981 ACAAAGAACA TAGGTATCAG TTTTTAGAAG AGGCTTTTCA AAATCAGAAA GGTGCAATTG 
     2041 AAAATCTGTT GGCTAAGCTT CTTGAAAAGA AGAATTATGT TCATTTTGCA GCTACACAAG 
     2101 TGCAGAATAG GATTAAAGAA GTAAATGAGA CTAACAAACG TGTAGAACAG GAAATTAAAG 
     2161 TGGCCATCTT CACCCTTATC AATGAAATTA ATAAGAAAGG AAAATCTCTC TTGCAACAGC 
     2221 TAGAGAATGT TACAAAAGAA AGACAGATGA AGTTACTACA GCAGCAGAAT GATATCACAG 
     2281 GCCTCTCCCG GCAGGTGAAG CATGTTATGA ACTTTACAAA CTGGGCCATT GCAAGTGGCA 
     2341 GCAGCACGGC CCTACTTTAT AGCAAGCGTC TGATTACTTT TCAGTTACGT CATATTTTGA 
     2401 AAGCCCGATG TGATCCTGTT CCTGCTGCCA ATGGAGCAAT TCGTTTCCAT TGTGATCCCA 
     2461 CCTTCTGGGC CAAAAATGTA GTGAATTTAG GTAATCTAGT AATAGAGAGT AAGCCAGCTC 
     2521 CTGGTTATAC TCCTAATGTT GTAGTTGGGC AAGTTCCTCC AGGGACAAAC CACATTAGTA 
     2581 AAACCCCTGG ACAGATTAAT TTAGCACAGC TTCGACTCCA GCACATGCAG CAACAAGTGT 
     2641 ATGCACAGAA ACATCAGCAG TTACAACAGA TGAGACTGCA GCAACCACCA GCTCCTATAC 
     2701 CAACAACAAC AGCAACAACC CAGCAGCATC CTAGGCAAGC AGCCCCACAG ATGTTACAAC 
     2761 AACAGCCTCC AAGATTGATC AGTGTGCAAA CAATGCAAAG AGGCAACATG AACTGTGGAG 
     2821 CATTTCAAGC CCATCAAATG AGACTGGCTC AGAATGCTGC CAGAATACCA GGGATCCCCA 
     2881 GGCACAGTGC CCCTCAGTAT TCCATGATGC AGCCACATCT CCAAAGACAA CATTCAAATC 
     2941 CAGGACATGC TGGACCGTTT CCTGTTGTAT CAGCACACAA CCCAATCAAC CCAACAAGTC 
     3001 CCACCACAGC AACTATGGCA AATGCAAATC GAGGTCCCAC CAGCCCGTCT GTTACAGCAA 
     3061 TAGAATTAAT ACCCTCAGTC ACCAATCCAG AAAACCTTCC ATCGTTGCCA GATATCCCAC 
     3121 CTATACAGTT GGAAGATGCT GGCTCAAGTA GTTTAGATAA TCTACTGAGT AGATATATCT 
     3181 CAGGCAGTCA TCTACCCCCT CAGCCTACAA GTACCATGAA TCCTTCCCCA GGACCCTCTG 
     3241 CGTTATCACC TGGATCTTCA GGCTTATCCA ATTCTCACAC ACCTGTGAGA CCCCCTAGCA 
     3301 CGTCTAGTAC TGGCAGTCGA GGCAGCTGCG GATCATCAGG AAGAACTGCT GAGAAAAGTG 
     3361 CTCATAGTTT CAAGTCTGAT CAGGTGAAGG TCAAGCAGGA ACCTGGGACT GAAGAAGAGA 
     3421 TATGCAGCTT TTCAGGAGCT GTGAAACAAG AGAAGACAGA GGATGGCAGG AGGAGTGCCT 
     3481 GTATGTTGAG CAGTCCTGAG AGTAGTTTGA CACCACCTCT TTCAACTAAC CTTCACCTAG 
     3541 AGAGTGAGCT GGATACATTA ACAGGCCTGG AGAACCACGT GAAGACTGAA CCTACAGATA 
     3601 TCAGTGAAAG CTGCAAACAG TCAGGACTCA GTAACCTAGT CAATGGAAAG TCTCCAATTC 
     3661 GAAACCTCAT GCACAGGTCG GCAAGGATTG GAGGGGATGG CAATAGCAAA GATGATGACC 
     3721 CAAATGAAGA CTGGTGTGCC GTCTGCCAGA ACGGAGGGGA TCTTTTGTGT TGTGAGAAAT 
     3781 GTCCGAAGGT CTTTCATCTT ACCTGTCATG TTCCAACACT TCTCAGCTTT CCAAGTGGAG 
     3841 ACTGGATATG CACATTTTGC AGAGATATTG GGAAGCCAGA AGTTGAATAT GATTGTGATA 
     3901 ATATGCAGCA TAGTAAGAAG GGGAAAACTG CACAAGGCTT AAGCCCTGTG GACCAAAGGA 
     3961 AATGTGAACG TCTTCTGCTT TACCTCTATT GTCACGAATT AAGTATTGAA TTCCAGGAGC 
     4021 CTGTTCCTGT TTCGATACCA AACTACTATA AAATTATAAA GAAACCGATG GATTTATCAA 
     4081 CGGTGAAGAA GAAGCTTCAG AAAAAACATT CCCAGCATTA CCAAATCCCA GATGACTTTG 
     4141 TGGCTGATGT CCGTTTGATC TTCAAGAACT GTGAAAGGTT TAATGAAATG ATGAAAGTTG 
     4201 TTCAAGTTTA TGCAGACACA CAAGAGATTA ATTTGAAGGG TGATTCAGAA GTAGCTAAGG 
     4261 CAGGGAAAGC AGTTGCATTG TACTTTGAAG ATAAACTCTC AGAGATCTAC TCAGACAGGA 
     4321 CCTTCACTCC TTTGCCAGAG TTTGAGCAGG ACGAAGATGA TGGTGAAGTA ACAGAAGACT 
     4381 CTGACGAAGA TTTTATACAG CCTCGTAGAA AACGCCTAAA GTCAGACGAG AGACCAGTAC 
     4441 ATATAAAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4501 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4561 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4621 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4681 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4741 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4801 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4861 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4921 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4981 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5041 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5101 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5161 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5221 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5281 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5341 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5401 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5461 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5521 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5581 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5641 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5701 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5761 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5821 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5881 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5941 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6001 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6061 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6121 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6181 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6241 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6301 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6361 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6421 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6481 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6541 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6601 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6661 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6721 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6781 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6841 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6901 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6961 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7021 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7081 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7141 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7201 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7261 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7321 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7381 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7441 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7501 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7561 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7621 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7741 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7801 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7861 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7921 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7981 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8041 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8101 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8161 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8221 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8281 CAAAAATTTA ACGCGAATTT T
//