LOCUS       MR227419                9168 bp    DNA     circular  SYN 
DEFINITION  Nos1 (Myc-DDK-tagged) - Mouse nitric oxide synthase 1, neuronal (Nos1)
ACCESSION   NM_008712
VERSION     NM_008712.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5325..5416 
                     /label="Myc-DDK"  
     misc_feature    5446..6031 
                     /label="PolyA signal"  
     misc_feature    5503..5522 
                     /label="Restriction Enzyme"  
     misc_feature    6307..6979 
                     /label="ColE1"  
     misc_feature    7409..8203 
                     /label="Kan/Neo"  
     misc_feature    8238..8540 
                     /label="SV40 ori"  
     misc_feature    8626..9103 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5307..5313 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5307 
                     /label="Nos1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGAGCAC ACGTTTGGGG TCCAGCAGAT CCAACCCAAC GTCATTTCTG 
     1081 TCCGTCTCTT CAAACGCAAA GTGGGAGGTC TGGGCTTCCT GGTGAAGGAA CGGGTCAGCA 
     1141 AGCCCCCGGT GATCATCTCA GATCTGATCC GAGGAGGTGC CGCGGAGCAG AGCGGCCTTA 
     1201 TCCAAGCCGG CGACATCATT CTCGCCGTCA ATGATCGGCC CCTGGTAGAC CTCAGCTATG 
     1261 ACAGCGCTCT GGAAGTTCTC AGGGGCATTG CCTCTGAGAC CCATGTGGTC CTCATTCTGA 
     1321 GAGGCCCTGA GGGCTTTACT ACACACCTGG AGACCACCTT CACAGGGGAT GGAACCCCCA 
     1381 AGACCATCCG TGTGACCCAG CCCCTGGGGA CACCCACCAA AGCTGTCGAT CTGTCTCGCC 
     1441 AGCCATCAGC CAGCAAAGAC CAGCCATTAG CAGTAGACAG GGTCCCAGGT CCCAGTAACG 
     1501 GACCTCAGCA TGCCCAAGGC CGTGGACAGG GAGCCGGCTC AGTCTCCCAG GCTAATGGTG 
     1561 TGGCCATTGA CCCCACAATG AAAAATACCA AGGCCAACCT CCAGGACAGC GGAGAACAGG 
     1621 ATGAACTGCT CAAAGAGATA GAACCTGTGT TGAGCATCCT CACCGGTGGG GGAAAAGCAG 
     1681 TCAACAGAGG GGGACCAGCC AAAGCAGAGA TGAAAGACAC AGGAATCCAG GTGGACAGAG 
     1741 ACCTCGACGG CAAACTGCAC AAAGCTCCGC CCCTGGGCGG GGAGAATGAT CGAGTCTTCA 
     1801 ATGATCTGTG GGGGAAGGGC AACGTCCCTG TGGTCCTCAA CAACCCGTAT TCAGAGAACG 
     1861 AACAGTCTCC CGCCTCGGGC AAACAGTCTC CTACCAAGAA TGGCAGTCCT TCCAGGTGCC 
     1921 CTCGCTTCCT TAAGGTCAAG AACTGGGAGA CAGATGTGGT CCTCACTGAC ACCCTGCACC 
     1981 TGAAGAGCAC GCTGGAAACG GGGTGCACAG AGCAGATCTG CATGGGCTCC ATCATGCTGC 
     2041 CATCCCATCA CATACGGAAG TCAGAAGATG TCCGCACCAA GGACCAGCTC TTCCCTCTAG 
     2101 CCAAAGAATT TCTCGACCAA TACTACTCCT CCATTAAGAG GTTTGGCTCC AAGGCCCACA 
     2161 TGGACAGGCT GGAGGAGGTG AACAAGGAGA TCGAGAGCAC CAGCACCTAC CAGCTCAAGG 
     2221 ACACGGAGCT CATCTATGGC GCCAAGCATG CCTGGCGGAA CGCCTCTCGC TGTGTGGGCA 
     2281 GGATCCAGTG GTCCAAGCTG CAGGTGTTCG ATGCCCGAGA CTGCACCACA GCCCATGGGA 
     2341 TGTTCAACTA CATCTGTAAC CACGTCAAGT ACGCCACCAA CAAAGGGAAC CTCAGGTCGG 
     2401 CCATCACTAT ATTCCCTCAA AGGACTGATG GCAAGCATGA CTTCCGAGTG TGGAACTCGC 
     2461 AGCTCATCCG CTATGCCGGC TACAAGCAGC CAGATGGCTC TACCTTGGGC GATCCAGCTA 
     2521 ATGTGGAGTT CACAGAGATC TGTATACAGC AGGGCTGGAA ACCCCCAAGA GGCCGGTTCG 
     2581 ATGTGCTGCC TCTCCTGCTC CAGGCCAATG GCAATGACCC TGAACTCTTT CAGATCCCCC 
     2641 CAGAGCTGGT GCTGGAAGTA CCCATCAGGC ACCCCAAGTT CGACTGGTTT AAGGACCTGG 
     2701 GGCTCAAATG GTATGGCCTC CCCGCTGTGT CCAACATGCT GCTGGAGATT GGGGGCCTGG 
     2761 AGTTCAGCGC CTGTCCCTTT AGTGGCTGGT ACATGGGCAC GGAGATTGGC GTTCGTGATT 
     2821 ACTGTGACAA CTCTCGATAC AACATCCTGG AGGAAGTAGC CAAGAAAATG GATTTGGACA 
     2881 TGAGGAAGAC ATCCTCCCTC TGGAAGGACC AAGCCCTGGT GGAGATTAAC ATTGCTGTCC 
     2941 TATACAGCTT CCAGAGTGAC AAGGTGACCA TTGTGGACCA CCACTCTGCC ACCGAGTCCT 
     3001 TCATCAAACA CATGGAGAAT GAGTACCGCT GCAGAGGGGG CTGTCCCGCC GACTGGGTGT 
     3061 GGATTGTGCC CCCCATGTCG GGCAGCATCA CCCCTGTCTT CCACCAGGAG ATGCTCAACT 
     3121 ACCGGCTCAC CCCGTCCTTT GAGTACCAGC CTGATCCATG GAACACCCAC GTGTGGAAGG 
     3181 GCACCAATGG GACCCCCACG AAGCGGCGAG CCATTGGCTT CAAGAAATTG GCAGAGGCCG 
     3241 TCAAGTTCTC AGCCAAGCTG ATGGGACAGG CCATGGCCAA GAGAGTCAAA GCGACCATTC 
     3301 TCTACGCCAC CGAGACAGGC AAATCCCAAG CCTATGCCAA GACCCTGTGC GAGATCTTCA 
     3361 AGCATGCCTT CGATGCCAAG GCTATGTCCA TGGAGGAATA TGACATCGTG CACCTGGAGC 
     3421 ACGAAGCCCT GGTCTTGGTG GTCACCAGCA CCTTTGGCAA TGGAGACCCC CCAGAGAATG 
     3481 GGGAGAAATT CGGCTGTGCT TTGATGGAGA TGAGGCACCC CAACTCTGTG CAAGAGGAGA 
     3541 GGAAGAGCTA CAAGGTCCGA TTCAACAGCG TCTCCTCCTA TTCTGACTCC CGCAAGTCAT 
     3601 CAGGCGATGG ACCAGACCTC AGAGACAACT TTGAAAGTAC TGGACCGCTG GCCAATGTGA 
     3661 GGTTCTCAGT CTTCGGCCTG GGCTCTCGGG CATACCCTCA CTTCTGTGCC TTTGGGCACG 
     3721 CAGTGGACAC TCTCCTGGAG GAGCTGGGCG GGGAGAGGAT TCTGAAGATG AGGGAGGGAG 
     3781 ATGAGCTCTG CGGACAGGAA GAGGCTTTCA GGACCTGGGC TAAGAAAGTC TTCAAGGCAG 
     3841 CCTGTGATGT GTTCTGTGTG GGGGATGACG TCAACATTGA GAAGGCGAAC AATTCCCTCA 
     3901 TTAGCAATGA CCGCAGCTGG AAGAGGAATA AATTCCGCCT CACTTATGTG GCAGAAGCTC 
     3961 CAGAGCTGAC CCAAGGTCTT TCCAATGTTC ACAAAAAGCG AGTCTCAGCC GCCCGACTCC 
     4021 TCAGCCGCCA AAACCTGCAA AGTCCTAAAT CCAGCCGATC GACCATCTTC GTGCGTCTCC 
     4081 ACACCAACGG GAATCAGGAG TTGCAGTACC AGCCAGGGGA CCACCTGGGT GTCTTCCCTG 
     4141 GCAACCATGA GGACCTCGTG AATGCACTCA TTGAACGGCT GGAGGACGCG CCGCCTGCCA 
     4201 ACCACGTGGT GAAGGTGGAA ATGCTGGAGG AAAGGAACAC GGCTCTGGGT GTCATCAGTA 
     4261 ATTGGAAGGA TGAATCTCGC CTCCCACCCT GCACCATCTT CCAGGCCTTC AAGTACTACC 
     4321 TGGACATCAC CACGCCGCCC ACGCCCCTGC AGCTGCAGCA GTTCGCCTCC CTGGCCACCA 
     4381 ATGAGAAAGA GAAGCAGCGG CTGCTGGTCC TCAGCAAGGG GCTTCAGGAA TATGAGGAAT 
     4441 GGAAATGGGG CAAGAACCCC ACAATGGTGG AGGTGCTGGA GGAGTTCCCG TCCATCCAGA 
     4501 TGCCGGCCAC ACTTCTCCTC ACACAGCTGT CGCTGCTACA ACCTCGCTAC TATTCCATCA 
     4561 GCTCCTCTCC AGACATGTAC CCCGACGAGG TGCACCTCAC CGTGGCCATC GTGTCCTACC 
     4621 ATACCCGAGA TGGAGAAGGA CCGGTTCACC ACGGGGTGTG CTCCTCCTGG CTCAACCGAA 
     4681 TACAGGCTGA CGATGTCGTC CCCTGCTTCG TGAGAGGTGC CCCTAGCTTC CACCTGCCTC 
     4741 GAAACCCCCA GGTGCCTTGC ATCCTGGTTG GCCCAGGCAC CGGCATCGCT CCCTTCCGAA 
     4801 GTTTTTGGCA ACAGCGACAA TTTGACATCC AACACAAAGG AATGAATCCG TGCCCCATGG 
     4861 TTCTGGTCTT CGGGTGTCGA CAATCCAAGA TAGATCATAT CTACAGAGAG GAGACCCTGC 
     4921 AGGCCAAGAA CAAGGGCGTC TTCAGAGAGC TGTACACTGC CTACTCCCGG GAACCCGACA 
     4981 GGCCAAAGAA ATATGTACAG GACGTGCTGC AGGAACAGCT GGCCGAGTCT GTGTACCGCG 
     5041 CCCTGAAGGA GCAAGGAGGC CATATTTATG TCTGCGGGGA CGTTACCATG GCCGCTGATG 
     5101 TCCTCAAAGC CATCCAGCGC ATAATGACCC AGCAGGGGAA ACTCTCGGAG GAGGATGCTG 
     5161 GTGTGTTCAT CAGCAGGCTG AGGGATGACA ACCGGTACCA CGAGGACATC TTTGGGGTCA 
     5221 CCCTCAGAAC ATATGAAGTG ACCAACCGCC TTAGATCTGA GTCCATCGCC TTCATTGAAG 
     5281 AGAGCAAAAA AGACACAGAT GAGGTTTTCA GCTCCACGCG TACGCGGCCG CTCGAGCAGA 
     5341 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5401 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5461 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5521 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5581 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5641 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5701 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5761 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5821 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5881 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5941 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6001 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6061 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6121 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6181 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6241 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6301 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6361 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6421 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6481 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6541 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6601 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6661 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6721 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6781 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6841 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6901 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6961 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7021 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7081 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7141 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7201 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7261 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7321 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7381 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7441 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7501 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7561 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7621 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7681 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7741 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7801 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7861 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7921 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7981 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8041 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8101 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8161 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8221 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8281 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8341 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8401 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8461 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8521 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8581 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8641 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8701 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8761 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8821 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8881 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8941 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9001 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9061 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9121 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//