LOCUS       MR227409                8988 bp    DNA     circular  SYN 
DEFINITION  Igf1r (Myc-DDK-tagged) - Mouse insulin-like growth factor I receptor (Igf1r)
ACCESSION   NM_010513
VERSION     NM_010513.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5145..5236 
                     /label="Myc-DDK"  
     misc_feature    5266..5851 
                     /label="PolyA signal"  
     misc_feature    5323..5342 
                     /label="Restriction Enzyme"  
     misc_feature    6127..6799 
                     /label="ColE1"  
     misc_feature    7229..8023 
                     /label="Kan/Neo"  
     misc_feature    8058..8360 
                     /label="SV40 ori"  
     misc_feature    8446..8923 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5127..5133 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5127 
                     /label="Igf1r"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTCTGGC TCCGGAGGAG GGTCCCCGAC CTCGCTGTGG GGGCTCGTGT 
     1081 TTCTCTCCGC CGCGCTCTCT CTCTGGCCGA CGAGTGGAGA AATCTGTGGG CCCGGCATTG 
     1141 ACATCCGCAA CGACTATCAG CAGCTGAAGC GCCTGGAAAA CTGCACGGTG ATCGAGGGCT 
     1201 TCCTCCACAT CCTGCTCATC TCCAAGGCCG AGGACTACCG AAGCTACCGC TTCCCCAAGC 
     1261 TCACCGTCAT CACTGAGTAC TTGCTGCTCT TCCGAGTCGC TGGCCTCGAG AGCCTGGGAG 
     1321 ACCTCTTCCC CAACCTCACA GTCATCCGTG GCTGGAAACT CTTCTACAAC TACGCACTGG 
     1381 TCATCTTCGA GATGACCAAT CTCAAGGATA TTGGGCTTTA TAATCTGAGG AACATTACTC 
     1441 GGGGGGCCAT CAGGATTGAG AAGAACGCCG ACCTCTGTTA CCTCTCCACC ATAGACTGGT 
     1501 CTCTCATCTT GGATGCGGTG TCCAATAACT ACATTGTGGG GAACAAGCCC CCGAAGGAAT 
     1561 GTGGGGACCT GTGTCCAGGG ACATTGGAGG AGAAGCCCAT GTGTGAGAAG ACCACCATCA 
     1621 ACAATGAGTA CAACTACCGC TGCTGGACCA CAAATCGCTG CCAGAAAATG TGCCCAAGTG 
     1681 TGTGCGGGAA GCGAGCCTGC ACCGAGAACA ACGAGTGCTG CCACCCGGAG TGCCTGGGCA 
     1741 GCTGCCACAC ACCGGACGAC AACACAACCT GCGTGGCCTG CAGACACTAC TACTACAAAG 
     1801 GCGTGTGTGT GCCTGCCTGC CCGCCTGGCA CCTACAGGTT CGAGGGCTGG CGCTGTGTGG 
     1861 ATCGCGATTT CTGCGCCAAC ATCCCCAACG CTGAGAGCAG TGACTCGGAT GGCTTCGTTA 
     1921 TCCACGACGA TGAGTGCATG CAGGAGTGTC CCTCAGGCTT CATCCGCAAC AGCACCCAGA 
     1981 GCATGTACTG TATCCCCTGC GAAGGCCCCT GCCCCAAAGT CTGCGGCGAT GAAGAGAAGA 
     2041 AAACGAAAAC CATCGATTCG GTGACTTCTG CTCAAATGCT CCAAGGATGC ACCATCCTGA 
     2101 AGGGCAATCT GCTTATTAAC ATCCGGAGAG GCAATAACAT TGCCTCGGAG TTGGAGAACT 
     2161 TCATGGGGCT CATCGAGGTG GTGACCGGCT ACGTGAAGAT CCGCCATTCT CATGCCTTGG 
     2221 TCTCCTTGTC CTTCCTGAAG AACCTTCGTC TCATCTTAGG AGAGGAGCAG CTGGAAGGGA 
     2281 ACTACTCCTT CTATGTCCTA GACAACCAGA ACTTGCAGCA GCTGTGGGAC TGGAACCACC 
     2341 GGAACCTGAC CGTCAGGTCC GGAAAGATGT ACTTTGCTTT CAATCCCAAG CTGTGTGTCT 
     2401 CCGAAATTTA CCGCATGGAG GAAGTGACCG GAACCAAGGG ACGCCAGAGC AAAGGGGACA 
     2461 TAAACACCAG GAACAACGGA GAGCGAGCTT CCTGTGAAAG TGATGTTCTC CGTTTCACCT 
     2521 CCACCACGAC CTGGAAGAAC CGAATCATCA TAACGTGGCA CCGGTACCGG CCGCCGGACT 
     2581 ACCGGGATCT CATCAGCTTC ACAGTTTACT ACAAGGAGGC ACCATTTAAA AACGTTACGG 
     2641 AATATGACGG GCAGGATGCC TGTGGCTCCA ACAGCTGGAA CATGGTGGAT GTAGACCTGC 
     2701 CTCCGAACAA GGAGGGCGAG CCTGGCATTT TACTGCATGG GCTGAAGCCC TGGACCCAGT 
     2761 ATGCTGTCTA TGTCAAGGCT GTGACCCTCA CCATGGTGGA AAACGACCAT ATCCGTGGGG 
     2821 CCAAAAGTGA AATCTTGTAC ATTCGCACCA ATGCTTCAGT CCCTTCCATT CCCCTAGATG 
     2881 TCCTCTCAGC ATCAAACTCT TCCTCTCAGC TGATTGTGAA GTGGAATCCT CCAACTCTGC 
     2941 CCAATGGTAA CTTGAGTTAC TACATTGTGA GGTGGCAGCG GCAGCCCCAG GATGGTTACC 
     3001 TGTACCGGCA CAACTACTGC TCCAAAGACA AAATACCCAT CAGAAAGTAC GCCGATGGTA 
     3061 CCATCGACGT GGAGGAGGTG ACGGAAAATC CCAAGACAGA AGTGTGTGGT GGTGATAAAG 
     3121 GGCCATGCTG CGCTTGCCCT AAAACTGAAG CTGAGAAGCA GGCTGAGAAG GAGGAGGCTG 
     3181 AGTACCGTAA AGTCTTTGAG AATTTCCTTC ACAATTCCAT CTTTGTGCCC AGGCCCGAAA 
     3241 GGAGGCGGAG AGACGTCATG CAAGTGGCCA ACACGACCAT GTCCAGCCGA AGCAGGAACA 
     3301 CCACGGTAGC TGACACCTAC AATATCACAG ACCCGGAGGA GTTCGAGACA GAGTACCCTT 
     3361 TCTTTGAGAG CAGAGTGGAT AACAAGGAGA GGACTGTCAT CTCCAACCTC CGGCCTTTCA 
     3421 CTCTGTACCG CATCGATATC CACAGCTGCA ACCACGAGGC TGAGAAGCTG GGCTGCAGCG 
     3481 CCTCCAACTT CGTCTTTGCG AGAACCATGC CAGCAGAAGG AGCAGATGAT ATCCCTGGTC 
     3541 CGGTGACCTG GGAGCCAAGA CCCGAAAACT CCATCTTTTT AAAGTGGCCA GAACCCGAGA 
     3601 ACCCCAACGG ATTGATCCTA ATGTATGAAA TTAAATACGG GTCGCAAGTC GAGGATCAGC 
     3661 GGGAATGTGT GTCCAGACAG GAGTACAGGA AGTACGGAGG GGCCAAACTC AACCGTCTAA 
     3721 ACCCAGGGAA CTATACAGCC CGGATTCAGG CTACCTCCCT CTCTGGGAAT GGGTCATGGA 
     3781 CAGATCCTGT GTTCTTCTAT GTCCCCGCCA AAACGACGTA TGAGAACTTC ATGCATCTGA 
     3841 TCATTGCTCT GCCGGTTGCC ATCCTGCTGA TCGTTGGGGG GCTGGTTATC ATGCTGTATG 
     3901 TCTTCCATAG AAAGAGAAAT AACAGCAGGT TGGGCAATGG AGTGCTGTAT GCTTCTGTGA 
     3961 ACCCCGAGTA TTTCAGCGCA GCTGATGTGT ACGTGCCTGA TGAATGGGAG GTAGCTCGAG 
     4021 AGAAGATCAC CATGAACCGG GAGCTCGGAC AAGGGTCCTT TGGGATGGTC TATGAAGGAG 
     4081 TGGCCAAGGG TGTGGTCAAG GATGAACCCG AAACCAGAGT GGCCATCAAG ACGGTAAACG 
     4141 AGGCTGCAAG TATGCGTGAA AGAATCGAGT TTCTCAACGA GGCCTCGGTG ATGAAGGAGT 
     4201 TCAATTGTCA CCATGTGGTC CGGTTGCTGG GTGTGGTATC CCAAGGCCAG CCCACCCTGG 
     4261 TCATCATGGA ACTAATGACA CGCGGTGATC TCAAAAGTTA TCTCCGGTCT CTGAGGCCAG 
     4321 AAGTGGAGCA GAATAATCTA GTCCTCATTC CTCCGAGCTT AAGCAAGATG ATCCAGATGG 
     4381 CTGGAGAGAT TGCAGATGGC ATGGCCTACC TCAATGCCAA CAAGTTCGTC CACAGAGACC 
     4441 TTGCTGCTAG GAACTGCATG GTAGCCGAAG ATTTCACAGT CAAAATTGGA GATTTCGGTA 
     4501 TGACACGAGA CATCTACGAG ACGGACTACT ACCGGAAAGG CGGGAAGGGG TTGCTGCCTG 
     4561 TGCGCTGGAT GTCTCCCGAG TCCCTCAAGG ATGGTGTCTT CACTACTCAT TCTGATGTCT 
     4621 GGTCCTTCGG GGTCGTCCTC TGGGAGATCG CCACGCTGGC TGAGCAGCCC TACCAGGGCT 
     4681 TGTCCAACGA GCAAGTTCTT CGTTTCGTCA TGGAGGGTGG CCTTCTGGAC AAGCCGGACA 
     4741 ACTGCCCTGA TATGCTGTTT GAACTTATGC GCATGTGCTG GCAGTATAAC CCCAAGATGC 
     4801 GGCCCTCCTT CCTGGAGATC ATCGGCAGCA TCAAGGATGA GATGGAGCCC AGCTTCCAGG 
     4861 AGGTCTCCTT CTACTACAGC GAGGAGAACA AGCCTCCCGA GCCAGAGGAG CTGGAGATGG 
     4921 AGCCTGAGAA CATGGAGAGC GTCCCACTGG ACCCTTCGGC CTCCTCAGCC TCCCTGCCTC 
     4981 TGCCTGAAAG ACACTCAGGA CACAAGGCTG AGAATGGCCC GGGCCCTGGC GTGCTCGTTC 
     5041 TCCGCGCCAG TTTTGATGAG AGACAGCCTT ACGCTCACAT GAACGGGGGA CGCGCCAACG 
     5101 AGAGGGCCTT GCCTCTGCCC CAGTCCTCGA CCTGCACGCG TACGCGGCCG CTCGAGCAGA 
     5161 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5221 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5281 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5341 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5401 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5461 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5521 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5581 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5641 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5701 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5761 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5821 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5881 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5941 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6001 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6061 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6121 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6181 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6241 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6301 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6361 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6421 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6481 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6541 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6601 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6661 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6721 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6781 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6841 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6901 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6961 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7021 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7081 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7141 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7201 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7261 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7321 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7381 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7441 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7501 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7561 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7621 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7681 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7741 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7801 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7861 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7921 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7981 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8041 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8101 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8161 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8221 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8281 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8341 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8401 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8461 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8521 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8581 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8641 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8701 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8761 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8821 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8881 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8941 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//