LOCUS       MR227391                8490 bp    DNA     circular  SYN 
DEFINITION  Grm5 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 5 (Grm5), transcript variant b
ACCESSION   NM_001143834
VERSION     NM_001143834.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4647..4738 
                     /label="Myc-DDK"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    4825..4844 
                     /label="Restriction Enzyme"  
     misc_feature    5629..6301 
                     /label="ColE1"  
     misc_feature    6731..7525 
                     /label="Kan/Neo"  
     misc_feature    7560..7862 
                     /label="SV40 ori"  
     misc_feature    7948..8425 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="Grm5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCTTCTG TTGATTCTGT CAGTCCTACT TCTGAAAGAA GATGTACGAG 
     1081 GAAGTGCACA GTCCAGTGAG AGGAGGGTGG TGGCTCACAT GCCAGGTGAC ATTATTATTG 
     1141 GAGCTCTCTT CTCGGTCCAC CACCAACCAA CTGTGGACAA AGTTCATGAG AGGAAGTGTG 
     1201 GGGCAGTCCG TGAGCAGTAT GGCATTCAGA GAGTGGAAGC CATGCTGCAT ACCTTGGAAA 
     1261 GGATCAATTC AGATCCCACA CTCTTGCCCA ACATCACACT AGGCTGTGAG ATAAGAGATT 
     1321 CCTGCTGGCA TTCTGCTGTG GCCCTAGAGC AAAGCATTGA GTTTATAAGG GATTCCCTCA 
     1381 TCTCTTCGGA AGAGGAAGAA GGCTTGGTAC GCTGTGTAGA TGGCTCTTCT TCCTTCCGCT 
     1441 CCAAGAAACC CATAGTGGGA GTCATTGGGC CTGGCTCGAG TTCTGTGGCC ATTCAAGTTC 
     1501 AGAACTTGCT CCAGCTTTTC AACATACCTC AGATTGCTTA CTCTGCAACT AGCATGGATT 
     1561 TGAGTGACAA GACTCTATTC AAGTACTTCA TGAGGGTTGT ACCTTCGGAT GCCCAGCAAG 
     1621 CCCGAGCCAT GGTAGACATA GTGAAGAGAT ACAACTGGAC TTATGTCTCA GCTGTGCACA 
     1681 CAGAAGGCAA CTATGGAGAA AGTGGGATGG AGGCTTTCAA AGATATGTCA GCGAAGGAAG 
     1741 GGATTTGCAT CGCCCACTCT TACAAAATCT ACAGCAATGC TGGGGAACAG AGCTTTGACA 
     1801 AGCTGTTGAA AAAGCTCAGA AGTCATTTAC CTAAAGCCCG GGTGGTAGCC TGCTTCTGTG 
     1861 AAGGCATGAC AGTTCGAGGT CTGCTCATGG CCATGAGACG CTTGGGTCTA GCAGGGGAAT 
     1921 TTCTACTTCT GGGCAGTGAT GGCTGGGCTG ACAGGTATGA CGTGACAGAT GGGTATCAGC 
     1981 GAGAAGCTGT CGGTGGGATT ACAATCAAGC TCCAGTCTCC TGATGTCAAG TGGTTTGATG 
     2041 ATTATTATCT GAAGCTCCGG CCAGAAACAA ACCTCAGAAA CCCTTGGTTT CAAGAATTTT 
     2101 GGCAGCATCG TTTTCAGTGC CGGCTAGAAG GGTTTGCACA GGAGAACAGC AAGTACAACA 
     2161 AGACTTGCAA CAGTTCTCTA ACTCTGAGAA CGCATCATGT TCAAGATTCC AAAATGGGAT 
     2221 TTGTGATCAA TGCAATCTAT TCTATGGCTT ATGGGCTCCA CAACATGCAG ATGTCCCTGT 
     2281 GTCCAGGCTA TGCAGGCCTC TGTGATGCAA TGAAGCCAAT TGATGGGCGG AAACTTTTGG 
     2341 ACTCCCTGAT GAAAACCAAC TTTACTGGAG TTTCCGGAGA TATGATTCTA TTTGATGAAA 
     2401 ATGGAGACTC TCCAGGAAGG TATGAAATAA TGAATTTCAA GGAAATGGGA AAAGATTATT 
     2461 TTGATTACAT CAATGTTGGA AGTTGGGACA ATGGGGAATT AAAAATGGAT GATGATGAAG 
     2521 TGTGGTCCAA GAAAAATAAC ATCATCAGAT CTGTGTGCAG TGAACCGTGT GAGAAAGGAC 
     2581 AGATAAAGGT GATCCGGAAG GGAGAAGTCA GCTGTTGTTG GACCTGCACA CCTTGTAAGG 
     2641 AGAATGAGTA TGTTTTTGAT GAGTACACCT GCAAGGCGTG CCAACTGGGG TCCTGGCCCA 
     2701 CTGACGACTT GACAGGTTGT GATTTGATCC CAGTCCAGTA TCTTCGATGG GGTGACCCTG 
     2761 AGCCCATTGC AGCTGTGGTG TTTGCCTGCC TCGGTCTGCT AGCCACCCTC TTCGTTACTG 
     2821 TAATCTTCAT CATTTATCGG GACACTCCAG TGGTCAAGTC TTCCAGCAGG GAACTCTGCT 
     2881 ACATTATCCT TGCTGGCATC TGCCTGGGTT ACTTGTGTAC CTTCTGCCTC ATTGCAAAGC 
     2941 CCAAACAGAT TTACTGCTAC CTTCAGAGAA TTGGCATCGG TCTCTCTCCA GCCATGAGCT 
     3001 ACTCAGCCCT TGTAACGAAG ACCAACCGTA TTGCAAGGAT TCTAGCTGGC AGCAAGAAGA 
     3061 AGATCTGTAC CAAAAAGCCC AGATTCATGA GCGCCTGTGC TCAGTTAGTG ATCGCTTTCA 
     3121 TTCTCATCTG TATACAGTTG GGTATTATTG TGGCCCTCTT TATCATGGAG CCTCCGGATA 
     3181 TAATGCATGA CTATCCAAGC ATCCGAGAAG TCTACTTGAT TTGTAACACC ACCAACCTAG 
     3241 GGGTTGTCAC TCCTCTTGGA TACAATGGAT TATTGATTTT GAGTTGCACA TTCTATGCGT 
     3301 TCAAGACCAG AAATGTTCCA GCCAACTTTA ACGAGGCCAA ATATATTGCT TTCACCATGT 
     3361 ACACAACCTG CATCATATGG CTGGCCTTTG TGCCTATCTA CTTTGGCAGC AACTACAAAA 
     3421 TCATCACCAT GTGTTTCTCA GTCAGCCTCA GTGCCACAGT GGCCCTGGGT TGCATGTTTG 
     3481 TGCCGAAGGT GTACATCATC CTAGCCAAAC CGGAGAGAAA TGTGCGCAGC GCCTTCACAA 
     3541 CCTCTACAGT GGTGCGCATG CACGTAGGAG ATGGCAAGTC ATCATCCGCT GCCAGCAGAT 
     3601 CCAGCAGCCT AGTCAACCTG TGGAAGAGGA GGGGCTCGTC TGGGGAAACC CTAAGGTACA 
     3661 AAGACAGGAG ACTGGCCCAG CACAAGTCGG AAATAGAGTG TTTCACCCCC AAAGGGAGTA 
     3721 TGGGGAATGG TGGGAGAGCA ACAATGAGCA GCTCCAATGG AAAATCCGTG ACTTGGGCCC 
     3781 AGAATGAGAA GAGCACCCGG GGGCAGCACC TGTGGCAGCG ACTGTCTGTC CATATCAACA 
     3841 AGAAGGAGAA CCCCAACCAG ACAGCAGTCA TCAAACCCTT CCCCAAGAGC ACAGAGAGCC 
     3901 GCGGGCAGGG TGCAGGGGCA GGTGGTGGCT CTGGCCCCGG TGCAGCTGGT GCTGGTAGCG 
     3961 CAGGATGCAC AGCGACAGGC GGCCCAGAGC CACCAGACGC CGGCCCCAAG GCGCTTTATG 
     4021 ATGTCGCAGA GGCAGAGGAG CGCTTCCCAG CGGCTGCCAG GCCGCGCTCG CCATCGCCCA 
     4081 TCAGTACGCT GAGCCACCTG GCAGGCTCGG CGGGCCGCAC AGACGACGAC GCGCCGTCGC 
     4141 TGCACTCGGA GACCGCTGCA CGCAGCAGCT CATCCCAGGG CTCGCTCATG GAGCAGATTA 
     4201 GCAGCGTGGT GACGCGCTTC ACCGCCAACA TCACCGAGCT CAACTCCATG ATGTTGTCCA 
     4261 CCGCGGCTGC GCCGGGGCCC CCTGGTACCC CTATCTGCTC TTCCTACCTG ATCCCCAAAG 
     4321 AGATCCAGCT GCCCACGACC ATGACGACCT TCGCAGAGAT CCAGCCTCTG CCGGCCATCG 
     4381 AGGTGACCGG AGGAGCTCAG CCGGCGACAG GGCCATCACC TGCCCAAGAG ACGCCCGCAG 
     4441 GAGCTGAAGC CGCCCCAGGA AAACCGGATC TGGAGGAGCT GGTGGCCCTC ACTCCACCAT 
     4501 CGCCCTTCAG GGACTCGGTG GACTCGGGGA GCACCACCCC AAACTCTCCA GTCTCCGAAT 
     4561 CGGCCCTCTG CATCCCATCC TCTCCCAAAT ATGACACTCT CATCATCAGA GATTACACGC 
     4621 AGAGTTCTTC ATCGTTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//