LOCUS       MR227371                7311 bp    DNA     circular  SYN 
DEFINITION  Il4ra (Myc-DDK-tagged) - Mouse interleukin 4 receptor, alpha (Il4ra)
ACCESSION   NM_001008700
VERSION     NM_001008700.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3468..3559 
                     /label="Myc-DDK"  
     misc_feature    3589..4174 
                     /label="PolyA signal"  
     misc_feature    3646..3665 
                     /label="Restriction Enzyme"  
     misc_feature    4450..5122 
                     /label="ColE1"  
     misc_feature    5552..6346 
                     /label="Kan/Neo"  
     misc_feature    6381..6683 
                     /label="SV40 ori"  
     misc_feature    6769..7246 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3450..3456 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3450 
                     /label="Il4ra"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGGCTT TGCACCAAGT TCCTGACCTC TGTGGGCTGT CTGATTTTGC 
     1081 TGTTGGTGAC TGGATCTGGG AGCATCAAGG TCCTGGGTGA GCCCACCTGC TTCTCTGACT 
     1141 ACATCCGCAC TTCCACGTGT GAGTGGTTCC TGGATAGCGC TGTGGACTGC AGTTCTCAGC 
     1201 TCTGCCTACA CTACAGGCTG ATGTTCTTCG AGTTCTCTGA AAACCTCACA TGCATCCCGA 
     1261 GGAACAGTGC CAGCACTGTG TGTGTGTGCC ACATGGAAAT GAATAGGCCG GTCCAATCAG 
     1321 ACAGATACCA GATGGAACTG TGGGCTGAGC ACAGACAGCT GTGGCAGGGC TCCTTCAGCC 
     1381 CCAGTGGTAA TGTGAAGCCC CTAGCTCCAG ACAACCTCAC ACTCCACACC AATGTGTCCG 
     1441 ACGAATGGCT GCTGACCTGG AATAACCTGT ACCCATCGAA CAACTTACTG TACAAAGACC 
     1501 TCATCTCCAT GGTCAACATC TCCAGAGAGG ACAACCCTGC AGAATTCATA GTCTATAATG 
     1561 TGACCTACAA GGAACCCAGG CTGAGCTTCC CGATCAACAT CCTGATGTCA GGGGTCTACT 
     1621 ATACGGCGCG TGTGAGGGTC AGATCCCAGA TACTCACTGG CACCTGGAGT GAGTGGAGTC 
     1681 CTAGCATCAC GTGGTACAAC CACTTCCAGC TGCCCCTGAT ACAGCGCCTT CCACTGGGGG 
     1741 TCACCATCTC CTGCCTCTGC ATCCCGTTGT TTTGCCTGTT CTGTTACTTC AGCATTACCA 
     1801 AGATTAAGAA GATATGGTGG GACCAGATTC CCACCCCAGC ACGCAGTCCC TTGGTGGCCA 
     1861 TCATCATTCA GGATGCACAG GTGCCCCTCT GGGATAAGCA GACCCGAAGC CAGGAGTCAA 
     1921 CCAAGTACCC GCACTGGAAA ACTTGTCTAG ACAAGCTGCT GCCTTGCTTG CTGAAGCACA 
     1981 GAGTAAAGAA GAAGACAGAC TTCCCGAAGG CTGCCCCAAC CAAGTCTCTC CAGAGTCCTG 
     2041 GAAAGGCAGG CTGGTGTCCC ATGGAGGTCA GCAGGACCGT CCTCTGGCCA GAGAATGTTA 
     2101 GTGTCAGTGT GGTGCGCTGT ATGGAGCTGT TTGAGGCCCC AGTACAGAAT GTGGAGGAGG 
     2161 AAGAAGATGA GATAGTCAAA GAGGACCTGA GCATGTCACC TGAGAACAGC GGAGGCTGCG 
     2221 GCTTCCAGGA GAGCCAGGCA GACATCATGG CTCGGCTCAC TGAGAACCTG TTTTCCGACT 
     2281 TGTTGGAGGC TGAGAATGGG GGCCTTGGCC AGTCAGCCTT GGCAGAGTCA TGCTCCCCTC 
     2341 TGCCTTCAGG AAGTGGGCAG GCTTCTGTAT CCTGGGCCTG CCTCCCCATG GGGCCCAGTG 
     2401 AGGAGGCCAC ATGCCAGGTC ACAGAGCAGC CTTCACACCC AGGCCCTCTT TCAGGCAGCC 
     2461 CAGCCCAGAG TGCACCTACT CTGGCTTGCA CGCAGGTCCC ACTTGTCCTT GCAGACAATC 
     2521 CTGCCTACCG GAGTTTTAGT GACTGCTGTA GCCCGGCCCC AAATCCTGGA GAGCTGGCTC 
     2581 CAGAGCAGCA GCAGGCTGAT CATCTGGAAG AAGAGGAGCC TCCAAGCCCG GCTGACCCCC 
     2641 ATTCTTCAGG GCCACCAATG CAGCCAGTGG AGAGCTGGGA GCAGATCCTT CACATGAGTG 
     2701 TCCTGCAGCA TGGGGCAGCT GCTGGCTCCA CCCCAGCCCC TGCCGGTGGC TACCAGGAGT 
     2761 TTGTGCAGGC AGTGAAGCAG GGTGCCGCCC AGGATCCTGG GGTGCCTGGT GTCAGGCCTT 
     2821 CTGGAGACCC CGGTTACAAG GCCTTCTCGA GCCTGCTCAG CAGCAATGGC ATCCGCGGGG 
     2881 ACACAGCAGC AGCGGGGACT GACGATGGGC ATGGAGGCTA CAAGCCCTTC CAGAATCCTG 
     2941 TTCCTAACCA GTCCCCTAGC TCCGTGCCCT TATTTACTTT CGGACTAGAC ACGGAGCTGT 
     3001 CACCCAGTCC TCTGAACTCA GACCCACCCA AAAGCCCCCC AGAATGCCTT GGTCTGGAGC 
     3061 TGGGGCTCAA AGGAGGTGAC TGGGTGAAGG CCCCTCCTCC TGCAGATCAG GTGCCCAAGC 
     3121 CCTTTGGGGA TGACCTGGGC TTTGGTATTG TGTACTCGTC CCTCACTTGC CACTTGTGTG 
     3181 GCCACCTGAA GCAACACCAC AGCCAGGAGG AAGGTGGCCA GAGCCCCATC GTTGCTAGCC 
     3241 CTGGCTGTGG CTGCTGCTAC GATGACAGAT CACCATCCCT GGGGAGCCTC TCGGGGGCCT 
     3301 TGGAAAGCTG TCCTGAGGGA ATACCACCAG AAGCCAACCT CATGTCAGCA CCCAAGACAC 
     3361 CCTCAAACTT GTCAGGGGAG GGCAAGGGCC CTGGTCACTC TCCTGTTCCC AGCCAGACGA 
     3421 CCGAGGTGCC TGTGGGCGCC CTGGGCATTG CTGTTTCTAC GCGTACGCGG CCGCTCGAGC 
     3481 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3541 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3601 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3661 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3721 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3781 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3841 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3901 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3961 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4021 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4081 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4141 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4201 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4261 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4321 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4381 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4441 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4501 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4561 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4621 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4681 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4741 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4801 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4861 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4921 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4981 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5041 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5101 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5161 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5221 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5281 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5341 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5401 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5461 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5521 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5581 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5641 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5701 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5761 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5821 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5881 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5941 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6001 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6061 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6121 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6181 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6241 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6301 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6361 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6421 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6481 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6541 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6601 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6661 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6721 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6781 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6841 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6901 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6961 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7021 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7081 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7141 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7201 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7261 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//