LOCUS       MR227326                8343 bp    DNA     circular  SYN 
DEFINITION  Itgam (Myc-DDK-tagged) - Mouse integrin alpha M (Itgam), transcript variant 1
ACCESSION   NM_001082960
VERSION     NM_001082960.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4500..4591 
                     /label="Myc-DDK"  
     misc_feature    4621..5206 
                     /label="PolyA signal"  
     misc_feature    4678..4697 
                     /label="Restriction Enzyme"  
     misc_feature    5482..6154 
                     /label="ColE1"  
     misc_feature    6584..7378 
                     /label="Kan/Neo"  
     misc_feature    7413..7715 
                     /label="SV40 ori"  
     misc_feature    7801..8278 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4482..4488 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4482 
                     /label="Itgam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCTTAAA GCTCTTCTGG TCACAGCCCT AGCCTTGTGT CATGGCTTCA 
     1081 ATCTGGACAC TGAACATCCC ATGACCTTCC AAGAGAATGC AAAAGGCTTT GGACAGAGTG 
     1141 TGGTCCAGCT TGGCGGAACC AGTGTGGTTG TTGCAGCCCC CCAGGAGGCA AAGGCTGTTA 
     1201 ACCAGACAGG TGCCCTCTAC CAGTGTGACT ACAGCACAAG CCGGTGTCAC CCCATCCCCC 
     1261 TGCAAGTACC TCCAGAGGCT GTGAATATGT CCTTGGGCCT GTCCCTGGCT GTTTCTACTG 
     1321 TCCCCCAGCA GCTGCTGGCC TGTGGCCCCA CGGTGCACCA AAACTGCAAG GAGAATACTT 
     1381 ATGTGAATGG ATTGTGCTAT TTGTTCGGCT CCAACCTGCT GAGGCCGCCC CAGCAGTTCC 
     1441 CAGAGGCTCT CAGAGAATGT CCTCAGCAGG AGAGTGACAT TGTCTTCTTG ATTGATGGCT 
     1501 CCGGTAGCAT CAACAACATT GACTTTCAGA AGATGAAGGA GTTTGTCTCA ACTGTGATGG 
     1561 AGCAGTTCAA AAAGTCTAAA ACCTTGTTCT CTTTGATGCA GTACTCGGAC GAGTTCCGGA 
     1621 TTCACTTCAC CTTCAATGAC TTCAAGAGAA ACCCTAGCCC AAGATCACAT GTGAGCCCCA 
     1681 TAAAGCAGCT GAATGGGAGG ACAAAAACTG CCTCAGGGAT CCGGAAAGTA GTGAGAGAAC 
     1741 TGTTTCACAA AACCAATGGG GCCCGGGAGA ATGCTGCGAA GATCCTAGTT GTCATCACAG 
     1801 ATGGAGAAAA ATTCGGTGAT CCCTTGGATT ATAAGGATGT CATCCCCGAG GCAGACAGAG 
     1861 CAGGGGTCAT TCGCTACGTA ATTGGGGTGG GAAATGCCTT CAACAAACCA CAGTCCCGCA 
     1921 GAGAGCTCGA CACCATCGCA TCTAAGCCAG CTGGTGAACA CGTGTTCCAA GTGGACAACT 
     1981 TTGAAGCCCT GAATACCATT CAGAACCAGC TTCAGGAAAA GATCTTTGCA ATTGAGGGCA 
     2041 CGCAGACAGG AAGTACCAGC TCCTTTGAGC ATGAGATGTC TCAAGAAGGC TTCAGTGCTT 
     2101 CCATTACCTC TAATGGTCCC TTGCTGGGCT CTGTGGGGAG CTTTGACTGG GCAGGTGGAG 
     2161 CCTTCCTGTA TACATCGAAG GATAAAGTCA CCTTCATCAA CACAACCAGA GTGGATTCAG 
     2221 ACATGAATGA TGCTTACCTG GGTTATGCTT CTGCAGTCAT CTTGAGGAAC CGTGTCCAAA 
     2281 GCTTGGTTTT AGGAGCACCT CGGTATCAGC ATATTGGCTT GGTGGTGATG TTCAGGGAGA 
     2341 ATTTCGGTAC CTGGGAGCCC CACACTAGCA TCAAGGGCAG CCAGATTGGC TCTTATTTTG 
     2401 GGGCCTCCCT TTGCTCTGTG GACATGGACG CTGATGGCAA TACCAACTTG ATCCTCATTG 
     2461 GGGCCCCTCA TTACTATGAG AAGACCCGAG GAGGCCAGGT GTCAGTGTGT CCCTTGCCTC 
     2521 GAGGGCAGAG GGCACGGTGG CAGTGTGAAG CTCTTCTCCA CGGTGATCAG GGTCATCCCT 
     2581 GGGGTCGCTT TGGGGCAGCC CTGACAGTGC TGGGAGACGT GAATGGGGAC AAACTGACAG 
     2641 ATGTGGCCAT TGGGGCCCCA GGAGAGCAGG AGAATCAGGG CGCTGTCTAC ATTTTTTATG 
     2701 GAGCATCAAT AGCCAGCCTC AGTGCCTCCC ACAGCCAGCG GATCATAGGC GCCCACTTCT 
     2761 CCCCTGGGCT CCAGTACTTC GGGCAGTCTC TGAGTGGGGG TAAGGATCTC ACAATGGATG 
     2821 GCTTGATGGA CCTGGCTGTT GGGGCGCAGG GGCATCTTCT GCTGCTCAGA GCCCAGCCGG 
     2881 TGCTGAGACT GGAGGCAACC ATGGAATTCA GCCCCAAGAA AGTAGCAAGG AGTGTGTTTG 
     2941 CATGTCAAGA ACAAGTATTA AAAAACAAGG ATGCTGGGGA GGTCAGAGTC TGCCTCCGTG 
     3001 TCCGCAAGAA CACCAAGGAC CGTCTGCGCG AAGGAGATAT CCAGAGCACT GTCACTTATG 
     3061 ACCTGGCTTT AGACCCTGGC CGCTCACGTA TCCGTGCCTT CTTTGATGAG ACAAAGAACA 
     3121 ACACACGCAG GCGCACCCAG GTCTTTGGAT TGATGCAGAA ATGTGAAACA CTGAAGCTAA 
     3181 TTTTACCGGA CTGCGTGGAC GACTCAGTGA GCCCCATCAT CCTGCGCCTC AATTATACAC 
     3241 TGGTTGGGGA GCCCTTGAGG TCCTTTGGGA ACCTCCGACC AGTTCTGGCT ATGGATGCTC 
     3301 AGAGGTTCTT CACAGCTATG TTTCCCTTTG AGAAAAATTG CGGCAATGAC AGCATCTGCC 
     3361 AAGACGATCT CAGCATCACC ATGAGTGCCA TGGGCTTGGA CACTTTGGTG GTGGGAGGCC 
     3421 CCCAGGACTT TAACATGAGT GTGACTCTGA GAAATGACGG TGAGGATTCC TACGGGACCC 
     3481 AGGTTACCGT CTACTACCCA TCTGGCTTAT CTTACCGGAA GGATTCAGCA AGCCAGAACC 
     3541 CGCTCACCAA GAAGCCTTGG TTTGTAAAGC CTGCTGAGTC CAGCTCTTCT TCTGAAGGGC 
     3601 ATGGGGCTCT GAAGAGTACC ACCTGGAACA TAAACCATCC CATCTTTCCT GCTAATTCTG 
     3661 AGGTCACATT TAATGTCACA TTTGATGTGG ACTCTCATGC CTCCTTTGGG AACAAACTGC 
     3721 TCCTCAAAGC CATTGTGGCC AGTGAGAACA ACATGTCCAG GACCCACAAA ACCAAGTTTC 
     3781 AGTTGGAGCT GCCTGTGAAG TACGCCATCT ACATGATTGT CACCAGTGAT GAGAGTTCTA 
     3841 TCAGATATCT CAACTTCACG GCTTCAGAGA TGACCAGTAA GGTCATACAG CATCAGTACC 
     3901 AGTTCAACAA CCTGGGCCAG AGGAGCCTCC CTGTCAGTGT AGTCTTCTGG ATCCCTGTTC 
     3961 AGATCAACAA TGTGACCGTA TGGGATCATC CCCAGGTCAT CTTCTCCCAG AACCTCTCAA 
     4021 GTGCCTGTCA CACTGAGCAG AAATCCCCCC CTCACTCCAA TTTCCGGGAT CAGCTTGAAA 
     4081 GGACCCCAGT GCTGAACTGC TCTGTTGCAG TCTGTAAGAG AATTCAGTGT GACCTTCCAT 
     4141 CCTTCAACAC TCAGGAAATA TTCAATGTCA CCCTCAAGGG CAACCTATCA TTTGACTGGT 
     4201 ACATCAAGAC TTCTCATGGT CACCTCCTGC TTGTGAGCAG CACTGAGATC CTGTTTAATG 
     4261 ACTCTGCGTT TGCCCTGCTT CCAGGGCAGG AGTCGTATGT GAGGTCTAAG ACAGAGACCA 
     4321 AAGTGGAGCC ATATGAAGTT CACAATCCTG TACCACTCAT TGTGGGCAGC TCCATTGGGG 
     4381 GCCTGGTGCT CTTGGCTCTC ATCACTGCTG GCCTATACAA GCTTGGCTTT TTCAAGCGGC 
     4441 AGTACAAGGA CATGATGAAT GAAGCTGCCC CCCAAGACGC CCCACCTCAG ACGCGTACGC 
     4501 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4561 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4621 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4681 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4741 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4801 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4861 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4921 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4981 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5041 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5101 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5161 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5221 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5281 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5341 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5401 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5461 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5521 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5581 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5641 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5701 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5761 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5821 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5881 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5941 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6001 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6061 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6121 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6181 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6241 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6301 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6361 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6421 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6481 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6541 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6601 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6661 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6721 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6781 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6841 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6901 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6961 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7021 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7081 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7141 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7201 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7261 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7321 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7381 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7441 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7501 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7561 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7741 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7801 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7861 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7921 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7981 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8041 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8101 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8161 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8221 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8281 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8341 TTT
//