LOCUS       MR227208                8010 bp    DNA     circular  SYN 
DEFINITION  Rag1 (Myc-DDK-tagged) - Mouse recombination activating gene 1 (Rag1)
ACCESSION   NM_009019
VERSION     NM_009019.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4165..4256 
                     /label="Myc-DDK"  
     misc_feature    4286..4871 
                     /label="PolyA signal"  
     misc_feature    4343..4362 
                     /label="Restriction Enzyme"  
     misc_feature    5147..5819 
                     /label="ColE1"  
     misc_feature    6249..7043 
                     /label="Kan/Neo"  
     misc_feature    7078..7380 
                     /label="SV40 ori"  
     misc_feature    7466..7943 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4140..4147 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4140 
                     /label="Rag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCTCC TTGCCGTCTA CCCTGAGCTT CAGTTCTGCA CCCGATGAAA 
     1081 TTCAACACCC ACAAATCAAA TTTTCCGAGT GGAAATTTAA GCTGTTTAGG GTGAGATCCT 
     1141 TTGAAAAGGC ACCCGAAGAA GCACAGAAGG AGAAGGATTC CTCAGAGGGG AAACCTTACC 
     1201 TAGAACAGTC TCCAGTAGTT CCAGAGAAGC CTGGTGGTCA GAACTCAATT CTGACTCAAC 
     1261 GAGCACTGAA ACTCCATCCT AAATTTTCAA AGAAATTCCA TGCTGATGGG AAGTCAAGCG 
     1321 ACAAAGCAGT TCACCAAGCC AGGCTTAGAC ACTTCTGCCG CATCTGTGGG AATCGTTTCA 
     1381 AGAGTGACGG GCACAGCCGG AGATACCCAG TCCACGGGCC CGTGGACGCT AAAACCCAAA 
     1441 GTCTTTTCCG AAAGAAGGAA AAAAGAGTCA CTTCCTGGCC AGACCTCATT GCCAGGATTT 
     1501 TCCGGATCGA CGTGAAGGCA GATGTTGACT CCATCCACCC GACGGAATTC TGCCATGACT 
     1561 GTTGGAGCAT CATGCACAGA AAGTTCAGCA GTTCCCACAG TCAGGTCTAC TTCCCAAGGA 
     1621 AAGTGACCGT GGAGTGGCAC CCCCACACAC CGTCCTGTGA CATCTGTTTT ACTGCCCATC 
     1681 GGGGACTCAA GAGGAAGAGA CATCAGCCCA ATGTGCAGCT CAGCAAGAAA CTAAAAACTG 
     1741 TGCTCAACCA CGCGAGACGG GACCGTCGCA AGAGAACTCA GGCTAGGGTC AGCAGCAAGG 
     1801 AAGTCCTGAA GAAGATCTCC AACTGCAGTA AGATTCATCT CAGTACCAAG CTTCTTGCCG 
     1861 TGGACTTCCC AGCACACTTT GTGAAATCCA TCTCCTGCCA GATATGCGAA CACATTCTGG 
     1921 CTGATCCCGT GGAGACCAGC TGCAAGCATC TATTCTGTAG GATCTGCATT CTCAGATGTC 
     1981 TCAAAGTCAT GGGCAGCTAT TGTCCCTCTT GCCGATATCC GTGCTTCCCT ACTGACCTGG 
     2041 AGAGCCCAGT GAAGTCCTTT CTGAACATCT TGAATTCTCT CATGGTCAAG TGTCCCGCGC 
     2101 AAGATTGCAA TGAGGAAGTG AGTCTGGAAA AATATAACCA CCATGTGTCA AGCCACAAAG 
     2161 AATCTAAAGA GACTTTGGTG CATATCAATA AAGGGGGACG GCCTCGCCAG CATCTCCTGT 
     2221 CACTGACGAG AAGGGCGCAG AAACATCGGC TGAGGGAGCT CAAGATTCAA GTCAAAGAAT 
     2281 TTGCTGACAA AGAAGAAGGT GGAGATGTGA AGGCTGTCTG CTTGACATTG TTTCTCCTGG 
     2341 CACTGAGGGC GAGGAATGAG CACAGGCAAG CTGATGAATT AGAGGCCATC ATGCAAGGCA 
     2401 GGGGCTCCGG GCTTCAACCA GCTGTTTGCT TGGCCATCCG TGTCAATACC TTCCTCAGCT 
     2461 GTAGCCAATA CCATAAGATG TACAGGACTG TGAAAGCTAT CACTGGGAGG CAGATTTTTC 
     2521 AACCTTTGCA TGCTCTTCGG AATGCCGAGA AAGTCCTTCT GCCAGGCTAC CATCCCTTTG 
     2581 AGTGGCAGCC CCCACTGAAG AATGTGTCCT CCAGAACTGA TGTTGGAATT ATTGATGGGC 
     2641 TGTCTGGACT TGCCTCCTCT GTGGATGAGT ACCCAGTAGA TACCATTGCG AAGAGGTTCC 
     2701 GCTACGACTC TGCTTTGGTG TCTGCTTTGA TGGACATGGA AGAAGACATC TTGGAAGGCA 
     2761 TGAGATCCCA AGATCTTGAT GACTACCTGA ATGGTCCCTT CACAGTGGTG GTAAAGGAGT 
     2821 CTTGCGATGG AATGGGGGAT GTGAGTGAGA AGCACGGGAG TGGGCCCGCA GTTCCAGAAA 
     2881 AGGCCGTTCG TTTCTCTTTC ACAGTCATGA GAATTACGAT AGAGCATGGT TCACAGAACG 
     2941 TGAAGGTGTT TGAGGAACCC AAGCCCAATT CTGAACTGTG TTGCAAGCCG TTGTGTCTTA 
     3001 TGCTGGCAGA TGAGTCTGAC CATGAGACCC TTACTGCTAT TCTAAGCCCC CTCATTGCCG 
     3061 AGAGGGAGGC CATGAAGAGC AGTGAATTAA CGCTGGAGAT GGGAGGCATC CCCAGGACTT 
     3121 TTAAATTCAT CTTCAGGGGC ACTGGATACG ATGAAAAACT TGTCCGGGAA GTAGAAGGCT 
     3181 TGGAAGCTTC TGGCTCAGTC TACATCTGTA CACTCTGTGA CACCACCCGT TTGGAAGCCT 
     3241 CTCAGAATCT TGTCTTCCAC TCCATAACCA GAAGCCACGC CGAGAACCTG CAGCGCTATG 
     3301 AGGTCTGGCG GTCCAATCCG TATCATGAGT CCGTGGAAGA GCTCCGGGAC CGGGTGAAAG 
     3361 GGGTCTCTGC CAAACCTTTC ATCGAGACAG TCCCTTCCAT AGATGCGCTT CACTGTGACA 
     3421 TTGGCAATGC AGCTGAATTC TATAAGATTT TCCAGCTGGA GATAGGGGAA GTGTATAAAC 
     3481 ATCCCAATGC CTCTAAAGAG GAAAGGAAGA GATGGCAGGC CACGCTGGAC AAACATCTCC 
     3541 GGAAAAGGAT GAACTTAAAA CCAATCATGA GGATGAATGG CAACTTTGCC CGGAAGCTTA 
     3601 TGACCCAAGA GACTGTAGAC GCAGTTTGTG AGTTAATTCC TTCTGAGGAG AGGCATGAAG 
     3661 CTCTCAGGGA GCTCATGGAC CTTTACCTGA AGATGAAACC CGTGTGGCGC TCTTCATGTC 
     3721 CCGCTAAAGA GTGTCCAGAG TCCCTCTGTC AGTACAGTTT CAACTCACAG CGTTTCGCGG 
     3781 AACTCCTCTC CACCAAGTTC AAATATAGAT ACGAGGGCAA AATCACCAAT TACTTTCACA 
     3841 AAACCTTGGC ACATGTCCCT GAAATTATTG AAAGGGATGG CTCTATCGGG GCCTGGGCAA 
     3901 GTGAGGGAAA TGAATCGGGT AACAAGCTGT TTAGACGGTT TCGGAAAATG AATGCCAGGC 
     3961 AGTCCAAGTG CTATGAGATG GAAGATGTCC TGAAACATCA CTGGCTGTAT ACTTCAAAAT 
     4021 ACCTCCAGAA GTTTATGAAT GCTCATAACG CGTTAAAAAG CTCTGGGTTT ACCATGAACT 
     4081 CAAAGGAGAC CTTAGGGGAC CCTTTGGGCA TTGAGGACTC TCTGGAAAGC CAAGATTCAA 
     4141 TGGAGTTTAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//