LOCUS       MR227102                7920 bp    DNA     circular  SYN 
DEFINITION  Nod2 (Myc-DDK-tagged) - Mouse nucleotide-binding oligomerization domain containing 2 (Nod2)
ACCESSION   NM_145857
VERSION     NM_145857.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4077..4168 
                     /label="Myc-DDK"  
     misc_feature    4198..4783 
                     /label="PolyA signal"  
     misc_feature    4255..4274 
                     /label="Restriction Enzyme"  
     misc_feature    5059..5731 
                     /label="ColE1"  
     misc_feature    6161..6955 
                     /label="Kan/Neo"  
     misc_feature    6990..7292 
                     /label="SV40 ori"  
     misc_feature    7378..7855 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4059..4065 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4059 
                     /label="Nod2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCTCACAG GAAGAGTTCC AGGCACAGAG GAGCCAGCTG GTGGCACTGC 
     1081 TGATCTCAGG GTCCTTGGAG GGCTTTGAGA GCATCTTAGA CTGGCTGCTG TCTTGGGATG 
     1141 TGCTCTCCAG GGAGGACTAT GAGGGCCTCA GCCTCCCTGG CCAACCTCTC TCCCATTCTG 
     1201 CCAGGCGTCT GCTGGACACA GTCTGGAACA AGGGTGTTTG GGGCTGTCAG AAGCTCCTCG 
     1261 AAGCTGTGCA GGAGGCACAG GCCAACAGTC ATACCTTTGA ACTGTATGGG TCCTGGGACA 
     1321 CTCACTCCCT TCATCCAACC AGAGACCTGC AGAGTCACCG GCCAGCCATT GTGAGGAGAC 
     1381 TCTACAACCA TGTAGAAGCC ATGCTGGAGC TGGCAAGGGA GGGGGGGTTT CTGAGCCAGT 
     1441 ACGAGTGTGA GGAGATCAGG CTGCCAATCT TCACGTCGTC TCAGAGGGCA AGAAGGCTGC 
     1501 TTGATCTCGC TGCGGTGAAG GCCAATGGAC TGGCTGCCTT CCTTCTACAG CACGTCAGGG 
     1561 AACTACCAGC TCCACTGCCT CTGCCTTACG AGGCTGCTGA GTGTCAGAAG TTCATATCTA 
     1621 AGCTGAGGAC CATGGTGTTG ACTCAGTCTC GCTTCCTCAG TACTTACGAT GGGTCGGAGA 
     1681 ATCTTTGCCT GGAGGATATA TACACGGAGA ACATCTTGGA GCTGCAGACT GAAGTGGGCA 
     1741 CAGCCGGGGC CTTGCAGAAG AGCCCTGCCA TCCTGGGCCT GGAGGACCTC TTTGATACCC 
     1801 ATGGTCACCT GAACAGAGAT GCCGACACCA TACTGGTGGT GGGCGAAGCA GGCAGTGGCA 
     1861 AGAGCACTCT TCTGCAGCGT TTGCACCTGC TGTGGGCAAC AGGGAGGAGC TTCCAGGAGT 
     1921 TTCTCTTCAT TTTCCCATTC AGCTGCCGAC AGTTGCAATG CGTGGCCAAA CCGCTGTCCC 
     1981 TGAGGACGCT GCTCTTTGAG CACTGCTGCT GGCCTGATGT CGCTCAGGAC GATGTCTTCC 
     2041 AGTTCCTTCT TGACCATCCT GACCGTGTCC TGTTAACCTT TGATGGCTTG GACGAGTTCA 
     2101 AGTTCCGGTT CACCGACCGG GAGCGCCACT GCTCTCCAAT TGACCCCACG TCAGTCCAGA 
     2161 CTCTGCTCTT CAACCTTCTC CAGGGGAACC TGCTGAAGAA TGCCTGCAAG GTGCTGACCA 
     2221 GCCGTCCGGA TGCTGTGTCA GCGCTCCTCA GGAAGTTCGT TCGCACAGAG TTGCAACTGA 
     2281 AGGGCTTCTC TGAAGAGGGC ATCCAACTGT ACCTGAGAAA GCACCACCGG GAACCTGGGG 
     2341 TGGCAGACCG CCTCATCCAG CTGATCCAAG CCACCTCAGC CCTGCATGGT TTGTGCCACC 
     2401 TCCCTGTCTT CTCTTGGATG GTGTCCAGAT GCCACCGGGA ACTTCTGCTG CAGAACAGGG 
     2461 GCTTCCCAAC AACCAGCACG GACATGTACC TCCTGATCCT GCAGCATTTC CTGCTGCATG 
     2521 CCTCCCCTCC GGACTCCTCC CCACTCGGTC TGGGACCTGG ACTCCTCCAA AGCCGGCTCT 
     2581 CCACCCTCCT GCACCTTGGC CACCTGGCTC TCCGGGGCCT GGCCATGAGC TGCTATGTGT 
     2641 TCTCAGCCCA GCAGCTCCAG GCAGCTCAGG TTGACTCTGA TGATATTTCT CTTGGTTTCC 
     2701 TGGTACGTGC CCAAAGTAGT GTGCCCGGGA GCAAGGCGCC CCTGGAGTTC CTGCACATTA 
     2761 CCTTCCAGTG CTTTTTTGCC GCTTTCTACT TGGCTGTCAG TGCTGACACA TCGGTGGCCT 
     2821 CTCTCAAGCA CCTTTTCAGC TGTGGCCGGC TGGGCAGCTC ACTGCTGGGA AGGCTGCTGC 
     2881 CCAACCTGTG TATCCAGGGC TCCAGAGTCA AGAAGGGCAG CGAAGCAGCC CTGCTGCAGA 
     2941 AGGCTGAGCC ACACAACCTG CAAATCACAG CAGCCTTCCT AGCAGGTCTG TTGTCCCAGC 
     3001 AGCATCGGGA CCTGTTGGCT GCATGCCAGG TCTCCGAGAG GGTACTGCTC CAGCGTCAGG 
     3061 CACGTGCCCG CTCGTGTCTG GCCCACAGCC TCCGCGAGCA CTTCCATTCC ATCCCGCCTG 
     3121 CCGTGCCCGG TGAGACCAAG AGCATGCATG CTATGCCGGG CTTCATTTGG CTCATCCGTA 
     3181 GCCTGTACGA GATGCAGGAG GAGCAGTTGG CCCAGGAGGC TGTCCGTCGC TTGGACATCG 
     3241 GGCACCTGAA GTTGACATTT TGCAGAGTGG GCCCTGCAGA GTGTGCTGCA CTGGCCTTTG 
     3301 TACTGCAACA TCTCCAGCGG CCTGTGGCCC TACAGCTGGA TTACAACTCT GTGGGAGATG 
     3361 TTGGAGTGGA ACAGCTGCGA CCGTGCCTTG GGGTCTGCAC AGCTCTGTAT TTGCGAGATA 
     3421 ACAATATCTC AGACCGAGGT GCCCGCACGC TGGTTGAGTG TGCTCTTCGC TGTGAGCAGC 
     3481 TGCAGAAACT AGCTCTCTTC AACAACAAAC TCACGGATGC GTGCGCCTGC TCCATGGCCA 
     3541 AGCTCCTTGC ACACAAGCAG AACTTCTTGT CCCTGAGGGT GGGGAACAAT CACATCACAG 
     3601 CCGCTGGAGC CGAGGTGCTG GCCCAGGGAC TCAAGAGCAA CACCTCCCTG AAGTTCCTGG 
     3661 GGTTCTGGGG CAACAGCGTG GGTGATAAGG GCACCCAAGC CCTGGCTGAA GTTGTAGCCG 
     3721 ACCACCAGAA CCTAAAGTGG CTCAGCCTGG TAGGAAACAA CATTGGCAGC ATGGGTGCCG 
     3781 AAGCCCTAGC ACTGATGCTG GAGAAGAACA AGTCACTAGA GGAACTCTGC CTAGAGGAAA 
     3841 ACCATATCTG TGACGAAGGG GTATATTCTC TCGCAGAAGG ACTCAAGAGA AATTCAACTT 
     3901 TGAAATTCTT GAAACTGTCC AACAATGGCA TCACCTACCG GGGTGCAGAA GCCCTCCTGC 
     3961 AGGCCCTCAG CAGGAACAGT GCCATTCTGG AGGTTTGGCT TCGAGGGAAC ACATTCTCTT 
     4021 TGGAGGAAAT CCAAACACTG AGCTCCAGGG ACGCCAGACT CTTGTTGACG CGTACGCGGC 
     4081 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4141 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4201 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4261 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4321 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4381 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4441 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4501 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4561 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4621 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4681 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4741 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4801 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4861 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4921 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4981 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5041 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5101 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5161 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5221 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5281 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5341 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5401 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5461 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5521 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5581 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5641 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5701 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5761 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5821 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5881 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5941 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6001 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6061 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6121 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6181 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6241 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6301 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6361 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6421 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6481 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6541 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6601 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6661 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6721 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6781 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6841 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6901 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6961 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7021 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7081 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7141 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7261 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7321 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7381 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7441 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7501 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7561 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7621 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7681 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7741 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7801 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7861 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//